Identification of a Genetic Determinant in Clinical Enterococcus faecium Strains That Contributes to Intestinal Colonization During Antibiotic Treatment
Intestinal colonization by antibiotic-resistant Enterococcus faecium is the first step in a process that can lead to infections in hospitalized patients. By comparative genome analysis and subsequent polymerase chain reaction screening, we identified a locus that encodes a putative phosphotransferas...
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| Veröffentlicht in: | The Journal of infectious diseases 2013-06, Vol.207 (11), p.1780-1786 |
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| container_title | The Journal of infectious diseases |
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| creator | Zhang, Xinglin Top, Janetta de Been, Mark Bierschenk, Damien Rogers, Malbert Leendertse, Masja Bonten, Marc J. M. van der Poll, Tom Willems, Rob J. L. van Schaik, Willem |
| description | Intestinal colonization by antibiotic-resistant Enterococcus faecium is the first step in a process that can lead to infections in hospitalized patients. By comparative genome analysis and subsequent polymerase chain reaction screening, we identified a locus that encodes a putative phosphotransferase system (PTS). The PTS locus was widespread in isolates from hospital outbreaks of infection (84.2%) and nonoutbreak clinical infections (66.0%) but absent from human commensal isolates. Deletion of pstD, which is predicted to encode the enzyme IID subunit of this PTS, significantly impaired the ability of E. faecium to colonize the murine intestinal tract during antibiotic treatment. This is the first description of a determinant that contributes to intestinal colonization in clinical E. faecium strains. |
| doi_str_mv | 10.1093/infdis/jit076 |
| format | Article |
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M. ; van der Poll, Tom ; Willems, Rob J. L. ; van Schaik, Willem</creator><creatorcontrib>Zhang, Xinglin ; Top, Janetta ; de Been, Mark ; Bierschenk, Damien ; Rogers, Malbert ; Leendertse, Masja ; Bonten, Marc J. M. ; van der Poll, Tom ; Willems, Rob J. L. ; van Schaik, Willem</creatorcontrib><description>Intestinal colonization by antibiotic-resistant Enterococcus faecium is the first step in a process that can lead to infections in hospitalized patients. By comparative genome analysis and subsequent polymerase chain reaction screening, we identified a locus that encodes a putative phosphotransferase system (PTS). The PTS locus was widespread in isolates from hospital outbreaks of infection (84.2%) and nonoutbreak clinical infections (66.0%) but absent from human commensal isolates. Deletion of pstD, which is predicted to encode the enzyme IID subunit of this PTS, significantly impaired the ability of E. faecium to colonize the murine intestinal tract during antibiotic treatment. This is the first description of a determinant that contributes to intestinal colonization in clinical E. faecium strains.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/jit076</identifier><identifier>PMID: 23447698</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Animals ; Anti-Bacterial Agents - administration & dosage ; Antibiotics ; BACTERIA ; Bacteriology ; Biological and medical sciences ; Carrier State - microbiology ; Colon ; Commensals ; Disease Models, Animal ; Enterococcus faecium ; Enterococcus faecium - genetics ; Enterococcus faecium - isolation & purification ; Enterococcus faecium - pathogenicity ; Fundamental and applied biological sciences. 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M.</creatorcontrib><creatorcontrib>van der Poll, Tom</creatorcontrib><creatorcontrib>Willems, Rob J. L.</creatorcontrib><creatorcontrib>van Schaik, Willem</creatorcontrib><title>Identification of a Genetic Determinant in Clinical Enterococcus faecium Strains That Contributes to Intestinal Colonization During Antibiotic Treatment</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>Intestinal colonization by antibiotic-resistant Enterococcus faecium is the first step in a process that can lead to infections in hospitalized patients. By comparative genome analysis and subsequent polymerase chain reaction screening, we identified a locus that encodes a putative phosphotransferase system (PTS). The PTS locus was widespread in isolates from hospital outbreaks of infection (84.2%) and nonoutbreak clinical infections (66.0%) but absent from human commensal isolates. Deletion of pstD, which is predicted to encode the enzyme IID subunit of this PTS, significantly impaired the ability of E. faecium to colonize the murine intestinal tract during antibiotic treatment. This is the first description of a determinant that contributes to intestinal colonization in clinical E. faecium strains.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - administration & dosage</subject><subject>Antibiotics</subject><subject>BACTERIA</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Carrier State - microbiology</subject><subject>Colon</subject><subject>Commensals</subject><subject>Disease Models, Animal</subject><subject>Enterococcus faecium</subject><subject>Enterococcus faecium - genetics</subject><subject>Enterococcus faecium - isolation & purification</subject><subject>Enterococcus faecium - pathogenicity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gastrointestinal Tract - microbiology</subject><subject>Gene Deletion</subject><subject>Genetics</subject><subject>Genomes</subject><subject>Gram-Positive Bacterial Infections - microbiology</subject><subject>Humans</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbial colonization</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Multigene family</subject><subject>Phosphotransferases - genetics</subject><subject>Trucks</subject><subject>Virulence Factors - genetics</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkU9v1DAQxS0EokvhyBHkCxKXUP9bxzlW21JWqsSB5Rw5zhhmldjFdg7lk_Bx8SpLxcnS-DfvPc0j5C1nnzjr5BUGP2K-OmJhrX5GNnwr20ZrLp-TDWNCNNx03QV5lfORMaakbl-SCyGVanVnNuTPfoRQ0KOzBWOg0VNL7yBAQUdvoECaMdhQKAa6mzBUbqK3oc6ji84tmXoLDpeZfivJYsj08NMWuouhJByWApmWSPd1IZcqNNWfKQb8vbrdLAnDD3pdEwwYT5aHBLbMNdJr8sLbKcOb83tJvn--Pey-NPdf7_a76_vGSaNKI0F4PUhghrtRtEZoDQbYKGDwYKTRknUagAmrR6NMJzlwz6T1vB3U0HF5ST6uug8p_lpqyn7G7GCabIC45J5L1Yqt4XJb0WZFXYo5J_D9Q8LZpsees_5URr-W0a9lVP79WXoZZhif6H_Xr8CHM2BzvatPNri6_sS1UnWcn4zfrdwxl5j-15GKCSP_AkpsoUg</recordid><startdate>20130601</startdate><enddate>20130601</enddate><creator>Zhang, Xinglin</creator><creator>Top, Janetta</creator><creator>de Been, Mark</creator><creator>Bierschenk, Damien</creator><creator>Rogers, Malbert</creator><creator>Leendertse, Masja</creator><creator>Bonten, Marc J. M.</creator><creator>van der Poll, Tom</creator><creator>Willems, Rob J. L.</creator><creator>van Schaik, Willem</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130601</creationdate><title>Identification of a Genetic Determinant in Clinical Enterococcus faecium Strains That Contributes to Intestinal Colonization During Antibiotic Treatment</title><author>Zhang, Xinglin ; Top, Janetta ; de Been, Mark ; Bierschenk, Damien ; Rogers, Malbert ; Leendertse, Masja ; Bonten, Marc J. M. ; van der Poll, Tom ; Willems, Rob J. 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Psychology</topic><topic>Gastrointestinal Tract - microbiology</topic><topic>Gene Deletion</topic><topic>Genetics</topic><topic>Genomes</topic><topic>Gram-Positive Bacterial Infections - microbiology</topic><topic>Humans</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbial colonization</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Multigene family</topic><topic>Phosphotransferases - genetics</topic><topic>Trucks</topic><topic>Virulence Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Xinglin</creatorcontrib><creatorcontrib>Top, Janetta</creatorcontrib><creatorcontrib>de Been, Mark</creatorcontrib><creatorcontrib>Bierschenk, Damien</creatorcontrib><creatorcontrib>Rogers, Malbert</creatorcontrib><creatorcontrib>Leendertse, Masja</creatorcontrib><creatorcontrib>Bonten, Marc J. 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| ispartof | The Journal of infectious diseases, 2013-06, Vol.207 (11), p.1780-1786 |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection; JSTOR |
| subjects | Animals Anti-Bacterial Agents - administration & dosage Antibiotics BACTERIA Bacteriology Biological and medical sciences Carrier State - microbiology Colon Commensals Disease Models, Animal Enterococcus faecium Enterococcus faecium - genetics Enterococcus faecium - isolation & purification Enterococcus faecium - pathogenicity Fundamental and applied biological sciences. Psychology Gastrointestinal Tract - microbiology Gene Deletion Genetics Genomes Gram-Positive Bacterial Infections - microbiology Humans Infections Infectious diseases Male Medical sciences Mice Mice, Inbred BALB C Microbial colonization Microbiology Miscellaneous Multigene family Phosphotransferases - genetics Trucks Virulence Factors - genetics |
| title | Identification of a Genetic Determinant in Clinical Enterococcus faecium Strains That Contributes to Intestinal Colonization During Antibiotic Treatment |
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