Free radical scavenging ability and structure of a 90-kDa protein from the scallop shell
We have previously reported that the scallop shell extract possesses free radical scavenging activity. In this study, we isolated a glycoprotein with a molecular weight of approximately 90 kDa (named 90-kDa protein) which showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and superoxide anion radic...
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| Veröffentlicht in: | Fisheries science 2013-05, Vol.79 (3), p.495-502 |
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| creator | Mitsuhashi, Takuya Ono, Kohji Fukuda, Manabu Hasegawa, Yasushi |
| description | We have previously reported that the scallop shell extract possesses free radical scavenging activity. In this study, we isolated a glycoprotein with a molecular weight of approximately 90 kDa (named 90-kDa protein) which showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and superoxide anion radical scavenging, reducing, and ferrous ion-chelating activities. Amino acid composition analysis showed that the 90-kDa protein was rich in Asx (Asp or Asn), Ser and Gly residues. A BLAST search of partial amino acid sequences determined by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry revealed that the 90-kDa protein is a novel protein. The 90-kDa protein is a yellow protein that contains fluorescent substances. To the best of our knowledge, this is the first report of a radical scavenging protein from the scallop shell. |
| doi_str_mv | 10.1007/s12562-013-0616-7 |
| format | Article |
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In this study, we isolated a glycoprotein with a molecular weight of approximately 90 kDa (named 90-kDa protein) which showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and superoxide anion radical scavenging, reducing, and ferrous ion-chelating activities. Amino acid composition analysis showed that the 90-kDa protein was rich in Asx (Asp or Asn), Ser and Gly residues. A BLAST search of partial amino acid sequences determined by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry revealed that the 90-kDa protein is a novel protein. The 90-kDa protein is a yellow protein that contains fluorescent substances. 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In this study, we isolated a glycoprotein with a molecular weight of approximately 90 kDa (named 90-kDa protein) which showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and superoxide anion radical scavenging, reducing, and ferrous ion-chelating activities. Amino acid composition analysis showed that the 90-kDa protein was rich in Asx (Asp or Asn), Ser and Gly residues. A BLAST search of partial amino acid sequences determined by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry revealed that the 90-kDa protein is a novel protein. The 90-kDa protein is a yellow protein that contains fluorescent substances. To the best of our knowledge, this is the first report of a radical scavenging protein from the scallop shell.</abstract><cop>Japan</cop><pub>Springer Japan</pub><doi>10.1007/s12562-013-0616-7</doi><tpages>8</tpages></addata></record> |
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| subjects | Amino acids Apoptosis Biomedical and Life Sciences Cell death Cellulose Chromatography Fish & Wildlife Biology & Management Fishery sciences Food Science Free radicals Freshwater & Marine Ecology Hemodialysis Ionization Life Sciences Marine Mass spectrometry Molecular weight Original Article Potassium Proteins Shellfish Shells Skin Statistical analysis |
| title | Free radical scavenging ability and structure of a 90-kDa protein from the scallop shell |
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