Efficient Regeneration Potential is Closely Related to Auxin Exposure Time and Catalase Metabolism During the Somatic Embryogenesis of Immature Embryos in Triticum aestivum L
Regeneration of cultured tissue is a prerequisite of Agrobacterium- and biolistic-mediated plant transformation. In this study, an efficient protocol for improving wheat (Triticum aestivum L.) immature embryo regeneration was developed. Based on the statistical analysis of embryogenic callus inducti...
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| Veröffentlicht in: | Molecular biotechnology 2013-06, Vol.54 (2), p.451-460 |
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| creator | She, Maoyun Yin, Guixiang Li, Jiarui Li, Xing Du, Lipu Ma, Wujun Ye, Xingguo |
| description | Regeneration of cultured tissue is a prerequisite of Agrobacterium- and biolistic-mediated plant transformation. In this study, an efficient protocol for improving wheat (Triticum aestivum L.) immature embryo regeneration was developed. Based on the statistical analysis of embryogenic callus induction efficiency, green spot differentiation efficiency, and plant regeneration efficiency from five wheat accessions, improved culture conditions were found to be more effective for embryogenic callus production than the traditional conditions. Using semi-quantitative reverse transcription polymerase chain reaction, a candidate gene, designated as TaCAT1, which encodes a catalase was identified to have a significant correlation with high-regeneration trait of wheat immature embryos. Three amino acid substitutions were found in TaCAT1 protein between high- and low-regeneration wheat accessions. Hydrogen peroxide content in the cultured calli increased from day 5 to 15, and then decreased sharply on day 20, followed by a second peak on day 25 during regeneration stage. Furthermore, a 3,500-bp 5′ flanking region upstream of the first codon ATG of TaCAT1 was isolated using inverse polymerase chain reaction. In silico, analysis revealed that the TaCAT1 promoter contained two regulatory motifs associated with responses to auxin. |
| doi_str_mv | 10.1007/s12033-012-9583-y |
| format | Article |
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In this study, an efficient protocol for improving wheat (Triticum aestivum L.) immature embryo regeneration was developed. Based on the statistical analysis of embryogenic callus induction efficiency, green spot differentiation efficiency, and plant regeneration efficiency from five wheat accessions, improved culture conditions were found to be more effective for embryogenic callus production than the traditional conditions. Using semi-quantitative reverse transcription polymerase chain reaction, a candidate gene, designated as TaCAT1, which encodes a catalase was identified to have a significant correlation with high-regeneration trait of wheat immature embryos. Three amino acid substitutions were found in TaCAT1 protein between high- and low-regeneration wheat accessions. Hydrogen peroxide content in the cultured calli increased from day 5 to 15, and then decreased sharply on day 20, followed by a second peak on day 25 during regeneration stage. Furthermore, a 3,500-bp 5′ flanking region upstream of the first codon ATG of TaCAT1 was isolated using inverse polymerase chain reaction. In silico, analysis revealed that the TaCAT1 promoter contained two regulatory motifs associated with responses to auxin.</description><identifier>ISSN: 1073-6085</identifier><identifier>EISSN: 1559-0305</identifier><identifier>DOI: 10.1007/s12033-012-9583-y</identifier><identifier>PMID: 22815184</identifier><language>eng</language><publisher>New York: Springer-Verlag</publisher><subject>Amino Acid Substitution ; Amino acids ; auxins ; Biochemistry ; Biological Techniques ; Biotechnology ; callus ; catalase ; Catalase - metabolism ; Cell Biology ; Chemistry ; Chemistry and Materials Science ; Crop science ; Embryonic growth stage ; Embryos ; Enzymes ; exposure duration ; genes ; Hormones ; Human Genetics ; Hydrogen peroxide ; Hydrogen Peroxide - metabolism ; Indoleacetic Acids - pharmacology ; Metabolism ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Somatic Embryogenesis Techniques - methods ; Promoter Regions, Genetic - genetics ; Protein Science ; Regeneration - genetics ; reverse transcriptase polymerase chain reaction ; Seeds - embryology ; Seeds - genetics ; Seeds - metabolism ; somatic embryogenesis ; Statistical analysis ; Tissue Culture Techniques - methods ; Triticum - embryology ; Triticum - genetics ; Triticum - metabolism ; Triticum aestivum ; Wheat</subject><ispartof>Molecular biotechnology, 2013-06, Vol.54 (2), p.451-460</ispartof><rights>Springer Science+Business Media, LLC 2012</rights><rights>Springer Science+Business Media New York 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c429t-935c6922f4c87037e27d27c9d0b2fb288706830be38bd768ceb0efa8f877e23c3</citedby><cites>FETCH-LOGICAL-c429t-935c6922f4c87037e27d27c9d0b2fb288706830be38bd768ceb0efa8f877e23c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12033-012-9583-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12033-012-9583-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22815184$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>She, Maoyun</creatorcontrib><creatorcontrib>Yin, Guixiang</creatorcontrib><creatorcontrib>Li, Jiarui</creatorcontrib><creatorcontrib>Li, Xing</creatorcontrib><creatorcontrib>Du, Lipu</creatorcontrib><creatorcontrib>Ma, Wujun</creatorcontrib><creatorcontrib>Ye, Xingguo</creatorcontrib><title>Efficient Regeneration Potential is Closely Related to Auxin Exposure Time and Catalase Metabolism During the Somatic Embryogenesis of Immature Embryos in Triticum aestivum L</title><title>Molecular biotechnology</title><addtitle>Mol Biotechnol</addtitle><addtitle>Mol Biotechnol</addtitle><description>Regeneration of cultured tissue is a prerequisite of Agrobacterium- and biolistic-mediated plant transformation. In this study, an efficient protocol for improving wheat (Triticum aestivum L.) immature embryo regeneration was developed. Based on the statistical analysis of embryogenic callus induction efficiency, green spot differentiation efficiency, and plant regeneration efficiency from five wheat accessions, improved culture conditions were found to be more effective for embryogenic callus production than the traditional conditions. Using semi-quantitative reverse transcription polymerase chain reaction, a candidate gene, designated as TaCAT1, which encodes a catalase was identified to have a significant correlation with high-regeneration trait of wheat immature embryos. Three amino acid substitutions were found in TaCAT1 protein between high- and low-regeneration wheat accessions. Hydrogen peroxide content in the cultured calli increased from day 5 to 15, and then decreased sharply on day 20, followed by a second peak on day 25 during regeneration stage. Furthermore, a 3,500-bp 5′ flanking region upstream of the first codon ATG of TaCAT1 was isolated using inverse polymerase chain reaction. In silico, analysis revealed that the TaCAT1 promoter contained two regulatory motifs associated with responses to auxin.</description><subject>Amino Acid Substitution</subject><subject>Amino acids</subject><subject>auxins</subject><subject>Biochemistry</subject><subject>Biological Techniques</subject><subject>Biotechnology</subject><subject>callus</subject><subject>catalase</subject><subject>Catalase - metabolism</subject><subject>Cell Biology</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Crop science</subject><subject>Embryonic growth stage</subject><subject>Embryos</subject><subject>Enzymes</subject><subject>exposure duration</subject><subject>genes</subject><subject>Hormones</subject><subject>Human Genetics</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Indoleacetic Acids - pharmacology</subject><subject>Metabolism</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Somatic Embryogenesis Techniques - methods</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Protein Science</subject><subject>Regeneration - genetics</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>Seeds - embryology</subject><subject>Seeds - genetics</subject><subject>Seeds - metabolism</subject><subject>somatic embryogenesis</subject><subject>Statistical analysis</subject><subject>Tissue Culture Techniques - methods</subject><subject>Triticum - embryology</subject><subject>Triticum - genetics</subject><subject>Triticum - metabolism</subject><subject>Triticum aestivum</subject><subject>Wheat</subject><issn>1073-6085</issn><issn>1559-0305</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kU1v1DAQhiMEoqXwA7iAJS5cAv7Ih32slgUqLQLR7dlynPHiKokX20HdP8VvZKIUhDhw8mjmmfe15i2K54y-YZS2bxPjVIiSMl6qWory9KA4Z3WtSipo_RBr2oqyobI-K56kdEspZ3UlHhdnnEtWM1mdFz-3znnrYcrkKxxggmiyDxP5EjL2vBmIT2QzhATDCYnBZOhJDuRyvvMT2d4dQ5ojkL0fgZipJxuTzWASkE-QTRcGn0bybo5-OpD8Dch1GFHfku3YxVNY_BLqB0euRhwsSuskEVTfR4_sPBIDKfsfWOyeFo-cGRI8u38vipv32_3mY7n7_OFqc7krbcVVLpWobaM4d5WVLRUt8LbnrVU97bjruMRmIwXtQMiubxtpoaPgjHSyRVZYcVG8XnWPMXyf0V6PPlkYBjNBmJNmosYbSiUUoq_-QW_DHCf8HVJVw1RVNQ1SbKVsDClFcPoY_WjiSTOqlzD1GqbGMPUSpj7hzot75bkbof-z8Ts9BPgKpONyYYh_Wf9H9eW65EzQ5hB90jfXnLKaUqpapSrxCwOYthY</recordid><startdate>20130601</startdate><enddate>20130601</enddate><creator>She, Maoyun</creator><creator>Yin, Guixiang</creator><creator>Li, Jiarui</creator><creator>Li, Xing</creator><creator>Du, Lipu</creator><creator>Ma, Wujun</creator><creator>Ye, Xingguo</creator><general>Springer-Verlag</general><general>Humana Press Inc</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope></search><sort><creationdate>20130601</creationdate><title>Efficient Regeneration Potential is Closely Related to Auxin Exposure Time and Catalase Metabolism During the Somatic Embryogenesis of Immature Embryos in Triticum aestivum L</title><author>She, Maoyun ; Yin, Guixiang ; Li, Jiarui ; Li, Xing ; Du, Lipu ; Ma, Wujun ; Ye, Xingguo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c429t-935c6922f4c87037e27d27c9d0b2fb288706830be38bd768ceb0efa8f877e23c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Amino Acid Substitution</topic><topic>Amino acids</topic><topic>auxins</topic><topic>Biochemistry</topic><topic>Biological Techniques</topic><topic>Biotechnology</topic><topic>callus</topic><topic>catalase</topic><topic>Catalase - 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methods</topic><topic>Triticum - embryology</topic><topic>Triticum - genetics</topic><topic>Triticum - metabolism</topic><topic>Triticum aestivum</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>She, Maoyun</creatorcontrib><creatorcontrib>Yin, Guixiang</creatorcontrib><creatorcontrib>Li, Jiarui</creatorcontrib><creatorcontrib>Li, Xing</creatorcontrib><creatorcontrib>Du, Lipu</creatorcontrib><creatorcontrib>Ma, Wujun</creatorcontrib><creatorcontrib>Ye, Xingguo</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><jtitle>Molecular biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>She, Maoyun</au><au>Yin, Guixiang</au><au>Li, Jiarui</au><au>Li, Xing</au><au>Du, Lipu</au><au>Ma, Wujun</au><au>Ye, Xingguo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efficient Regeneration Potential is Closely Related to Auxin Exposure Time and Catalase Metabolism During the Somatic Embryogenesis of Immature Embryos in Triticum aestivum L</atitle><jtitle>Molecular biotechnology</jtitle><stitle>Mol Biotechnol</stitle><addtitle>Mol Biotechnol</addtitle><date>2013-06-01</date><risdate>2013</risdate><volume>54</volume><issue>2</issue><spage>451</spage><epage>460</epage><pages>451-460</pages><issn>1073-6085</issn><eissn>1559-0305</eissn><abstract>Regeneration of cultured tissue is a prerequisite of Agrobacterium- and biolistic-mediated plant transformation. In this study, an efficient protocol for improving wheat (Triticum aestivum L.) immature embryo regeneration was developed. Based on the statistical analysis of embryogenic callus induction efficiency, green spot differentiation efficiency, and plant regeneration efficiency from five wheat accessions, improved culture conditions were found to be more effective for embryogenic callus production than the traditional conditions. Using semi-quantitative reverse transcription polymerase chain reaction, a candidate gene, designated as TaCAT1, which encodes a catalase was identified to have a significant correlation with high-regeneration trait of wheat immature embryos. Three amino acid substitutions were found in TaCAT1 protein between high- and low-regeneration wheat accessions. Hydrogen peroxide content in the cultured calli increased from day 5 to 15, and then decreased sharply on day 20, followed by a second peak on day 25 during regeneration stage. Furthermore, a 3,500-bp 5′ flanking region upstream of the first codon ATG of TaCAT1 was isolated using inverse polymerase chain reaction. In silico, analysis revealed that the TaCAT1 promoter contained two regulatory motifs associated with responses to auxin.</abstract><cop>New York</cop><pub>Springer-Verlag</pub><pmid>22815184</pmid><doi>10.1007/s12033-012-9583-y</doi><tpages>10</tpages></addata></record> |
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| subjects | Amino Acid Substitution Amino acids auxins Biochemistry Biological Techniques Biotechnology callus catalase Catalase - metabolism Cell Biology Chemistry Chemistry and Materials Science Crop science Embryonic growth stage Embryos Enzymes exposure duration genes Hormones Human Genetics Hydrogen peroxide Hydrogen Peroxide - metabolism Indoleacetic Acids - pharmacology Metabolism Plant Proteins - genetics Plant Proteins - metabolism Plant Somatic Embryogenesis Techniques - methods Promoter Regions, Genetic - genetics Protein Science Regeneration - genetics reverse transcriptase polymerase chain reaction Seeds - embryology Seeds - genetics Seeds - metabolism somatic embryogenesis Statistical analysis Tissue Culture Techniques - methods Triticum - embryology Triticum - genetics Triticum - metabolism Triticum aestivum Wheat |
| title | Efficient Regeneration Potential is Closely Related to Auxin Exposure Time and Catalase Metabolism During the Somatic Embryogenesis of Immature Embryos in Triticum aestivum L |
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