Transcriptional regulation of human ferredoxin 1 in ovarian granulosa cells
► cAMP stimulation rapidly increases the expression of FDX1 in the rat ovarian granulosa cells. ► In differentiated hMSCs, SF-1 binds to the promoter of FDX1. ► Two SF-1 binding sites and a CRE-like sequence affect the FDX1 promoter activity. ► NR5A family and cAMP synergistically activate transcrip...
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| Veröffentlicht in: | Molecular and cellular endocrinology 2013-05, Vol.370 (1-2), p.1-10 |
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| creator | Imamichi, Yoshitaka Mizutani, Tetsuya Ju, Yunfeng Matsumura, Takehiro Kawabe, Shinya Kanno, Masafumi Yazawa, Takashi Miyamoto, Kaoru |
| description | ► cAMP stimulation rapidly increases the expression of FDX1 in the rat ovarian granulosa cells. ► In differentiated hMSCs, SF-1 binds to the promoter of FDX1. ► Two SF-1 binding sites and a CRE-like sequence affect the FDX1 promoter activity. ► NR5A family and cAMP synergistically activate transcription from the FDX1 promoter.
Ferredoxin 1 (FDX1; adrenodoxin) is an iron–sulfur protein that is involved in various metabolic processes, including steroid hormone synthesis in mammalian tissues. We investigated the transcriptional regulation of FDX1 in ovarian granulosa cells. Previously, we reported that the NR5A family, including steroidogenic factor-1 (SF-1) and liver receptor homolog-1 could induce differentiation of human mesenchymal stem cells (hMSCs) into steroidogenic cells. A ChIP assay showed that SF-1 could bind to the FDX1 promoter in differentiated hMSCs. Luciferase reporter assays showed that transcription of FDX1 was synergistically activated by the NR5A family and 8Br-cAMP treatment through two SF-1 binding sites and a CRE-like sequence in a human ovarian granulosa cell line, KGN. Knockdown of FDX1 attenuated progesterone production in KGN cells. These results indicate transcription of FDX1 is regulated by the NR5A family and cAMP signaling, and participates in steroid hormone production in ovarian granulosa cells. |
| doi_str_mv | 10.1016/j.mce.2013.02.012 |
| format | Article |
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Ferredoxin 1 (FDX1; adrenodoxin) is an iron–sulfur protein that is involved in various metabolic processes, including steroid hormone synthesis in mammalian tissues. We investigated the transcriptional regulation of FDX1 in ovarian granulosa cells. Previously, we reported that the NR5A family, including steroidogenic factor-1 (SF-1) and liver receptor homolog-1 could induce differentiation of human mesenchymal stem cells (hMSCs) into steroidogenic cells. A ChIP assay showed that SF-1 could bind to the FDX1 promoter in differentiated hMSCs. Luciferase reporter assays showed that transcription of FDX1 was synergistically activated by the NR5A family and 8Br-cAMP treatment through two SF-1 binding sites and a CRE-like sequence in a human ovarian granulosa cell line, KGN. Knockdown of FDX1 attenuated progesterone production in KGN cells. These results indicate transcription of FDX1 is regulated by the NR5A family and cAMP signaling, and participates in steroid hormone production in ovarian granulosa cells.</description><identifier>ISSN: 0303-7207</identifier><identifier>EISSN: 1872-8057</identifier><identifier>DOI: 10.1016/j.mce.2013.02.012</identifier><identifier>PMID: 23435367</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>8-Bromo Cyclic Adenosine Monophosphate - pharmacology ; Adrenodoxin - genetics ; Animals ; binding sites ; Cell Differentiation ; Cell Line, Tumor ; Cyclic AMP - metabolism ; DNA-Binding Proteins - metabolism ; Female ; Ferredoxin 1 ; Ferredoxins - biosynthesis ; Ferredoxins - genetics ; Gene Expression Regulation ; granulosa cells ; Granulosa Cells - metabolism ; HeLa Cells ; Humans ; liver ; Liver receptor homolog-1 ; luciferase ; Mesenchymal Stromal Cells - metabolism ; Ovarian granulosa cells ; progesterone ; Progesterone - biosynthesis ; Promoter Regions, Genetic ; Protein Binding ; Rats ; Rats, Wistar ; Receptors, Cytoplasmic and Nuclear - metabolism ; RNA Interference ; RNA, Small Interfering ; Signal Transduction ; stem cells ; steroid hormones ; Steroidogenesis ; Steroidogenic Factor 1 - drug effects ; Steroidogenic Factor 1 - metabolism ; Steroidogenic factor-1 ; tissues ; transcription (genetics) ; Transcription, Genetic ; Transcriptional regulation</subject><ispartof>Molecular and cellular endocrinology, 2013-05, Vol.370 (1-2), p.1-10</ispartof><rights>2013 Elsevier Ireland Ltd</rights><rights>Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-811b52b3d43315bebfb34773beb57431080fadf4b4acf8deee708d9fa94120d53</citedby><cites>FETCH-LOGICAL-c443t-811b52b3d43315bebfb34773beb57431080fadf4b4acf8deee708d9fa94120d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0303720713000701$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23435367$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Imamichi, Yoshitaka</creatorcontrib><creatorcontrib>Mizutani, Tetsuya</creatorcontrib><creatorcontrib>Ju, Yunfeng</creatorcontrib><creatorcontrib>Matsumura, Takehiro</creatorcontrib><creatorcontrib>Kawabe, Shinya</creatorcontrib><creatorcontrib>Kanno, Masafumi</creatorcontrib><creatorcontrib>Yazawa, Takashi</creatorcontrib><creatorcontrib>Miyamoto, Kaoru</creatorcontrib><title>Transcriptional regulation of human ferredoxin 1 in ovarian granulosa cells</title><title>Molecular and cellular endocrinology</title><addtitle>Mol Cell Endocrinol</addtitle><description>► cAMP stimulation rapidly increases the expression of FDX1 in the rat ovarian granulosa cells. ► In differentiated hMSCs, SF-1 binds to the promoter of FDX1. ► Two SF-1 binding sites and a CRE-like sequence affect the FDX1 promoter activity. ► NR5A family and cAMP synergistically activate transcription from the FDX1 promoter.
Ferredoxin 1 (FDX1; adrenodoxin) is an iron–sulfur protein that is involved in various metabolic processes, including steroid hormone synthesis in mammalian tissues. We investigated the transcriptional regulation of FDX1 in ovarian granulosa cells. Previously, we reported that the NR5A family, including steroidogenic factor-1 (SF-1) and liver receptor homolog-1 could induce differentiation of human mesenchymal stem cells (hMSCs) into steroidogenic cells. A ChIP assay showed that SF-1 could bind to the FDX1 promoter in differentiated hMSCs. Luciferase reporter assays showed that transcription of FDX1 was synergistically activated by the NR5A family and 8Br-cAMP treatment through two SF-1 binding sites and a CRE-like sequence in a human ovarian granulosa cell line, KGN. Knockdown of FDX1 attenuated progesterone production in KGN cells. These results indicate transcription of FDX1 is regulated by the NR5A family and cAMP signaling, and participates in steroid hormone production in ovarian granulosa cells.</description><subject>8-Bromo Cyclic Adenosine Monophosphate - pharmacology</subject><subject>Adrenodoxin - genetics</subject><subject>Animals</subject><subject>binding sites</subject><subject>Cell Differentiation</subject><subject>Cell Line, Tumor</subject><subject>Cyclic AMP - metabolism</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Female</subject><subject>Ferredoxin 1</subject><subject>Ferredoxins - biosynthesis</subject><subject>Ferredoxins - genetics</subject><subject>Gene Expression Regulation</subject><subject>granulosa cells</subject><subject>Granulosa Cells - metabolism</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>liver</subject><subject>Liver receptor homolog-1</subject><subject>luciferase</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Ovarian granulosa cells</subject><subject>progesterone</subject><subject>Progesterone - biosynthesis</subject><subject>Promoter Regions, Genetic</subject><subject>Protein Binding</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptors, Cytoplasmic and Nuclear - metabolism</subject><subject>RNA Interference</subject><subject>RNA, Small Interfering</subject><subject>Signal Transduction</subject><subject>stem cells</subject><subject>steroid hormones</subject><subject>Steroidogenesis</subject><subject>Steroidogenic Factor 1 - drug effects</subject><subject>Steroidogenic Factor 1 - metabolism</subject><subject>Steroidogenic factor-1</subject><subject>tissues</subject><subject>transcription (genetics)</subject><subject>Transcription, Genetic</subject><subject>Transcriptional regulation</subject><issn>0303-7207</issn><issn>1872-8057</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1O3DAUha2qVZkCD8CmzZJNwvUf9qirCtEfgdQFsLYc-3rwKIkHe4Lat8fRQJfd2L7Wd46uPkLOKHQU6OXFthsddgwo74B1QNk7sqJasVaDVO_JCjjwVjFQR-RTKVsAUJLpj-SIccElv1QrcnOf7VRcjrt9TJMdmoybebDL0KTQPM6jnZqAOaNPf-LU0KYe6dnmWP83NTsPqdjG4TCUE_Ih2KHg6et9TB6-X99f_Wxvf__4dfXttnVC8H2rKe0l67kXnFPZYx96LpTi9SWV4BQ0BOuD6IV1QXtEVKD9Oti1oAy85Mfk_NC7y-lpxrI3YyzLBnbCNBdD-VKi13pdUXpAXU6lZAxml-No819DwSwOzdZUh2ZxaICZ6rBmPr_Wz_2I_l_iTVoFvhyAYJOxmxyLebirDRKAasokVOLrgcCq4TliNsVFnBz6mNHtjU_xPwu8AA7Bi0o</recordid><startdate>20130506</startdate><enddate>20130506</enddate><creator>Imamichi, Yoshitaka</creator><creator>Mizutani, Tetsuya</creator><creator>Ju, Yunfeng</creator><creator>Matsumura, Takehiro</creator><creator>Kawabe, Shinya</creator><creator>Kanno, Masafumi</creator><creator>Yazawa, Takashi</creator><creator>Miyamoto, Kaoru</creator><general>Elsevier Ireland Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130506</creationdate><title>Transcriptional regulation of human ferredoxin 1 in ovarian granulosa cells</title><author>Imamichi, Yoshitaka ; Mizutani, Tetsuya ; Ju, Yunfeng ; Matsumura, Takehiro ; Kawabe, Shinya ; Kanno, Masafumi ; Yazawa, Takashi ; Miyamoto, Kaoru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-811b52b3d43315bebfb34773beb57431080fadf4b4acf8deee708d9fa94120d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>8-Bromo Cyclic Adenosine Monophosphate - pharmacology</topic><topic>Adrenodoxin - genetics</topic><topic>Animals</topic><topic>binding sites</topic><topic>Cell Differentiation</topic><topic>Cell Line, Tumor</topic><topic>Cyclic AMP - metabolism</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Female</topic><topic>Ferredoxin 1</topic><topic>Ferredoxins - biosynthesis</topic><topic>Ferredoxins - genetics</topic><topic>Gene Expression Regulation</topic><topic>granulosa cells</topic><topic>Granulosa Cells - metabolism</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>liver</topic><topic>Liver receptor homolog-1</topic><topic>luciferase</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>Ovarian granulosa cells</topic><topic>progesterone</topic><topic>Progesterone - biosynthesis</topic><topic>Promoter Regions, Genetic</topic><topic>Protein Binding</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptors, Cytoplasmic and Nuclear - metabolism</topic><topic>RNA Interference</topic><topic>RNA, Small Interfering</topic><topic>Signal Transduction</topic><topic>stem cells</topic><topic>steroid hormones</topic><topic>Steroidogenesis</topic><topic>Steroidogenic Factor 1 - drug effects</topic><topic>Steroidogenic Factor 1 - metabolism</topic><topic>Steroidogenic factor-1</topic><topic>tissues</topic><topic>transcription (genetics)</topic><topic>Transcription, Genetic</topic><topic>Transcriptional regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Imamichi, Yoshitaka</creatorcontrib><creatorcontrib>Mizutani, Tetsuya</creatorcontrib><creatorcontrib>Ju, Yunfeng</creatorcontrib><creatorcontrib>Matsumura, Takehiro</creatorcontrib><creatorcontrib>Kawabe, Shinya</creatorcontrib><creatorcontrib>Kanno, Masafumi</creatorcontrib><creatorcontrib>Yazawa, Takashi</creatorcontrib><creatorcontrib>Miyamoto, Kaoru</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Imamichi, Yoshitaka</au><au>Mizutani, Tetsuya</au><au>Ju, Yunfeng</au><au>Matsumura, Takehiro</au><au>Kawabe, Shinya</au><au>Kanno, Masafumi</au><au>Yazawa, Takashi</au><au>Miyamoto, Kaoru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptional regulation of human ferredoxin 1 in ovarian granulosa cells</atitle><jtitle>Molecular and cellular endocrinology</jtitle><addtitle>Mol Cell Endocrinol</addtitle><date>2013-05-06</date><risdate>2013</risdate><volume>370</volume><issue>1-2</issue><spage>1</spage><epage>10</epage><pages>1-10</pages><issn>0303-7207</issn><eissn>1872-8057</eissn><abstract>► cAMP stimulation rapidly increases the expression of FDX1 in the rat ovarian granulosa cells. ► In differentiated hMSCs, SF-1 binds to the promoter of FDX1. ► Two SF-1 binding sites and a CRE-like sequence affect the FDX1 promoter activity. ► NR5A family and cAMP synergistically activate transcription from the FDX1 promoter.
Ferredoxin 1 (FDX1; adrenodoxin) is an iron–sulfur protein that is involved in various metabolic processes, including steroid hormone synthesis in mammalian tissues. We investigated the transcriptional regulation of FDX1 in ovarian granulosa cells. Previously, we reported that the NR5A family, including steroidogenic factor-1 (SF-1) and liver receptor homolog-1 could induce differentiation of human mesenchymal stem cells (hMSCs) into steroidogenic cells. A ChIP assay showed that SF-1 could bind to the FDX1 promoter in differentiated hMSCs. Luciferase reporter assays showed that transcription of FDX1 was synergistically activated by the NR5A family and 8Br-cAMP treatment through two SF-1 binding sites and a CRE-like sequence in a human ovarian granulosa cell line, KGN. Knockdown of FDX1 attenuated progesterone production in KGN cells. These results indicate transcription of FDX1 is regulated by the NR5A family and cAMP signaling, and participates in steroid hormone production in ovarian granulosa cells.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>23435367</pmid><doi>10.1016/j.mce.2013.02.012</doi><tpages>10</tpages></addata></record> |
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| subjects | 8-Bromo Cyclic Adenosine Monophosphate - pharmacology Adrenodoxin - genetics Animals binding sites Cell Differentiation Cell Line, Tumor Cyclic AMP - metabolism DNA-Binding Proteins - metabolism Female Ferredoxin 1 Ferredoxins - biosynthesis Ferredoxins - genetics Gene Expression Regulation granulosa cells Granulosa Cells - metabolism HeLa Cells Humans liver Liver receptor homolog-1 luciferase Mesenchymal Stromal Cells - metabolism Ovarian granulosa cells progesterone Progesterone - biosynthesis Promoter Regions, Genetic Protein Binding Rats Rats, Wistar Receptors, Cytoplasmic and Nuclear - metabolism RNA Interference RNA, Small Interfering Signal Transduction stem cells steroid hormones Steroidogenesis Steroidogenic Factor 1 - drug effects Steroidogenic Factor 1 - metabolism Steroidogenic factor-1 tissues transcription (genetics) Transcription, Genetic Transcriptional regulation |
| title | Transcriptional regulation of human ferredoxin 1 in ovarian granulosa cells |
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