Development of transgenic imazapyr-tolerant cowpea (Vigna unguiculata)
KEY MESSAGE : Here we present the development of cowpea lines tolerant to a herbicide from imidazoline class (imazapyr). Plants presented tolerance to fourfold the commercial recommended dose for weed control. Cowpea is one of the most important and widely cultivated legumes in many parts of the wor...
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| Veröffentlicht in: | Plant cell reports 2013-04, Vol.32 (4), p.537-543 |
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| description | KEY MESSAGE : Here we present the development of cowpea lines tolerant to a herbicide from imidazoline class (imazapyr). Plants presented tolerance to fourfold the commercial recommended dose for weed control. Cowpea is one of the most important and widely cultivated legumes in many parts of the world. Its cultivation is drastically affected by weeds, causing damages during growth and development of plants, competing for light, nutrients and water. Consequently, weed control is critical, especially using no-tillage farming systems. In tropical regions, no-till farming is much easier with the use of herbicides to control weeds. This study was conducted to evaluate the possibility of obtaining transgenic cowpea plants resistant to imidazolinone, which would facilitate weed control during the summer season. The biolistic process was used to insert a mutated acetohydroxyacid synthase coding gene (Atahas) which confers tolerance to imazapyr. The transgene integration was confirmed by Southern blot analysis. Out of ten lines tested for tolerance to 100 g ha⁻¹ imazapyr, eight presented some tolerance. One line (named 59) revealed high herbicide tolerance and developmental growth comparable to non-transgenic plants. This line was further tested for tolerance to higher herbicide concentrations and presented tolerance to 400 g ha⁻¹ imazapyr (fourfold the commercial recommended dose) with no visible symptoms. Line 59 will be the foundation for generating imidazolinone-tolerant cowpea varieties, which will facilitate cultivation of this crop in large areas. |
| doi_str_mv | 10.1007/s00299-013-1385-6 |
| format | Article |
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The biolistic process was used to insert a mutated acetohydroxyacid synthase coding gene (Atahas) which confers tolerance to imazapyr. The transgene integration was confirmed by Southern blot analysis. Out of ten lines tested for tolerance to 100 g ha⁻¹ imazapyr, eight presented some tolerance. One line (named 59) revealed high herbicide tolerance and developmental growth comparable to non-transgenic plants. This line was further tested for tolerance to higher herbicide concentrations and presented tolerance to 400 g ha⁻¹ imazapyr (fourfold the commercial recommended dose) with no visible symptoms. 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T</creatorcontrib><creatorcontrib>Cruz, A. R. R</creatorcontrib><creatorcontrib>Aragão, F. J. L</creatorcontrib><title>Development of transgenic imazapyr-tolerant cowpea (Vigna unguiculata)</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><addtitle>Plant Cell Rep</addtitle><description>KEY MESSAGE : Here we present the development of cowpea lines tolerant to a herbicide from imidazoline class (imazapyr). Plants presented tolerance to fourfold the commercial recommended dose for weed control. Cowpea is one of the most important and widely cultivated legumes in many parts of the world. Its cultivation is drastically affected by weeds, causing damages during growth and development of plants, competing for light, nutrients and water. Consequently, weed control is critical, especially using no-tillage farming systems. In tropical regions, no-till farming is much easier with the use of herbicides to control weeds. This study was conducted to evaluate the possibility of obtaining transgenic cowpea plants resistant to imidazolinone, which would facilitate weed control during the summer season. The biolistic process was used to insert a mutated acetohydroxyacid synthase coding gene (Atahas) which confers tolerance to imazapyr. The transgene integration was confirmed by Southern blot analysis. Out of ten lines tested for tolerance to 100 g ha⁻¹ imazapyr, eight presented some tolerance. One line (named 59) revealed high herbicide tolerance and developmental growth comparable to non-transgenic plants. This line was further tested for tolerance to higher herbicide concentrations and presented tolerance to 400 g ha⁻¹ imazapyr (fourfold the commercial recommended dose) with no visible symptoms. Line 59 will be the foundation for generating imidazolinone-tolerant cowpea varieties, which will facilitate cultivation of this crop in large areas.</description><subject>acetolactate synthase</subject><subject>Acetolactate Synthase - genetics</subject><subject>Arabidopsis - enzymology</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>cowpeas</subject><subject>Cultivation</subject><subject>Fabaceae - drug effects</subject><subject>Fabaceae - genetics</subject><subject>Farming systems</subject><subject>genes</subject><subject>Grain cultivation</subject><subject>growth and development</subject><subject>Herbicide Resistance</subject><subject>Herbicides</subject><subject>Herbicides - pharmacology</subject><subject>imazapyr</subject><subject>Imidazoles - pharmacology</subject><subject>Life Sciences</subject><subject>Niacin - analogs & derivatives</subject><subject>Niacin - pharmacology</subject><subject>nutrients</subject><subject>Original Paper</subject><subject>Plant Biochemistry</subject><subject>Plant Sciences</subject><subject>Plants, Genetically Modified - drug effects</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Southern blotting</subject><subject>summer</subject><subject>Transformation, Genetic</subject><subject>Transgenic plants</subject><subject>Tropical environments</subject><subject>tropics</subject><subject>Vigna unguiculata</subject><subject>Weed control</subject><subject>Weeds</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc1u1TAQhS0EopfCA7CBSGzKwjBjJ_5ZokIBqRILKGJnObnjKFVuHOwEVJ4eX6UgxKIrS3O-c2yfYewpwisE0K8zgLCWA0qO0jRc3WM7rKXgAuS3-2wHWiDXGusT9ijna4AiavWQnQgpQSkpd-ziLf2gMc4HmpYqhmpJfso9TUNXDQf_y883iS9xpDJeqi7-nMlXZ1-HfvLVOvXr0K2jX_zLx-xB8GOmJ7fnKbu6ePfl_AO__PT-4_mbS97VVi3c1K1oMBiyoVGmlY3xe-sFmKZukVDtBVGNbUDdaVSEwUMdQqvBiq6IIE_Z2ZY7p_h9pby4w5A7Gkc_UVyzQymkEQBNU9AX_6HXcU1TeV2hUCOIxh4p3KguxZwTBTen8vF04xDcsWS3lexKye5YslPF8-w2eW0PtP_r-NNqAcQG5CJNPaV_rr4j9flmCj4636chu6vPoqwMAKQBZe8k0BoJ8jcJi5hJ</recordid><startdate>20130401</startdate><enddate>20130401</enddate><creator>Citadin, C. 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T ; Cruz, A. R. R ; Aragão, F. J. 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T</au><au>Cruz, A. R. R</au><au>Aragão, F. J. L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of transgenic imazapyr-tolerant cowpea (Vigna unguiculata)</atitle><jtitle>Plant cell reports</jtitle><stitle>Plant Cell Rep</stitle><addtitle>Plant Cell Rep</addtitle><date>2013-04-01</date><risdate>2013</risdate><volume>32</volume><issue>4</issue><spage>537</spage><epage>543</epage><pages>537-543</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><abstract>KEY MESSAGE : Here we present the development of cowpea lines tolerant to a herbicide from imidazoline class (imazapyr). Plants presented tolerance to fourfold the commercial recommended dose for weed control. Cowpea is one of the most important and widely cultivated legumes in many parts of the world. Its cultivation is drastically affected by weeds, causing damages during growth and development of plants, competing for light, nutrients and water. Consequently, weed control is critical, especially using no-tillage farming systems. In tropical regions, no-till farming is much easier with the use of herbicides to control weeds. This study was conducted to evaluate the possibility of obtaining transgenic cowpea plants resistant to imidazolinone, which would facilitate weed control during the summer season. The biolistic process was used to insert a mutated acetohydroxyacid synthase coding gene (Atahas) which confers tolerance to imazapyr. The transgene integration was confirmed by Southern blot analysis. Out of ten lines tested for tolerance to 100 g ha⁻¹ imazapyr, eight presented some tolerance. One line (named 59) revealed high herbicide tolerance and developmental growth comparable to non-transgenic plants. This line was further tested for tolerance to higher herbicide concentrations and presented tolerance to 400 g ha⁻¹ imazapyr (fourfold the commercial recommended dose) with no visible symptoms. Line 59 will be the foundation for generating imidazolinone-tolerant cowpea varieties, which will facilitate cultivation of this crop in large areas.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>23306633</pmid><doi>10.1007/s00299-013-1385-6</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | acetolactate synthase Acetolactate Synthase - genetics Arabidopsis - enzymology Biomedical and Life Sciences Biotechnology Cell Biology cowpeas Cultivation Fabaceae - drug effects Fabaceae - genetics Farming systems genes Grain cultivation growth and development Herbicide Resistance Herbicides Herbicides - pharmacology imazapyr Imidazoles - pharmacology Life Sciences Niacin - analogs & derivatives Niacin - pharmacology nutrients Original Paper Plant Biochemistry Plant Sciences Plants, Genetically Modified - drug effects Plants, Genetically Modified - genetics Southern blotting summer Transformation, Genetic Transgenic plants Tropical environments tropics Vigna unguiculata Weed control Weeds |
| title | Development of transgenic imazapyr-tolerant cowpea (Vigna unguiculata) |
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