Optimization of single-tube nested PCR for the diagnosis of visceral leishmaniasis

•We proposed to optimize a single-tube nested PCR (STNPCR) method.•We compared the performance of STNPCR and nested PCR for detecting Leishmania chagasi DNA.•The detection limit for L. chagasi DNA was 1fg to nested PCR and 10fg to STNPCR.•STNPCR is a promising tool for diagnosing visceral leishmania...

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Veröffentlicht in:	Experimental parasitology 2013-06, Vol.134 (2), p.206-210
Hauptverfasser:	da Silva, Maria Almerice Lopes, Pedrosa Soares, Cynthia Regina, Medeiros, Rafael Acioli, Medeiros, Zulma, de Melo, Fábio Lopes
Format:	Artikel
Sprache:	eng
Schlagworte:	adsorption Chagas disease cross contamination detection limit Diagnosing DNA DNA, Protozoan - analysis DNA, Ribosomal - analysis DNA, Ribosomal Spacer - genetics internal transcribed spacers Leishmania Leishmania braziliensis - genetics Leishmania braziliensis - isolation & purification Leishmania infantum - genetics Leishmania infantum - isolation & purification Leishmania major - genetics Leishmania major - isolation & purification Leishmania mexicana - genetics Leishmania mexicana - isolation & purification Leishmaniasis, Visceral - diagnosis mixed infection parasites parasitology PCR polymerase chain reaction Polymerase Chain Reaction - methods Polymerase Chain Reaction - standards ribosomal RNA risk RNA, Ribosomal - genetics Sensitivity and Specificity solubilization Species Specificity Trypanosoma cruzi Trypanosoma cruzi - genetics Trypanosoma cruzi - isolation & purification Visceral leishmaniasis
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