The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest
Abstract Objective The purpose of this study was to investigate the neuroprotective effects of intraperitoneal injection of hydrogen (H2 ) in rabbits with cardiac arrest (CA). Methods A rabbit model of CA was established by the delivery of alternating current between the esophagus and chest wall to...
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Veröffentlicht in: | Resuscitation 2013-05, Vol.84 (5), p.690-695 |
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description | Abstract Objective The purpose of this study was to investigate the neuroprotective effects of intraperitoneal injection of hydrogen (H2 ) in rabbits with cardiac arrest (CA). Methods A rabbit model of CA was established by the delivery of alternating current between the esophagus and chest wall to induce ventricular fibrillation. Before CA, the animals were randomly divided into four groups: a sham group (no CA), a CA group, a CA + low dose (10 ml/kg) H2 group (CA + H2 group 1), and a CA + high dose (20 ml/kg) H2 group (CA + H2 group 2). In the first experiment, animals were observed for 72 h after the restoration of spontaneous circulation (ROSC). The neurological scores were assessed at 24, 48 and 72 h after ROSC. The rabbits that survived until 72 h were sacrificed using an overdose of anesthetic, and the brain tissues were collected and Nissl-stained to observe nerve cell damage in the hippocampal CA1 area. In addition, TUNEL assay was performed to detect apoptosis. In the second experiment, animals were observed for 6 h after ROSC. Blood samples and brain hippocampal tissues were collected, and differences in oxidative stress indicators were compared among the four groups. Results Intraperitoneal injection of H2 improved the 72-h survival rate and neurological scores, reduced neuronal injury and inhibited neuronal apoptosis. Intraperitoneal injection of H2 reduced oxidative stress indicators in the plasma and hippocampal tissues and enhanced antioxidant enzyme activity. No significant difference was observed between the two CA groups treated with different doses of H2. Conclusions Intraperitoneal injection of H2 is a novel hydrogen administration method and can reduce cerebral ischemia-reperfusion injury and improve the prognosis of cardiopulmonary cerebral resuscitation in a rabbit model of CA. |
doi_str_mv | 10.1016/j.resuscitation.2012.10.018 |
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fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1347259284</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S0300957212008726</els_id><sourcerecordid>1347259284</sourcerecordid><originalsourceid>FETCH-LOGICAL-c438t-67e585a182dfd7d517d285ac86fbb8519f5b7103564c763d4366a29f3a716ba53</originalsourceid><addsrcrecordid>eNqNkU1v1DAQhi0EokvhL6BIXLhk8UccO0JCQlX5kCpxaDlbjj1mHbL2Yjut9t_X0RYkeurJHs8773ieQegdwVuCSf9h2ibISza-6OJj2FJMaM1sMZHP0IZIwVrCBX6ONphh3A5c0DP0KucJY8z4IF6iM8oIlrTDG2RudtAEWFI8pFjAFH8LDThXb7mJrvGhJH2A5EsMoOcaT6sohjW5O9oUf0Gor03S4-hrzZ0vu8boZL02jU71q-U1euH0nOHNw3mOfn65vLn41l79-Pr94vNVazomS9sL4JJrIql1VlhOhKU1NrJ34yg5GRwfBakj9J0RPbMd63tNB8e0IP2oOTtH70--dZQ_S22s9j4bmGcdIC5ZEdYJygcquyr9eJKaFHNO4NQh-b1OR0WwWimrSf1HWa2U12SlXKvfPjRaxj3Yf7V_sVbB5UkAddxbD0lVIwgGrE8Vn7LRP7HRp0c-ZvbBGz3_hiPkKS4pVKKKqEwVVtfrwtd9E4qxFLRn97DqrHI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1347259284</pqid></control><display><type>article</type><title>The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Huang, Guoqing ; Zhou, Jun ; Zhan, Wei ; Xiong, Yan ; Hu, Chunlin ; Li, Xiangmin ; Li, Xin ; Li, Yingqing ; Liao, Xiaoxing</creator><creatorcontrib>Huang, Guoqing ; Zhou, Jun ; Zhan, Wei ; Xiong, Yan ; Hu, Chunlin ; Li, Xiangmin ; Li, Xin ; Li, Yingqing ; Liao, Xiaoxing</creatorcontrib><description>Abstract Objective The purpose of this study was to investigate the neuroprotective effects of intraperitoneal injection of hydrogen (H2 ) in rabbits with cardiac arrest (CA). Methods A rabbit model of CA was established by the delivery of alternating current between the esophagus and chest wall to induce ventricular fibrillation. Before CA, the animals were randomly divided into four groups: a sham group (no CA), a CA group, a CA + low dose (10 ml/kg) H2 group (CA + H2 group 1), and a CA + high dose (20 ml/kg) H2 group (CA + H2 group 2). In the first experiment, animals were observed for 72 h after the restoration of spontaneous circulation (ROSC). The neurological scores were assessed at 24, 48 and 72 h after ROSC. The rabbits that survived until 72 h were sacrificed using an overdose of anesthetic, and the brain tissues were collected and Nissl-stained to observe nerve cell damage in the hippocampal CA1 area. In addition, TUNEL assay was performed to detect apoptosis. In the second experiment, animals were observed for 6 h after ROSC. Blood samples and brain hippocampal tissues were collected, and differences in oxidative stress indicators were compared among the four groups. Results Intraperitoneal injection of H2 improved the 72-h survival rate and neurological scores, reduced neuronal injury and inhibited neuronal apoptosis. Intraperitoneal injection of H2 reduced oxidative stress indicators in the plasma and hippocampal tissues and enhanced antioxidant enzyme activity. No significant difference was observed between the two CA groups treated with different doses of H2. Conclusions Intraperitoneal injection of H2 is a novel hydrogen administration method and can reduce cerebral ischemia-reperfusion injury and improve the prognosis of cardiopulmonary cerebral resuscitation in a rabbit model of CA.</description><identifier>ISSN: 0300-9572</identifier><identifier>EISSN: 1873-1570</identifier><identifier>DOI: 10.1016/j.resuscitation.2012.10.018</identifier><identifier>PMID: 23108240</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Animals ; Apoptosis - drug effects ; Brain - drug effects ; Brain - physiopathology ; Cardiac arrest ; Cardiopulmonary resuscitation ; Cardiopulmonary Resuscitation - methods ; Emergency ; Heart Arrest - drug therapy ; Heart Arrest - physiopathology ; Hydrogen ; Hydrogen - administration & dosage ; In Situ Nick-End Labeling ; Injections, Intraperitoneal ; Neuroprotective Agents - administration & dosage ; Oxidative stress ; Oxidative Stress - drug effects ; Rabbits</subject><ispartof>Resuscitation, 2013-05, Vol.84 (5), p.690-695</ispartof><rights>Elsevier Ireland Ltd</rights><rights>2012 Elsevier Ireland Ltd</rights><rights>Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-67e585a182dfd7d517d285ac86fbb8519f5b7103564c763d4366a29f3a716ba53</citedby><cites>FETCH-LOGICAL-c438t-67e585a182dfd7d517d285ac86fbb8519f5b7103564c763d4366a29f3a716ba53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.resuscitation.2012.10.018$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23108240$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Guoqing</creatorcontrib><creatorcontrib>Zhou, Jun</creatorcontrib><creatorcontrib>Zhan, Wei</creatorcontrib><creatorcontrib>Xiong, Yan</creatorcontrib><creatorcontrib>Hu, Chunlin</creatorcontrib><creatorcontrib>Li, Xiangmin</creatorcontrib><creatorcontrib>Li, Xin</creatorcontrib><creatorcontrib>Li, Yingqing</creatorcontrib><creatorcontrib>Liao, Xiaoxing</creatorcontrib><title>The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest</title><title>Resuscitation</title><addtitle>Resuscitation</addtitle><description>Abstract Objective The purpose of this study was to investigate the neuroprotective effects of intraperitoneal injection of hydrogen (H2 ) in rabbits with cardiac arrest (CA). Methods A rabbit model of CA was established by the delivery of alternating current between the esophagus and chest wall to induce ventricular fibrillation. Before CA, the animals were randomly divided into four groups: a sham group (no CA), a CA group, a CA + low dose (10 ml/kg) H2 group (CA + H2 group 1), and a CA + high dose (20 ml/kg) H2 group (CA + H2 group 2). In the first experiment, animals were observed for 72 h after the restoration of spontaneous circulation (ROSC). The neurological scores were assessed at 24, 48 and 72 h after ROSC. The rabbits that survived until 72 h were sacrificed using an overdose of anesthetic, and the brain tissues were collected and Nissl-stained to observe nerve cell damage in the hippocampal CA1 area. In addition, TUNEL assay was performed to detect apoptosis. In the second experiment, animals were observed for 6 h after ROSC. Blood samples and brain hippocampal tissues were collected, and differences in oxidative stress indicators were compared among the four groups. Results Intraperitoneal injection of H2 improved the 72-h survival rate and neurological scores, reduced neuronal injury and inhibited neuronal apoptosis. Intraperitoneal injection of H2 reduced oxidative stress indicators in the plasma and hippocampal tissues and enhanced antioxidant enzyme activity. No significant difference was observed between the two CA groups treated with different doses of H2. Conclusions Intraperitoneal injection of H2 is a novel hydrogen administration method and can reduce cerebral ischemia-reperfusion injury and improve the prognosis of cardiopulmonary cerebral resuscitation in a rabbit model of CA.</description><subject>Animals</subject><subject>Apoptosis - drug effects</subject><subject>Brain - drug effects</subject><subject>Brain - physiopathology</subject><subject>Cardiac arrest</subject><subject>Cardiopulmonary resuscitation</subject><subject>Cardiopulmonary Resuscitation - methods</subject><subject>Emergency</subject><subject>Heart Arrest - drug therapy</subject><subject>Heart Arrest - physiopathology</subject><subject>Hydrogen</subject><subject>Hydrogen - administration & dosage</subject><subject>In Situ Nick-End Labeling</subject><subject>Injections, Intraperitoneal</subject><subject>Neuroprotective Agents - administration & dosage</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Rabbits</subject><issn>0300-9572</issn><issn>1873-1570</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0EokvhL6BIXLhk8UccO0JCQlX5kCpxaDlbjj1mHbL2Yjut9t_X0RYkeurJHs8773ieQegdwVuCSf9h2ibISza-6OJj2FJMaM1sMZHP0IZIwVrCBX6ONphh3A5c0DP0KucJY8z4IF6iM8oIlrTDG2RudtAEWFI8pFjAFH8LDThXb7mJrvGhJH2A5EsMoOcaT6sohjW5O9oUf0Gor03S4-hrzZ0vu8boZL02jU71q-U1euH0nOHNw3mOfn65vLn41l79-Pr94vNVazomS9sL4JJrIql1VlhOhKU1NrJ34yg5GRwfBakj9J0RPbMd63tNB8e0IP2oOTtH70--dZQ_S22s9j4bmGcdIC5ZEdYJygcquyr9eJKaFHNO4NQh-b1OR0WwWimrSf1HWa2U12SlXKvfPjRaxj3Yf7V_sVbB5UkAddxbD0lVIwgGrE8Vn7LRP7HRp0c-ZvbBGz3_hiPkKS4pVKKKqEwVVtfrwtd9E4qxFLRn97DqrHI</recordid><startdate>20130501</startdate><enddate>20130501</enddate><creator>Huang, Guoqing</creator><creator>Zhou, Jun</creator><creator>Zhan, Wei</creator><creator>Xiong, Yan</creator><creator>Hu, Chunlin</creator><creator>Li, Xiangmin</creator><creator>Li, Xin</creator><creator>Li, Yingqing</creator><creator>Liao, Xiaoxing</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130501</creationdate><title>The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest</title><author>Huang, Guoqing ; Zhou, Jun ; Zhan, Wei ; Xiong, Yan ; Hu, Chunlin ; Li, Xiangmin ; Li, Xin ; Li, Yingqing ; Liao, Xiaoxing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-67e585a182dfd7d517d285ac86fbb8519f5b7103564c763d4366a29f3a716ba53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Apoptosis - drug effects</topic><topic>Brain - drug effects</topic><topic>Brain - physiopathology</topic><topic>Cardiac arrest</topic><topic>Cardiopulmonary resuscitation</topic><topic>Cardiopulmonary Resuscitation - methods</topic><topic>Emergency</topic><topic>Heart Arrest - drug therapy</topic><topic>Heart Arrest - physiopathology</topic><topic>Hydrogen</topic><topic>Hydrogen - administration & dosage</topic><topic>In Situ Nick-End Labeling</topic><topic>Injections, Intraperitoneal</topic><topic>Neuroprotective Agents - administration & dosage</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>Rabbits</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Guoqing</creatorcontrib><creatorcontrib>Zhou, Jun</creatorcontrib><creatorcontrib>Zhan, Wei</creatorcontrib><creatorcontrib>Xiong, Yan</creatorcontrib><creatorcontrib>Hu, Chunlin</creatorcontrib><creatorcontrib>Li, Xiangmin</creatorcontrib><creatorcontrib>Li, Xin</creatorcontrib><creatorcontrib>Li, Yingqing</creatorcontrib><creatorcontrib>Liao, Xiaoxing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Resuscitation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Guoqing</au><au>Zhou, Jun</au><au>Zhan, Wei</au><au>Xiong, Yan</au><au>Hu, Chunlin</au><au>Li, Xiangmin</au><au>Li, Xin</au><au>Li, Yingqing</au><au>Liao, Xiaoxing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest</atitle><jtitle>Resuscitation</jtitle><addtitle>Resuscitation</addtitle><date>2013-05-01</date><risdate>2013</risdate><volume>84</volume><issue>5</issue><spage>690</spage><epage>695</epage><pages>690-695</pages><issn>0300-9572</issn><eissn>1873-1570</eissn><abstract>Abstract Objective The purpose of this study was to investigate the neuroprotective effects of intraperitoneal injection of hydrogen (H2 ) in rabbits with cardiac arrest (CA). Methods A rabbit model of CA was established by the delivery of alternating current between the esophagus and chest wall to induce ventricular fibrillation. Before CA, the animals were randomly divided into four groups: a sham group (no CA), a CA group, a CA + low dose (10 ml/kg) H2 group (CA + H2 group 1), and a CA + high dose (20 ml/kg) H2 group (CA + H2 group 2). In the first experiment, animals were observed for 72 h after the restoration of spontaneous circulation (ROSC). The neurological scores were assessed at 24, 48 and 72 h after ROSC. The rabbits that survived until 72 h were sacrificed using an overdose of anesthetic, and the brain tissues were collected and Nissl-stained to observe nerve cell damage in the hippocampal CA1 area. In addition, TUNEL assay was performed to detect apoptosis. In the second experiment, animals were observed for 6 h after ROSC. Blood samples and brain hippocampal tissues were collected, and differences in oxidative stress indicators were compared among the four groups. Results Intraperitoneal injection of H2 improved the 72-h survival rate and neurological scores, reduced neuronal injury and inhibited neuronal apoptosis. Intraperitoneal injection of H2 reduced oxidative stress indicators in the plasma and hippocampal tissues and enhanced antioxidant enzyme activity. No significant difference was observed between the two CA groups treated with different doses of H2. Conclusions Intraperitoneal injection of H2 is a novel hydrogen administration method and can reduce cerebral ischemia-reperfusion injury and improve the prognosis of cardiopulmonary cerebral resuscitation in a rabbit model of CA.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>23108240</pmid><doi>10.1016/j.resuscitation.2012.10.018</doi><tpages>6</tpages></addata></record> |
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subjects | Animals Apoptosis - drug effects Brain - drug effects Brain - physiopathology Cardiac arrest Cardiopulmonary resuscitation Cardiopulmonary Resuscitation - methods Emergency Heart Arrest - drug therapy Heart Arrest - physiopathology Hydrogen Hydrogen - administration & dosage In Situ Nick-End Labeling Injections, Intraperitoneal Neuroprotective Agents - administration & dosage Oxidative stress Oxidative Stress - drug effects Rabbits |
title | The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest |
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