Effect of culture pH on recombinant antibody production by a new human cell line, F2N78, grown in suspension at 33.0 °C and 37.0 °C

The human host cell line, F2N78, is a new somatic hybrid cell line designed for therapeutic antibody production. To verify its potential as a human host cell line, recombinant F2N78 cells that produce antibody against rabies virus (rF2N78) were cultivated at different culture pH (6.8, 7.0, 7.2, 7.4,...

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Veröffentlicht in:	Applied microbiology and biotechnology 2013-06, Vol.97 (12), p.5283-5291
Hauptverfasser:	Seo, Jin Seok, Kim, Yeon Jung, Cho, Jong Moon, Baek, Eric, Lee, Gyun Min
Format:	Artikel
Sprache:	eng
Schlagworte:	Ammonia Analysis Antibodies Antibodies, Viral - genetics Antibodies, Viral - metabolism Bats Biomedical and Life Sciences Biotechnological Products and Process Engineering Biotechnology Cell Culture Techniques Cell growth Cell Line Cell Proliferation - drug effects Cell Proliferation - radiation effects Cells Cellular biology Culture Media - chemistry Glucose Glycosylation Glycosylation - drug effects Glycosylation - radiation effects Health aspects Humans Hydrogen-Ion Concentration Life Sciences Mass spectrometry Metabolism - drug effects Metabolism - radiation effects Microbial Genetics and Genomics Microbiology Monoclonal antibodies Productivity Rabies Rabies virus - immunology Recombinant Proteins - genetics Recombinant Proteins - metabolism Scientific imaging Studies Suspensions Technology, Pharmaceutical - methods Temperature Viral antibodies
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