Antenatal ureaplasma infection impairs development of the fetal ovine gut in an IL-1-dependent manner
Ureaplasma infection of the amniotic cavity is associated with adverse postnatal intestinal outcomes. We tested whether interleukin-1 (IL-1) signaling underlies intestinal pathology following ureaplasma exposure in fetal sheep. Pregnant ewes received intra-amniotic injections of ureaplasma or cultur...
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| Veröffentlicht in: | Mucosal immunology 2013-05, Vol.6 (3), p.547-556 |
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| creator | Wolfs, T G A M Kallapur, S G Knox, C L Thuijls, G Nitsos, I Polglase, G R Collins, J J P Kroon, E Spierings, J Shroyer, N F Newnham, J P Jobe, A H Kramer, B W |
| description | Ureaplasma infection of the amniotic cavity is associated with adverse postnatal intestinal outcomes. We tested whether interleukin-1 (IL-1) signaling underlies intestinal pathology following ureaplasma exposure in fetal sheep. Pregnant ewes received intra-amniotic injections of ureaplasma or culture media for controls at 3, 7, and 14 d before preterm delivery at 124 d gestation (term 150 d). Intra-amniotic injections of recombinant human interleukin IL-1 receptor antagonist (rhIL-1ra) or saline for controls were given 3 h before and every 2 d after Ureaplasma injection. Ureaplasma exposure caused fetal gut inflammation within 7 d with damaged villus epithelium and gut barrier loss. Proliferation, differentiation, and maturation of enterocytes were significantly reduced after 7 d of ureaplasma exposure, leading to severe villus atrophy at 14 d. Inflammation, impaired development and villus atrophy of the fetal gut was largely prevented by intra-uterine rhIL-1ra treatment. These data form the basis for a clinical understanding of the role of ureaplasma in postnatal intestinal pathologies. |
| doi_str_mv | 10.1038/mi.2012.97 |
| format | Article |
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We tested whether interleukin-1 (IL-1) signaling underlies intestinal pathology following ureaplasma exposure in fetal sheep. Pregnant ewes received intra-amniotic injections of ureaplasma or culture media for controls at 3, 7, and 14 d before preterm delivery at 124 d gestation (term 150 d). Intra-amniotic injections of recombinant human interleukin IL-1 receptor antagonist (rhIL-1ra) or saline for controls were given 3 h before and every 2 d after Ureaplasma injection. Ureaplasma exposure caused fetal gut inflammation within 7 d with damaged villus epithelium and gut barrier loss. Proliferation, differentiation, and maturation of enterocytes were significantly reduced after 7 d of ureaplasma exposure, leading to severe villus atrophy at 14 d. Inflammation, impaired development and villus atrophy of the fetal gut was largely prevented by intra-uterine rhIL-1ra treatment. These data form the basis for a clinical understanding of the role of ureaplasma in postnatal intestinal pathologies.</description><identifier>ISSN: 1933-0219</identifier><identifier>EISSN: 1935-3456</identifier><identifier>DOI: 10.1038/mi.2012.97</identifier><identifier>PMID: 23149664</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>631/136 ; 631/250/127/1213 ; 631/250/255/1318 ; 631/250/256 ; Allergology ; Animals ; Antibodies ; Biomedical and Life Sciences ; Biomedicine ; Chorioamnionitis - microbiology ; Disease Models, Animal ; Female ; Gastroenterology ; Humans ; Immunology ; Interleukin 1 Receptor Antagonist Protein - administration & dosage ; Interleukin-1 - immunology ; Intestinal Mucosa - embryology ; Intestinal Mucosa - immunology ; Intestinal Mucosa - microbiology ; Intestines - embryology ; Intestines - immunology ; Intestines - microbiology ; Metagenome - immunology ; Pregnancy ; Prenatal Exposure Delayed Effects - immunology ; Prenatal Exposure Delayed Effects - microbiology ; Sheep, Domestic ; Ureaplasma ; Ureaplasma Infections - complications</subject><ispartof>Mucosal immunology, 2013-05, Vol.6 (3), p.547-556</ispartof><rights>Society for Mucosal Immunology 2013</rights><rights>Copyright Nature Publishing Group May 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-4c8b020911bf4875d8e6ef91e2c3443818eb6960c8e93ec6240782e8f4e293a63</citedby><cites>FETCH-LOGICAL-c387t-4c8b020911bf4875d8e6ef91e2c3443818eb6960c8e93ec6240782e8f4e293a63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23149664$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wolfs, T G A M</creatorcontrib><creatorcontrib>Kallapur, S G</creatorcontrib><creatorcontrib>Knox, C L</creatorcontrib><creatorcontrib>Thuijls, G</creatorcontrib><creatorcontrib>Nitsos, I</creatorcontrib><creatorcontrib>Polglase, G R</creatorcontrib><creatorcontrib>Collins, J J P</creatorcontrib><creatorcontrib>Kroon, E</creatorcontrib><creatorcontrib>Spierings, J</creatorcontrib><creatorcontrib>Shroyer, N F</creatorcontrib><creatorcontrib>Newnham, J P</creatorcontrib><creatorcontrib>Jobe, A H</creatorcontrib><creatorcontrib>Kramer, B W</creatorcontrib><title>Antenatal ureaplasma infection impairs development of the fetal ovine gut in an IL-1-dependent manner</title><title>Mucosal immunology</title><addtitle>Mucosal Immunol</addtitle><addtitle>Mucosal Immunol</addtitle><description>Ureaplasma infection of the amniotic cavity is associated with adverse postnatal intestinal outcomes. We tested whether interleukin-1 (IL-1) signaling underlies intestinal pathology following ureaplasma exposure in fetal sheep. Pregnant ewes received intra-amniotic injections of ureaplasma or culture media for controls at 3, 7, and 14 d before preterm delivery at 124 d gestation (term 150 d). Intra-amniotic injections of recombinant human interleukin IL-1 receptor antagonist (rhIL-1ra) or saline for controls were given 3 h before and every 2 d after Ureaplasma injection. Ureaplasma exposure caused fetal gut inflammation within 7 d with damaged villus epithelium and gut barrier loss. Proliferation, differentiation, and maturation of enterocytes were significantly reduced after 7 d of ureaplasma exposure, leading to severe villus atrophy at 14 d. Inflammation, impaired development and villus atrophy of the fetal gut was largely prevented by intra-uterine rhIL-1ra treatment. 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We tested whether interleukin-1 (IL-1) signaling underlies intestinal pathology following ureaplasma exposure in fetal sheep. Pregnant ewes received intra-amniotic injections of ureaplasma or culture media for controls at 3, 7, and 14 d before preterm delivery at 124 d gestation (term 150 d). Intra-amniotic injections of recombinant human interleukin IL-1 receptor antagonist (rhIL-1ra) or saline for controls were given 3 h before and every 2 d after Ureaplasma injection. Ureaplasma exposure caused fetal gut inflammation within 7 d with damaged villus epithelium and gut barrier loss. Proliferation, differentiation, and maturation of enterocytes were significantly reduced after 7 d of ureaplasma exposure, leading to severe villus atrophy at 14 d. Inflammation, impaired development and villus atrophy of the fetal gut was largely prevented by intra-uterine rhIL-1ra treatment. These data form the basis for a clinical understanding of the role of ureaplasma in postnatal intestinal pathologies.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>23149664</pmid><doi>10.1038/mi.2012.97</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 631/136 631/250/127/1213 631/250/255/1318 631/250/256 Allergology Animals Antibodies Biomedical and Life Sciences Biomedicine Chorioamnionitis - microbiology Disease Models, Animal Female Gastroenterology Humans Immunology Interleukin 1 Receptor Antagonist Protein - administration & dosage Interleukin-1 - immunology Intestinal Mucosa - embryology Intestinal Mucosa - immunology Intestinal Mucosa - microbiology Intestines - embryology Intestines - immunology Intestines - microbiology Metagenome - immunology Pregnancy Prenatal Exposure Delayed Effects - immunology Prenatal Exposure Delayed Effects - microbiology Sheep, Domestic Ureaplasma Ureaplasma Infections - complications |
| title | Antenatal ureaplasma infection impairs development of the fetal ovine gut in an IL-1-dependent manner |
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