Enzyme-mediated nutrient release: glucose-precursor activation by β-galactosidase to induce bacterial growth
Bacteria will gain an advantage if they are able to metabolize nutrients that are inaccessible for other bacteria. To demonstrate this principle, we developed a simple model system, which mimics how bacteria exploit natural carbon sources. A masked glucose precursor that is activated by β-galactosid...
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| Veröffentlicht in: | Organic & biomolecular chemistry 2013-05, Vol.11 (17), p.293-291 |
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| container_title | Organic & biomolecular chemistry |
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| creator | Karton-Lifshin, Naama Vogel, Uwe Sella, Eran Seeberger, Peter H Shabat, Doron Lepenies, Bernd |
| description | Bacteria will gain an advantage if they are able to metabolize nutrients that are inaccessible for other bacteria. To demonstrate this principle, we developed a simple model system, which mimics how bacteria exploit natural carbon sources. A masked glucose precursor that is activated by β-galactosidase was used as a carbon source for bacterial growth in a glucose-deficient medium. No bacterial growth was observed in the presence of control substances in which β-galactosidase mediated cleavage did not lead to glucose release. This study represents a proof-of-principle example in which a bacterium can grow in a nutrient-free medium by inducible, enzyme-mediated nutrient release from a precursor.
A masked glucose precursor that is activated by β-galactosidase was used as a carbon source for bacterial growth in a glucose-deficient medium. |
| doi_str_mv | 10.1039/c3ob27385g |
| format | Article |
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A masked glucose precursor that is activated by β-galactosidase was used as a carbon source for bacterial growth in a glucose-deficient medium.</description><subject>Bacteria</subject><subject>beta-Galactosidase - chemistry</subject><subject>beta-Galactosidase - metabolism</subject><subject>Escherichia coli - chemistry</subject><subject>Escherichia coli - enzymology</subject><subject>Escherichia coli - growth & development</subject><subject>Glucose - chemistry</subject><subject>Glucose - metabolism</subject><issn>1477-0520</issn><issn>1477-0539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0UtLHTEUB_BQKvVW3XTfEndSGM1rHnEn4gsEN7oekszJNGVmcptkLLcfyw_iZzJy7b07kSwSTn75E85B6Bslx5RweWK416zmTdl_Qgsq6rogJZefN2dGdtHXGH8TQmVdiS9ol_GSSir4Ao0X07_VCMUInVMJOjzNKTiYEg4wgIpwivthNj5CsQxg5hB9wMok96iS8xPWK_z8VPRqyDUfXZdf4OSxm7rZANa5CsGpAffB_02_9tGOVUOEg7d9Dz1cXtyfXxe3d1c352e3heFVkwoqTAfSlhVtTP49JxJECdaYWpbEWs0k8IqJkllVW1l2lRK11rVWvDHaGM730NE6dxn8nxliakcXDQyDmsDPsaVcNHlJxj5AmeBNIwTJ9OeamuBjDGDbZXCjCquWkvZ1Eu12Ehn_eMuddW7uhv5vfQaHaxCi2dxuA9plZ7P5_p7hL8sqnDs</recordid><startdate>20130507</startdate><enddate>20130507</enddate><creator>Karton-Lifshin, Naama</creator><creator>Vogel, Uwe</creator><creator>Sella, Eran</creator><creator>Seeberger, Peter H</creator><creator>Shabat, Doron</creator><creator>Lepenies, Bernd</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20130507</creationdate><title>Enzyme-mediated nutrient release: glucose-precursor activation by β-galactosidase to induce bacterial growth</title><author>Karton-Lifshin, Naama ; Vogel, Uwe ; Sella, Eran ; Seeberger, Peter H ; Shabat, Doron ; Lepenies, Bernd</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-14cde9f5618c520309e45efcc7950ffb29e362452fa7f95d6a47bb7ba38cbcc33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Bacteria</topic><topic>beta-Galactosidase - chemistry</topic><topic>beta-Galactosidase - metabolism</topic><topic>Escherichia coli - chemistry</topic><topic>Escherichia coli - enzymology</topic><topic>Escherichia coli - growth & development</topic><topic>Glucose - chemistry</topic><topic>Glucose - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karton-Lifshin, Naama</creatorcontrib><creatorcontrib>Vogel, Uwe</creatorcontrib><creatorcontrib>Sella, Eran</creatorcontrib><creatorcontrib>Seeberger, Peter H</creatorcontrib><creatorcontrib>Shabat, Doron</creatorcontrib><creatorcontrib>Lepenies, Bernd</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Organic & biomolecular chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Karton-Lifshin, Naama</au><au>Vogel, Uwe</au><au>Sella, Eran</au><au>Seeberger, Peter H</au><au>Shabat, Doron</au><au>Lepenies, Bernd</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzyme-mediated nutrient release: glucose-precursor activation by β-galactosidase to induce bacterial growth</atitle><jtitle>Organic & biomolecular chemistry</jtitle><addtitle>Org Biomol Chem</addtitle><date>2013-05-07</date><risdate>2013</risdate><volume>11</volume><issue>17</issue><spage>293</spage><epage>291</epage><pages>293-291</pages><issn>1477-0520</issn><eissn>1477-0539</eissn><abstract>Bacteria will gain an advantage if they are able to metabolize nutrients that are inaccessible for other bacteria. 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| ispartof | Organic & biomolecular chemistry, 2013-05, Vol.11 (17), p.293-291 |
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| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals; Alma/SFX Local Collection |
| subjects | Bacteria beta-Galactosidase - chemistry beta-Galactosidase - metabolism Escherichia coli - chemistry Escherichia coli - enzymology Escherichia coli - growth & development Glucose - chemistry Glucose - metabolism |
| title | Enzyme-mediated nutrient release: glucose-precursor activation by β-galactosidase to induce bacterial growth |
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