Current density and conductivity dependent electroporation of Escherichia coli C600
Transformation and surviving of E. coli C600 have been evaluated in dependence on the electric field strength and current density by changing the conductivity of the bacteria suspension. In this context the impact of making bacteria electrocompetent and the addition of NaCl solution was examined. Tr...
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| Veröffentlicht in: | Progress in biophysics and molecular biology 2013-01, Vol.111 (1), p.46-54 |
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| container_title | Progress in biophysics and molecular biology |
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| creator | Brambach, B.C. Michels, A. Franzke, J. Kettler, R. |
| description | Transformation and surviving of E. coli C600 have been evaluated in dependence on the electric field strength and current density by changing the conductivity of the bacteria suspension. In this context the impact of making bacteria electrocompetent and the addition of NaCl solution was examined.
Transformation efficiency declines with increasing conductivity of the suspension. When washing bacteria differently, the transformation efficiency correlates with the number of survivors. In contrary, adding different concentrations of NaCl has no effect on the surviving of E. coli C600.
In dependence on the electric field strength, the transformation efficiency shows no effect on changing the conductivity. Regarding the transformation efficiency in dependence of the current density, a clear shift of the transformation maximum was observed. For higher conductivities, higher current densities are needed to reach the transformation maximum. |
| doi_str_mv | 10.1016/j.pbiomolbio.2012.12.006 |
| format | Article |
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Transformation efficiency declines with increasing conductivity of the suspension. When washing bacteria differently, the transformation efficiency correlates with the number of survivors. In contrary, adding different concentrations of NaCl has no effect on the surviving of E. coli C600.
In dependence on the electric field strength, the transformation efficiency shows no effect on changing the conductivity. Regarding the transformation efficiency in dependence of the current density, a clear shift of the transformation maximum was observed. For higher conductivities, higher current densities are needed to reach the transformation maximum.</description><identifier>ISSN: 0079-6107</identifier><identifier>EISSN: 1873-1732</identifier><identifier>DOI: 10.1016/j.pbiomolbio.2012.12.006</identifier><identifier>PMID: 23274736</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Conductivity ; Current density ; DNA - administration & dosage ; DNA - genetics ; Dose-Response Relationship, Radiation ; Electric Conductivity ; Electroporation ; Electroporation - methods ; Escherichia coli ; Escherichia coli - genetics ; Escherichia coli - radiation effects ; Escherichia coli C600 ; Radiation Dosage ; Transfection - methods</subject><ispartof>Progress in biophysics and molecular biology, 2013-01, Vol.111 (1), p.46-54</ispartof><rights>2012 Elsevier Ltd</rights><rights>Copyright © 2012 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-e40067316de267694b78d65368d447c5800c07f2167ab69fdd072b329a9a08193</citedby><cites>FETCH-LOGICAL-c407t-e40067316de267694b78d65368d447c5800c07f2167ab69fdd072b329a9a08193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.pbiomolbio.2012.12.006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23274736$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brambach, B.C.</creatorcontrib><creatorcontrib>Michels, A.</creatorcontrib><creatorcontrib>Franzke, J.</creatorcontrib><creatorcontrib>Kettler, R.</creatorcontrib><title>Current density and conductivity dependent electroporation of Escherichia coli C600</title><title>Progress in biophysics and molecular biology</title><addtitle>Prog Biophys Mol Biol</addtitle><description>Transformation and surviving of E. coli C600 have been evaluated in dependence on the electric field strength and current density by changing the conductivity of the bacteria suspension. In this context the impact of making bacteria electrocompetent and the addition of NaCl solution was examined.
Transformation efficiency declines with increasing conductivity of the suspension. When washing bacteria differently, the transformation efficiency correlates with the number of survivors. In contrary, adding different concentrations of NaCl has no effect on the surviving of E. coli C600.
In dependence on the electric field strength, the transformation efficiency shows no effect on changing the conductivity. Regarding the transformation efficiency in dependence of the current density, a clear shift of the transformation maximum was observed. For higher conductivities, higher current densities are needed to reach the transformation maximum.</description><subject>Conductivity</subject><subject>Current density</subject><subject>DNA - administration & dosage</subject><subject>DNA - genetics</subject><subject>Dose-Response Relationship, Radiation</subject><subject>Electric Conductivity</subject><subject>Electroporation</subject><subject>Electroporation - methods</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - radiation effects</subject><subject>Escherichia coli C600</subject><subject>Radiation Dosage</subject><subject>Transfection - methods</subject><issn>0079-6107</issn><issn>1873-1732</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1LxDAQhoMouq7-BenRS9dJ0ibtURe_QPCgnkOaTNks3aYmreC_N8uuelQYJhCeN5N5CMkoLChQcbVeDI3zG9-lvmBA2SIVgDggM1pJnlPJ2SGZAcg6FxTkCTmNcQ0AjEpxTE4YZ7KQXMzIy3IKAfsxs9hHN35mureZ8b2dzOg-thcWB-ztFsEOzRj84IMene8z32a30awwOLNyOqU6ly0FwBk5anUX8Xx_zsnb3e3r8iF_er5_XF4_5aYAOeZYpB9LToVFJqSoi0ZWVpRcVLYopCkrAAOyZVRI3Yi6tRYkazirda2hojWfk8vdu0Pw7xPGUW1cNNh1ukc_RUU54xUIDvQfKC0Fk7wsE1rtUBN8jAFbNQS30eFTUVBb-2qtfu2rrX2VKu2Sohf7KVOzQfsT_NadgJsdgEnLh8OgonHYG7QuJLfKevf3lC9LvZm7</recordid><startdate>201301</startdate><enddate>201301</enddate><creator>Brambach, B.C.</creator><creator>Michels, A.</creator><creator>Franzke, J.</creator><creator>Kettler, R.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>201301</creationdate><title>Current density and conductivity dependent electroporation of Escherichia coli C600</title><author>Brambach, B.C. ; Michels, A. ; Franzke, J. ; Kettler, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-e40067316de267694b78d65368d447c5800c07f2167ab69fdd072b329a9a08193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Conductivity</topic><topic>Current density</topic><topic>DNA - administration & dosage</topic><topic>DNA - genetics</topic><topic>Dose-Response Relationship, Radiation</topic><topic>Electric Conductivity</topic><topic>Electroporation</topic><topic>Electroporation - methods</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - radiation effects</topic><topic>Escherichia coli C600</topic><topic>Radiation Dosage</topic><topic>Transfection - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brambach, B.C.</creatorcontrib><creatorcontrib>Michels, A.</creatorcontrib><creatorcontrib>Franzke, J.</creatorcontrib><creatorcontrib>Kettler, R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Progress in biophysics and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brambach, B.C.</au><au>Michels, A.</au><au>Franzke, J.</au><au>Kettler, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Current density and conductivity dependent electroporation of Escherichia coli C600</atitle><jtitle>Progress in biophysics and molecular biology</jtitle><addtitle>Prog Biophys Mol Biol</addtitle><date>2013-01</date><risdate>2013</risdate><volume>111</volume><issue>1</issue><spage>46</spage><epage>54</epage><pages>46-54</pages><issn>0079-6107</issn><eissn>1873-1732</eissn><abstract>Transformation and surviving of E. coli C600 have been evaluated in dependence on the electric field strength and current density by changing the conductivity of the bacteria suspension. In this context the impact of making bacteria electrocompetent and the addition of NaCl solution was examined.
Transformation efficiency declines with increasing conductivity of the suspension. When washing bacteria differently, the transformation efficiency correlates with the number of survivors. In contrary, adding different concentrations of NaCl has no effect on the surviving of E. coli C600.
In dependence on the electric field strength, the transformation efficiency shows no effect on changing the conductivity. Regarding the transformation efficiency in dependence of the current density, a clear shift of the transformation maximum was observed. For higher conductivities, higher current densities are needed to reach the transformation maximum.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>23274736</pmid><doi>10.1016/j.pbiomolbio.2012.12.006</doi><tpages>9</tpages></addata></record> |
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| ispartof | Progress in biophysics and molecular biology, 2013-01, Vol.111 (1), p.46-54 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Conductivity Current density DNA - administration & dosage DNA - genetics Dose-Response Relationship, Radiation Electric Conductivity Electroporation Electroporation - methods Escherichia coli Escherichia coli - genetics Escherichia coli - radiation effects Escherichia coli C600 Radiation Dosage Transfection - methods |
| title | Current density and conductivity dependent electroporation of Escherichia coli C600 |
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