In vitro antimicrobial and anticancer potential of hinokitiol against oral pathogens and oral cancer cell lines
Hinokitiol is a natural component isolated from Chamacyparis taiwanensis. It has anti-microbial activity, and has been used in oral care products. The minimal inhibitory concentration (MIC) and minimal microbicidal concentration (MMC) of hinokitiol against MRSA, Aggregatibacter actinomycetemcomitans...
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| Veröffentlicht in: | Microbiological research 2013-06, Vol.168 (5), p.254-262 |
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| creator | Shih, Yin-Hua Chang, Kuo-Wei Hsia, Shih-Min Yu, Cheng-Chia Fuh, Lih-Jyh Chi, Tzu-Yun Shieh, Tzong-Ming |
| description | Hinokitiol is a natural component isolated from Chamacyparis taiwanensis. It has anti-microbial activity, and has been used in oral care products. The minimal inhibitory concentration (MIC) and minimal microbicidal concentration (MMC) of hinokitiol against MRSA, Aggregatibacter actinomycetemcomitans, Streptococcus mutans, and Candida albicans were determined by the agar and broth dilution method (MIC: 40–110μM; MMC: 50–130μM); the paradoxical inhibition phenomenon (PIP) was observed in A. actinomycetemcomitans and S. mutans. The PIP can be described as microbial growth occurring in the presence of both high and low concentrations of a compound, between which microbial growth is inhibited. The PIP was confirmed using a kinetic microplate and inhibition zone methods. The PIP was also observed in MRSA. The low autolysin activity somehow correlated to the PIP positive. The cell diameter was increased in all the pathogens, and the transition was inhibited in C. albicans following hinokitiol treatment. Hinokitiol is also a potential anticancer drug. The 200μM of hinokitiol has significant antimicrobial and cytotoxic activities against oral pathogens and oral squamous cell carcinoma cell lines, respectively, and lower cytotoxic effects for normal human oral keratinocytes, indicating that hinokitiol displays a high potential for safe and effective applications in oral health care. |
| doi_str_mv | 10.1016/j.micres.2012.12.007 |
| format | Article |
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It has anti-microbial activity, and has been used in oral care products. The minimal inhibitory concentration (MIC) and minimal microbicidal concentration (MMC) of hinokitiol against MRSA, Aggregatibacter actinomycetemcomitans, Streptococcus mutans, and Candida albicans were determined by the agar and broth dilution method (MIC: 40–110μM; MMC: 50–130μM); the paradoxical inhibition phenomenon (PIP) was observed in A. actinomycetemcomitans and S. mutans. The PIP can be described as microbial growth occurring in the presence of both high and low concentrations of a compound, between which microbial growth is inhibited. The PIP was confirmed using a kinetic microplate and inhibition zone methods. The PIP was also observed in MRSA. The low autolysin activity somehow correlated to the PIP positive. The cell diameter was increased in all the pathogens, and the transition was inhibited in C. albicans following hinokitiol treatment. Hinokitiol is also a potential anticancer drug. The 200μM of hinokitiol has significant antimicrobial and cytotoxic activities against oral pathogens and oral squamous cell carcinoma cell lines, respectively, and lower cytotoxic effects for normal human oral keratinocytes, indicating that hinokitiol displays a high potential for safe and effective applications in oral health care.</description><identifier>ISSN: 0944-5013</identifier><identifier>EISSN: 1618-0623</identifier><identifier>DOI: 10.1016/j.micres.2012.12.007</identifier><identifier>PMID: 23312825</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Actinobacillus actinomycetemcomitans ; agar ; Anti-Bacterial Agents - pharmacology ; anti-infective properties ; antineoplastic agents ; Antineoplastic Agents - pharmacology ; Autolysin ; Bacteria - drug effects ; Candida albicans ; Candida albicans - drug effects ; Cell Line, Tumor ; Cell Survival - drug effects ; cytotoxicity ; Drug Screening Assays, Antitumor ; gametolysin ; health services ; Hinokitiol ; Humans ; keratinocytes ; microbial growth ; Microbial Sensitivity Tests ; Microbial Viability - drug effects ; Minimal inhibitory concentrations ; Minimal microbicidal concentration ; minimum inhibitory concentration ; Monoterpenes - pharmacology ; mouth neoplasms ; neoplasm cells ; Oral squamous cell carcinoma ; Paradoxical inhibition phenomenon (PIP) ; pathogens ; squamous cell carcinoma ; Streptococcus mutans ; Tropolone - analogs & derivatives ; Tropolone - pharmacology</subject><ispartof>Microbiological research, 2013-06, Vol.168 (5), p.254-262</ispartof><rights>2012 Elsevier GmbH</rights><rights>Copyright © 2012 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-3cdddd1120bed9d5f77002eb3356d4843c9eef98435bf8bc6473cd6225543a303</citedby><cites>FETCH-LOGICAL-c452t-3cdddd1120bed9d5f77002eb3356d4843c9eef98435bf8bc6473cd6225543a303</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.micres.2012.12.007$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23312825$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shih, Yin-Hua</creatorcontrib><creatorcontrib>Chang, Kuo-Wei</creatorcontrib><creatorcontrib>Hsia, Shih-Min</creatorcontrib><creatorcontrib>Yu, Cheng-Chia</creatorcontrib><creatorcontrib>Fuh, Lih-Jyh</creatorcontrib><creatorcontrib>Chi, Tzu-Yun</creatorcontrib><creatorcontrib>Shieh, Tzong-Ming</creatorcontrib><title>In vitro antimicrobial and anticancer potential of hinokitiol against oral pathogens and oral cancer cell lines</title><title>Microbiological research</title><addtitle>Microbiol Res</addtitle><description>Hinokitiol is a natural component isolated from Chamacyparis taiwanensis. It has anti-microbial activity, and has been used in oral care products. The minimal inhibitory concentration (MIC) and minimal microbicidal concentration (MMC) of hinokitiol against MRSA, Aggregatibacter actinomycetemcomitans, Streptococcus mutans, and Candida albicans were determined by the agar and broth dilution method (MIC: 40–110μM; MMC: 50–130μM); the paradoxical inhibition phenomenon (PIP) was observed in A. actinomycetemcomitans and S. mutans. The PIP can be described as microbial growth occurring in the presence of both high and low concentrations of a compound, between which microbial growth is inhibited. The PIP was confirmed using a kinetic microplate and inhibition zone methods. The PIP was also observed in MRSA. The low autolysin activity somehow correlated to the PIP positive. The cell diameter was increased in all the pathogens, and the transition was inhibited in C. albicans following hinokitiol treatment. Hinokitiol is also a potential anticancer drug. The 200μM of hinokitiol has significant antimicrobial and cytotoxic activities against oral pathogens and oral squamous cell carcinoma cell lines, respectively, and lower cytotoxic effects for normal human oral keratinocytes, indicating that hinokitiol displays a high potential for safe and effective applications in oral health care.</description><subject>Actinobacillus actinomycetemcomitans</subject><subject>agar</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>anti-infective properties</subject><subject>antineoplastic agents</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Autolysin</subject><subject>Bacteria - drug effects</subject><subject>Candida albicans</subject><subject>Candida albicans - drug effects</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival - drug effects</subject><subject>cytotoxicity</subject><subject>Drug Screening Assays, Antitumor</subject><subject>gametolysin</subject><subject>health services</subject><subject>Hinokitiol</subject><subject>Humans</subject><subject>keratinocytes</subject><subject>microbial growth</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbial Viability - drug effects</subject><subject>Minimal inhibitory concentrations</subject><subject>Minimal microbicidal concentration</subject><subject>minimum inhibitory concentration</subject><subject>Monoterpenes - pharmacology</subject><subject>mouth neoplasms</subject><subject>neoplasm cells</subject><subject>Oral squamous cell carcinoma</subject><subject>Paradoxical inhibition phenomenon (PIP)</subject><subject>pathogens</subject><subject>squamous cell carcinoma</subject><subject>Streptococcus mutans</subject><subject>Tropolone - analogs & derivatives</subject><subject>Tropolone - pharmacology</subject><issn>0944-5013</issn><issn>1618-0623</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1PGzEQhq0KBCnlH1Swx142jL_241IJoX4gIfVAOVte72xw2KxT20Hqv2eSDT3WsmTPzPuO7ceMfeaw5MCrm_Vy413EtBTAxZImQP2BLXjFmxIqIU_YAlqlSg1cnrOPKa0BuGobccbOhZRcNEIvWLifilefYyjslP2-Y-i8HSnqDxlnJ4ex2IaMFFEhDMWzn8KLzz6QbGX9lHIRIpW2Nj-HFU7p4D6kjnaH41iMfsL0iZ0Odkx4eVwv2NP3b7_vfpYPv37c390-lE5pkUvpehqcC-iwb3s91DWAwE5KXfWqUdK1iENLG90NTecqVZOlEkJrJa0EecG-zH23MfzZYcpm49P-GnbCsEuGEwFo2gYkSdUspbenFHEw2-g3Nv41HMwetVmbGbXZozY0CTXZro4n7LoN9v9M72xJcD0LBhuMXUWfzNMjdajoHwSvdUOKr7MCicSrx2iS80jEeh_RZdMH__87vAEX6pv5</recordid><startdate>20130612</startdate><enddate>20130612</enddate><creator>Shih, Yin-Hua</creator><creator>Chang, Kuo-Wei</creator><creator>Hsia, Shih-Min</creator><creator>Yu, Cheng-Chia</creator><creator>Fuh, Lih-Jyh</creator><creator>Chi, Tzu-Yun</creator><creator>Shieh, Tzong-Ming</creator><general>Elsevier GmbH</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130612</creationdate><title>In vitro antimicrobial and anticancer potential of hinokitiol against oral pathogens and oral cancer cell lines</title><author>Shih, Yin-Hua ; Chang, Kuo-Wei ; Hsia, Shih-Min ; Yu, Cheng-Chia ; Fuh, Lih-Jyh ; Chi, Tzu-Yun ; Shieh, Tzong-Ming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-3cdddd1120bed9d5f77002eb3356d4843c9eef98435bf8bc6473cd6225543a303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Actinobacillus actinomycetemcomitans</topic><topic>agar</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>anti-infective properties</topic><topic>antineoplastic agents</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Autolysin</topic><topic>Bacteria - drug effects</topic><topic>Candida albicans</topic><topic>Candida albicans - drug effects</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival - drug effects</topic><topic>cytotoxicity</topic><topic>Drug Screening Assays, Antitumor</topic><topic>gametolysin</topic><topic>health services</topic><topic>Hinokitiol</topic><topic>Humans</topic><topic>keratinocytes</topic><topic>microbial growth</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbial Viability - drug effects</topic><topic>Minimal inhibitory concentrations</topic><topic>Minimal microbicidal concentration</topic><topic>minimum inhibitory concentration</topic><topic>Monoterpenes - pharmacology</topic><topic>mouth neoplasms</topic><topic>neoplasm cells</topic><topic>Oral squamous cell carcinoma</topic><topic>Paradoxical inhibition phenomenon (PIP)</topic><topic>pathogens</topic><topic>squamous cell carcinoma</topic><topic>Streptococcus mutans</topic><topic>Tropolone - analogs & derivatives</topic><topic>Tropolone - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shih, Yin-Hua</creatorcontrib><creatorcontrib>Chang, Kuo-Wei</creatorcontrib><creatorcontrib>Hsia, Shih-Min</creatorcontrib><creatorcontrib>Yu, Cheng-Chia</creatorcontrib><creatorcontrib>Fuh, Lih-Jyh</creatorcontrib><creatorcontrib>Chi, Tzu-Yun</creatorcontrib><creatorcontrib>Shieh, Tzong-Ming</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microbiological research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shih, Yin-Hua</au><au>Chang, Kuo-Wei</au><au>Hsia, Shih-Min</au><au>Yu, Cheng-Chia</au><au>Fuh, Lih-Jyh</au><au>Chi, Tzu-Yun</au><au>Shieh, Tzong-Ming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro antimicrobial and anticancer potential of hinokitiol against oral pathogens and oral cancer cell lines</atitle><jtitle>Microbiological research</jtitle><addtitle>Microbiol Res</addtitle><date>2013-06-12</date><risdate>2013</risdate><volume>168</volume><issue>5</issue><spage>254</spage><epage>262</epage><pages>254-262</pages><issn>0944-5013</issn><eissn>1618-0623</eissn><abstract>Hinokitiol is a natural component isolated from Chamacyparis taiwanensis. It has anti-microbial activity, and has been used in oral care products. The minimal inhibitory concentration (MIC) and minimal microbicidal concentration (MMC) of hinokitiol against MRSA, Aggregatibacter actinomycetemcomitans, Streptococcus mutans, and Candida albicans were determined by the agar and broth dilution method (MIC: 40–110μM; MMC: 50–130μM); the paradoxical inhibition phenomenon (PIP) was observed in A. actinomycetemcomitans and S. mutans. The PIP can be described as microbial growth occurring in the presence of both high and low concentrations of a compound, between which microbial growth is inhibited. The PIP was confirmed using a kinetic microplate and inhibition zone methods. The PIP was also observed in MRSA. The low autolysin activity somehow correlated to the PIP positive. The cell diameter was increased in all the pathogens, and the transition was inhibited in C. albicans following hinokitiol treatment. Hinokitiol is also a potential anticancer drug. The 200μM of hinokitiol has significant antimicrobial and cytotoxic activities against oral pathogens and oral squamous cell carcinoma cell lines, respectively, and lower cytotoxic effects for normal human oral keratinocytes, indicating that hinokitiol displays a high potential for safe and effective applications in oral health care.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>23312825</pmid><doi>10.1016/j.micres.2012.12.007</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Microbiological research, 2013-06, Vol.168 (5), p.254-262 |
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| language | eng |
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| subjects | Actinobacillus actinomycetemcomitans agar Anti-Bacterial Agents - pharmacology anti-infective properties antineoplastic agents Antineoplastic Agents - pharmacology Autolysin Bacteria - drug effects Candida albicans Candida albicans - drug effects Cell Line, Tumor Cell Survival - drug effects cytotoxicity Drug Screening Assays, Antitumor gametolysin health services Hinokitiol Humans keratinocytes microbial growth Microbial Sensitivity Tests Microbial Viability - drug effects Minimal inhibitory concentrations Minimal microbicidal concentration minimum inhibitory concentration Monoterpenes - pharmacology mouth neoplasms neoplasm cells Oral squamous cell carcinoma Paradoxical inhibition phenomenon (PIP) pathogens squamous cell carcinoma Streptococcus mutans Tropolone - analogs & derivatives Tropolone - pharmacology |
| title | In vitro antimicrobial and anticancer potential of hinokitiol against oral pathogens and oral cancer cell lines |
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