Toxoplasma gondii tachyzoite-infected peripheral blood mononuclear cells are enriched in mouse lungs and liver

[Display omitted] •We developed a method to estimate tachyzoite and PBMC numbers in solid organs.•The PBMC infection rates in several mouse organs were compared.•The tachyzoite-infected PBMCs were enriched in mouse lungs and liver. The intracellular parasite Toxoplasma gondii is thought to dissemina...

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Veröffentlicht in:	Experimental parasitology 2013-06, Vol.134 (2), p.160-164
Hauptverfasser:	Unno, Akihiro, Kachi, Seira, Batanova, Tatiana A., Ohno, Tamio, Elhawary, Nagwa, Kitoh, Katsuya, Takashima, Yasuhiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals brain cell movement Cercopithecus aethiops Circulation DNA, Protozoan - isolation & purification fluorescence genetically modified organisms Green Fluorescent Proteins Heart Ventricles - parasitology Heart Ventricles - pathology inoculum Leukocytes, Mononuclear - cytology Leukocytes, Mononuclear - parasitology liver Liver - parasitology Liver - pathology Luminescent Agents Lung - parasitology Lung - pathology lungs Male Mice Mice, Inbred C57BL mononuclear leukocytes parasites parasitology Peripheral blood mononuclear cells (PBMCs) quantitative polymerase chain reaction Real-Time Polymerase Chain Reaction Real-time quantitative PCR (qRT-PCR) reverse transcriptase polymerase chain reaction spleen Spleen - parasitology Spleen - pathology Tachyzoite tail tissues Toxoplasma - genetics Toxoplasma - isolation & purification Toxoplasma - physiology Toxoplasma gondii Toxoplasmosis, Animal - blood Toxoplasmosis, Animal - parasitology Toxoplasmosis, Animal - pathology Vero Cells
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