miR-573 regulates melanoma progression by targeting the melanoma cell adhesion molecule
Melanoma is a malignant tumor of the melanocytes. microRNAs (miRNAs) are emerging as important regulators of cancer-related processes. A thorough understanding of miRNAs in melanoma progression is important for developing new therapeutic targets. miRNA expression was detected by quantitative PCR. In...
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| Veröffentlicht in: | Oncology reports 2013-07, Vol.30 (1), p.520-526 |
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| creator | WANG, HE-FEI CHEN, HONG MA, MIN-WANG WANG, JI-AN TANG, TING-TING NI, LAI-SHENG YU, JIN-LING LI, YU-ZHEN BAI, BING-XUE |
| description | Melanoma is a malignant tumor of the melanocytes. microRNAs (miRNAs) are emerging as important regulators of cancer-related processes. A thorough understanding of miRNAs in melanoma progression is important for developing new therapeutic targets. miRNA expression was detected by quantitative PCR. In vitro, MTT assay, colony formation assay, invasion assay and flow cytometry analysis were performed to test the effect of miR-573 on melanoma cells. The effect of miR-573 in vivo was validated using a murine xenograft model. Using quantitative PCR, we found that the expression levels of miR-573 were lower in melanoma tissues and cell lines compared to normal skin tissues. miR-573 upregulation inhibited melanoma cell proliferation and invasion, and overexpression of melanoma cell adhesion molecule (MCAM) could alleviate the effect of miR-573 on melanoma cells. In vivo, miR-573 overexpression groups showed lower rates of tumor growth compared with the control group. In conclusion, our results demonstrate that the elevated MCAM expression due to miR-573 reduction is essential in melanoma initiation and progression. |
| doi_str_mv | 10.3892/or.2013.2451 |
| format | Article |
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A thorough understanding of miRNAs in melanoma progression is important for developing new therapeutic targets. miRNA expression was detected by quantitative PCR. In vitro, MTT assay, colony formation assay, invasion assay and flow cytometry analysis were performed to test the effect of miR-573 on melanoma cells. The effect of miR-573 in vivo was validated using a murine xenograft model. Using quantitative PCR, we found that the expression levels of miR-573 were lower in melanoma tissues and cell lines compared to normal skin tissues. miR-573 upregulation inhibited melanoma cell proliferation and invasion, and overexpression of melanoma cell adhesion molecule (MCAM) could alleviate the effect of miR-573 on melanoma cells. In vivo, miR-573 overexpression groups showed lower rates of tumor growth compared with the control group. In conclusion, our results demonstrate that the elevated MCAM expression due to miR-573 reduction is essential in melanoma initiation and progression.</description><identifier>ISSN: 1021-335X</identifier><identifier>EISSN: 1791-2431</identifier><identifier>DOI: 10.3892/or.2013.2451</identifier><identifier>PMID: 23670160</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Animals ; Apoptosis ; CD146 Antigen - biosynthesis ; CD146 Antigen - metabolism ; Cell adhesion & migration ; Cell Line, Tumor ; Cell Proliferation ; Disease Progression ; Gene expression ; Gene Expression Regulation, Neoplastic ; Humans ; Melanoma ; Melanoma - genetics ; Melanoma - metabolism ; melanoma cell adhesion molecule ; Mice ; microRNA ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miR-573 ; Neoplasm Invasiveness - genetics ; Neoplasm Transplantation ; Plasmids ; Proteins ; Skin cancer ; Studies ; Tumors ; Up-Regulation ; Xenograft Model Antitumor Assays</subject><ispartof>Oncology reports, 2013-07, Vol.30 (1), p.520-526</ispartof><rights>Copyright © 2013, Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-4fdf7b34e77c3f5346efb0013b3ef7a7609d8c47d680805206f76a0bdac3ce443</citedby><cites>FETCH-LOGICAL-c388t-4fdf7b34e77c3f5346efb0013b3ef7a7609d8c47d680805206f76a0bdac3ce443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23670160$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WANG, HE-FEI</creatorcontrib><creatorcontrib>CHEN, HONG</creatorcontrib><creatorcontrib>MA, MIN-WANG</creatorcontrib><creatorcontrib>WANG, JI-AN</creatorcontrib><creatorcontrib>TANG, TING-TING</creatorcontrib><creatorcontrib>NI, LAI-SHENG</creatorcontrib><creatorcontrib>YU, JIN-LING</creatorcontrib><creatorcontrib>LI, YU-ZHEN</creatorcontrib><creatorcontrib>BAI, BING-XUE</creatorcontrib><title>miR-573 regulates melanoma progression by targeting the melanoma cell adhesion molecule</title><title>Oncology reports</title><addtitle>Oncol Rep</addtitle><description>Melanoma is a malignant tumor of the melanocytes. microRNAs (miRNAs) are emerging as important regulators of cancer-related processes. A thorough understanding of miRNAs in melanoma progression is important for developing new therapeutic targets. miRNA expression was detected by quantitative PCR. In vitro, MTT assay, colony formation assay, invasion assay and flow cytometry analysis were performed to test the effect of miR-573 on melanoma cells. The effect of miR-573 in vivo was validated using a murine xenograft model. Using quantitative PCR, we found that the expression levels of miR-573 were lower in melanoma tissues and cell lines compared to normal skin tissues. miR-573 upregulation inhibited melanoma cell proliferation and invasion, and overexpression of melanoma cell adhesion molecule (MCAM) could alleviate the effect of miR-573 on melanoma cells. In vivo, miR-573 overexpression groups showed lower rates of tumor growth compared with the control group. In conclusion, our results demonstrate that the elevated MCAM expression due to miR-573 reduction is essential in melanoma initiation and progression.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>CD146 Antigen - biosynthesis</subject><subject>CD146 Antigen - metabolism</subject><subject>Cell adhesion & migration</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation</subject><subject>Disease Progression</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Melanoma</subject><subject>Melanoma - genetics</subject><subject>Melanoma - metabolism</subject><subject>melanoma cell adhesion molecule</subject><subject>Mice</subject><subject>microRNA</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miR-573</subject><subject>Neoplasm Invasiveness - genetics</subject><subject>Neoplasm Transplantation</subject><subject>Plasmids</subject><subject>Proteins</subject><subject>Skin cancer</subject><subject>Studies</subject><subject>Tumors</subject><subject>Up-Regulation</subject><subject>Xenograft Model Antitumor Assays</subject><issn>1021-335X</issn><issn>1791-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpd0Etr3TAQBWAREvLsLutiCJQsotuRRpbsZQlJWwgEQkK6E7I9vnGwrVvJXuTfR-7NA7rRaPExnDmMnQpYYVHK7z6sJAhcSZWLHXYoTCm4VCh20x-k4Ij5nwN2FOMzgDSgy312IFEbEBoO2ePQ3fHcYBZoPfduopgN1LvRDy7bBL8OFGPnx6x6ySYX1jR14zqbnuhT1dT3mWue6J8bfE_13NMJ22tdH-nL2zxmD9dX95e_-M3tz9-XP254jUUxcdU2ralQkTE1tjkqTW0F6ZwKqTXOaCibolam0QUUkEvQrdEOqsbVWJNSeMzOt3tT2L8zxckOXVwiuZH8HK3AXJlSCiETPfuPPvs5jCmdFSVKXaQXkrrYqjr4GAO1dhO6wYUXK8AuhVsf7FK4XQpP_Ovb0rkaqPnA7w0n8G0L4saNTdf4-GF84AgcBF8Ow1fGSode</recordid><startdate>20130701</startdate><enddate>20130701</enddate><creator>WANG, HE-FEI</creator><creator>CHEN, HONG</creator><creator>MA, MIN-WANG</creator><creator>WANG, JI-AN</creator><creator>TANG, TING-TING</creator><creator>NI, LAI-SHENG</creator><creator>YU, JIN-LING</creator><creator>LI, YU-ZHEN</creator><creator>BAI, BING-XUE</creator><general>D.A. Spandidos</general><general>Spandidos Publications UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20130701</creationdate><title>miR-573 regulates melanoma progression by targeting the melanoma cell adhesion molecule</title><author>WANG, HE-FEI ; CHEN, HONG ; MA, MIN-WANG ; WANG, JI-AN ; TANG, TING-TING ; NI, LAI-SHENG ; YU, JIN-LING ; LI, YU-ZHEN ; BAI, BING-XUE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-4fdf7b34e77c3f5346efb0013b3ef7a7609d8c47d680805206f76a0bdac3ce443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>CD146 Antigen - biosynthesis</topic><topic>CD146 Antigen - metabolism</topic><topic>Cell adhesion & migration</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation</topic><topic>Disease Progression</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Melanoma</topic><topic>Melanoma - genetics</topic><topic>Melanoma - metabolism</topic><topic>melanoma cell adhesion molecule</topic><topic>Mice</topic><topic>microRNA</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>miR-573</topic><topic>Neoplasm Invasiveness - genetics</topic><topic>Neoplasm Transplantation</topic><topic>Plasmids</topic><topic>Proteins</topic><topic>Skin cancer</topic><topic>Studies</topic><topic>Tumors</topic><topic>Up-Regulation</topic><topic>Xenograft Model Antitumor Assays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WANG, HE-FEI</creatorcontrib><creatorcontrib>CHEN, HONG</creatorcontrib><creatorcontrib>MA, MIN-WANG</creatorcontrib><creatorcontrib>WANG, JI-AN</creatorcontrib><creatorcontrib>TANG, TING-TING</creatorcontrib><creatorcontrib>NI, LAI-SHENG</creatorcontrib><creatorcontrib>YU, JIN-LING</creatorcontrib><creatorcontrib>LI, YU-ZHEN</creatorcontrib><creatorcontrib>BAI, BING-XUE</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Oncology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WANG, HE-FEI</au><au>CHEN, HONG</au><au>MA, MIN-WANG</au><au>WANG, JI-AN</au><au>TANG, TING-TING</au><au>NI, LAI-SHENG</au><au>YU, JIN-LING</au><au>LI, YU-ZHEN</au><au>BAI, BING-XUE</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>miR-573 regulates melanoma progression by targeting the melanoma cell adhesion molecule</atitle><jtitle>Oncology reports</jtitle><addtitle>Oncol Rep</addtitle><date>2013-07-01</date><risdate>2013</risdate><volume>30</volume><issue>1</issue><spage>520</spage><epage>526</epage><pages>520-526</pages><issn>1021-335X</issn><eissn>1791-2431</eissn><abstract>Melanoma is a malignant tumor of the melanocytes. microRNAs (miRNAs) are emerging as important regulators of cancer-related processes. A thorough understanding of miRNAs in melanoma progression is important for developing new therapeutic targets. miRNA expression was detected by quantitative PCR. In vitro, MTT assay, colony formation assay, invasion assay and flow cytometry analysis were performed to test the effect of miR-573 on melanoma cells. The effect of miR-573 in vivo was validated using a murine xenograft model. Using quantitative PCR, we found that the expression levels of miR-573 were lower in melanoma tissues and cell lines compared to normal skin tissues. miR-573 upregulation inhibited melanoma cell proliferation and invasion, and overexpression of melanoma cell adhesion molecule (MCAM) could alleviate the effect of miR-573 on melanoma cells. In vivo, miR-573 overexpression groups showed lower rates of tumor growth compared with the control group. In conclusion, our results demonstrate that the elevated MCAM expression due to miR-573 reduction is essential in melanoma initiation and progression.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>23670160</pmid><doi>10.3892/or.2013.2451</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Oncology reports, 2013-07, Vol.30 (1), p.520-526 |
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| subjects | Animals Apoptosis CD146 Antigen - biosynthesis CD146 Antigen - metabolism Cell adhesion & migration Cell Line, Tumor Cell Proliferation Disease Progression Gene expression Gene Expression Regulation, Neoplastic Humans Melanoma Melanoma - genetics Melanoma - metabolism melanoma cell adhesion molecule Mice microRNA MicroRNAs - genetics MicroRNAs - metabolism miR-573 Neoplasm Invasiveness - genetics Neoplasm Transplantation Plasmids Proteins Skin cancer Studies Tumors Up-Regulation Xenograft Model Antitumor Assays |
| title | miR-573 regulates melanoma progression by targeting the melanoma cell adhesion molecule |
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