Oligosaccharide from apple induces apoptosis and cell cycle arrest in HT29 human colon cancer cells
It is reported that apple polysaccharide can prevent colon cancer growth and impede colon cancer progression. Apple oligosaccharide was prepared by the combination of alkaline hydrolysis and enzymolysis of apple polysaccharides, and purified by anion column chromatography. The aim of this study is t...
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| Veröffentlicht in: | International journal of biological macromolecules 2013-06, Vol.57, p.245-254 |
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| container_title | International journal of biological macromolecules |
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| creator | Li, Qian Zhou, Siyuan Jing, Juan Yang, Tiehong Duan, Shuangyan Wang, Zhongfu Mei, Qibing Liu, Li |
| description | It is reported that apple polysaccharide can prevent colon cancer growth and impede colon cancer progression. Apple oligosaccharide was prepared by the combination of alkaline hydrolysis and enzymolysis of apple polysaccharides, and purified by anion column chromatography. The aim of this study is to explore the effect of apple oligosaccharide on the cellular viability of human colon carcinoma cells (HT29 cells) and its mechanism. The results showed that apple oligosaccharide decreased the cellular viability of HT29 cells in dose-dependent manner. Meanwhile it enhanced the expression of Bax; and decreased the levels of Bcl-2 and Bcl-xl. Apple oligosaccharide induced cell cycle arrest in S phase, which correlated with the decreased expression of Cdk 2 and cyclin B1. These results indicated that apple oligosaccharide attenuated HT29 cell viability by inducing cell apoptosis and cell cycle arrest. Apple oligosaccharide is a potential chemoprevention agent or anti-tumor agent and is worthy of further study. |
| doi_str_mv | 10.1016/j.ijbiomac.2013.03.034 |
| format | Article |
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Apple oligosaccharide was prepared by the combination of alkaline hydrolysis and enzymolysis of apple polysaccharides, and purified by anion column chromatography. The aim of this study is to explore the effect of apple oligosaccharide on the cellular viability of human colon carcinoma cells (HT29 cells) and its mechanism. The results showed that apple oligosaccharide decreased the cellular viability of HT29 cells in dose-dependent manner. Meanwhile it enhanced the expression of Bax; and decreased the levels of Bcl-2 and Bcl-xl. Apple oligosaccharide induced cell cycle arrest in S phase, which correlated with the decreased expression of Cdk 2 and cyclin B1. These results indicated that apple oligosaccharide attenuated HT29 cell viability by inducing cell apoptosis and cell cycle arrest. Apple oligosaccharide is a potential chemoprevention agent or anti-tumor agent and is worthy of further study.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2013.03.034</identifier><identifier>PMID: 23511050</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>alkaline hydrolysis ; antineoplastic agents ; Antineoplastic Agents - chemistry ; Antineoplastic Agents - pharmacology ; Apoptosis ; Apoptosis - drug effects ; apples ; bcl-X Protein - metabolism ; Cell cycle arrest ; cell cycle checkpoints ; Cell Cycle Checkpoints - drug effects ; Cell Line, Tumor ; Cell Survival - drug effects ; cell viability ; chemoprevention ; chromatography ; Colonic Neoplasms - drug therapy ; Colonic Neoplasms - metabolism ; Colonic Neoplasms - pathology ; colorectal neoplasms ; Cyclin B1 - metabolism ; Cyclin-Dependent Kinase 2 - metabolism ; cyclins ; dose response ; Humans ; interphase ; Malus - chemistry ; neoplasm cells ; Oligosaccharide ; oligosaccharides ; Oligosaccharides - chemistry ; Oligosaccharides - pharmacology ; polysaccharides</subject><ispartof>International journal of biological macromolecules, 2013-06, Vol.57, p.245-254</ispartof><rights>2013 Elsevier B.V.</rights><rights>Copyright © 2013 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-f7c525fdd126035d83827658bb3c1cd5624abef29825977f26a285fda94e0f463</citedby><cites>FETCH-LOGICAL-c392t-f7c525fdd126035d83827658bb3c1cd5624abef29825977f26a285fda94e0f463</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0141813013001207$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23511050$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Qian</creatorcontrib><creatorcontrib>Zhou, Siyuan</creatorcontrib><creatorcontrib>Jing, Juan</creatorcontrib><creatorcontrib>Yang, Tiehong</creatorcontrib><creatorcontrib>Duan, Shuangyan</creatorcontrib><creatorcontrib>Wang, Zhongfu</creatorcontrib><creatorcontrib>Mei, Qibing</creatorcontrib><creatorcontrib>Liu, Li</creatorcontrib><title>Oligosaccharide from apple induces apoptosis and cell cycle arrest in HT29 human colon cancer cells</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>It is reported that apple polysaccharide can prevent colon cancer growth and impede colon cancer progression. Apple oligosaccharide was prepared by the combination of alkaline hydrolysis and enzymolysis of apple polysaccharides, and purified by anion column chromatography. The aim of this study is to explore the effect of apple oligosaccharide on the cellular viability of human colon carcinoma cells (HT29 cells) and its mechanism. The results showed that apple oligosaccharide decreased the cellular viability of HT29 cells in dose-dependent manner. Meanwhile it enhanced the expression of Bax; and decreased the levels of Bcl-2 and Bcl-xl. Apple oligosaccharide induced cell cycle arrest in S phase, which correlated with the decreased expression of Cdk 2 and cyclin B1. These results indicated that apple oligosaccharide attenuated HT29 cell viability by inducing cell apoptosis and cell cycle arrest. Apple oligosaccharide is a potential chemoprevention agent or anti-tumor agent and is worthy of further study.</description><subject>alkaline hydrolysis</subject><subject>antineoplastic agents</subject><subject>Antineoplastic Agents - chemistry</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>apples</subject><subject>bcl-X Protein - metabolism</subject><subject>Cell cycle arrest</subject><subject>cell cycle checkpoints</subject><subject>Cell Cycle Checkpoints - drug effects</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival - drug effects</subject><subject>cell viability</subject><subject>chemoprevention</subject><subject>chromatography</subject><subject>Colonic Neoplasms - drug therapy</subject><subject>Colonic Neoplasms - metabolism</subject><subject>Colonic Neoplasms - pathology</subject><subject>colorectal neoplasms</subject><subject>Cyclin B1 - metabolism</subject><subject>Cyclin-Dependent Kinase 2 - metabolism</subject><subject>cyclins</subject><subject>dose response</subject><subject>Humans</subject><subject>interphase</subject><subject>Malus - chemistry</subject><subject>neoplasm cells</subject><subject>Oligosaccharide</subject><subject>oligosaccharides</subject><subject>Oligosaccharides - chemistry</subject><subject>Oligosaccharides - pharmacology</subject><subject>polysaccharides</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKxDAUhoMoOl5eQbt00zGXJm13iqgjDLhQ1yE9SWYytE1NpsK8vamjboVDbnzn5OdD6JLgOcFE3GzmbtM43ymYU0zYHE9VHKAZqco6xxizQzTDpCB5RRg-QacxbtKr4KQ6RieUcUIwxzMEL61b-agA1io4bTIbfJepYWhN5no9gonp5oetjy6dep2BadsMdpAAFYKJ28RlizdaZ-uxU30GvvVpVT2Y8A3Hc3RkVRvNxc9-ht4fH97uF_ny5en5_m6ZA6vpNrclcMqt1oQKzLiuWEVLwaumYUBAc0EL1RhL64ryuiwtFYpWiVd1YbAtBDtD1_u5Q_AfY0omOxenBKo3foySsIJzwrjgCRV7FIKPMRgrh-A6FXaSYDkJlhv5K1hOgiWeqkiNlz9_jE1n9F_br9EEXO0Bq7xUq-CifH9NE0Syz2tKp5i3e8IkF5_OBBnBmaRLu2BgK7V3_6X4Ar_jmGY</recordid><startdate>20130601</startdate><enddate>20130601</enddate><creator>Li, Qian</creator><creator>Zhou, Siyuan</creator><creator>Jing, Juan</creator><creator>Yang, Tiehong</creator><creator>Duan, Shuangyan</creator><creator>Wang, Zhongfu</creator><creator>Mei, Qibing</creator><creator>Liu, Li</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130601</creationdate><title>Oligosaccharide from apple induces apoptosis and cell cycle arrest in HT29 human colon cancer cells</title><author>Li, Qian ; Zhou, Siyuan ; Jing, Juan ; Yang, Tiehong ; Duan, Shuangyan ; Wang, Zhongfu ; Mei, Qibing ; Liu, Li</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-f7c525fdd126035d83827658bb3c1cd5624abef29825977f26a285fda94e0f463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>alkaline hydrolysis</topic><topic>antineoplastic agents</topic><topic>Antineoplastic Agents - chemistry</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>apples</topic><topic>bcl-X Protein - metabolism</topic><topic>Cell cycle arrest</topic><topic>cell cycle checkpoints</topic><topic>Cell Cycle Checkpoints - drug effects</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival - drug effects</topic><topic>cell viability</topic><topic>chemoprevention</topic><topic>chromatography</topic><topic>Colonic Neoplasms - drug therapy</topic><topic>Colonic Neoplasms - metabolism</topic><topic>Colonic Neoplasms - pathology</topic><topic>colorectal neoplasms</topic><topic>Cyclin B1 - metabolism</topic><topic>Cyclin-Dependent Kinase 2 - metabolism</topic><topic>cyclins</topic><topic>dose response</topic><topic>Humans</topic><topic>interphase</topic><topic>Malus - chemistry</topic><topic>neoplasm cells</topic><topic>Oligosaccharide</topic><topic>oligosaccharides</topic><topic>Oligosaccharides - chemistry</topic><topic>Oligosaccharides - pharmacology</topic><topic>polysaccharides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Qian</creatorcontrib><creatorcontrib>Zhou, Siyuan</creatorcontrib><creatorcontrib>Jing, Juan</creatorcontrib><creatorcontrib>Yang, Tiehong</creatorcontrib><creatorcontrib>Duan, Shuangyan</creatorcontrib><creatorcontrib>Wang, Zhongfu</creatorcontrib><creatorcontrib>Mei, Qibing</creatorcontrib><creatorcontrib>Liu, Li</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Qian</au><au>Zhou, Siyuan</au><au>Jing, Juan</au><au>Yang, Tiehong</au><au>Duan, Shuangyan</au><au>Wang, Zhongfu</au><au>Mei, Qibing</au><au>Liu, Li</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oligosaccharide from apple induces apoptosis and cell cycle arrest in HT29 human colon cancer cells</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2013-06-01</date><risdate>2013</risdate><volume>57</volume><spage>245</spage><epage>254</epage><pages>245-254</pages><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>It is reported that apple polysaccharide can prevent colon cancer growth and impede colon cancer progression. Apple oligosaccharide was prepared by the combination of alkaline hydrolysis and enzymolysis of apple polysaccharides, and purified by anion column chromatography. The aim of this study is to explore the effect of apple oligosaccharide on the cellular viability of human colon carcinoma cells (HT29 cells) and its mechanism. The results showed that apple oligosaccharide decreased the cellular viability of HT29 cells in dose-dependent manner. Meanwhile it enhanced the expression of Bax; and decreased the levels of Bcl-2 and Bcl-xl. Apple oligosaccharide induced cell cycle arrest in S phase, which correlated with the decreased expression of Cdk 2 and cyclin B1. These results indicated that apple oligosaccharide attenuated HT29 cell viability by inducing cell apoptosis and cell cycle arrest. Apple oligosaccharide is a potential chemoprevention agent or anti-tumor agent and is worthy of further study.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>23511050</pmid><doi>10.1016/j.ijbiomac.2013.03.034</doi><tpages>10</tpages></addata></record> |
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| subjects | alkaline hydrolysis antineoplastic agents Antineoplastic Agents - chemistry Antineoplastic Agents - pharmacology Apoptosis Apoptosis - drug effects apples bcl-X Protein - metabolism Cell cycle arrest cell cycle checkpoints Cell Cycle Checkpoints - drug effects Cell Line, Tumor Cell Survival - drug effects cell viability chemoprevention chromatography Colonic Neoplasms - drug therapy Colonic Neoplasms - metabolism Colonic Neoplasms - pathology colorectal neoplasms Cyclin B1 - metabolism Cyclin-Dependent Kinase 2 - metabolism cyclins dose response Humans interphase Malus - chemistry neoplasm cells Oligosaccharide oligosaccharides Oligosaccharides - chemistry Oligosaccharides - pharmacology polysaccharides |
| title | Oligosaccharide from apple induces apoptosis and cell cycle arrest in HT29 human colon cancer cells |
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