Evaluation of Biodegradable Peptide Carriers for Light-Directed Targeting
A promising strategy for increased intracellular delivery of nucleic acids with the benefit for targeting is photochemical internalization (PCI). PCI relies on the use of a photosensitizing compound that photochemically destroys membranes in the endocytic pathway after illumination, resulting in cyt...
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| Veröffentlicht in: | Nucleic acid therapeutics 2013-04, Vol.23 (2), p.131-139 |
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| creator | Jørgensen, Jens Andreas Lindin Longva, Ane Sager Hovig, Eivind Bøe, Sigurd Leinæs |
| description | A promising strategy for increased intracellular delivery of nucleic acids with the benefit for targeting is photochemical internalization (PCI). PCI relies on the use of a photosensitizing compound that photochemically destroys membranes in the endocytic pathway after illumination, resulting in cytosolic transfer of endosomal content. PCI technology combined with biodegradable polyamino acid carriers and nucleic acids delivers effective targeting and improved biosafety. In an
in vitro
model system, we have evaluated various poly-
l
-lysine (PLL), poly-
l
-histidine (PLH), and poly-
l
-arginine (PLA) formulations for light-directed small interference RNA (siRNA) gene silencing and messenger RNA (mRNA) delivery. We find that PLA formulations are suitable as siRNA and mRNA carriers in a strictly light-directed manner. |
| doi_str_mv | 10.1089/nat.2012.0403 |
| format | Article |
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in vitro
model system, we have evaluated various poly-
l
-lysine (PLL), poly-
l
-histidine (PLH), and poly-
l
-arginine (PLA) formulations for light-directed small interference RNA (siRNA) gene silencing and messenger RNA (mRNA) delivery. We find that PLA formulations are suitable as siRNA and mRNA carriers in a strictly light-directed manner.</description><identifier>ISSN: 2159-3337</identifier><identifier>EISSN: 2159-3345</identifier><identifier>DOI: 10.1089/nat.2012.0403</identifier><identifier>PMID: 23405950</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject><![CDATA[Arginine - administration & dosage ; Arginine - chemistry ; Biodegradation ; Cell Line, Tumor ; Cell Survival - drug effects ; Drug delivery systems ; Endosomes - chemistry ; Endosomes - metabolism ; Genetic Therapy ; Humans ; Light ; Lysine - administration & dosage ; Lysine - chemistry ; Melanoma - drug therapy ; Melanoma - genetics ; Molecular Targeted Therapy ; Nucleic acids ; Nucleic Acids - administration & dosage ; Nucleic Acids - chemistry ; Original Research Articles ; Peptides - administration & dosage ; Peptides - chemistry ; Pharmacology ; Photochemicals ; Photosensitizing Agents - administration & dosage ; Photosensitizing Agents - chemistry ; Ribonucleic acid ; RNA ; RNA, Small Interfering - administration & dosage ; RNA, Small Interfering - chemistry ; Transfection]]></subject><ispartof>Nucleic acid therapeutics, 2013-04, Vol.23 (2), p.131-139</ispartof><rights>2013, Mary Ann Liebert, Inc.</rights><rights>(©) Copyright 2013, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-972d0ab9fa27d4d4bd127fbaac5a001a913afa12f349657543382c328e168a543</citedby><cites>FETCH-LOGICAL-c431t-972d0ab9fa27d4d4bd127fbaac5a001a913afa12f349657543382c328e168a543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23405950$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jørgensen, Jens Andreas Lindin</creatorcontrib><creatorcontrib>Longva, Ane Sager</creatorcontrib><creatorcontrib>Hovig, Eivind</creatorcontrib><creatorcontrib>Bøe, Sigurd Leinæs</creatorcontrib><title>Evaluation of Biodegradable Peptide Carriers for Light-Directed Targeting</title><title>Nucleic acid therapeutics</title><addtitle>Nucleic Acid Ther</addtitle><description>A promising strategy for increased intracellular delivery of nucleic acids with the benefit for targeting is photochemical internalization (PCI). PCI relies on the use of a photosensitizing compound that photochemically destroys membranes in the endocytic pathway after illumination, resulting in cytosolic transfer of endosomal content. PCI technology combined with biodegradable polyamino acid carriers and nucleic acids delivers effective targeting and improved biosafety. In an
in vitro
model system, we have evaluated various poly-
l
-lysine (PLL), poly-
l
-histidine (PLH), and poly-
l
-arginine (PLA) formulations for light-directed small interference RNA (siRNA) gene silencing and messenger RNA (mRNA) delivery. 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administration & dosage</subject><subject>Photosensitizing Agents - chemistry</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Small Interfering - administration & dosage</subject><subject>RNA, Small Interfering - chemistry</subject><subject>Transfection</subject><issn>2159-3337</issn><issn>2159-3345</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkLtLxEAQhxdRVNTSVgI2NjlnX5dsqef5gAMttA6T7ORcySXn7kbwv3ePUwsbp5kHHz-Gj7FTDhMOpbnsMU4EcDEBBXKHHQquTS6l0ru_sywO2EkIb5BKA6hS77MDIRVoo-GQPcw_sBsxuqHPhja7doOlpUeLdUfZE62js5TN0HtHPmTt4LOFW77G_MZ5aiLZ7Bn9kqLrl8dsr8Uu0Ml3P2Ivt_Pn2X2-eLx7mF0t8kZJHnNTCAtYmxZFYZVVteWiaGvERiMAR8MltshFK5WZ6kIrKUvRSFESn5aY1iN2sc1d--F9pBCrlQsNdR32NIyh4lIoacCUPKHnf9C3YfR9-i5R06nUBjgkKt9SjR9C8NRWa-9W6D8rDtVGc5U0VxvN1UZz4s--U8d6RfaX_pGaALkFNmfs-85RTT7-E_sFkI2HXQ</recordid><startdate>20130401</startdate><enddate>20130401</enddate><creator>Jørgensen, Jens Andreas Lindin</creator><creator>Longva, Ane Sager</creator><creator>Hovig, Eivind</creator><creator>Bøe, Sigurd Leinæs</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20130401</creationdate><title>Evaluation of Biodegradable Peptide Carriers for Light-Directed Targeting</title><author>Jørgensen, Jens Andreas Lindin ; Longva, Ane Sager ; Hovig, Eivind ; Bøe, Sigurd Leinæs</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-972d0ab9fa27d4d4bd127fbaac5a001a913afa12f349657543382c328e168a543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Arginine - administration & dosage</topic><topic>Arginine - chemistry</topic><topic>Biodegradation</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival - drug effects</topic><topic>Drug delivery systems</topic><topic>Endosomes - chemistry</topic><topic>Endosomes - metabolism</topic><topic>Genetic Therapy</topic><topic>Humans</topic><topic>Light</topic><topic>Lysine - administration & dosage</topic><topic>Lysine - chemistry</topic><topic>Melanoma - drug therapy</topic><topic>Melanoma - genetics</topic><topic>Molecular Targeted Therapy</topic><topic>Nucleic acids</topic><topic>Nucleic Acids - administration & dosage</topic><topic>Nucleic Acids - chemistry</topic><topic>Original Research Articles</topic><topic>Peptides - administration & dosage</topic><topic>Peptides - chemistry</topic><topic>Pharmacology</topic><topic>Photochemicals</topic><topic>Photosensitizing Agents - administration & dosage</topic><topic>Photosensitizing Agents - chemistry</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA, Small Interfering - 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Academic</collection><jtitle>Nucleic acid therapeutics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jørgensen, Jens Andreas Lindin</au><au>Longva, Ane Sager</au><au>Hovig, Eivind</au><au>Bøe, Sigurd Leinæs</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of Biodegradable Peptide Carriers for Light-Directed Targeting</atitle><jtitle>Nucleic acid therapeutics</jtitle><addtitle>Nucleic Acid Ther</addtitle><date>2013-04-01</date><risdate>2013</risdate><volume>23</volume><issue>2</issue><spage>131</spage><epage>139</epage><pages>131-139</pages><issn>2159-3337</issn><eissn>2159-3345</eissn><abstract>A promising strategy for increased intracellular delivery of nucleic acids with the benefit for targeting is photochemical internalization (PCI). PCI relies on the use of a photosensitizing compound that photochemically destroys membranes in the endocytic pathway after illumination, resulting in cytosolic transfer of endosomal content. PCI technology combined with biodegradable polyamino acid carriers and nucleic acids delivers effective targeting and improved biosafety. In an
in vitro
model system, we have evaluated various poly-
l
-lysine (PLL), poly-
l
-histidine (PLH), and poly-
l
-arginine (PLA) formulations for light-directed small interference RNA (siRNA) gene silencing and messenger RNA (mRNA) delivery. We find that PLA formulations are suitable as siRNA and mRNA carriers in a strictly light-directed manner.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>23405950</pmid><doi>10.1089/nat.2012.0403</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Nucleic acid therapeutics, 2013-04, Vol.23 (2), p.131-139 |
| issn | 2159-3337 2159-3345 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Arginine - administration & dosage Arginine - chemistry Biodegradation Cell Line, Tumor Cell Survival - drug effects Drug delivery systems Endosomes - chemistry Endosomes - metabolism Genetic Therapy Humans Light Lysine - administration & dosage Lysine - chemistry Melanoma - drug therapy Melanoma - genetics Molecular Targeted Therapy Nucleic acids Nucleic Acids - administration & dosage Nucleic Acids - chemistry Original Research Articles Peptides - administration & dosage Peptides - chemistry Pharmacology Photochemicals Photosensitizing Agents - administration & dosage Photosensitizing Agents - chemistry Ribonucleic acid RNA RNA, Small Interfering - administration & dosage RNA, Small Interfering - chemistry Transfection |
| title | Evaluation of Biodegradable Peptide Carriers for Light-Directed Targeting |
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