Antimicrobial efficacy of a human β-defensin-3 peptide using an Enterococcus faecalis dentine infection model
Aim To assess the antibacterial efficacy of a human β‐defensin‐3 (HBD3) peptide against Enterococcus faecalis biofilms. Methodology Standardized human dentine blocks were infected with E. faecalis ATCC 29212 for 3 weeks. Aqueous calcium hydroxide paste (n = 12, CH), a 2% chlorhexidine gel (n = 12, C...
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| Veröffentlicht in: | International endodontic journal 2013-05, Vol.46 (5), p.406-412 |
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| container_title | International endodontic journal |
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| creator | Lee, J.-K. Park, Y.-J. Kum, K.-Y. Han, S. H. Chang, S.-W. Kaufman, B. Jiang, J. Zhu, Q. Safavi, K. Spångberg, L. |
| description | Aim
To assess the antibacterial efficacy of a human β‐defensin‐3 (HBD3) peptide against Enterococcus faecalis biofilms.
Methodology
Standardized human dentine blocks were infected with E. faecalis ATCC 29212 for 3 weeks. Aqueous calcium hydroxide paste (n = 12, CH), a 2% chlorhexidine gel (n = 12, CHX), an HBD3 peptide gel (n = 12) and saline (n = 12) were tested as experimental groups. A mismatched peptide gel group (n = 12, MP) and sterilized but noninoculated block group (n = 12) were included as controls. After 1 week of medication, the dentinal samples at the depth of 200 and 400 μm were collected from medicated canal lumens. Bacterial growth was assessed by spectrophotometric analysis of optical density (OD) after 72 h of incubation. Statistical analysis was performed with repeated‐measures anova and Tukey's post hoc test.
Results
The HBD3 group was associated with significantly lower OD values (P 0.05). There was no significant difference (P > 0.05) in the OD values of the inner (200 μm) and outer (400 μm) dentinal samples for any group.
Conclusions
The HBD3 peptide inhibited the growth of E. faecalis biofilms in infected dentine blocks. |
| doi_str_mv | 10.1111/iej.12002 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_1348490409</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1326732290</sourcerecordid><originalsourceid>FETCH-LOGICAL-i2372-c42532f60dfa91b11ca8ecc26be75c7249855b1bdc836bf07dac783e9536809e3</originalsourceid><addsrcrecordid>eNqNkc9OwzAMxiMEgjE48AIoRy6F_Gna9AhoGyA0hABxjNLUgUCbjqYV7LV4EJ6JwIAzvtiWf58t-UNoj5JDGuPIwdMhZYSwNTSiPBMJEwVdRyNCU54wKcUW2g7hiRAiCKebaItxkksqshHyx753jTNdWzpdY7DWGW2WuLVY48eh0R5_vCcVWPDB-YTjBSx6VwEeYvuA43jie-ha0xozBGw1GF27gCuIez1g5y2Y3rUeN20F9Q7asLoOsPuTx-huOrk9PUsur2bnp8eXiWM8Z4lJmeDMZqSyuqAlpUZLMIZlJeTC5CwtpBAlLSsjeVZaklfa5JJDIXgmSQF8jA5Wexdd-zJA6FXjgoG61h7aISjKU5kWJCXFP1CW5ZyxgkR0_wcdygYqtehco7ul-n1nBI5WwKurYfk3p0R9-aSiT-rbJ3U-ufguoiJZKVzo4e1PobtnFa_mQt3PZyo9ubm-n87mivNPqIeUmQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1326732290</pqid></control><display><type>article</type><title>Antimicrobial efficacy of a human β-defensin-3 peptide using an Enterococcus faecalis dentine infection model</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Lee, J.-K. ; Park, Y.-J. ; Kum, K.-Y. ; Han, S. H. ; Chang, S.-W. ; Kaufman, B. ; Jiang, J. ; Zhu, Q. ; Safavi, K. ; Spångberg, L.</creator><creatorcontrib>Lee, J.-K. ; Park, Y.-J. ; Kum, K.-Y. ; Han, S. H. ; Chang, S.-W. ; Kaufman, B. ; Jiang, J. ; Zhu, Q. ; Safavi, K. ; Spångberg, L.</creatorcontrib><description>Aim
To assess the antibacterial efficacy of a human β‐defensin‐3 (HBD3) peptide against Enterococcus faecalis biofilms.
Methodology
Standardized human dentine blocks were infected with E. faecalis ATCC 29212 for 3 weeks. Aqueous calcium hydroxide paste (n = 12, CH), a 2% chlorhexidine gel (n = 12, CHX), an HBD3 peptide gel (n = 12) and saline (n = 12) were tested as experimental groups. A mismatched peptide gel group (n = 12, MP) and sterilized but noninoculated block group (n = 12) were included as controls. After 1 week of medication, the dentinal samples at the depth of 200 and 400 μm were collected from medicated canal lumens. Bacterial growth was assessed by spectrophotometric analysis of optical density (OD) after 72 h of incubation. Statistical analysis was performed with repeated‐measures anova and Tukey's post hoc test.
Results
The HBD3 group was associated with significantly lower OD values (P < 0.05) than the CH or CHX groups at both depths. The CH group did not differ significantly from MP or Saline group at either depth (P > 0.05). There was no significant difference (P > 0.05) in the OD values of the inner (200 μm) and outer (400 μm) dentinal samples for any group.
Conclusions
The HBD3 peptide inhibited the growth of E. faecalis biofilms in infected dentine blocks.</description><identifier>ISSN: 0143-2885</identifier><identifier>EISSN: 1365-2591</identifier><identifier>DOI: 10.1111/iej.12002</identifier><identifier>PMID: 23078156</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Anti-Infective Agents - therapeutic use ; Anti-Infective Agents, Local - therapeutic use ; Antimicrobial agents ; antimicrobial efficacy ; beta-Defensins - therapeutic use ; Biofilms - drug effects ; Calcium Hydroxide - therapeutic use ; Chlorhexidine - therapeutic use ; Dental Pulp Cavity - drug effects ; Dental Pulp Cavity - microbiology ; Dentin - drug effects ; Dentin - microbiology ; dentine block ; Dentistry ; E. faecalis biofilms ; Enterococcus faecalis ; Enterococcus faecalis - drug effects ; Gram-Positive Bacterial Infections - drug therapy ; human β-defensin-3 ; Humans ; Humidity ; Materials Testing ; Nephelometry and Turbidimetry ; optical density ; Root Canal Irrigants - therapeutic use ; Sodium Chloride ; Spectrophotometry ; Temperature ; Time Factors</subject><ispartof>International endodontic journal, 2013-05, Vol.46 (5), p.406-412</ispartof><rights>2012 International Endodontic Journal</rights><rights>2012 International Endodontic Journal.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fiej.12002$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fiej.12002$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23078156$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, J.-K.</creatorcontrib><creatorcontrib>Park, Y.-J.</creatorcontrib><creatorcontrib>Kum, K.-Y.</creatorcontrib><creatorcontrib>Han, S. H.</creatorcontrib><creatorcontrib>Chang, S.-W.</creatorcontrib><creatorcontrib>Kaufman, B.</creatorcontrib><creatorcontrib>Jiang, J.</creatorcontrib><creatorcontrib>Zhu, Q.</creatorcontrib><creatorcontrib>Safavi, K.</creatorcontrib><creatorcontrib>Spångberg, L.</creatorcontrib><title>Antimicrobial efficacy of a human β-defensin-3 peptide using an Enterococcus faecalis dentine infection model</title><title>International endodontic journal</title><addtitle>Int Endod J</addtitle><description>Aim
To assess the antibacterial efficacy of a human β‐defensin‐3 (HBD3) peptide against Enterococcus faecalis biofilms.
Methodology
Standardized human dentine blocks were infected with E. faecalis ATCC 29212 for 3 weeks. Aqueous calcium hydroxide paste (n = 12, CH), a 2% chlorhexidine gel (n = 12, CHX), an HBD3 peptide gel (n = 12) and saline (n = 12) were tested as experimental groups. A mismatched peptide gel group (n = 12, MP) and sterilized but noninoculated block group (n = 12) were included as controls. After 1 week of medication, the dentinal samples at the depth of 200 and 400 μm were collected from medicated canal lumens. Bacterial growth was assessed by spectrophotometric analysis of optical density (OD) after 72 h of incubation. Statistical analysis was performed with repeated‐measures anova and Tukey's post hoc test.
Results
The HBD3 group was associated with significantly lower OD values (P < 0.05) than the CH or CHX groups at both depths. The CH group did not differ significantly from MP or Saline group at either depth (P > 0.05). There was no significant difference (P > 0.05) in the OD values of the inner (200 μm) and outer (400 μm) dentinal samples for any group.
Conclusions
The HBD3 peptide inhibited the growth of E. faecalis biofilms in infected dentine blocks.</description><subject>Anti-Infective Agents - therapeutic use</subject><subject>Anti-Infective Agents, Local - therapeutic use</subject><subject>Antimicrobial agents</subject><subject>antimicrobial efficacy</subject><subject>beta-Defensins - therapeutic use</subject><subject>Biofilms - drug effects</subject><subject>Calcium Hydroxide - therapeutic use</subject><subject>Chlorhexidine - therapeutic use</subject><subject>Dental Pulp Cavity - drug effects</subject><subject>Dental Pulp Cavity - microbiology</subject><subject>Dentin - drug effects</subject><subject>Dentin - microbiology</subject><subject>dentine block</subject><subject>Dentistry</subject><subject>E. faecalis biofilms</subject><subject>Enterococcus faecalis</subject><subject>Enterococcus faecalis - drug effects</subject><subject>Gram-Positive Bacterial Infections - drug therapy</subject><subject>human β-defensin-3</subject><subject>Humans</subject><subject>Humidity</subject><subject>Materials Testing</subject><subject>Nephelometry and Turbidimetry</subject><subject>optical density</subject><subject>Root Canal Irrigants - therapeutic use</subject><subject>Sodium Chloride</subject><subject>Spectrophotometry</subject><subject>Temperature</subject><subject>Time Factors</subject><issn>0143-2885</issn><issn>1365-2591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc9OwzAMxiMEgjE48AIoRy6F_Gna9AhoGyA0hABxjNLUgUCbjqYV7LV4EJ6JwIAzvtiWf58t-UNoj5JDGuPIwdMhZYSwNTSiPBMJEwVdRyNCU54wKcUW2g7hiRAiCKebaItxkksqshHyx753jTNdWzpdY7DWGW2WuLVY48eh0R5_vCcVWPDB-YTjBSx6VwEeYvuA43jie-ha0xozBGw1GF27gCuIez1g5y2Y3rUeN20F9Q7asLoOsPuTx-huOrk9PUsur2bnp8eXiWM8Z4lJmeDMZqSyuqAlpUZLMIZlJeTC5CwtpBAlLSsjeVZaklfa5JJDIXgmSQF8jA5Wexdd-zJA6FXjgoG61h7aISjKU5kWJCXFP1CW5ZyxgkR0_wcdygYqtehco7ul-n1nBI5WwKurYfk3p0R9-aSiT-rbJ3U-ufguoiJZKVzo4e1PobtnFa_mQt3PZyo9ubm-n87mivNPqIeUmQ</recordid><startdate>201305</startdate><enddate>201305</enddate><creator>Lee, J.-K.</creator><creator>Park, Y.-J.</creator><creator>Kum, K.-Y.</creator><creator>Han, S. H.</creator><creator>Chang, S.-W.</creator><creator>Kaufman, B.</creator><creator>Jiang, J.</creator><creator>Zhu, Q.</creator><creator>Safavi, K.</creator><creator>Spångberg, L.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>201305</creationdate><title>Antimicrobial efficacy of a human β-defensin-3 peptide using an Enterococcus faecalis dentine infection model</title><author>Lee, J.-K. ; Park, Y.-J. ; Kum, K.-Y. ; Han, S. H. ; Chang, S.-W. ; Kaufman, B. ; Jiang, J. ; Zhu, Q. ; Safavi, K. ; Spångberg, L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i2372-c42532f60dfa91b11ca8ecc26be75c7249855b1bdc836bf07dac783e9536809e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Anti-Infective Agents - therapeutic use</topic><topic>Anti-Infective Agents, Local - therapeutic use</topic><topic>Antimicrobial agents</topic><topic>antimicrobial efficacy</topic><topic>beta-Defensins - therapeutic use</topic><topic>Biofilms - drug effects</topic><topic>Calcium Hydroxide - therapeutic use</topic><topic>Chlorhexidine - therapeutic use</topic><topic>Dental Pulp Cavity - drug effects</topic><topic>Dental Pulp Cavity - microbiology</topic><topic>Dentin - drug effects</topic><topic>Dentin - microbiology</topic><topic>dentine block</topic><topic>Dentistry</topic><topic>E. faecalis biofilms</topic><topic>Enterococcus faecalis</topic><topic>Enterococcus faecalis - drug effects</topic><topic>Gram-Positive Bacterial Infections - drug therapy</topic><topic>human β-defensin-3</topic><topic>Humans</topic><topic>Humidity</topic><topic>Materials Testing</topic><topic>Nephelometry and Turbidimetry</topic><topic>optical density</topic><topic>Root Canal Irrigants - therapeutic use</topic><topic>Sodium Chloride</topic><topic>Spectrophotometry</topic><topic>Temperature</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, J.-K.</creatorcontrib><creatorcontrib>Park, Y.-J.</creatorcontrib><creatorcontrib>Kum, K.-Y.</creatorcontrib><creatorcontrib>Han, S. H.</creatorcontrib><creatorcontrib>Chang, S.-W.</creatorcontrib><creatorcontrib>Kaufman, B.</creatorcontrib><creatorcontrib>Jiang, J.</creatorcontrib><creatorcontrib>Zhu, Q.</creatorcontrib><creatorcontrib>Safavi, K.</creatorcontrib><creatorcontrib>Spångberg, L.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International endodontic journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, J.-K.</au><au>Park, Y.-J.</au><au>Kum, K.-Y.</au><au>Han, S. H.</au><au>Chang, S.-W.</au><au>Kaufman, B.</au><au>Jiang, J.</au><au>Zhu, Q.</au><au>Safavi, K.</au><au>Spångberg, L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antimicrobial efficacy of a human β-defensin-3 peptide using an Enterococcus faecalis dentine infection model</atitle><jtitle>International endodontic journal</jtitle><addtitle>Int Endod J</addtitle><date>2013-05</date><risdate>2013</risdate><volume>46</volume><issue>5</issue><spage>406</spage><epage>412</epage><pages>406-412</pages><issn>0143-2885</issn><eissn>1365-2591</eissn><abstract>Aim
To assess the antibacterial efficacy of a human β‐defensin‐3 (HBD3) peptide against Enterococcus faecalis biofilms.
Methodology
Standardized human dentine blocks were infected with E. faecalis ATCC 29212 for 3 weeks. Aqueous calcium hydroxide paste (n = 12, CH), a 2% chlorhexidine gel (n = 12, CHX), an HBD3 peptide gel (n = 12) and saline (n = 12) were tested as experimental groups. A mismatched peptide gel group (n = 12, MP) and sterilized but noninoculated block group (n = 12) were included as controls. After 1 week of medication, the dentinal samples at the depth of 200 and 400 μm were collected from medicated canal lumens. Bacterial growth was assessed by spectrophotometric analysis of optical density (OD) after 72 h of incubation. Statistical analysis was performed with repeated‐measures anova and Tukey's post hoc test.
Results
The HBD3 group was associated with significantly lower OD values (P < 0.05) than the CH or CHX groups at both depths. The CH group did not differ significantly from MP or Saline group at either depth (P > 0.05). There was no significant difference (P > 0.05) in the OD values of the inner (200 μm) and outer (400 μm) dentinal samples for any group.
Conclusions
The HBD3 peptide inhibited the growth of E. faecalis biofilms in infected dentine blocks.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>23078156</pmid><doi>10.1111/iej.12002</doi><tpages>7</tpages></addata></record> |
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| subjects | Anti-Infective Agents - therapeutic use Anti-Infective Agents, Local - therapeutic use Antimicrobial agents antimicrobial efficacy beta-Defensins - therapeutic use Biofilms - drug effects Calcium Hydroxide - therapeutic use Chlorhexidine - therapeutic use Dental Pulp Cavity - drug effects Dental Pulp Cavity - microbiology Dentin - drug effects Dentin - microbiology dentine block Dentistry E. faecalis biofilms Enterococcus faecalis Enterococcus faecalis - drug effects Gram-Positive Bacterial Infections - drug therapy human β-defensin-3 Humans Humidity Materials Testing Nephelometry and Turbidimetry optical density Root Canal Irrigants - therapeutic use Sodium Chloride Spectrophotometry Temperature Time Factors |
| title | Antimicrobial efficacy of a human β-defensin-3 peptide using an Enterococcus faecalis dentine infection model |
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