Molecular analysis of Malassezia pachydermatis isolated from canine skin and ear in Korea
We investigated Malassezia species and genotypes colonizing dogs, comparing those recovered from the ear canal with those from other anatomical body sites, as well as from diseased and healthy skin. The Malassezia isolates were obtained from four types of skin samples, i.e., diseased ear, diseased s...
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| Veröffentlicht in: | Medical mycology (Oxford) 2013-05, Vol.51 (4), p.396-404 |
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| creator | Han, Seung-Hee Chung, Tae-Ho Nam, Eui-Hwa Park, Seol-Hee Hwang, Cheol-Yong |
| description | We investigated Malassezia species and genotypes colonizing dogs, comparing those recovered from the ear canal with those from other anatomical body sites, as well as from diseased and healthy skin. The Malassezia isolates were obtained from four types of skin samples, i.e., diseased ear, diseased skin, healthy ear, and healthy skin. Sequences of the 26S ribosomal DNA region, the intergenic spacer 1 (IGS-1) and the internal transcribed spacer 1 (ITS-1) DNA region were analyzed. These confirmed the presence of Malassezia pachydermatis, which could be separated into three main sequence genotype groups (A, B, C). Genotype A was the most common, only two genotype B isolates were recovered from diseased skin lesion and genotype C was more likely to be isolated from ear samples than from other healthy or diseased-skin sites. The present findings provide the basis for further studies of genotypic diversity in M. pachydermatis, as well as their pathogenic potential. |
| doi_str_mv | 10.3109/13693786.2012.740575 |
| format | Article |
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The Malassezia isolates were obtained from four types of skin samples, i.e., diseased ear, diseased skin, healthy ear, and healthy skin. Sequences of the 26S ribosomal DNA region, the intergenic spacer 1 (IGS-1) and the internal transcribed spacer 1 (ITS-1) DNA region were analyzed. These confirmed the presence of Malassezia pachydermatis, which could be separated into three main sequence genotype groups (A, B, C). Genotype A was the most common, only two genotype B isolates were recovered from diseased skin lesion and genotype C was more likely to be isolated from ear samples than from other healthy or diseased-skin sites. The present findings provide the basis for further studies of genotypic diversity in M. pachydermatis, as well as their pathogenic potential.</description><identifier>ISSN: 1369-3786</identifier><identifier>EISSN: 1460-2709</identifier><identifier>DOI: 10.3109/13693786.2012.740575</identifier><identifier>PMID: 23167706</identifier><language>eng</language><publisher>UK: Informa Healthcare</publisher><subject>Animals ; Base Sequence ; Dermatomycoses - microbiology ; Dermatomycoses - veterinary ; DNA, Fungal - chemistry ; DNA, Fungal - genetics ; DNA, Intergenic - chemistry ; DNA, Intergenic - genetics ; DNA, Ribosomal Spacer - chemistry ; DNA, Ribosomal Spacer - genetics ; Dog Diseases - microbiology ; Dogs ; Ear Canal - microbiology ; Female ; Genetic Variation ; Genotype ; Malassezia - classification ; Malassezia - genetics ; Malassezia - isolation & purification ; Male ; Molecular Sequence Data ; Phylogeny ; Republic of Korea - epidemiology ; Sebum ; Sequence Alignment - veterinary ; Sequence Analysis, DNA - veterinary ; Skin - microbiology</subject><ispartof>Medical mycology (Oxford), 2013-05, Vol.51 (4), p.396-404</ispartof><rights>2013 ISHAM 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-90e9f3bdf45715e14b76111d0350317fcc0a93282554f0721e4c77af2aaa4f993</citedby><cites>FETCH-LOGICAL-c402t-90e9f3bdf45715e14b76111d0350317fcc0a93282554f0721e4c77af2aaa4f993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23167706$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Han, Seung-Hee</creatorcontrib><creatorcontrib>Chung, Tae-Ho</creatorcontrib><creatorcontrib>Nam, Eui-Hwa</creatorcontrib><creatorcontrib>Park, Seol-Hee</creatorcontrib><creatorcontrib>Hwang, Cheol-Yong</creatorcontrib><title>Molecular analysis of Malassezia pachydermatis isolated from canine skin and ear in Korea</title><title>Medical mycology (Oxford)</title><addtitle>Med Mycol</addtitle><description>We investigated Malassezia species and genotypes colonizing dogs, comparing those recovered from the ear canal with those from other anatomical body sites, as well as from diseased and healthy skin. The Malassezia isolates were obtained from four types of skin samples, i.e., diseased ear, diseased skin, healthy ear, and healthy skin. Sequences of the 26S ribosomal DNA region, the intergenic spacer 1 (IGS-1) and the internal transcribed spacer 1 (ITS-1) DNA region were analyzed. These confirmed the presence of Malassezia pachydermatis, which could be separated into three main sequence genotype groups (A, B, C). Genotype A was the most common, only two genotype B isolates were recovered from diseased skin lesion and genotype C was more likely to be isolated from ear samples than from other healthy or diseased-skin sites. The present findings provide the basis for further studies of genotypic diversity in M. pachydermatis, as well as their pathogenic potential.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Dermatomycoses - microbiology</subject><subject>Dermatomycoses - veterinary</subject><subject>DNA, Fungal - chemistry</subject><subject>DNA, Fungal - genetics</subject><subject>DNA, Intergenic - chemistry</subject><subject>DNA, Intergenic - genetics</subject><subject>DNA, Ribosomal Spacer - chemistry</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>Dog Diseases - microbiology</subject><subject>Dogs</subject><subject>Ear Canal - microbiology</subject><subject>Female</subject><subject>Genetic Variation</subject><subject>Genotype</subject><subject>Malassezia - classification</subject><subject>Malassezia - genetics</subject><subject>Malassezia - isolation & purification</subject><subject>Male</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>Republic of Korea - epidemiology</subject><subject>Sebum</subject><subject>Sequence Alignment - veterinary</subject><subject>Sequence Analysis, DNA - veterinary</subject><subject>Skin - microbiology</subject><issn>1369-3786</issn><issn>1460-2709</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkT1v2zAQhokiRZ2k_QdFoDGLbH5JNJcCgZEmQW10aYdOxJk6wnIo0SGlwf31pSGnY5CJB_B53wOeI-Qro3PBqF4wUWuhlvWcU8bnStJKVR_IJZM1Lbmi-iLPGSlPzIxcpbSnlCnNxScy44LVStH6kvzZBI929BAL6MEfU5uK4IoNeEgJ_7ZQHMDujg3GDob816bgYcCmcDF0hYW-7bFIz22f402BuSaPP0JE-Ew-OvAJv5zfa_L7-_2v1WO5_vnwtLpbl1ZSPpSaonZi2zhZKVYhk1tVM8YaKioqmHLWUtCCL3lVSUcVZyitUuA4AEintbgmt1PvIYaXEdNgujZZ9B56DGMy7BTmS6GXGZUTamNIKaIzh9h2EI-GUXOSal6lmpNUM0nNsZvzhnHbYfM_9GoxA4sJCOPhvZXfpkTbu5DV7hD8sLMQ0ezDGPMl0tsF_wAOrZKj</recordid><startdate>20130501</startdate><enddate>20130501</enddate><creator>Han, Seung-Hee</creator><creator>Chung, Tae-Ho</creator><creator>Nam, Eui-Hwa</creator><creator>Park, Seol-Hee</creator><creator>Hwang, Cheol-Yong</creator><general>Informa Healthcare</general><general>Informa UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130501</creationdate><title>Molecular analysis of Malassezia pachydermatis isolated from canine skin and ear in Korea</title><author>Han, Seung-Hee ; Chung, Tae-Ho ; Nam, Eui-Hwa ; Park, Seol-Hee ; Hwang, Cheol-Yong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-90e9f3bdf45715e14b76111d0350317fcc0a93282554f0721e4c77af2aaa4f993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Dermatomycoses - microbiology</topic><topic>Dermatomycoses - veterinary</topic><topic>DNA, Fungal - chemistry</topic><topic>DNA, Fungal - genetics</topic><topic>DNA, Intergenic - chemistry</topic><topic>DNA, Intergenic - genetics</topic><topic>DNA, Ribosomal Spacer - chemistry</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>Dog Diseases - microbiology</topic><topic>Dogs</topic><topic>Ear Canal - microbiology</topic><topic>Female</topic><topic>Genetic Variation</topic><topic>Genotype</topic><topic>Malassezia - classification</topic><topic>Malassezia - genetics</topic><topic>Malassezia - isolation & purification</topic><topic>Male</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>Republic of Korea - epidemiology</topic><topic>Sebum</topic><topic>Sequence Alignment - veterinary</topic><topic>Sequence Analysis, DNA - veterinary</topic><topic>Skin - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Han, Seung-Hee</creatorcontrib><creatorcontrib>Chung, Tae-Ho</creatorcontrib><creatorcontrib>Nam, Eui-Hwa</creatorcontrib><creatorcontrib>Park, Seol-Hee</creatorcontrib><creatorcontrib>Hwang, Cheol-Yong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Medical mycology (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Han, Seung-Hee</au><au>Chung, Tae-Ho</au><au>Nam, Eui-Hwa</au><au>Park, Seol-Hee</au><au>Hwang, Cheol-Yong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular analysis of Malassezia pachydermatis isolated from canine skin and ear in Korea</atitle><jtitle>Medical mycology (Oxford)</jtitle><addtitle>Med Mycol</addtitle><date>2013-05-01</date><risdate>2013</risdate><volume>51</volume><issue>4</issue><spage>396</spage><epage>404</epage><pages>396-404</pages><issn>1369-3786</issn><eissn>1460-2709</eissn><abstract>We investigated Malassezia species and genotypes colonizing dogs, comparing those recovered from the ear canal with those from other anatomical body sites, as well as from diseased and healthy skin. The Malassezia isolates were obtained from four types of skin samples, i.e., diseased ear, diseased skin, healthy ear, and healthy skin. Sequences of the 26S ribosomal DNA region, the intergenic spacer 1 (IGS-1) and the internal transcribed spacer 1 (ITS-1) DNA region were analyzed. These confirmed the presence of Malassezia pachydermatis, which could be separated into three main sequence genotype groups (A, B, C). Genotype A was the most common, only two genotype B isolates were recovered from diseased skin lesion and genotype C was more likely to be isolated from ear samples than from other healthy or diseased-skin sites. The present findings provide the basis for further studies of genotypic diversity in M. pachydermatis, as well as their pathogenic potential.</abstract><cop>UK</cop><pub>Informa Healthcare</pub><pmid>23167706</pmid><doi>10.3109/13693786.2012.740575</doi><tpages>9</tpages></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Base Sequence Dermatomycoses - microbiology Dermatomycoses - veterinary DNA, Fungal - chemistry DNA, Fungal - genetics DNA, Intergenic - chemistry DNA, Intergenic - genetics DNA, Ribosomal Spacer - chemistry DNA, Ribosomal Spacer - genetics Dog Diseases - microbiology Dogs Ear Canal - microbiology Female Genetic Variation Genotype Malassezia - classification Malassezia - genetics Malassezia - isolation & purification Male Molecular Sequence Data Phylogeny Republic of Korea - epidemiology Sebum Sequence Alignment - veterinary Sequence Analysis, DNA - veterinary Skin - microbiology |
| title | Molecular analysis of Malassezia pachydermatis isolated from canine skin and ear in Korea |
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