Novel quantitative TaqMan real‐time PCR assays for detection of Cryptosporidium at the genus level and genotyping of major human and cattle‐infecting species

Aims Development of TaqMan MGB real‐time PCR assays for quantitative typing of major cattle and human‐pathogenic Cryptosporidium species. Methods and Results Three specific TaqMan MGB real‐time PCRs, based on the SSU rRNA gene, were directed towards livestock‐restricted Cryptosporidium andersoni and...

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Veröffentlicht in:	Journal of applied microbiology 2013-04, Vol.114 (4), p.1211-1222
Hauptverfasser:	Burnet, J.B., Ogorzaly, L., Tissier, A., Penny, C., Cauchie, H.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Biological and medical sciences Cattle Cryptosporidium Cryptosporidium - classification Cryptosporidium - genetics Cryptosporidium - isolation & purification Cryptosporidium andersoni Cryptosporidium parvum DNA, Protozoan - genetics Feces - parasitology Fundamental and applied biological sciences. Psychology Genotype Genotype & phenotype genotyping Humans Limit of Detection Microbiology Molecular Sequence Data Oocysts - parasitology Parasite Load Pathology Polymerase chain reaction Real-Time Polymerase Chain Reaction - methods real‐time PCR Ruminantia Sensitivity and Specificity SSU rRNA TaqMan MGB Water - parasitology
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