Lactobacillus plantarum passage through an oro-gastro-intestinal tract simulator: Carrier matrix effect and transcriptional analysis of genes associated to stress and probiosis

Dietary probiotics should reach the intestine viable and in high numbers; therefore, they should tolerate the stress associated to the gastro-intestinal (GI) environment. Indeed, all along the different GI sections, probiotics are challenged by several sources of stress, including low pH, bile and d...

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Veröffentlicht in:Microbiological research 2013-07, Vol.168 (6), p.351-359
Hauptverfasser: Bove, P., Russo, P., Capozzi, V., Gallone, A., Spano, G., Fiocco, D.
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container_end_page 359
container_issue 6
container_start_page 351
container_title Microbiological research
container_volume 168
creator Bove, P.
Russo, P.
Capozzi, V.
Gallone, A.
Spano, G.
Fiocco, D.
description Dietary probiotics should reach the intestine viable and in high numbers; therefore, they should tolerate the stress associated to the gastro-intestinal (GI) environment. Indeed, all along the different GI sections, probiotics are challenged by several sources of stress, including low pH, bile and digestive enzymes. Bacterial cells are equipped with various defense mechanisms to allow survival in hostile environments. The food matrix used to deliver beneficial bacteria may contribute to their probiotic action, e.g. by enhancing survival to stress and gut colonization. In this study, the survival of the lactic acid bacterium Lactobacillus plantarum WCFS1, a model probiotic strain, was examined in a human oro-gastric-intestinal (OGI) in vitro system, using different carrier matrices to compare protective and buffering properties. Higher survival was observed in complex and/or nutrient-rich matrices, and when potential prebiotics were added. The molecular response of L. plantarum to the OGI transit was analyzed by studying the transcriptional levels of genes involved in stress response and probiosis. The OGI steps of higher mortality corresponded to greater induction of stress genes, thus implying their involvement in adaptation to the gut environment. Plantaricins were significantly upregulated all along the different OGI sections; adhesion genes were mainly induced by gastric environment.
doi_str_mv 10.1016/j.micres.2013.01.004
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subjects Bacterial Proteins - genetics
Bacterial Proteins - metabolism
bile
digestive enzymes
food matrix
Gastrointestinal Tract - microbiology
gene expression regulation
Gene Expression Regulation, Bacterial
genes
Humans
intestines
lactic acid bacteria
Lactobacillus plantarum
Lactobacillus plantarum - genetics
Lactobacillus plantarum - physiology
Models, Biological
mortality
OGI model
plantaricins
prebiotics
Probiotics
Probiotics - metabolism
Stress and adhesion genes
stress response
Stress, Physiological
transcription (genetics)
Transcription, Genetic
Vehicle matrix
title Lactobacillus plantarum passage through an oro-gastro-intestinal tract simulator: Carrier matrix effect and transcriptional analysis of genes associated to stress and probiosis
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