Dry powder preparation of inulin fructotransferase from Arthrobacter aurescens SK 8.001 fermented liquor
► IFTase powder preparation from fermented liquor was founded. ► IFTase powder (10.2g) was obtained and specific activity was 16.4U/mg. ► The method is easy to scale up DFA III preparation. Difructosan anhydrides III (DFA III) are usually obtained by inulin conversion with inulin fructotransferase (...
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| Veröffentlicht in: | Carbohydrate polymers 2013-06, Vol.95 (2), p.654-656 |
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| container_title | Carbohydrate polymers |
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| creator | Hang, Hua Li, Yungao Zhao, Meng Jiang, Bo Miao, Ming Mu, Wanmeng Zhang, Tao |
| description | ► IFTase powder preparation from fermented liquor was founded. ► IFTase powder (10.2g) was obtained and specific activity was 16.4U/mg. ► The method is easy to scale up DFA III preparation.
Difructosan anhydrides III (DFA III) are usually obtained by inulin conversion with inulin fructotransferase (IFTase). IFTase liquor is difficult to store for a long time, which could greatly restrict its application and DFA III production. To meet DFA III scale-up preparation, this work was explored to research dry powder preparation of IFTase from Arthrobacter aurescens SK 8.001 fermented liquor by ultrafiltration concentration, ammonium sulfate precipitation and freeze drying. IFTase powder (10.2g) was obtained from IFTase precipitation (126.4g) and its specific activity determined was 16.4U/mg. Dry powder of IFTase could maintain over 120days at different temperatures. These results showed that it is easy to scale up DFA III preparation for industrial capacity. |
| doi_str_mv | 10.1016/j.carbpol.2013.02.058 |
| format | Article |
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Difructosan anhydrides III (DFA III) are usually obtained by inulin conversion with inulin fructotransferase (IFTase). IFTase liquor is difficult to store for a long time, which could greatly restrict its application and DFA III production. To meet DFA III scale-up preparation, this work was explored to research dry powder preparation of IFTase from Arthrobacter aurescens SK 8.001 fermented liquor by ultrafiltration concentration, ammonium sulfate precipitation and freeze drying. IFTase powder (10.2g) was obtained from IFTase precipitation (126.4g) and its specific activity determined was 16.4U/mg. Dry powder of IFTase could maintain over 120days at different temperatures. These results showed that it is easy to scale up DFA III preparation for industrial capacity.</description><identifier>ISSN: 0144-8617</identifier><identifier>EISSN: 1879-1344</identifier><identifier>DOI: 10.1016/j.carbpol.2013.02.058</identifier><identifier>PMID: 23648026</identifier><identifier>CODEN: CAPOD8</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>ammonium sulfate ; Ammonium sulfate precipitation ; anhydrides ; Arthrobacter - chemistry ; Arthrobacter - enzymology ; Arthrobacter aurescens ; Biological and medical sciences ; Biotechnology ; DFA III ; Enzyme engineering ; Fermentation ; Freeze Drying ; Fundamental and applied biological sciences. Psychology ; Hexosyltransferases - chemistry ; Hexosyltransferases - isolation & purification ; IFTase ; inulin ; Methods. Procedures. Technologies ; Powders - chemistry ; Production of selected enzymes ; temperature ; Ultrafiltration</subject><ispartof>Carbohydrate polymers, 2013-06, Vol.95 (2), p.654-656</ispartof><rights>2013 Elsevier Ltd</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-66c4de20bd90f5d874f02dcc438f1d7cf7d445c91fe985bca548f5865d43146a3</citedby><cites>FETCH-LOGICAL-c419t-66c4de20bd90f5d874f02dcc438f1d7cf7d445c91fe985bca548f5865d43146a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.carbpol.2013.02.058$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27382826$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23648026$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hang, Hua</creatorcontrib><creatorcontrib>Li, Yungao</creatorcontrib><creatorcontrib>Zhao, Meng</creatorcontrib><creatorcontrib>Jiang, Bo</creatorcontrib><creatorcontrib>Miao, Ming</creatorcontrib><creatorcontrib>Mu, Wanmeng</creatorcontrib><creatorcontrib>Zhang, Tao</creatorcontrib><title>Dry powder preparation of inulin fructotransferase from Arthrobacter aurescens SK 8.001 fermented liquor</title><title>Carbohydrate polymers</title><addtitle>Carbohydr Polym</addtitle><description>► IFTase powder preparation from fermented liquor was founded. ► IFTase powder (10.2g) was obtained and specific activity was 16.4U/mg. ► The method is easy to scale up DFA III preparation.
Difructosan anhydrides III (DFA III) are usually obtained by inulin conversion with inulin fructotransferase (IFTase). IFTase liquor is difficult to store for a long time, which could greatly restrict its application and DFA III production. To meet DFA III scale-up preparation, this work was explored to research dry powder preparation of IFTase from Arthrobacter aurescens SK 8.001 fermented liquor by ultrafiltration concentration, ammonium sulfate precipitation and freeze drying. IFTase powder (10.2g) was obtained from IFTase precipitation (126.4g) and its specific activity determined was 16.4U/mg. Dry powder of IFTase could maintain over 120days at different temperatures. These results showed that it is easy to scale up DFA III preparation for industrial capacity.</description><subject>ammonium sulfate</subject><subject>Ammonium sulfate precipitation</subject><subject>anhydrides</subject><subject>Arthrobacter - chemistry</subject><subject>Arthrobacter - enzymology</subject><subject>Arthrobacter aurescens</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>DFA III</subject><subject>Enzyme engineering</subject><subject>Fermentation</subject><subject>Freeze Drying</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hexosyltransferases - chemistry</subject><subject>Hexosyltransferases - isolation & purification</subject><subject>IFTase</subject><subject>inulin</subject><subject>Methods. Procedures. Technologies</subject><subject>Powders - chemistry</subject><subject>Production of selected enzymes</subject><subject>temperature</subject><subject>Ultrafiltration</subject><issn>0144-8617</issn><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1TAQRi0EoreFRwCyQeomwU5sx16hqvyKSixK15Zjj6mvkjgdJ6C-PS73Aku8Gck638zoDCEvGG0YZfLNvnEWhyWNTUtZ19C2oUI9Ijumel2zjvPHZEcZ57WSrD8hpznvaXmS0afkpO0kV7SVO3L7Du-rJf30gNWCsFi0a0xzlUIV522McxVwc2ta0c45ANoM5SdN1QWut5gG69aStBtCdjDn6vpLpRpKWVXYCeYVfDXGuy3hM_Ik2DHD82M9Izcf3n-7_FRfff34-fLiqnac6bWW0nEPLR28pkF41fNAW-8c71Rgvneh95wLp1kArcTgrOAqCCWF5x3j0nZn5PzQd8F0t0FezRTLauNoZ0hbNkWN7rTWghZUHFCHKWeEYBaMk8V7w6h5kGz25ijZPEg2tDVFcsm9PI7Yhgn839QfqwV4fQRsdnYMxZ2L-R_Xd6pVv7lXBy7YZOx3LMzNdZkky6FER7UuxNsDAUXZjwhososwO_ARwa3Gp_ifZX8BeQunUw</recordid><startdate>20130620</startdate><enddate>20130620</enddate><creator>Hang, Hua</creator><creator>Li, Yungao</creator><creator>Zhao, Meng</creator><creator>Jiang, Bo</creator><creator>Miao, Ming</creator><creator>Mu, Wanmeng</creator><creator>Zhang, Tao</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130620</creationdate><title>Dry powder preparation of inulin fructotransferase from Arthrobacter aurescens SK 8.001 fermented liquor</title><author>Hang, Hua ; Li, Yungao ; Zhao, Meng ; Jiang, Bo ; Miao, Ming ; Mu, Wanmeng ; Zhang, Tao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-66c4de20bd90f5d874f02dcc438f1d7cf7d445c91fe985bca548f5865d43146a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>ammonium sulfate</topic><topic>Ammonium sulfate precipitation</topic><topic>anhydrides</topic><topic>Arthrobacter - chemistry</topic><topic>Arthrobacter - enzymology</topic><topic>Arthrobacter aurescens</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>DFA III</topic><topic>Enzyme engineering</topic><topic>Fermentation</topic><topic>Freeze Drying</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hexosyltransferases - chemistry</topic><topic>Hexosyltransferases - isolation & purification</topic><topic>IFTase</topic><topic>inulin</topic><topic>Methods. Procedures. Technologies</topic><topic>Powders - chemistry</topic><topic>Production of selected enzymes</topic><topic>temperature</topic><topic>Ultrafiltration</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hang, Hua</creatorcontrib><creatorcontrib>Li, Yungao</creatorcontrib><creatorcontrib>Zhao, Meng</creatorcontrib><creatorcontrib>Jiang, Bo</creatorcontrib><creatorcontrib>Miao, Ming</creatorcontrib><creatorcontrib>Mu, Wanmeng</creatorcontrib><creatorcontrib>Zhang, Tao</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hang, Hua</au><au>Li, Yungao</au><au>Zhao, Meng</au><au>Jiang, Bo</au><au>Miao, Ming</au><au>Mu, Wanmeng</au><au>Zhang, Tao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dry powder preparation of inulin fructotransferase from Arthrobacter aurescens SK 8.001 fermented liquor</atitle><jtitle>Carbohydrate polymers</jtitle><addtitle>Carbohydr Polym</addtitle><date>2013-06-20</date><risdate>2013</risdate><volume>95</volume><issue>2</issue><spage>654</spage><epage>656</epage><pages>654-656</pages><issn>0144-8617</issn><eissn>1879-1344</eissn><coden>CAPOD8</coden><abstract>► IFTase powder preparation from fermented liquor was founded. ► IFTase powder (10.2g) was obtained and specific activity was 16.4U/mg. ► The method is easy to scale up DFA III preparation.
Difructosan anhydrides III (DFA III) are usually obtained by inulin conversion with inulin fructotransferase (IFTase). IFTase liquor is difficult to store for a long time, which could greatly restrict its application and DFA III production. To meet DFA III scale-up preparation, this work was explored to research dry powder preparation of IFTase from Arthrobacter aurescens SK 8.001 fermented liquor by ultrafiltration concentration, ammonium sulfate precipitation and freeze drying. IFTase powder (10.2g) was obtained from IFTase precipitation (126.4g) and its specific activity determined was 16.4U/mg. Dry powder of IFTase could maintain over 120days at different temperatures. These results showed that it is easy to scale up DFA III preparation for industrial capacity.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>23648026</pmid><doi>10.1016/j.carbpol.2013.02.058</doi><tpages>3</tpages></addata></record> |
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| subjects | ammonium sulfate Ammonium sulfate precipitation anhydrides Arthrobacter - chemistry Arthrobacter - enzymology Arthrobacter aurescens Biological and medical sciences Biotechnology DFA III Enzyme engineering Fermentation Freeze Drying Fundamental and applied biological sciences. Psychology Hexosyltransferases - chemistry Hexosyltransferases - isolation & purification IFTase inulin Methods. Procedures. Technologies Powders - chemistry Production of selected enzymes temperature Ultrafiltration |
| title | Dry powder preparation of inulin fructotransferase from Arthrobacter aurescens SK 8.001 fermented liquor |
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