Modulated Dye Retention for the Signal-On Fluorometric Determination of Acetylcholinesterase Inhibitor

A novel fluorometric assay method based on target-induced signal on was developed for acetylcholinesterase (AChE) inhibitor with obviously improved detection sensitivity. In this method, the AChE molecules catalyzed the hydrolysis of acetylthiocholine (ATCl) to form thiocholine, which in turn can sp...

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Veröffentlicht in:	Analytical chemistry (Washington) 2013-05, Vol.85 (10), p.4968-4973
Hauptverfasser:	Liao, Shuzhen, Han, Wenting, Ding, Huazhi, Xie, Dexun, Tan, Hui, Yang, Shengyuan, Wu, Zhaoyang, Shen, Guoli, Yu, Ruqin
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylcholinesterase - metabolism Acetylthiocholine - metabolism Bioassays Biocatalysis - drug effects Biosensing Techniques - methods Cholinesterase Inhibitors - analysis Cholinesterase Inhibitors - pharmacology Fluorescence Fluorescent Dyes - chemistry Fluorometry - methods Hydrolysis - drug effects Molecular chemistry Paraoxon - analysis Paraoxon - pharmacology Proteases Quenching Signal transduction
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