A novel method using an acedan-based Zn(DPA) probe to monitor ATP localization in an in vivo system
In in vitro and in vivo systems, understanding localization and the functional role of ATP is essential, but effective methods to monitor ATP in cells and tissues are limited. Although quinacrine dihydrochloride is a well-known fluorescent dye used to detect ATP, it is limited in its use because it...
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| Veröffentlicht in: | Journal of molecular histology 2013-04, Vol.44 (2), p.241-247 |
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| container_title | Journal of molecular histology |
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| creator | Lee, Seo Jin Rao, Alla Sreenivasa Shin, Youn Ho Chung, Hyung-Joo Huh, Youngbuhm Ahn, Kyo Han Jung, Junyang |
| description | In in vitro and in vivo systems, understanding localization and the functional role of ATP is essential, but effective methods to monitor ATP in cells and tissues are limited. Although quinacrine dihydrochloride is a well-known fluorescent dye used to detect ATP, it is limited in its use because it shows non-specific nuclear staining both in vitro and in vivo. A commercial luciferin-luciferase bioluminescence assay has also been used to detect ATP, but it can not be easily used in vivo. Thus, to effectively monitor ATP in vivo, we employed a novel two-photon ATP fluorescent probe, acedan-based Zn(DPA). Using the acedan-based Zn(DPA) probe, we show that this probe produces high quality images of ATP in lung, spleen, liver and spinal cord tissues. |
| doi_str_mv | 10.1007/s10735-012-9474-3 |
| format | Article |
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| subjects | Adenosine Triphosphate - metabolism Animals Biomedical and Life Sciences Biomedicine Brief Communication Cell Biology Developmental Biology Fluorescent Dyes - chemistry Fluorescent Dyes - metabolism Hepatocytes - metabolism Life Sciences Luminescent Measurements - methods Mice Microscopy, Fluorescence - methods Quinacrine - chemistry Spinal Cord - metabolism Spleen - metabolism Zinc - chemistry |
| title | A novel method using an acedan-based Zn(DPA) probe to monitor ATP localization in an in vivo system |
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