Evaluation of commercial kits for the extraction and purification of viral nucleic acids from environmental and fecal samples

•We evaluate three commercial kits for extraction of viral DNA/RNA from environmental matrices.•We quantify viral genomes and estimate inhibition levels using TaqMan-based quantitative PCR.•The extraction efficiencies are comparable in samples found to have low level of inhibitors.•The MOBIO kit pro...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of virological methods 2013-07, Vol.191 (1), p.24-30
Hauptverfasser: Iker, Brandon C., Bright, Kelly R., Pepper, Ian L., Gerba, Charles P., Kitajima, Masaaki
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!