Characterization of a novel β-agarase from an agar-degrading bacterium Catenovulum sp. X3

An agar-degrading bacterium, Catenovulum sp. X3, was isolated from the seawater of Shantou, China. A novel β-agarase gene agaXa was cloned from the strain Catenovulum sp. X3. The gene agaXa consists of 1,590 bp and encodes a protein of 529 amino acids, with only 40 % amino acid sequence identity wit...

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Veröffentlicht in:	Applied microbiology and biotechnology 2013-06, Vol.97 (11), p.4907-4915
Hauptverfasser:	Xie, Wei, Lin, Bokun, Zhou, Zhengrong, Lu, Guoyong, Lun, Jingsheng, Xia, Changyan, Li, Shengkang, Hu, Zhong
Format:	Artikel
Sprache:	eng
Schlagworte:	Agar - metabolism Alteromonadaceae - enzymology Alteromonadaceae - genetics Alteromonadaceae - isolation & purification Amino acids Bacteria Biomedical and Life Sciences Biotechnologically Relevant Enzymes and Proteins Biotechnology Chemical properties China Cloning, Molecular DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Electrophoresis, Polyacrylamide Gel Enzyme Stability Enzymes Glycoside Hydrolases - chemistry Glycoside Hydrolases - genetics Glycoside Hydrolases - isolation & purification Glycoside Hydrolases - metabolism Hydrogen-Ion Concentration Hydrolases Kinetics Life Sciences Microbial Genetics and Genomics Microbiology Molecular Sequence Data Molecular Weight RNA, Ribosomal, 16S - genetics Sea-water Seawater - microbiology Sequence Analysis, DNA Sequence Homology, Amino Acid Substrate Specificity Sulfates Surface active agents Temperature
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