Microarray analysis of the inflammatory and immune responses in head kidney turbot leucocytes treated with resveratrol
A DNA oligo-microarray enriched in genes and involved in inflammatory and immune responses was used to evaluate the effects of resveratrol on gene expression in turbot head kidney leucocytes. Leucocytes were cultured for 3, 6 and 24h, in the presence or absence of resveratrol, or were stimulated wit...
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| Veröffentlicht in: | International immunopharmacology 2013-03, Vol.15 (3), p.588-596 |
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| creator | Domínguez, Berta Pardo, Belén G. Noia, Manuel Millán, Adrián Gómez-Tato, Antonio Martínez, Paulino Leiro, José Lamas, Jesús |
| description | A DNA oligo-microarray enriched in genes and involved in inflammatory and immune responses was used to evaluate the effects of resveratrol on gene expression in turbot head kidney leucocytes. Leucocytes were cultured for 3, 6 and 24h, in the presence or absence of resveratrol, or were stimulated with the membrane fraction of the parasite Philasterides dicentrarchi or with the membrane plus resveratrol. Gene expression changed considerably in control cells, and several of the regulated genes were related to inflammatory and immune responses and to the cytoskeleton. Similar changes in gene expression occurred in control cells and in cells stimulated with P. dicentrarchi membrane fraction. Treatment with resveratrol induced changes in the expression (mostly down-regulation) of several genes involved in immune responses and inflammation. Thus, the down-regulation of the transcription factor PU.1, pentraxin-multidomain protein, heme oxygenase 1, S100 calcium-binding protein A-16 (S100A16) and the signal transducer and activator of transcription 4 was observed after all three incubation times. The down-regulation of the suppressor of cytokine signalling 3a, LPS-induced tumour necrosis alpha, hepcidin, metallothionein, TLR8 and the calcium dependent lectin A was observed after 3 and 6h. Resveratrol also decreased the expression of CCL20, IL-8, apolipoprotein E and glutathione S-transferase after incubation for 6 and 24h, and of TNF-α after incubation for 3 and 24h. Resveratrol also induced strong regulation of several cytoskeleton-related genes. The use of the turbot oligo-microarray enabled us to discover genes whose expression was not previously suspected of being modulated by this polyphenol.
► We determine the effects of resveratrol on gene expression in turbot leucocytes. ► Cells were stimulated with P. dicentrarchi membrane or with membrane and resveratrol. ► Gene expression changed considerably with time in cultured control cells. ► Resveratrol induced regulation of genes involved in inflammation and immunity. ► Several regulated genes were not suspected of being modulated by resveratrol. |
| doi_str_mv | 10.1016/j.intimp.2013.01.024 |
| format | Article |
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► We determine the effects of resveratrol on gene expression in turbot leucocytes. ► Cells were stimulated with P. dicentrarchi membrane or with membrane and resveratrol. ► Gene expression changed considerably with time in cultured control cells. ► Resveratrol induced regulation of genes involved in inflammation and immunity. ► Several regulated genes were not suspected of being modulated by resveratrol.</description><identifier>ISSN: 1567-5769</identifier><identifier>EISSN: 1878-1705</identifier><identifier>DOI: 10.1016/j.intimp.2013.01.024</identifier><identifier>PMID: 23419489</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antigens, Protozoan - immunology ; Cells, Cultured ; Ciliophora - immunology ; Flatfishes - immunology ; Gene Expression Regulation - drug effects ; Head Kidney - drug effects ; Head Kidney - immunology ; Heme Oxygenase-1 - metabolism ; Immunity ; Immunity - drug effects ; Inflammation ; Inflammation - genetics ; Inflammation Mediators - metabolism ; Leukocytes - drug effects ; Leukocytes - immunology ; Microarray ; Microarray Analysis ; Philasterides dicentrarchi ; Proto-Oncogene Proteins - metabolism ; Resveratrol ; S100 Proteins - metabolism ; Scophthalmus maximus ; STAT4 Transcription Factor - metabolism ; Stilbenes - pharmacology ; Trans-Activators - metabolism ; Turbot</subject><ispartof>International immunopharmacology, 2013-03, Vol.15 (3), p.588-596</ispartof><rights>2013 Elsevier B.V.</rights><rights>Copyright © 2013 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c395t-583a3ec177b86c2d7961f454e5f61c98aff1ad0f9cb4fcc4da2524d8ba7e46583</citedby><cites>FETCH-LOGICAL-c395t-583a3ec177b86c2d7961f454e5f61c98aff1ad0f9cb4fcc4da2524d8ba7e46583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.intimp.2013.01.024$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23419489$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Domínguez, Berta</creatorcontrib><creatorcontrib>Pardo, Belén G.</creatorcontrib><creatorcontrib>Noia, Manuel</creatorcontrib><creatorcontrib>Millán, Adrián</creatorcontrib><creatorcontrib>Gómez-Tato, Antonio</creatorcontrib><creatorcontrib>Martínez, Paulino</creatorcontrib><creatorcontrib>Leiro, José</creatorcontrib><creatorcontrib>Lamas, Jesús</creatorcontrib><title>Microarray analysis of the inflammatory and immune responses in head kidney turbot leucocytes treated with resveratrol</title><title>International immunopharmacology</title><addtitle>Int Immunopharmacol</addtitle><description>A DNA oligo-microarray enriched in genes and involved in inflammatory and immune responses was used to evaluate the effects of resveratrol on gene expression in turbot head kidney leucocytes. Leucocytes were cultured for 3, 6 and 24h, in the presence or absence of resveratrol, or were stimulated with the membrane fraction of the parasite Philasterides dicentrarchi or with the membrane plus resveratrol. Gene expression changed considerably in control cells, and several of the regulated genes were related to inflammatory and immune responses and to the cytoskeleton. Similar changes in gene expression occurred in control cells and in cells stimulated with P. dicentrarchi membrane fraction. Treatment with resveratrol induced changes in the expression (mostly down-regulation) of several genes involved in immune responses and inflammation. Thus, the down-regulation of the transcription factor PU.1, pentraxin-multidomain protein, heme oxygenase 1, S100 calcium-binding protein A-16 (S100A16) and the signal transducer and activator of transcription 4 was observed after all three incubation times. The down-regulation of the suppressor of cytokine signalling 3a, LPS-induced tumour necrosis alpha, hepcidin, metallothionein, TLR8 and the calcium dependent lectin A was observed after 3 and 6h. Resveratrol also decreased the expression of CCL20, IL-8, apolipoprotein E and glutathione S-transferase after incubation for 6 and 24h, and of TNF-α after incubation for 3 and 24h. Resveratrol also induced strong regulation of several cytoskeleton-related genes. The use of the turbot oligo-microarray enabled us to discover genes whose expression was not previously suspected of being modulated by this polyphenol.
► We determine the effects of resveratrol on gene expression in turbot leucocytes. ► Cells were stimulated with P. dicentrarchi membrane or with membrane and resveratrol. ► Gene expression changed considerably with time in cultured control cells. ► Resveratrol induced regulation of genes involved in inflammation and immunity. ► Several regulated genes were not suspected of being modulated by resveratrol.</description><subject>Animals</subject><subject>Antigens, Protozoan - immunology</subject><subject>Cells, Cultured</subject><subject>Ciliophora - immunology</subject><subject>Flatfishes - immunology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Head Kidney - drug effects</subject><subject>Head Kidney - immunology</subject><subject>Heme Oxygenase-1 - metabolism</subject><subject>Immunity</subject><subject>Immunity - drug effects</subject><subject>Inflammation</subject><subject>Inflammation - genetics</subject><subject>Inflammation Mediators - metabolism</subject><subject>Leukocytes - drug effects</subject><subject>Leukocytes - immunology</subject><subject>Microarray</subject><subject>Microarray Analysis</subject><subject>Philasterides dicentrarchi</subject><subject>Proto-Oncogene Proteins - metabolism</subject><subject>Resveratrol</subject><subject>S100 Proteins - metabolism</subject><subject>Scophthalmus maximus</subject><subject>STAT4 Transcription Factor - metabolism</subject><subject>Stilbenes - pharmacology</subject><subject>Trans-Activators - metabolism</subject><subject>Turbot</subject><issn>1567-5769</issn><issn>1878-1705</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v3CAQhlHVqPn8B1XFsRe7YIMxl0hV1HxIqXJpzwjDoGVrmy3gjfzvy2qTHKuIA2h43hlpHoQ-U1JTQrtv29rP2U-7uiG0rQmtScM-oDPai76igvCP5c07UXHRyVN0ntKWkFJn9BM6bVpGJevlGdr_9CYGHaNesZ71uCafcHA4bwD72Y16mnQO8fBpsZ-mZQYcIe3CnCAVAm9AW_zH2xlWnJc4hIxHWEwway5AjqAzWPzs8-aQ20PUOYbxEp04PSa4erkv0O_bH79u7qvHp7uHm--PlWklzxXvW92CoUIMfWcaK2RHHeMMuOuokb12jmpLnDQDc8YwqxveMNsPWgDrSvoCfT323cXwd4GU1eSTgXHUM4QlKdqyvhxGxDvQhgrJSSMLyo5oWV1KEZzaRT_puCpK1EGO2qqjHHWQowhVRU6JfXmZsAwT2LfQq40CXB8BKCvZe4gqGQ-zAesjmKxs8P-f8A8DyaVO</recordid><startdate>201303</startdate><enddate>201303</enddate><creator>Domínguez, Berta</creator><creator>Pardo, Belén G.</creator><creator>Noia, Manuel</creator><creator>Millán, Adrián</creator><creator>Gómez-Tato, Antonio</creator><creator>Martínez, Paulino</creator><creator>Leiro, José</creator><creator>Lamas, Jesús</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>201303</creationdate><title>Microarray analysis of the inflammatory and immune responses in head kidney turbot leucocytes treated with resveratrol</title><author>Domínguez, Berta ; Pardo, Belén G. ; Noia, Manuel ; Millán, Adrián ; Gómez-Tato, Antonio ; Martínez, Paulino ; Leiro, José ; Lamas, Jesús</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c395t-583a3ec177b86c2d7961f454e5f61c98aff1ad0f9cb4fcc4da2524d8ba7e46583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Antigens, Protozoan - immunology</topic><topic>Cells, Cultured</topic><topic>Ciliophora - immunology</topic><topic>Flatfishes - immunology</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Head Kidney - drug effects</topic><topic>Head Kidney - immunology</topic><topic>Heme Oxygenase-1 - metabolism</topic><topic>Immunity</topic><topic>Immunity - drug effects</topic><topic>Inflammation</topic><topic>Inflammation - genetics</topic><topic>Inflammation Mediators - metabolism</topic><topic>Leukocytes - drug effects</topic><topic>Leukocytes - immunology</topic><topic>Microarray</topic><topic>Microarray Analysis</topic><topic>Philasterides dicentrarchi</topic><topic>Proto-Oncogene Proteins - metabolism</topic><topic>Resveratrol</topic><topic>S100 Proteins - metabolism</topic><topic>Scophthalmus maximus</topic><topic>STAT4 Transcription Factor - metabolism</topic><topic>Stilbenes - pharmacology</topic><topic>Trans-Activators - metabolism</topic><topic>Turbot</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Domínguez, Berta</creatorcontrib><creatorcontrib>Pardo, Belén G.</creatorcontrib><creatorcontrib>Noia, Manuel</creatorcontrib><creatorcontrib>Millán, Adrián</creatorcontrib><creatorcontrib>Gómez-Tato, Antonio</creatorcontrib><creatorcontrib>Martínez, Paulino</creatorcontrib><creatorcontrib>Leiro, José</creatorcontrib><creatorcontrib>Lamas, Jesús</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International immunopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Domínguez, Berta</au><au>Pardo, Belén G.</au><au>Noia, Manuel</au><au>Millán, Adrián</au><au>Gómez-Tato, Antonio</au><au>Martínez, Paulino</au><au>Leiro, José</au><au>Lamas, Jesús</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microarray analysis of the inflammatory and immune responses in head kidney turbot leucocytes treated with resveratrol</atitle><jtitle>International immunopharmacology</jtitle><addtitle>Int Immunopharmacol</addtitle><date>2013-03</date><risdate>2013</risdate><volume>15</volume><issue>3</issue><spage>588</spage><epage>596</epage><pages>588-596</pages><issn>1567-5769</issn><eissn>1878-1705</eissn><abstract>A DNA oligo-microarray enriched in genes and involved in inflammatory and immune responses was used to evaluate the effects of resveratrol on gene expression in turbot head kidney leucocytes. Leucocytes were cultured for 3, 6 and 24h, in the presence or absence of resveratrol, or were stimulated with the membrane fraction of the parasite Philasterides dicentrarchi or with the membrane plus resveratrol. Gene expression changed considerably in control cells, and several of the regulated genes were related to inflammatory and immune responses and to the cytoskeleton. Similar changes in gene expression occurred in control cells and in cells stimulated with P. dicentrarchi membrane fraction. Treatment with resveratrol induced changes in the expression (mostly down-regulation) of several genes involved in immune responses and inflammation. Thus, the down-regulation of the transcription factor PU.1, pentraxin-multidomain protein, heme oxygenase 1, S100 calcium-binding protein A-16 (S100A16) and the signal transducer and activator of transcription 4 was observed after all three incubation times. The down-regulation of the suppressor of cytokine signalling 3a, LPS-induced tumour necrosis alpha, hepcidin, metallothionein, TLR8 and the calcium dependent lectin A was observed after 3 and 6h. Resveratrol also decreased the expression of CCL20, IL-8, apolipoprotein E and glutathione S-transferase after incubation for 6 and 24h, and of TNF-α after incubation for 3 and 24h. Resveratrol also induced strong regulation of several cytoskeleton-related genes. The use of the turbot oligo-microarray enabled us to discover genes whose expression was not previously suspected of being modulated by this polyphenol.
► We determine the effects of resveratrol on gene expression in turbot leucocytes. ► Cells were stimulated with P. dicentrarchi membrane or with membrane and resveratrol. ► Gene expression changed considerably with time in cultured control cells. ► Resveratrol induced regulation of genes involved in inflammation and immunity. ► Several regulated genes were not suspected of being modulated by resveratrol.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>23419489</pmid><doi>10.1016/j.intimp.2013.01.024</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Antigens, Protozoan - immunology Cells, Cultured Ciliophora - immunology Flatfishes - immunology Gene Expression Regulation - drug effects Head Kidney - drug effects Head Kidney - immunology Heme Oxygenase-1 - metabolism Immunity Immunity - drug effects Inflammation Inflammation - genetics Inflammation Mediators - metabolism Leukocytes - drug effects Leukocytes - immunology Microarray Microarray Analysis Philasterides dicentrarchi Proto-Oncogene Proteins - metabolism Resveratrol S100 Proteins - metabolism Scophthalmus maximus STAT4 Transcription Factor - metabolism Stilbenes - pharmacology Trans-Activators - metabolism Turbot |
| title | Microarray analysis of the inflammatory and immune responses in head kidney turbot leucocytes treated with resveratrol |
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