“Salt tolerant” anion exchange chromatography for direct capture of an acidic protein from CHO cell culture

•HyperCel STAR AX is a salt tolerant sorbent.•HyperCel STAR AX efficiently purifies active enzyme from neat feedstock.•Higher capacity of HyperCel STAR AX compared to standard AEX with neat feedstock.•Same optimization of operating conditions for HyperCel STAR AX as for standard AEX.•Flexibility of...

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Veröffentlicht in:	Protein expression and purification 2013-06, Vol.89 (2), p.117-123
Hauptverfasser:	Champagne, Jérôme, Balluet, Guillaume, Gantier, René, Toueille, Magali
Format:	Artikel
Sprache:	eng
Schlagworte:	Adsorption alpha-Amylases - chemistry alpha-Amylases - isolation & purification alpha-Amylases - metabolism Animals Anion exchange chromatography Anions - chemistry CHO Cells CHO proteins Chromatography, Ion Exchange - methods Cricetinae Cricetulus Enzyme Stability Flexibility Productivity Salt tolerance Salts - chemistry α-Amylase
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