Characterization of lead-resistant river isolate Enterococcus faecalis and assessment of its multiple metal and antibiotic resistance
Contamination of surface waters has a direct impact on the public health of entire communities. Microorganisms inhabiting contaminated surface waters have developed mechanisms of coping with a variety of toxic metals and drugs. Investigations were carried out to isolate and identify lead-resistant b...
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| Veröffentlicht in: | Environmental monitoring and assessment 2013-06, Vol.185 (6), p.5285-5293 |
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| creator | Aktan, Yasin Tan, Sema Icgen, Bulent |
| description | Contamination of surface waters has a direct impact on the public health of entire communities. Microorganisms inhabiting contaminated surface waters have developed mechanisms of coping with a variety of toxic metals and drugs. Investigations were carried out to isolate and identify lead-resistant bacteria from the river Kızılırmak along the city of Kırıkkale, Turkey. Of the 33 lead-resistant isolates, one isolate with a minimal inhibitory concentration of 1,200 mg L
−1
was isolated and identified as
Enterococcus faecalis
by using biochemical tests and 16S rRNA sequencing. Lead-resistant
E. faecalis
isolate was found out to be resistant to other heavy metals like aluminum, lithium, barium, chromium, iron, silver, tin, nickel, zinc, and strontium and to drugs like amikacin, aztreonam, and gentamicin.
E. faecalis
harbored four plasmids with the molecular sizes of 1.58, 3.06, 22.76, and 28.95 kb. Plasmid profile analyses of cured derivatives revealed that the lead resistance ability of
E. faecalis
was still existing despite the elimination of all the plasmids. Moreover, the antibiotic resistance pattern of the cured derivatives did not demonstrate any change from the parental strain. Our findings indicated that the lead resistance genes of
E. faecalis
were located on the chromosomal DNA rather than the plasmid. |
| doi_str_mv | 10.1007/s10661-012-2945-x |
| format | Article |
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−1
was isolated and identified as
Enterococcus faecalis
by using biochemical tests and 16S rRNA sequencing. Lead-resistant
E. faecalis
isolate was found out to be resistant to other heavy metals like aluminum, lithium, barium, chromium, iron, silver, tin, nickel, zinc, and strontium and to drugs like amikacin, aztreonam, and gentamicin.
E. faecalis
harbored four plasmids with the molecular sizes of 1.58, 3.06, 22.76, and 28.95 kb. Plasmid profile analyses of cured derivatives revealed that the lead resistance ability of
E. faecalis
was still existing despite the elimination of all the plasmids. Moreover, the antibiotic resistance pattern of the cured derivatives did not demonstrate any change from the parental strain. Our findings indicated that the lead resistance genes of
E. faecalis
were located on the chromosomal DNA rather than the plasmid.</description><identifier>ISSN: 0167-6369</identifier><identifier>EISSN: 1573-2959</identifier><identifier>DOI: 10.1007/s10661-012-2945-x</identifier><identifier>PMID: 23079796</identifier><identifier>CODEN: EMASDH</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Adaptation, Physiological ; Aluminum ; Analysis ; Animal, plant and microbial ecology ; Anti-Bacterial Agents - analysis ; Anti-Bacterial Agents - toxicity ; Antibiotic resistance ; Antibiotics ; Antimicrobial agents ; Applied ecology ; Atmospheric Protection/Air Quality Control/Air Pollution ; Bacteria ; Barium ; Biochemical tests ; Biological and medical sciences ; Cadmium ; Chromium ; Conservation, protection and management of environment and wildlife ; Drug resistance ; Drug Resistance, Bacterial - physiology ; Earth and Environmental Science ; Ecology ; Ecotoxicology ; Enterococcus faecalis ; Enterococcus faecalis - isolation & purification ; Enterococcus faecalis - physiology ; Environment ; Environmental Management ; Environmental Monitoring ; Food contamination & poisoning ; Fundamental and applied biological sciences. Psychology ; Genes ; Health ; Heavy metals ; Lead ; Lead - analysis ; Lead - toxicity ; Lithium ; Metals, Heavy - analysis ; Metals, Heavy - toxicity ; Microorganisms ; Monitoring/Environmental Analysis ; Morphology ; Nickel ; Pathogens ; Plasmids ; Public health ; Rivers ; Rivers - chemistry ; Rivers - microbiology ; Silver ; Strontium ; Studies ; Surface water ; Turkey ; Water Pollutants, Chemical - analysis ; Water Pollutants, Chemical - toxicity</subject><ispartof>Environmental monitoring and assessment, 2013-06, Vol.185 (6), p.5285-5293</ispartof><rights>Springer Science+Business Media Dordrecht 2012</rights><rights>2014 INIST-CNRS</rights><rights>Springer Science+Business Media Dordrecht 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c435t-c6b4873692f975701e46d44785d2d3e1b055e9da11c63899354fb5616aead84c3</citedby><cites>FETCH-LOGICAL-c435t-c6b4873692f975701e46d44785d2d3e1b055e9da11c63899354fb5616aead84c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10661-012-2945-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10661-012-2945-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27644268$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23079796$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aktan, Yasin</creatorcontrib><creatorcontrib>Tan, Sema</creatorcontrib><creatorcontrib>Icgen, Bulent</creatorcontrib><title>Characterization of lead-resistant river isolate Enterococcus faecalis and assessment of its multiple metal and antibiotic resistance</title><title>Environmental monitoring and assessment</title><addtitle>Environ Monit Assess</addtitle><addtitle>Environ Monit Assess</addtitle><description>Contamination of surface waters has a direct impact on the public health of entire communities. Microorganisms inhabiting contaminated surface waters have developed mechanisms of coping with a variety of toxic metals and drugs. Investigations were carried out to isolate and identify lead-resistant bacteria from the river Kızılırmak along the city of Kırıkkale, Turkey. Of the 33 lead-resistant isolates, one isolate with a minimal inhibitory concentration of 1,200 mg L
−1
was isolated and identified as
Enterococcus faecalis
by using biochemical tests and 16S rRNA sequencing. Lead-resistant
E. faecalis
isolate was found out to be resistant to other heavy metals like aluminum, lithium, barium, chromium, iron, silver, tin, nickel, zinc, and strontium and to drugs like amikacin, aztreonam, and gentamicin.
E. faecalis
harbored four plasmids with the molecular sizes of 1.58, 3.06, 22.76, and 28.95 kb. Plasmid profile analyses of cured derivatives revealed that the lead resistance ability of
E. faecalis
was still existing despite the elimination of all the plasmids. Moreover, the antibiotic resistance pattern of the cured derivatives did not demonstrate any change from the parental strain. Our findings indicated that the lead resistance genes of
E. faecalis
were located on the chromosomal DNA rather than the plasmid.</description><subject>Adaptation, Physiological</subject><subject>Aluminum</subject><subject>Analysis</subject><subject>Animal, plant and microbial ecology</subject><subject>Anti-Bacterial Agents - analysis</subject><subject>Anti-Bacterial Agents - toxicity</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Applied ecology</subject><subject>Atmospheric Protection/Air Quality Control/Air Pollution</subject><subject>Bacteria</subject><subject>Barium</subject><subject>Biochemical tests</subject><subject>Biological and medical sciences</subject><subject>Cadmium</subject><subject>Chromium</subject><subject>Conservation, protection and management of environment and wildlife</subject><subject>Drug resistance</subject><subject>Drug Resistance, Bacterial - physiology</subject><subject>Earth and Environmental Science</subject><subject>Ecology</subject><subject>Ecotoxicology</subject><subject>Enterococcus faecalis</subject><subject>Enterococcus faecalis - isolation & purification</subject><subject>Enterococcus faecalis - physiology</subject><subject>Environment</subject><subject>Environmental Management</subject><subject>Environmental Monitoring</subject><subject>Food contamination & poisoning</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Health</subject><subject>Heavy metals</subject><subject>Lead</subject><subject>Lead - analysis</subject><subject>Lead - toxicity</subject><subject>Lithium</subject><subject>Metals, Heavy - analysis</subject><subject>Metals, Heavy - toxicity</subject><subject>Microorganisms</subject><subject>Monitoring/Environmental Analysis</subject><subject>Morphology</subject><subject>Nickel</subject><subject>Pathogens</subject><subject>Plasmids</subject><subject>Public health</subject><subject>Rivers</subject><subject>Rivers - chemistry</subject><subject>Rivers - microbiology</subject><subject>Silver</subject><subject>Strontium</subject><subject>Studies</subject><subject>Surface water</subject><subject>Turkey</subject><subject>Water Pollutants, Chemical - analysis</subject><subject>Water Pollutants, Chemical - toxicity</subject><issn>0167-6369</issn><issn>1573-2959</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kUtv1TAQhS1ERS-FH8AGWUJIbFLsxI94ia7KQ6rUDawjx5mAqyS-eBzUsud_d6rcAkJiZY38zZmZcxh7IcW5FMK-RSmMkZWQdVU7paubR2wntW2o0u4x2wlpbGUa407ZU8RrIYSzyj1hp3UjrLPO7Niv_TeffSiQ409fYlp4GvkEfqgyYMTil8Jz_AGZR0yTL8AvFoJTSCGsyEcPwU8RuV8G7hEBcQZqIZFYkM_rVOJhAj5D8dMGLSX2MZUY-MOEAM_YyegnhOfH94x9eX_xef-xurz68Gn_7rIKqtGlCqZXraV76tFZbYUEZQalbKuHemhA9kJrcIOXMpimda7Rauy1kcbTQa0KzRl7s-kecvq-ApZujhhgmvwCacVONprEWzKT0Ff_oNdpzQttR5SyRtXaaKLkRoWcEDOM3SHH2efbToruPqNuy6ijjLr7jLob6nl5VF77GYbfHQ-hEPD6CHgkd8dMFkX8w9FwVZuWuHrjkL6Wr5D_WvG_0-8AwIKroA</recordid><startdate>20130601</startdate><enddate>20130601</enddate><creator>Aktan, Yasin</creator><creator>Tan, Sema</creator><creator>Icgen, Bulent</creator><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QH</scope><scope>7QL</scope><scope>7SN</scope><scope>7ST</scope><scope>7T7</scope><scope>7TG</scope><scope>7TN</scope><scope>7U7</scope><scope>7UA</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H97</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>KL.</scope><scope>L.-</scope><scope>L.G</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>P64</scope><scope>PATMY</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>SOI</scope><scope>7T2</scope><scope>7TV</scope><scope>7U2</scope></search><sort><creationdate>20130601</creationdate><title>Characterization of lead-resistant river isolate Enterococcus faecalis and assessment of its multiple metal and antibiotic resistance</title><author>Aktan, Yasin ; Tan, Sema ; Icgen, Bulent</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-c6b4873692f975701e46d44785d2d3e1b055e9da11c63899354fb5616aead84c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adaptation, Physiological</topic><topic>Aluminum</topic><topic>Analysis</topic><topic>Animal, plant and microbial ecology</topic><topic>Anti-Bacterial Agents - analysis</topic><topic>Anti-Bacterial Agents - toxicity</topic><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Antimicrobial agents</topic><topic>Applied ecology</topic><topic>Atmospheric Protection/Air Quality Control/Air Pollution</topic><topic>Bacteria</topic><topic>Barium</topic><topic>Biochemical tests</topic><topic>Biological and medical sciences</topic><topic>Cadmium</topic><topic>Chromium</topic><topic>Conservation, protection and management of environment and wildlife</topic><topic>Drug resistance</topic><topic>Drug Resistance, Bacterial - physiology</topic><topic>Earth and Environmental Science</topic><topic>Ecology</topic><topic>Ecotoxicology</topic><topic>Enterococcus faecalis</topic><topic>Enterococcus faecalis - isolation & purification</topic><topic>Enterococcus faecalis - physiology</topic><topic>Environment</topic><topic>Environmental Management</topic><topic>Environmental Monitoring</topic><topic>Food contamination & poisoning</topic><topic>Fundamental and applied biological sciences. 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Microorganisms inhabiting contaminated surface waters have developed mechanisms of coping with a variety of toxic metals and drugs. Investigations were carried out to isolate and identify lead-resistant bacteria from the river Kızılırmak along the city of Kırıkkale, Turkey. Of the 33 lead-resistant isolates, one isolate with a minimal inhibitory concentration of 1,200 mg L
−1
was isolated and identified as
Enterococcus faecalis
by using biochemical tests and 16S rRNA sequencing. Lead-resistant
E. faecalis
isolate was found out to be resistant to other heavy metals like aluminum, lithium, barium, chromium, iron, silver, tin, nickel, zinc, and strontium and to drugs like amikacin, aztreonam, and gentamicin.
E. faecalis
harbored four plasmids with the molecular sizes of 1.58, 3.06, 22.76, and 28.95 kb. Plasmid profile analyses of cured derivatives revealed that the lead resistance ability of
E. faecalis
was still existing despite the elimination of all the plasmids. Moreover, the antibiotic resistance pattern of the cured derivatives did not demonstrate any change from the parental strain. Our findings indicated that the lead resistance genes of
E. faecalis
were located on the chromosomal DNA rather than the plasmid.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>23079796</pmid><doi>10.1007/s10661-012-2945-x</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-6369 |
| ispartof | Environmental monitoring and assessment, 2013-06, Vol.185 (6), p.5285-5293 |
| issn | 0167-6369 1573-2959 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Adaptation, Physiological Aluminum Analysis Animal, plant and microbial ecology Anti-Bacterial Agents - analysis Anti-Bacterial Agents - toxicity Antibiotic resistance Antibiotics Antimicrobial agents Applied ecology Atmospheric Protection/Air Quality Control/Air Pollution Bacteria Barium Biochemical tests Biological and medical sciences Cadmium Chromium Conservation, protection and management of environment and wildlife Drug resistance Drug Resistance, Bacterial - physiology Earth and Environmental Science Ecology Ecotoxicology Enterococcus faecalis Enterococcus faecalis - isolation & purification Enterococcus faecalis - physiology Environment Environmental Management Environmental Monitoring Food contamination & poisoning Fundamental and applied biological sciences. Psychology Genes Health Heavy metals Lead Lead - analysis Lead - toxicity Lithium Metals, Heavy - analysis Metals, Heavy - toxicity Microorganisms Monitoring/Environmental Analysis Morphology Nickel Pathogens Plasmids Public health Rivers Rivers - chemistry Rivers - microbiology Silver Strontium Studies Surface water Turkey Water Pollutants, Chemical - analysis Water Pollutants, Chemical - toxicity |
| title | Characterization of lead-resistant river isolate Enterococcus faecalis and assessment of its multiple metal and antibiotic resistance |
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