Selection and characterization of DNA aptamers for use in detection of avian influenza virus H5N1

•Three DNA aptamers specifically against avian influenza virus H5N1 were selected.•Kinetic analysis was conducted for the best aptamer using surface plasmon resonance.•Interaction between the selected aptamer and H5N1 virus was studied.•The developed aptamer was able to recognize target H5N1 in chic...

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Veröffentlicht in:	Journal of virological methods 2013-05, Vol.189 (2), p.362-369
Hauptverfasser:	Wang, Ronghui, Zhao, Jingjing, Jiang, Tieshan, Kwon, Young M., Lu, Huaguang, Jiao, Peirong, Liao, Ming, Li, Yanbin
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals antibodies Aptamers, Nucleotide - isolation & purification Avian influenza binding capacity color Detection diagnostic techniques dissociation DNA aptamer H5N1 Hemagglutinin Glycoproteins, Influenza Virus - analysis hemagglutinins Influenza A virus Influenza A Virus, H5N1 Subtype - genetics Influenza A Virus, H5N1 Subtype - isolation & purification Influenza in Birds - diagnosis Influenza in Birds - virology Kinetics monitoring oligonucleotides Poultry Protein Binding RNA SELEX Sensitivity and Specificity single-stranded DNA Surface Plasmon Resonance systematic evolution of ligands by exponential enrichment viruses
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