Biostimulatory effect of low-level laser therapy on keratinocytes in vitro
Epithelial cells play an important role in reparative events. Therefore, therapies that can stimulate the proliferation and metabolism of these cells could accelerate the healing process. To evaluate the effects of low-level laser therapy (LLLT), human keratinocytes were irradiated with an InGaAsP d...
Gespeichert in:
| Veröffentlicht in: | Lasers in medical science 2013-02, Vol.28 (2), p.367-374 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 374 |
|---|---|
| container_issue | 2 |
| container_start_page | 367 |
| container_title | Lasers in medical science |
| container_volume | 28 |
| creator | Basso, Fernanda G. Oliveira, Camila F. Kurachi, Cristina Hebling, Josimeri Costa, Carlos A. de Souza |
| description | Epithelial cells play an important role in reparative events. Therefore, therapies that can stimulate the proliferation and metabolism of these cells could accelerate the healing process. To evaluate the effects of low-level laser therapy (LLLT), human keratinocytes were irradiated with an InGaAsP diode laser prototype (LASERTable; 780 ± 3 nm; 40 mW) using 0.5, 1.5, 3, 5, and 7 J/cm
2
energy doses. Irradiations were done every 24 h totaling three applications. Evaluation of cell metabolism (MTT assay) showed that LLLT with all energy doses promoted an increase of cell metabolism, being more effective for 0.5, 1.5, and 3 J/cm
2
. The highest cell counts (Trypan blue assay) were observed with 0.5, 3, and 5 J/cm
2
. No statistically significant difference for total protein (TP) production was observed and cell morphology analysis by scanning electron microscopy revealed that LLLT did not promote morphological alterations on the keratinocytes. Real-time polymerase chain reaction (qPCR) revealed that LLLT also promoted an increase of type I collagen (Col-I) and vascular endothelial growth factor (VEGF) gene expression, especially for 1.5 J/cm
2
, but no change on fibroblast growth factor-2 (FGF-2) expression was observed. LLLT at energy doses ranging from 0.5 to 3 J/cm
2
promoted the most significant biostimulatory effects on cultured keratinocytes. |
| doi_str_mv | 10.1007/s10103-012-1057-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1326140435</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1318694608</sourcerecordid><originalsourceid>FETCH-LOGICAL-c443t-b96e2738e9adf4b8c1f5bf194c406ee227a66138d3d71bafca9a27a265d451f33</originalsourceid><addsrcrecordid>eNqNkM1O3TAQRq2qCG4pD8AGedmNi8d2HGcJqH8IiQ2VurOcZFwCufGt7VDl7eurQJeI1YxmznwaHUJOgX8GzuvzBBy4ZBwEA17VzLwjG1CyYpqrX-_JhgttmGkEHJEPKT1wDrUGeUiOhJCgKs035PpyCCkP23l0OcSFovfYZRo8HcNfNuITjnR0CSPN9xjdbqFhoo-ly8MUuiVjosNEn4Ycw0dy4N2Y8OS5HpOfX7_cXX1nN7ffflxd3LBOKZlZ22gUtTTYuN6r1nTgq9ZDozrFNaIQtdPlS9PLvobW-c41rsyErnpVgZfymHxac3cx_JkxZbsdUofj6CYMc7IghQbFi4c3oGB0ozQ3BYUV7WJIKaK3uzhsXVwscLu3bVfbtti2e9t2f3P2HD-3W-z_X7zoLYBYgVRW02-M9iHMcSp2Xkn9BxKcijk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1318694608</pqid></control><display><type>article</type><title>Biostimulatory effect of low-level laser therapy on keratinocytes in vitro</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Basso, Fernanda G. ; Oliveira, Camila F. ; Kurachi, Cristina ; Hebling, Josimeri ; Costa, Carlos A. de Souza</creator><creatorcontrib>Basso, Fernanda G. ; Oliveira, Camila F. ; Kurachi, Cristina ; Hebling, Josimeri ; Costa, Carlos A. de Souza</creatorcontrib><description>Epithelial cells play an important role in reparative events. Therefore, therapies that can stimulate the proliferation and metabolism of these cells could accelerate the healing process. To evaluate the effects of low-level laser therapy (LLLT), human keratinocytes were irradiated with an InGaAsP diode laser prototype (LASERTable; 780 ± 3 nm; 40 mW) using 0.5, 1.5, 3, 5, and 7 J/cm
2
energy doses. Irradiations were done every 24 h totaling three applications. Evaluation of cell metabolism (MTT assay) showed that LLLT with all energy doses promoted an increase of cell metabolism, being more effective for 0.5, 1.5, and 3 J/cm
2
. The highest cell counts (Trypan blue assay) were observed with 0.5, 3, and 5 J/cm
2
. No statistically significant difference for total protein (TP) production was observed and cell morphology analysis by scanning electron microscopy revealed that LLLT did not promote morphological alterations on the keratinocytes. Real-time polymerase chain reaction (qPCR) revealed that LLLT also promoted an increase of type I collagen (Col-I) and vascular endothelial growth factor (VEGF) gene expression, especially for 1.5 J/cm
2
, but no change on fibroblast growth factor-2 (FGF-2) expression was observed. LLLT at energy doses ranging from 0.5 to 3 J/cm
2
promoted the most significant biostimulatory effects on cultured keratinocytes.</description><identifier>ISSN: 0268-8921</identifier><identifier>EISSN: 1435-604X</identifier><identifier>DOI: 10.1007/s10103-012-1057-8</identifier><identifier>PMID: 22314560</identifier><language>eng</language><publisher>London: Springer-Verlag</publisher><subject>Cell Proliferation - radiation effects ; Cells, Cultured ; Collagen Type I - genetics ; Dentistry ; Dose-Response Relationship, Radiation ; Fibroblast Growth Factor 2 - genetics ; Gene Expression Regulation - radiation effects ; Humans ; Keratinocytes - metabolism ; Keratinocytes - radiation effects ; Lasers ; Lasers, Semiconductor - therapeutic use ; Low-Level Light Therapy - instrumentation ; Medicine ; Medicine & Public Health ; Optical Devices ; Optics ; Original Article ; Photonics ; Proteins - metabolism ; Quantum Optics ; Vascular Endothelial Growth Factor A - genetics</subject><ispartof>Lasers in medical science, 2013-02, Vol.28 (2), p.367-374</ispartof><rights>Springer-Verlag London Ltd 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-b96e2738e9adf4b8c1f5bf194c406ee227a66138d3d71bafca9a27a265d451f33</citedby><cites>FETCH-LOGICAL-c443t-b96e2738e9adf4b8c1f5bf194c406ee227a66138d3d71bafca9a27a265d451f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10103-012-1057-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10103-012-1057-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22314560$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Basso, Fernanda G.</creatorcontrib><creatorcontrib>Oliveira, Camila F.</creatorcontrib><creatorcontrib>Kurachi, Cristina</creatorcontrib><creatorcontrib>Hebling, Josimeri</creatorcontrib><creatorcontrib>Costa, Carlos A. de Souza</creatorcontrib><title>Biostimulatory effect of low-level laser therapy on keratinocytes in vitro</title><title>Lasers in medical science</title><addtitle>Lasers Med Sci</addtitle><addtitle>Lasers Med Sci</addtitle><description>Epithelial cells play an important role in reparative events. Therefore, therapies that can stimulate the proliferation and metabolism of these cells could accelerate the healing process. To evaluate the effects of low-level laser therapy (LLLT), human keratinocytes were irradiated with an InGaAsP diode laser prototype (LASERTable; 780 ± 3 nm; 40 mW) using 0.5, 1.5, 3, 5, and 7 J/cm
2
energy doses. Irradiations were done every 24 h totaling three applications. Evaluation of cell metabolism (MTT assay) showed that LLLT with all energy doses promoted an increase of cell metabolism, being more effective for 0.5, 1.5, and 3 J/cm
2
. The highest cell counts (Trypan blue assay) were observed with 0.5, 3, and 5 J/cm
2
. No statistically significant difference for total protein (TP) production was observed and cell morphology analysis by scanning electron microscopy revealed that LLLT did not promote morphological alterations on the keratinocytes. Real-time polymerase chain reaction (qPCR) revealed that LLLT also promoted an increase of type I collagen (Col-I) and vascular endothelial growth factor (VEGF) gene expression, especially for 1.5 J/cm
2
, but no change on fibroblast growth factor-2 (FGF-2) expression was observed. LLLT at energy doses ranging from 0.5 to 3 J/cm
2
promoted the most significant biostimulatory effects on cultured keratinocytes.</description><subject>Cell Proliferation - radiation effects</subject><subject>Cells, Cultured</subject><subject>Collagen Type I - genetics</subject><subject>Dentistry</subject><subject>Dose-Response Relationship, Radiation</subject><subject>Fibroblast Growth Factor 2 - genetics</subject><subject>Gene Expression Regulation - radiation effects</subject><subject>Humans</subject><subject>Keratinocytes - metabolism</subject><subject>Keratinocytes - radiation effects</subject><subject>Lasers</subject><subject>Lasers, Semiconductor - therapeutic use</subject><subject>Low-Level Light Therapy - instrumentation</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Optical Devices</subject><subject>Optics</subject><subject>Original Article</subject><subject>Photonics</subject><subject>Proteins - metabolism</subject><subject>Quantum Optics</subject><subject>Vascular Endothelial Growth Factor A - genetics</subject><issn>0268-8921</issn><issn>1435-604X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkM1O3TAQRq2qCG4pD8AGedmNi8d2HGcJqH8IiQ2VurOcZFwCufGt7VDl7eurQJeI1YxmznwaHUJOgX8GzuvzBBy4ZBwEA17VzLwjG1CyYpqrX-_JhgttmGkEHJEPKT1wDrUGeUiOhJCgKs035PpyCCkP23l0OcSFovfYZRo8HcNfNuITjnR0CSPN9xjdbqFhoo-ly8MUuiVjosNEn4Ycw0dy4N2Y8OS5HpOfX7_cXX1nN7ffflxd3LBOKZlZ22gUtTTYuN6r1nTgq9ZDozrFNaIQtdPlS9PLvobW-c41rsyErnpVgZfymHxac3cx_JkxZbsdUofj6CYMc7IghQbFi4c3oGB0ozQ3BYUV7WJIKaK3uzhsXVwscLu3bVfbtti2e9t2f3P2HD-3W-z_X7zoLYBYgVRW02-M9iHMcSp2Xkn9BxKcijk</recordid><startdate>20130201</startdate><enddate>20130201</enddate><creator>Basso, Fernanda G.</creator><creator>Oliveira, Camila F.</creator><creator>Kurachi, Cristina</creator><creator>Hebling, Josimeri</creator><creator>Costa, Carlos A. de Souza</creator><general>Springer-Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20130201</creationdate><title>Biostimulatory effect of low-level laser therapy on keratinocytes in vitro</title><author>Basso, Fernanda G. ; Oliveira, Camila F. ; Kurachi, Cristina ; Hebling, Josimeri ; Costa, Carlos A. de Souza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-b96e2738e9adf4b8c1f5bf194c406ee227a66138d3d71bafca9a27a265d451f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Cell Proliferation - radiation effects</topic><topic>Cells, Cultured</topic><topic>Collagen Type I - genetics</topic><topic>Dentistry</topic><topic>Dose-Response Relationship, Radiation</topic><topic>Fibroblast Growth Factor 2 - genetics</topic><topic>Gene Expression Regulation - radiation effects</topic><topic>Humans</topic><topic>Keratinocytes - metabolism</topic><topic>Keratinocytes - radiation effects</topic><topic>Lasers</topic><topic>Lasers, Semiconductor - therapeutic use</topic><topic>Low-Level Light Therapy - instrumentation</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Optical Devices</topic><topic>Optics</topic><topic>Original Article</topic><topic>Photonics</topic><topic>Proteins - metabolism</topic><topic>Quantum Optics</topic><topic>Vascular Endothelial Growth Factor A - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Basso, Fernanda G.</creatorcontrib><creatorcontrib>Oliveira, Camila F.</creatorcontrib><creatorcontrib>Kurachi, Cristina</creatorcontrib><creatorcontrib>Hebling, Josimeri</creatorcontrib><creatorcontrib>Costa, Carlos A. de Souza</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Lasers in medical science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Basso, Fernanda G.</au><au>Oliveira, Camila F.</au><au>Kurachi, Cristina</au><au>Hebling, Josimeri</au><au>Costa, Carlos A. de Souza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biostimulatory effect of low-level laser therapy on keratinocytes in vitro</atitle><jtitle>Lasers in medical science</jtitle><stitle>Lasers Med Sci</stitle><addtitle>Lasers Med Sci</addtitle><date>2013-02-01</date><risdate>2013</risdate><volume>28</volume><issue>2</issue><spage>367</spage><epage>374</epage><pages>367-374</pages><issn>0268-8921</issn><eissn>1435-604X</eissn><abstract>Epithelial cells play an important role in reparative events. Therefore, therapies that can stimulate the proliferation and metabolism of these cells could accelerate the healing process. To evaluate the effects of low-level laser therapy (LLLT), human keratinocytes were irradiated with an InGaAsP diode laser prototype (LASERTable; 780 ± 3 nm; 40 mW) using 0.5, 1.5, 3, 5, and 7 J/cm
2
energy doses. Irradiations were done every 24 h totaling three applications. Evaluation of cell metabolism (MTT assay) showed that LLLT with all energy doses promoted an increase of cell metabolism, being more effective for 0.5, 1.5, and 3 J/cm
2
. The highest cell counts (Trypan blue assay) were observed with 0.5, 3, and 5 J/cm
2
. No statistically significant difference for total protein (TP) production was observed and cell morphology analysis by scanning electron microscopy revealed that LLLT did not promote morphological alterations on the keratinocytes. Real-time polymerase chain reaction (qPCR) revealed that LLLT also promoted an increase of type I collagen (Col-I) and vascular endothelial growth factor (VEGF) gene expression, especially for 1.5 J/cm
2
, but no change on fibroblast growth factor-2 (FGF-2) expression was observed. LLLT at energy doses ranging from 0.5 to 3 J/cm
2
promoted the most significant biostimulatory effects on cultured keratinocytes.</abstract><cop>London</cop><pub>Springer-Verlag</pub><pmid>22314560</pmid><doi>10.1007/s10103-012-1057-8</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0268-8921 |
| ispartof | Lasers in medical science, 2013-02, Vol.28 (2), p.367-374 |
| issn | 0268-8921 1435-604X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1326140435 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Cell Proliferation - radiation effects Cells, Cultured Collagen Type I - genetics Dentistry Dose-Response Relationship, Radiation Fibroblast Growth Factor 2 - genetics Gene Expression Regulation - radiation effects Humans Keratinocytes - metabolism Keratinocytes - radiation effects Lasers Lasers, Semiconductor - therapeutic use Low-Level Light Therapy - instrumentation Medicine Medicine & Public Health Optical Devices Optics Original Article Photonics Proteins - metabolism Quantum Optics Vascular Endothelial Growth Factor A - genetics |
| title | Biostimulatory effect of low-level laser therapy on keratinocytes in vitro |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T22%3A40%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Biostimulatory%20effect%20of%20low-level%20laser%20therapy%20on%20keratinocytes%20in%20vitro&rft.jtitle=Lasers%20in%20medical%20science&rft.au=Basso,%20Fernanda%20G.&rft.date=2013-02-01&rft.volume=28&rft.issue=2&rft.spage=367&rft.epage=374&rft.pages=367-374&rft.issn=0268-8921&rft.eissn=1435-604X&rft_id=info:doi/10.1007/s10103-012-1057-8&rft_dat=%3Cproquest_cross%3E1318694608%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1318694608&rft_id=info:pmid/22314560&rfr_iscdi=true |