Studies on the isolation of Photobacterium damselae subsp. piscicida from diseased golden pompano (Trachinotus ovatus Linnaeus) and antibacterial agents sensitivity
An epizootic occurred among cultured golden pompano (Trachinotus ovatus); it involved mass mortality on fish farms in Linshui in Hainan province, China, in 2008. Diseased fish exhibited no obvious clinical signs, but pathological studies showed that nodules were scattered over the spleen and kidney....
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| Veröffentlicht in: | Veterinary microbiology 2013-03, Vol.162 (2-4), p.957-963 |
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| creator | Wang, Ruixuan Feng, Juan Su, Youlu Ye, Lingtong Wang, Jiangyong |
| description | An epizootic occurred among cultured golden pompano (Trachinotus ovatus); it involved mass mortality on fish farms in Linshui in Hainan province, China, in 2008. Diseased fish exhibited no obvious clinical signs, but pathological studies showed that nodules were scattered over the spleen and kidney. To investigate the nature of the pathogen, we studied the surviving fish, and a Gram-negative bacterium (designated strain TOS1) was isolated from the spleens of golden pompano. Pathogenicity assays revealed that TOS1 was virulent for golden pompano when they were challenged by intraperitoneal injection, and the lethal dose (LD50) was 1.1×106colony forming units (CFU)g−1. 16S rDNA gene sequence of TOS1 demonstrated high similarity (99%) to that of Photobacterium damselae subsp. piscicida. Phylogenetic analysis also showed a clear association of strain TOS1 with P. damselae subsp. piscicida, and this agreed with the results of morphological, physiological and biochemical identification. The results also showed that TOS1 was sensitive to norfloxacin, gentamicin, sulfamethoxazole/trimethoprim, neomycin, streptomycin and tetracycline, and was very sensitive to Psidium guajava and Atractylodes lancea (minimum bactericidal concentration, MBC=10−6g/mL). This paper describes a systematic study of P. damselae subsp. piscicida isolated from diseased golden pompano in China, including its sensitivity to different antibiotics and Chinese herbal extracts, which will contribute to the diagnosis and prevention of the associated disease. |
| doi_str_mv | 10.1016/j.vetmic.2012.09.020 |
| format | Article |
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Diseased fish exhibited no obvious clinical signs, but pathological studies showed that nodules were scattered over the spleen and kidney. To investigate the nature of the pathogen, we studied the surviving fish, and a Gram-negative bacterium (designated strain TOS1) was isolated from the spleens of golden pompano. Pathogenicity assays revealed that TOS1 was virulent for golden pompano when they were challenged by intraperitoneal injection, and the lethal dose (LD50) was 1.1×106colony forming units (CFU)g−1. 16S rDNA gene sequence of TOS1 demonstrated high similarity (99%) to that of Photobacterium damselae subsp. piscicida. Phylogenetic analysis also showed a clear association of strain TOS1 with P. damselae subsp. piscicida, and this agreed with the results of morphological, physiological and biochemical identification. The results also showed that TOS1 was sensitive to norfloxacin, gentamicin, sulfamethoxazole/trimethoprim, neomycin, streptomycin and tetracycline, and was very sensitive to Psidium guajava and Atractylodes lancea (minimum bactericidal concentration, MBC=10−6g/mL). This paper describes a systematic study of P. damselae subsp. piscicida isolated from diseased golden pompano in China, including its sensitivity to different antibiotics and Chinese herbal extracts, which will contribute to the diagnosis and prevention of the associated disease.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2012.09.020</identifier><identifier>PMID: 23140939</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Anti-Bacterial Agents - pharmacology ; Antibacterial agents ; Aquaculture ; Atractylodes lancea ; China ; disease prevention ; DNA, Ribosomal - genetics ; fish ; Fish Diseases - microbiology ; fish farms ; gentamicin ; Gram-negative bacteria ; Gram-Negative Bacterial Infections - microbiology ; Gram-Negative Bacterial Infections - veterinary ; Identification ; intraperitoneal injection ; kidneys ; lethal dose ; Microbial Sensitivity Tests - veterinary ; minimum inhibitory concentration ; mortality ; neomycin ; norfloxacin ; nucleotide sequences ; pathogenicity ; pathogens ; Perciformes ; Photobacterium ; Photobacterium - drug effects ; Photobacterium - genetics ; Photobacterium - isolation & purification ; Photobacterium damselae ; Photobacterium damselae subsp. piscicida ; Phylogeny ; Pompano ; Psidium guajava ; ribosomal DNA ; spleen ; streptomycin ; sulfamethoxazole ; tetracycline ; Trachinotus ; Trachinotus ovatus ; trimethoprim</subject><ispartof>Veterinary microbiology, 2013-03, Vol.162 (2-4), p.957-963</ispartof><rights>2012 Elsevier B.V.</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-321ecfd89ababb9a7c10bbe8ee337964cb33c785ffc57fd42c2019f07a4f5783</citedby><cites>FETCH-LOGICAL-c419t-321ecfd89ababb9a7c10bbe8ee337964cb33c785ffc57fd42c2019f07a4f5783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetmic.2012.09.020$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23140939$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Ruixuan</creatorcontrib><creatorcontrib>Feng, Juan</creatorcontrib><creatorcontrib>Su, Youlu</creatorcontrib><creatorcontrib>Ye, Lingtong</creatorcontrib><creatorcontrib>Wang, Jiangyong</creatorcontrib><title>Studies on the isolation of Photobacterium damselae subsp. piscicida from diseased golden pompano (Trachinotus ovatus Linnaeus) and antibacterial agents sensitivity</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>An epizootic occurred among cultured golden pompano (Trachinotus ovatus); it involved mass mortality on fish farms in Linshui in Hainan province, China, in 2008. Diseased fish exhibited no obvious clinical signs, but pathological studies showed that nodules were scattered over the spleen and kidney. To investigate the nature of the pathogen, we studied the surviving fish, and a Gram-negative bacterium (designated strain TOS1) was isolated from the spleens of golden pompano. Pathogenicity assays revealed that TOS1 was virulent for golden pompano when they were challenged by intraperitoneal injection, and the lethal dose (LD50) was 1.1×106colony forming units (CFU)g−1. 16S rDNA gene sequence of TOS1 demonstrated high similarity (99%) to that of Photobacterium damselae subsp. piscicida. Phylogenetic analysis also showed a clear association of strain TOS1 with P. damselae subsp. piscicida, and this agreed with the results of morphological, physiological and biochemical identification. The results also showed that TOS1 was sensitive to norfloxacin, gentamicin, sulfamethoxazole/trimethoprim, neomycin, streptomycin and tetracycline, and was very sensitive to Psidium guajava and Atractylodes lancea (minimum bactericidal concentration, MBC=10−6g/mL). This paper describes a systematic study of P. damselae subsp. piscicida isolated from diseased golden pompano in China, including its sensitivity to different antibiotics and Chinese herbal extracts, which will contribute to the diagnosis and prevention of the associated disease.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibacterial agents</subject><subject>Aquaculture</subject><subject>Atractylodes lancea</subject><subject>China</subject><subject>disease prevention</subject><subject>DNA, Ribosomal - genetics</subject><subject>fish</subject><subject>Fish Diseases - microbiology</subject><subject>fish farms</subject><subject>gentamicin</subject><subject>Gram-negative bacteria</subject><subject>Gram-Negative Bacterial Infections - microbiology</subject><subject>Gram-Negative Bacterial Infections - veterinary</subject><subject>Identification</subject><subject>intraperitoneal injection</subject><subject>kidneys</subject><subject>lethal dose</subject><subject>Microbial Sensitivity Tests - veterinary</subject><subject>minimum inhibitory concentration</subject><subject>mortality</subject><subject>neomycin</subject><subject>norfloxacin</subject><subject>nucleotide sequences</subject><subject>pathogenicity</subject><subject>pathogens</subject><subject>Perciformes</subject><subject>Photobacterium</subject><subject>Photobacterium - drug effects</subject><subject>Photobacterium - genetics</subject><subject>Photobacterium - isolation & purification</subject><subject>Photobacterium damselae</subject><subject>Photobacterium damselae subsp. piscicida</subject><subject>Phylogeny</subject><subject>Pompano</subject><subject>Psidium guajava</subject><subject>ribosomal DNA</subject><subject>spleen</subject><subject>streptomycin</subject><subject>sulfamethoxazole</subject><subject>tetracycline</subject><subject>Trachinotus</subject><subject>Trachinotus ovatus</subject><subject>trimethoprim</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd2O0zAQhSMEYpeFN0Dgy-WiwT9JE98grVb8SZVA2nJtTexx6yqxg-1U2vfhQXHVwiVcWCPL38zxnFNVrxmtGWXr94f6iHlyuuaU8ZrKmnL6pLpmfSdWvG340-qaiq5fMSbaq-pFSgdKaSPX9Hl1xQVrqBTyuvr1kBfjMJHgSd4jcSmMkF25BUu-70MOA-iM0S0TMTAlHAFJWoY012R2STvtDBAbQ3l2CSGhIbswGvRkDtMMPpDbbQS9dz7kpcgc4VQ2znvAJb0j4E052V1kYCSwQ58TSeiTy-7o8uPL6pmFMeGrS72ptp8-bu-_rDbfPn-9v9usdMNkXgnOUFvTSxhgGCR0mtFhwB5RiE6uGz0Iobu-tVa3nTUN18U6aWkHjW27XtxUt-excww_F0xZTWVDHEfwGJakmOCiZ8U2-X-Ud6KjTRla0OaM6hhSimjVHN0E8VExqk5JqoM6J6lOSSoqVUmytL25KCzDhOZv05_oCvD2DFgICnbRJfXjoUxoKGV92_LTPh_OBBbPjg6jKnmh12hcRJ2VCe7ff_gNtXK_Aw</recordid><startdate>20130323</startdate><enddate>20130323</enddate><creator>Wang, Ruixuan</creator><creator>Feng, Juan</creator><creator>Su, Youlu</creator><creator>Ye, Lingtong</creator><creator>Wang, Jiangyong</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20130323</creationdate><title>Studies on the isolation of Photobacterium damselae subsp. piscicida from diseased golden pompano (Trachinotus ovatus Linnaeus) and antibacterial agents sensitivity</title><author>Wang, Ruixuan ; Feng, Juan ; Su, Youlu ; Ye, Lingtong ; Wang, Jiangyong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-321ecfd89ababb9a7c10bbe8ee337964cb33c785ffc57fd42c2019f07a4f5783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibacterial agents</topic><topic>Aquaculture</topic><topic>Atractylodes lancea</topic><topic>China</topic><topic>disease prevention</topic><topic>DNA, Ribosomal - genetics</topic><topic>fish</topic><topic>Fish Diseases - microbiology</topic><topic>fish farms</topic><topic>gentamicin</topic><topic>Gram-negative bacteria</topic><topic>Gram-Negative Bacterial Infections - microbiology</topic><topic>Gram-Negative Bacterial Infections - veterinary</topic><topic>Identification</topic><topic>intraperitoneal injection</topic><topic>kidneys</topic><topic>lethal dose</topic><topic>Microbial Sensitivity Tests - veterinary</topic><topic>minimum inhibitory concentration</topic><topic>mortality</topic><topic>neomycin</topic><topic>norfloxacin</topic><topic>nucleotide sequences</topic><topic>pathogenicity</topic><topic>pathogens</topic><topic>Perciformes</topic><topic>Photobacterium</topic><topic>Photobacterium - drug effects</topic><topic>Photobacterium - genetics</topic><topic>Photobacterium - isolation & purification</topic><topic>Photobacterium damselae</topic><topic>Photobacterium damselae subsp. piscicida</topic><topic>Phylogeny</topic><topic>Pompano</topic><topic>Psidium guajava</topic><topic>ribosomal DNA</topic><topic>spleen</topic><topic>streptomycin</topic><topic>sulfamethoxazole</topic><topic>tetracycline</topic><topic>Trachinotus</topic><topic>Trachinotus ovatus</topic><topic>trimethoprim</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Ruixuan</creatorcontrib><creatorcontrib>Feng, Juan</creatorcontrib><creatorcontrib>Su, Youlu</creatorcontrib><creatorcontrib>Ye, Lingtong</creatorcontrib><creatorcontrib>Wang, Jiangyong</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Ruixuan</au><au>Feng, Juan</au><au>Su, Youlu</au><au>Ye, Lingtong</au><au>Wang, Jiangyong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Studies on the isolation of Photobacterium damselae subsp. piscicida from diseased golden pompano (Trachinotus ovatus Linnaeus) and antibacterial agents sensitivity</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2013-03-23</date><risdate>2013</risdate><volume>162</volume><issue>2-4</issue><spage>957</spage><epage>963</epage><pages>957-963</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><abstract>An epizootic occurred among cultured golden pompano (Trachinotus ovatus); it involved mass mortality on fish farms in Linshui in Hainan province, China, in 2008. Diseased fish exhibited no obvious clinical signs, but pathological studies showed that nodules were scattered over the spleen and kidney. To investigate the nature of the pathogen, we studied the surviving fish, and a Gram-negative bacterium (designated strain TOS1) was isolated from the spleens of golden pompano. Pathogenicity assays revealed that TOS1 was virulent for golden pompano when they were challenged by intraperitoneal injection, and the lethal dose (LD50) was 1.1×106colony forming units (CFU)g−1. 16S rDNA gene sequence of TOS1 demonstrated high similarity (99%) to that of Photobacterium damselae subsp. piscicida. Phylogenetic analysis also showed a clear association of strain TOS1 with P. damselae subsp. piscicida, and this agreed with the results of morphological, physiological and biochemical identification. The results also showed that TOS1 was sensitive to norfloxacin, gentamicin, sulfamethoxazole/trimethoprim, neomycin, streptomycin and tetracycline, and was very sensitive to Psidium guajava and Atractylodes lancea (minimum bactericidal concentration, MBC=10−6g/mL). This paper describes a systematic study of P. damselae subsp. piscicida isolated from diseased golden pompano in China, including its sensitivity to different antibiotics and Chinese herbal extracts, which will contribute to the diagnosis and prevention of the associated disease.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>23140939</pmid><doi>10.1016/j.vetmic.2012.09.020</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Anti-Bacterial Agents - pharmacology Antibacterial agents Aquaculture Atractylodes lancea China disease prevention DNA, Ribosomal - genetics fish Fish Diseases - microbiology fish farms gentamicin Gram-negative bacteria Gram-Negative Bacterial Infections - microbiology Gram-Negative Bacterial Infections - veterinary Identification intraperitoneal injection kidneys lethal dose Microbial Sensitivity Tests - veterinary minimum inhibitory concentration mortality neomycin norfloxacin nucleotide sequences pathogenicity pathogens Perciformes Photobacterium Photobacterium - drug effects Photobacterium - genetics Photobacterium - isolation & purification Photobacterium damselae Photobacterium damselae subsp. piscicida Phylogeny Pompano Psidium guajava ribosomal DNA spleen streptomycin sulfamethoxazole tetracycline Trachinotus Trachinotus ovatus trimethoprim |
| title | Studies on the isolation of Photobacterium damselae subsp. piscicida from diseased golden pompano (Trachinotus ovatus Linnaeus) and antibacterial agents sensitivity |
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