Modulation of calcium signalling by the endoplasmic reticulum in Carassius neurons
•We firstly show that the ER participates in Ca2+ signalling of Carassius neurons.•SERCA pump is activated immediately after the beginning of Ca2+ entry the cell.•The ER does not influence basal level of intracellular Ca2+ in Carassius neurons.•The ER modulates the shape of Ca2+ signals in cerebella...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2013-04, Vol.433 (4), p.591-594 |
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| creator | Lukyanets, Igor A. Lukyanetz, Elena A. |
| description | •We firstly show that the ER participates in Ca2+ signalling of Carassius neurons.•SERCA pump is activated immediately after the beginning of Ca2+ entry the cell.•The ER does not influence basal level of intracellular Ca2+ in Carassius neurons.•The ER modulates the shape of Ca2+ signals in cerebellar neurons.•The ER plays significant role in the short-term modulation of Ca2+ signals.
It is known that endoplasmic reticulum (ER), being a calcium store participates in the regulation of intracellular calcium concentration. Ca-ATPase of the ER is one of the crucial agents providing the calcium-accumulating function of this intracellular structure. We studied the role of the ER in modulation of calcium signalling in Carassius neurons using a Ca2+-imaging technique. We tested the role of the ER in the maintenance of a steady state calcium level in the cytoplasm and in modulation of Ca2+ transients evoked by cell depolarizations. The ER calcium stores were depleted using inhibitors of ER Ca-ATPase, which provided blocking of Ca2+ uptake by the ER. Our experiments firstly showed that the ER can significantly modulate the characteristics of intracellular calcium signals in Carassius neurons during their activity. These findings also indicate that the ER modulates the shape of Ca2+ signals rather than the basal level of intracellular Ca2+ in these neurons. |
| doi_str_mv | 10.1016/j.bbrc.2013.03.035 |
| format | Article |
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It is known that endoplasmic reticulum (ER), being a calcium store participates in the regulation of intracellular calcium concentration. Ca-ATPase of the ER is one of the crucial agents providing the calcium-accumulating function of this intracellular structure. We studied the role of the ER in modulation of calcium signalling in Carassius neurons using a Ca2+-imaging technique. We tested the role of the ER in the maintenance of a steady state calcium level in the cytoplasm and in modulation of Ca2+ transients evoked by cell depolarizations. The ER calcium stores were depleted using inhibitors of ER Ca-ATPase, which provided blocking of Ca2+ uptake by the ER. Our experiments firstly showed that the ER can significantly modulate the characteristics of intracellular calcium signals in Carassius neurons during their activity. These findings also indicate that the ER modulates the shape of Ca2+ signals rather than the basal level of intracellular Ca2+ in these neurons.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2013.03.035</identifier><identifier>PMID: 23523783</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Brain - metabolism ; Calcium ; Calcium - metabolism ; Calcium Signaling ; Carassius gibelio ; Carps - metabolism ; Cell Survival ; Cerebellar neurons ; Cytoplasm - metabolism ; Endoplasmic reticulum ; Endoplasmic Reticulum - metabolism ; Enzyme Activation ; Fish ; Fish Proteins - metabolism ; Fura-2 - metabolism ; Image Processing, Computer-Assisted ; Indoles - pharmacology ; Neurons - metabolism ; Potassium Chloride - pharmacology ; Reproducibility of Results ; Sarcoplasmic Reticulum Calcium-Transporting ATPases - antagonists & inhibitors ; Sarcoplasmic Reticulum Calcium-Transporting ATPases - metabolism ; SERCA ; Thapsigargin - pharmacology</subject><ispartof>Biochemical and biophysical research communications, 2013-04, Vol.433 (4), p.591-594</ispartof><rights>2013 Elsevier Inc.</rights><rights>Copyright © 2013 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-b33a7d097d6fbc38714854105c77230cbf8561394ed067a88a9f9b5b1e4d97bf3</citedby><cites>FETCH-LOGICAL-c356t-b33a7d097d6fbc38714854105c77230cbf8561394ed067a88a9f9b5b1e4d97bf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2013.03.035$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23523783$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lukyanets, Igor A.</creatorcontrib><creatorcontrib>Lukyanetz, Elena A.</creatorcontrib><title>Modulation of calcium signalling by the endoplasmic reticulum in Carassius neurons</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>•We firstly show that the ER participates in Ca2+ signalling of Carassius neurons.•SERCA pump is activated immediately after the beginning of Ca2+ entry the cell.•The ER does not influence basal level of intracellular Ca2+ in Carassius neurons.•The ER modulates the shape of Ca2+ signals in cerebellar neurons.•The ER plays significant role in the short-term modulation of Ca2+ signals.
It is known that endoplasmic reticulum (ER), being a calcium store participates in the regulation of intracellular calcium concentration. Ca-ATPase of the ER is one of the crucial agents providing the calcium-accumulating function of this intracellular structure. We studied the role of the ER in modulation of calcium signalling in Carassius neurons using a Ca2+-imaging technique. We tested the role of the ER in the maintenance of a steady state calcium level in the cytoplasm and in modulation of Ca2+ transients evoked by cell depolarizations. The ER calcium stores were depleted using inhibitors of ER Ca-ATPase, which provided blocking of Ca2+ uptake by the ER. Our experiments firstly showed that the ER can significantly modulate the characteristics of intracellular calcium signals in Carassius neurons during their activity. These findings also indicate that the ER modulates the shape of Ca2+ signals rather than the basal level of intracellular Ca2+ in these neurons.</description><subject>Animals</subject><subject>Brain - metabolism</subject><subject>Calcium</subject><subject>Calcium - metabolism</subject><subject>Calcium Signaling</subject><subject>Carassius gibelio</subject><subject>Carps - metabolism</subject><subject>Cell Survival</subject><subject>Cerebellar neurons</subject><subject>Cytoplasm - metabolism</subject><subject>Endoplasmic reticulum</subject><subject>Endoplasmic Reticulum - metabolism</subject><subject>Enzyme Activation</subject><subject>Fish</subject><subject>Fish Proteins - metabolism</subject><subject>Fura-2 - metabolism</subject><subject>Image Processing, Computer-Assisted</subject><subject>Indoles - pharmacology</subject><subject>Neurons - metabolism</subject><subject>Potassium Chloride - pharmacology</subject><subject>Reproducibility of Results</subject><subject>Sarcoplasmic Reticulum Calcium-Transporting ATPases - antagonists & inhibitors</subject><subject>Sarcoplasmic Reticulum Calcium-Transporting ATPases - metabolism</subject><subject>SERCA</subject><subject>Thapsigargin - pharmacology</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r3DAURUVpaaZJ_kAXRctuPH2ybMuCbsqQtIWEQEggO6GP51SDLE0ku5B_H5tJuixceJtzL7xDyGcGWwas-7bfGpPttgbGt7CmfUc2DCRUNYPmPdkAQFfVkj2ckE-l7AEYazr5kZzUvK256PmG3F4nNwc9-RRpGqjVwfp5pMU_Rh2Cj4_UPNPpD1KMLh2CLqO3NOPk7RwWzke601mX4udCI845xXJGPgw6FDx_vafk_vLibverurr5-Xv346qyvO2mynCuhQMpXDcYy3vBmr5tGLRWiJqDNUPfdozLBh10Qve9loM0rWHYOCnMwE_J1-PuIaenGcukRl8shqAjprkoxnnPJQjRLGh9RG1OpWQc1CH7UednxUCtLtVerS7V6lLBmnYpfXndn82I7l_lTd4CfD8CuHz512NWxXqMFp3PaCflkv_f_gsV7oWN</recordid><startdate>20130419</startdate><enddate>20130419</enddate><creator>Lukyanets, Igor A.</creator><creator>Lukyanetz, Elena A.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130419</creationdate><title>Modulation of calcium signalling by the endoplasmic reticulum in Carassius neurons</title><author>Lukyanets, Igor A. ; Lukyanetz, Elena A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-b33a7d097d6fbc38714854105c77230cbf8561394ed067a88a9f9b5b1e4d97bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Brain - metabolism</topic><topic>Calcium</topic><topic>Calcium - metabolism</topic><topic>Calcium Signaling</topic><topic>Carassius gibelio</topic><topic>Carps - metabolism</topic><topic>Cell Survival</topic><topic>Cerebellar neurons</topic><topic>Cytoplasm - metabolism</topic><topic>Endoplasmic reticulum</topic><topic>Endoplasmic Reticulum - metabolism</topic><topic>Enzyme Activation</topic><topic>Fish</topic><topic>Fish Proteins - metabolism</topic><topic>Fura-2 - metabolism</topic><topic>Image Processing, Computer-Assisted</topic><topic>Indoles - pharmacology</topic><topic>Neurons - metabolism</topic><topic>Potassium Chloride - pharmacology</topic><topic>Reproducibility of Results</topic><topic>Sarcoplasmic Reticulum Calcium-Transporting ATPases - antagonists & inhibitors</topic><topic>Sarcoplasmic Reticulum Calcium-Transporting ATPases - metabolism</topic><topic>SERCA</topic><topic>Thapsigargin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lukyanets, Igor A.</creatorcontrib><creatorcontrib>Lukyanetz, Elena A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lukyanets, Igor A.</au><au>Lukyanetz, Elena A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulation of calcium signalling by the endoplasmic reticulum in Carassius neurons</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2013-04-19</date><risdate>2013</risdate><volume>433</volume><issue>4</issue><spage>591</spage><epage>594</epage><pages>591-594</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>•We firstly show that the ER participates in Ca2+ signalling of Carassius neurons.•SERCA pump is activated immediately after the beginning of Ca2+ entry the cell.•The ER does not influence basal level of intracellular Ca2+ in Carassius neurons.•The ER modulates the shape of Ca2+ signals in cerebellar neurons.•The ER plays significant role in the short-term modulation of Ca2+ signals.
It is known that endoplasmic reticulum (ER), being a calcium store participates in the regulation of intracellular calcium concentration. Ca-ATPase of the ER is one of the crucial agents providing the calcium-accumulating function of this intracellular structure. We studied the role of the ER in modulation of calcium signalling in Carassius neurons using a Ca2+-imaging technique. We tested the role of the ER in the maintenance of a steady state calcium level in the cytoplasm and in modulation of Ca2+ transients evoked by cell depolarizations. The ER calcium stores were depleted using inhibitors of ER Ca-ATPase, which provided blocking of Ca2+ uptake by the ER. Our experiments firstly showed that the ER can significantly modulate the characteristics of intracellular calcium signals in Carassius neurons during their activity. These findings also indicate that the ER modulates the shape of Ca2+ signals rather than the basal level of intracellular Ca2+ in these neurons.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>23523783</pmid><doi>10.1016/j.bbrc.2013.03.035</doi><tpages>4</tpages></addata></record> |
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| subjects | Animals Brain - metabolism Calcium Calcium - metabolism Calcium Signaling Carassius gibelio Carps - metabolism Cell Survival Cerebellar neurons Cytoplasm - metabolism Endoplasmic reticulum Endoplasmic Reticulum - metabolism Enzyme Activation Fish Fish Proteins - metabolism Fura-2 - metabolism Image Processing, Computer-Assisted Indoles - pharmacology Neurons - metabolism Potassium Chloride - pharmacology Reproducibility of Results Sarcoplasmic Reticulum Calcium-Transporting ATPases - antagonists & inhibitors Sarcoplasmic Reticulum Calcium-Transporting ATPases - metabolism SERCA Thapsigargin - pharmacology |
| title | Modulation of calcium signalling by the endoplasmic reticulum in Carassius neurons |
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