Tetracycline Resistance and Class 1 Integron Genes Associated with Indoor and Outdoor Aerosols
Genes encoding tetracycline resistance and the integrase of Class 1 integrons were enumerated using quantitative PCR from aerosols collected from indoor and outdoor environments. Concentrated animal feeding operations (CAFOs) and human-occupied indoor environments (two clinics and a homeless shelter...
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| Veröffentlicht in: | Environmental science & technology 2013-05, Vol.47 (9), p.4046-4052 |
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| creator | Ling, Alison L Pace, Norman R Hernandez, Mark T LaPara, Timothy M |
| description | Genes encoding tetracycline resistance and the integrase of Class 1 integrons were enumerated using quantitative PCR from aerosols collected from indoor and outdoor environments. Concentrated animal feeding operations (CAFOs) and human-occupied indoor environments (two clinics and a homeless shelter) were found to be a source of airborne tet(X) and tet(W) genes. The CAFOs had 10- to 100-times higher concentrations of airborne 16S rRNA, tet(X), and tet(W) genes than other environments sampled, and increased concentrations of aerosolized bacteria correlated with increased concentrations of airborne resistance genes. The two CAFOs studied had statistically similar concentrations of resistance genes in their aerosol samples, even though antibiotic use was markedly different between the two operations. Additionally, tet(W) genes were recovered in outdoor air within 2 km of livestock operations, which suggests that antibiotic resistance genes may be transported via aerosols on local scales. The integrase gene (intI1) from Class 1 integrons, which has been associated with multidrug resistance, was detected in CAFOs but not in human-occupied indoor environments, suggesting that CAFO aerosols could serve as a reservoir of multidrug resistance. In conclusion, our results show that CAFOs and clinics are sources of aerosolized antibiotic resistance genes that can potentially be transported via air movement. |
| doi_str_mv | 10.1021/es400238g |
| format | Article |
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Concentrated animal feeding operations (CAFOs) and human-occupied indoor environments (two clinics and a homeless shelter) were found to be a source of airborne tet(X) and tet(W) genes. The CAFOs had 10- to 100-times higher concentrations of airborne 16S rRNA, tet(X), and tet(W) genes than other environments sampled, and increased concentrations of aerosolized bacteria correlated with increased concentrations of airborne resistance genes. The two CAFOs studied had statistically similar concentrations of resistance genes in their aerosol samples, even though antibiotic use was markedly different between the two operations. Additionally, tet(W) genes were recovered in outdoor air within 2 km of livestock operations, which suggests that antibiotic resistance genes may be transported via aerosols on local scales. The integrase gene (intI1) from Class 1 integrons, which has been associated with multidrug resistance, was detected in CAFOs but not in human-occupied indoor environments, suggesting that CAFO aerosols could serve as a reservoir of multidrug resistance. In conclusion, our results show that CAFOs and clinics are sources of aerosolized antibiotic resistance genes that can potentially be transported via air movement.</description><identifier>ISSN: 0013-936X</identifier><identifier>EISSN: 1520-5851</identifier><identifier>DOI: 10.1021/es400238g</identifier><identifier>PMID: 23517146</identifier><identifier>CODEN: ESTHAG</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Aerosols ; Air Microbiology ; Animal, plant and microbial ecology ; Animals ; Antibiotics ; Applied ecology ; Biological and medical sciences ; Correlation analysis ; Deoxyribonucleic acid ; DNA ; Drug resistance ; Ecotoxicology, biological effects of pollution ; Fundamental and applied biological sciences. 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Sci. Technol</addtitle><description>Genes encoding tetracycline resistance and the integrase of Class 1 integrons were enumerated using quantitative PCR from aerosols collected from indoor and outdoor environments. Concentrated animal feeding operations (CAFOs) and human-occupied indoor environments (two clinics and a homeless shelter) were found to be a source of airborne tet(X) and tet(W) genes. The CAFOs had 10- to 100-times higher concentrations of airborne 16S rRNA, tet(X), and tet(W) genes than other environments sampled, and increased concentrations of aerosolized bacteria correlated with increased concentrations of airborne resistance genes. The two CAFOs studied had statistically similar concentrations of resistance genes in their aerosol samples, even though antibiotic use was markedly different between the two operations. Additionally, tet(W) genes were recovered in outdoor air within 2 km of livestock operations, which suggests that antibiotic resistance genes may be transported via aerosols on local scales. The integrase gene (intI1) from Class 1 integrons, which has been associated with multidrug resistance, was detected in CAFOs but not in human-occupied indoor environments, suggesting that CAFO aerosols could serve as a reservoir of multidrug resistance. In conclusion, our results show that CAFOs and clinics are sources of aerosolized antibiotic resistance genes that can potentially be transported via air movement.</description><subject>Aerosols</subject><subject>Air Microbiology</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Antibiotics</subject><subject>Applied ecology</subject><subject>Biological and medical sciences</subject><subject>Correlation analysis</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Drug resistance</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Genetics</subject><subject>Homeless people</subject><subject>Integrons - genetics</subject><subject>Polymerase Chain Reaction</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Tetracycline Resistance - genetics</subject><issn>0013-936X</issn><issn>1520-5851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpl0F1LwzAUBuAgipsfF_4BKYigF9WkSdrscgydwkAQBa8saXoyO7pGc1pk_97sQyd6lUCenLx5CTlh9IrRhF0DCkoTrqY7pM9kQmOpJNslfUoZjwc8femRA8QZXSKq9kkv4ZJlTKR98voErddmYeqqgegRsMJWNwYi3ZTRqNaIEYvumxam3jXRGBrAaIjoTKVbKKPPqn0Lx6VzfnXjoWtX-yF4h67GI7JndY1wvFkPyfPtzdPoLp48jO9Hw0msecbbWDKV2URSznXGTJoKUQCjQqa2KAxPuaI2KwWY0oI1SSalKrQVqaRK8YInlB-Si_Xcd-8-OsA2n1dooK51A67DnHExEJRJNQj07A-duc43Id1KqRBoIIK6XCsTPoIebP7uq7n2i5zRfFl6_lN6sKebiV0xh_JHfrccwPkGaDS6tj40XOHWZVwKydnWaYO_Uv178AtTkZOq</recordid><startdate>20130507</startdate><enddate>20130507</enddate><creator>Ling, Alison L</creator><creator>Pace, Norman R</creator><creator>Hernandez, Mark T</creator><creator>LaPara, Timothy M</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>20130507</creationdate><title>Tetracycline Resistance and Class 1 Integron Genes Associated with Indoor and Outdoor Aerosols</title><author>Ling, Alison L ; Pace, Norman R ; Hernandez, Mark T ; LaPara, Timothy M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a373t-5187f25033a71c6644be10456fbbc36380f7d4ecdfefc27558baf4650883b3203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Aerosols</topic><topic>Air Microbiology</topic><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Antibiotics</topic><topic>Applied ecology</topic><topic>Biological and medical sciences</topic><topic>Correlation analysis</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Drug resistance</topic><topic>Ecotoxicology, biological effects of pollution</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Genetics</topic><topic>Homeless people</topic><topic>Integrons - genetics</topic><topic>Polymerase Chain Reaction</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Tetracycline Resistance - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ling, Alison L</creatorcontrib><creatorcontrib>Pace, Norman R</creatorcontrib><creatorcontrib>Hernandez, Mark T</creatorcontrib><creatorcontrib>LaPara, Timothy M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Environmental science & technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ling, Alison L</au><au>Pace, Norman R</au><au>Hernandez, Mark T</au><au>LaPara, Timothy M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tetracycline Resistance and Class 1 Integron Genes Associated with Indoor and Outdoor Aerosols</atitle><jtitle>Environmental science & technology</jtitle><addtitle>Environ. Sci. Technol</addtitle><date>2013-05-07</date><risdate>2013</risdate><volume>47</volume><issue>9</issue><spage>4046</spage><epage>4052</epage><pages>4046-4052</pages><issn>0013-936X</issn><eissn>1520-5851</eissn><coden>ESTHAG</coden><abstract>Genes encoding tetracycline resistance and the integrase of Class 1 integrons were enumerated using quantitative PCR from aerosols collected from indoor and outdoor environments. Concentrated animal feeding operations (CAFOs) and human-occupied indoor environments (two clinics and a homeless shelter) were found to be a source of airborne tet(X) and tet(W) genes. The CAFOs had 10- to 100-times higher concentrations of airborne 16S rRNA, tet(X), and tet(W) genes than other environments sampled, and increased concentrations of aerosolized bacteria correlated with increased concentrations of airborne resistance genes. The two CAFOs studied had statistically similar concentrations of resistance genes in their aerosol samples, even though antibiotic use was markedly different between the two operations. Additionally, tet(W) genes were recovered in outdoor air within 2 km of livestock operations, which suggests that antibiotic resistance genes may be transported via aerosols on local scales. The integrase gene (intI1) from Class 1 integrons, which has been associated with multidrug resistance, was detected in CAFOs but not in human-occupied indoor environments, suggesting that CAFO aerosols could serve as a reservoir of multidrug resistance. In conclusion, our results show that CAFOs and clinics are sources of aerosolized antibiotic resistance genes that can potentially be transported via air movement.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>23517146</pmid><doi>10.1021/es400238g</doi><tpages>7</tpages></addata></record> |
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| subjects | Aerosols Air Microbiology Animal, plant and microbial ecology Animals Antibiotics Applied ecology Biological and medical sciences Correlation analysis Deoxyribonucleic acid DNA Drug resistance Ecotoxicology, biological effects of pollution Fundamental and applied biological sciences. Psychology General aspects Genetics Homeless people Integrons - genetics Polymerase Chain Reaction RNA, Ribosomal, 16S - genetics Tetracycline Resistance - genetics |
| title | Tetracycline Resistance and Class 1 Integron Genes Associated with Indoor and Outdoor Aerosols |
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