A novel method using an acedan-based Zn(DPA) probe to monitor ATP localization in an in vivo system

In in vitro and in vivo systems, understanding localization and the functional role of ATP is essential, but effective methods to monitor ATP in cells and tissues are limited. Although quinacrine dihydrochloride is a well-known fluorescent dye used to detect ATP, it is limited in its use because it...

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Veröffentlicht in:Journal of molecular histology 2013-04, Vol.44 (2), p.241-247
Hauptverfasser: Lee, Seo Jin, Rao, Alla Sreenivasa, Shin, Youn Ho, Chung, Hyung-Joo, Huh, Youngbuhm, Ahn, Kyo Han, Jung, Junyang
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container_end_page 247
container_issue 2
container_start_page 241
container_title Journal of molecular histology
container_volume 44
creator Lee, Seo Jin
Rao, Alla Sreenivasa
Shin, Youn Ho
Chung, Hyung-Joo
Huh, Youngbuhm
Ahn, Kyo Han
Jung, Junyang
description In in vitro and in vivo systems, understanding localization and the functional role of ATP is essential, but effective methods to monitor ATP in cells and tissues are limited. Although quinacrine dihydrochloride is a well-known fluorescent dye used to detect ATP, it is limited in its use because it shows non-specific nuclear staining both in vitro and in vivo. A commercial luciferin-luciferase bioluminescence assay has also been used to detect ATP, but it can not be easily used in vivo. Thus, to effectively monitor ATP in vivo, we employed a novel two-photon ATP fluorescent probe, acedan-based Zn(DPA). Using the acedan-based Zn(DPA) probe, we show that this probe produces high quality images of ATP in lung, spleen, liver and spinal cord tissues.
doi_str_mv 10.1007/s10735-012-9474-3
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subjects Adenosine Triphosphate - metabolism
Animals
Biomedical and Life Sciences
Biomedicine
Brief Communication
Cell Biology
Developmental Biology
Fluorescent Dyes - chemistry
Fluorescent Dyes - metabolism
Hepatocytes - metabolism
Life Sciences
Luminescent Measurements - methods
Mice
Microscopy, Fluorescence - methods
Quinacrine - chemistry
Spinal Cord - metabolism
Spleen - metabolism
Zinc - chemistry
title A novel method using an acedan-based Zn(DPA) probe to monitor ATP localization in an in vivo system
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