Fractionation, characterization and antioxidant activity of exopolysaccharides from fermentation broth of a Cordyceps sinensis fungus
► Exopolysaccharides (EPS) were fractionated by gradient ethanol precipitation. ► Large PS with negligible protein content was precipitated with small ethanol volume. ► Smaller PS–protein complex fractions were attained at larger ethanol volume. ► Protein constituents of EPS were significant for ant...
Gespeichert in:
| Veröffentlicht in: | Process biochemistry (1991) 2013-02, Vol.48 (2), p.380-386 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 386 |
|---|---|
| container_issue | 2 |
| container_start_page | 380 |
| container_title | Process biochemistry (1991) |
| container_volume | 48 |
| creator | Huang, Qi-Lin Siu, Ka-Chai Wang, Wen-Qiang Cheung, Yi-Ching Wu, Jian-Yong |
| description | ► Exopolysaccharides (EPS) were fractionated by gradient ethanol precipitation. ► Large PS with negligible protein content was precipitated with small ethanol volume. ► Smaller PS–protein complex fractions were attained at larger ethanol volume. ► Protein constituents of EPS were significant for antioxidant activities.
Exopolysaccharide (EPS) fractions P1/5, P2/5, P1, P2 and P5 were isolated from the fermentation medium of a medicinal fungus Cordyceps sinensis by gradient precipitation with ethanol at 1/5, 2/5, 1, 2, and 5 volume ratios to the liquid medium. P1/5 and P2/5 were mainly composed of polysaccharides with negligible protein content and a large molecular or particle size (intrinsic viscosity [η] 2025mL/g; hydrodynamic radius Rh 905nm). The fraction attained at a higher ethanol volume ratio had a much higher protein and lower carbohydrate content, and a smaller molecular size. In particular, P5 was composed of mainly protein with an average molecular weight 16kDa, [η] 4.3mL/g and Rh 23.5nm. The antioxidant activities of EPS fractions showed a significant dependence on the protein content, being negligible of P1/5 and P2/5, low to moderate of P1 and P2, and very strong of P5. Gradient ethanol precipitation was proven a simple and workable method for initial fractionation of polysaccharides, proteins and their complexes with different molecular sizes and for further identification of the bioactive components. |
| doi_str_mv | 10.1016/j.procbio.2013.01.001 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1348489349</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1359511313000032</els_id><sourcerecordid>1348484370</sourcerecordid><originalsourceid>FETCH-LOGICAL-c502t-aa8f7585a3962d986592e4a5af37af0f82965d558e674d8df65bce9ec31c11fa3</originalsourceid><addsrcrecordid>eNqNkU1v1DAQhiMEEqXwExA-9tAETxwn9qmqVi0gVeIAPVuz9rj1ajfe2tmq2zv_G4f0Xg7zodHzzkjzVtVn4A1w6L9umn2Kdh1i03IQDYeGc3hTnYAaRC1ard6WXkhdSwDxvvqQ84ZzAQD8pPpzndBOIY44p3Nm73EeUArP_yYMR1eitE_Blcpm-jFMRxY9o6e4j9tjRjvLgqPMfIo75intaJyWBesUp_uZRraKyR0t7TPLYaQxh8IfxrtD_li987jN9Omlnla311e_V9_rm5_ffqwub2oreTvViMoPUkkUum-dVr3ULXUo0YsBPfeq1b10Uirqh84p53u5tqTJCrAAHsVpdbbsLQ97OFCezC5kS9stjhQP2YDoVKe06PR_op0YeEHlgtoUc07kzT6FHaajAW5mh8zGvDhkZocMB1McKrovi85jNHiXQja3vwrQc86HrgNRiIuFoPKUx0DJZBtotORCIjsZF8MrN_4CLS2peQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1348484370</pqid></control><display><type>article</type><title>Fractionation, characterization and antioxidant activity of exopolysaccharides from fermentation broth of a Cordyceps sinensis fungus</title><source>ScienceDirect Journals (5 years ago - present)</source><creator>Huang, Qi-Lin ; Siu, Ka-Chai ; Wang, Wen-Qiang ; Cheung, Yi-Ching ; Wu, Jian-Yong</creator><creatorcontrib>Huang, Qi-Lin ; Siu, Ka-Chai ; Wang, Wen-Qiang ; Cheung, Yi-Ching ; Wu, Jian-Yong</creatorcontrib><description>► Exopolysaccharides (EPS) were fractionated by gradient ethanol precipitation. ► Large PS with negligible protein content was precipitated with small ethanol volume. ► Smaller PS–protein complex fractions were attained at larger ethanol volume. ► Protein constituents of EPS were significant for antioxidant activities.
Exopolysaccharide (EPS) fractions P1/5, P2/5, P1, P2 and P5 were isolated from the fermentation medium of a medicinal fungus Cordyceps sinensis by gradient precipitation with ethanol at 1/5, 2/5, 1, 2, and 5 volume ratios to the liquid medium. P1/5 and P2/5 were mainly composed of polysaccharides with negligible protein content and a large molecular or particle size (intrinsic viscosity [η] 2025mL/g; hydrodynamic radius Rh 905nm). The fraction attained at a higher ethanol volume ratio had a much higher protein and lower carbohydrate content, and a smaller molecular size. In particular, P5 was composed of mainly protein with an average molecular weight 16kDa, [η] 4.3mL/g and Rh 23.5nm. The antioxidant activities of EPS fractions showed a significant dependence on the protein content, being negligible of P1/5 and P2/5, low to moderate of P1 and P2, and very strong of P5. Gradient ethanol precipitation was proven a simple and workable method for initial fractionation of polysaccharides, proteins and their complexes with different molecular sizes and for further identification of the bioactive components.</description><identifier>ISSN: 1359-5113</identifier><identifier>EISSN: 1873-3298</identifier><identifier>DOI: 10.1016/j.procbio.2013.01.001</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>Antioxidant activity ; carbohydrate content ; Cordyceps sinensis ; culture media ; ethanol ; Ethanol precipitation ; exopolysaccharides ; fermentation ; fractionation ; hydrodynamics ; Liquid fermentation ; medicinal fungi ; molecular weight ; Ophiocordyceps sinensis ; particle size ; Polysaccharide–protein complex ; protein content ; proteins ; viscosity</subject><ispartof>Process biochemistry (1991), 2013-02, Vol.48 (2), p.380-386</ispartof><rights>2013 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c502t-aa8f7585a3962d986592e4a5af37af0f82965d558e674d8df65bce9ec31c11fa3</citedby><cites>FETCH-LOGICAL-c502t-aa8f7585a3962d986592e4a5af37af0f82965d558e674d8df65bce9ec31c11fa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.procbio.2013.01.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids></links><search><creatorcontrib>Huang, Qi-Lin</creatorcontrib><creatorcontrib>Siu, Ka-Chai</creatorcontrib><creatorcontrib>Wang, Wen-Qiang</creatorcontrib><creatorcontrib>Cheung, Yi-Ching</creatorcontrib><creatorcontrib>Wu, Jian-Yong</creatorcontrib><title>Fractionation, characterization and antioxidant activity of exopolysaccharides from fermentation broth of a Cordyceps sinensis fungus</title><title>Process biochemistry (1991)</title><description>► Exopolysaccharides (EPS) were fractionated by gradient ethanol precipitation. ► Large PS with negligible protein content was precipitated with small ethanol volume. ► Smaller PS–protein complex fractions were attained at larger ethanol volume. ► Protein constituents of EPS were significant for antioxidant activities.
Exopolysaccharide (EPS) fractions P1/5, P2/5, P1, P2 and P5 were isolated from the fermentation medium of a medicinal fungus Cordyceps sinensis by gradient precipitation with ethanol at 1/5, 2/5, 1, 2, and 5 volume ratios to the liquid medium. P1/5 and P2/5 were mainly composed of polysaccharides with negligible protein content and a large molecular or particle size (intrinsic viscosity [η] 2025mL/g; hydrodynamic radius Rh 905nm). The fraction attained at a higher ethanol volume ratio had a much higher protein and lower carbohydrate content, and a smaller molecular size. In particular, P5 was composed of mainly protein with an average molecular weight 16kDa, [η] 4.3mL/g and Rh 23.5nm. The antioxidant activities of EPS fractions showed a significant dependence on the protein content, being negligible of P1/5 and P2/5, low to moderate of P1 and P2, and very strong of P5. Gradient ethanol precipitation was proven a simple and workable method for initial fractionation of polysaccharides, proteins and their complexes with different molecular sizes and for further identification of the bioactive components.</description><subject>Antioxidant activity</subject><subject>carbohydrate content</subject><subject>Cordyceps sinensis</subject><subject>culture media</subject><subject>ethanol</subject><subject>Ethanol precipitation</subject><subject>exopolysaccharides</subject><subject>fermentation</subject><subject>fractionation</subject><subject>hydrodynamics</subject><subject>Liquid fermentation</subject><subject>medicinal fungi</subject><subject>molecular weight</subject><subject>Ophiocordyceps sinensis</subject><subject>particle size</subject><subject>Polysaccharide–protein complex</subject><subject>protein content</subject><subject>proteins</subject><subject>viscosity</subject><issn>1359-5113</issn><issn>1873-3298</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqNkU1v1DAQhiMEEqXwExA-9tAETxwn9qmqVi0gVeIAPVuz9rj1ajfe2tmq2zv_G4f0Xg7zodHzzkjzVtVn4A1w6L9umn2Kdh1i03IQDYeGc3hTnYAaRC1ard6WXkhdSwDxvvqQ84ZzAQD8pPpzndBOIY44p3Nm73EeUArP_yYMR1eitE_Blcpm-jFMRxY9o6e4j9tjRjvLgqPMfIo75intaJyWBesUp_uZRraKyR0t7TPLYaQxh8IfxrtD_li987jN9Omlnla311e_V9_rm5_ffqwub2oreTvViMoPUkkUum-dVr3ULXUo0YsBPfeq1b10Uirqh84p53u5tqTJCrAAHsVpdbbsLQ97OFCezC5kS9stjhQP2YDoVKe06PR_op0YeEHlgtoUc07kzT6FHaajAW5mh8zGvDhkZocMB1McKrovi85jNHiXQja3vwrQc86HrgNRiIuFoPKUx0DJZBtotORCIjsZF8MrN_4CLS2peQ</recordid><startdate>20130201</startdate><enddate>20130201</enddate><creator>Huang, Qi-Lin</creator><creator>Siu, Ka-Chai</creator><creator>Wang, Wen-Qiang</creator><creator>Cheung, Yi-Ching</creator><creator>Wu, Jian-Yong</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20130201</creationdate><title>Fractionation, characterization and antioxidant activity of exopolysaccharides from fermentation broth of a Cordyceps sinensis fungus</title><author>Huang, Qi-Lin ; Siu, Ka-Chai ; Wang, Wen-Qiang ; Cheung, Yi-Ching ; Wu, Jian-Yong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c502t-aa8f7585a3962d986592e4a5af37af0f82965d558e674d8df65bce9ec31c11fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Antioxidant activity</topic><topic>carbohydrate content</topic><topic>Cordyceps sinensis</topic><topic>culture media</topic><topic>ethanol</topic><topic>Ethanol precipitation</topic><topic>exopolysaccharides</topic><topic>fermentation</topic><topic>fractionation</topic><topic>hydrodynamics</topic><topic>Liquid fermentation</topic><topic>medicinal fungi</topic><topic>molecular weight</topic><topic>Ophiocordyceps sinensis</topic><topic>particle size</topic><topic>Polysaccharide–protein complex</topic><topic>protein content</topic><topic>proteins</topic><topic>viscosity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Qi-Lin</creatorcontrib><creatorcontrib>Siu, Ka-Chai</creatorcontrib><creatorcontrib>Wang, Wen-Qiang</creatorcontrib><creatorcontrib>Cheung, Yi-Ching</creatorcontrib><creatorcontrib>Wu, Jian-Yong</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Process biochemistry (1991)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Qi-Lin</au><au>Siu, Ka-Chai</au><au>Wang, Wen-Qiang</au><au>Cheung, Yi-Ching</au><au>Wu, Jian-Yong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fractionation, characterization and antioxidant activity of exopolysaccharides from fermentation broth of a Cordyceps sinensis fungus</atitle><jtitle>Process biochemistry (1991)</jtitle><date>2013-02-01</date><risdate>2013</risdate><volume>48</volume><issue>2</issue><spage>380</spage><epage>386</epage><pages>380-386</pages><issn>1359-5113</issn><eissn>1873-3298</eissn><abstract>► Exopolysaccharides (EPS) were fractionated by gradient ethanol precipitation. ► Large PS with negligible protein content was precipitated with small ethanol volume. ► Smaller PS–protein complex fractions were attained at larger ethanol volume. ► Protein constituents of EPS were significant for antioxidant activities.
Exopolysaccharide (EPS) fractions P1/5, P2/5, P1, P2 and P5 were isolated from the fermentation medium of a medicinal fungus Cordyceps sinensis by gradient precipitation with ethanol at 1/5, 2/5, 1, 2, and 5 volume ratios to the liquid medium. P1/5 and P2/5 were mainly composed of polysaccharides with negligible protein content and a large molecular or particle size (intrinsic viscosity [η] 2025mL/g; hydrodynamic radius Rh 905nm). The fraction attained at a higher ethanol volume ratio had a much higher protein and lower carbohydrate content, and a smaller molecular size. In particular, P5 was composed of mainly protein with an average molecular weight 16kDa, [η] 4.3mL/g and Rh 23.5nm. The antioxidant activities of EPS fractions showed a significant dependence on the protein content, being negligible of P1/5 and P2/5, low to moderate of P1 and P2, and very strong of P5. Gradient ethanol precipitation was proven a simple and workable method for initial fractionation of polysaccharides, proteins and their complexes with different molecular sizes and for further identification of the bioactive components.</abstract><pub>Elsevier Ltd</pub><doi>10.1016/j.procbio.2013.01.001</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1359-5113 |
| ispartof | Process biochemistry (1991), 2013-02, Vol.48 (2), p.380-386 |
| issn | 1359-5113 1873-3298 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1348489349 |
| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Antioxidant activity carbohydrate content Cordyceps sinensis culture media ethanol Ethanol precipitation exopolysaccharides fermentation fractionation hydrodynamics Liquid fermentation medicinal fungi molecular weight Ophiocordyceps sinensis particle size Polysaccharide–protein complex protein content proteins viscosity |
| title | Fractionation, characterization and antioxidant activity of exopolysaccharides from fermentation broth of a Cordyceps sinensis fungus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T17%3A37%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Fractionation,%20characterization%20and%20antioxidant%20activity%20of%20exopolysaccharides%20from%20fermentation%20broth%20of%20a%20Cordyceps%20sinensis%20fungus&rft.jtitle=Process%20biochemistry%20(1991)&rft.au=Huang,%20Qi-Lin&rft.date=2013-02-01&rft.volume=48&rft.issue=2&rft.spage=380&rft.epage=386&rft.pages=380-386&rft.issn=1359-5113&rft.eissn=1873-3298&rft_id=info:doi/10.1016/j.procbio.2013.01.001&rft_dat=%3Cproquest_cross%3E1348484370%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1348484370&rft_id=info:pmid/&rft_els_id=S1359511313000032&rfr_iscdi=true |