Modified MuDPIT Separation Identified 4488 Proteins in a System-wide Analysis of Quiescence in Yeast

A modified multidimensional protein identification technology (MudPIT) separation was coupled to an LTQ Orbitrap Velos mass spectrometer and used to rapidly identify the near-complete yeast proteome from a whole cell tryptic digest. This modified online two-dimensional liquid chromatography separati...

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Veröffentlicht in:	Journal of proteome research 2013-05, Vol.12 (5), p.2177-2184
Hauptverfasser:	Webb, Kristofor J, Xu, Tao, Park, Sung Kyu, Yates, John R
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Cycle Checkpoints Chromatography, High Pressure Liquid Chromatography, Ion Exchange Proteome - isolation & purification Proteome - metabolism Proteomics Saccharomyces cerevisiae - cytology Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - isolation & purification Saccharomyces cerevisiae Proteins - metabolism Tandem Mass Spectrometry
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