Homologous recombination causes the spontaneous deletion of AVR-Pia in Magnaporthe oryzae
Abstract AVR-Pia, an avirulence gene in the genome of the rice blast fungus Magnaporthe oryzae, triggers a hypersensitive reaction in rice cultivars harbouring the resistance gene Pia. The copy number of AVR-Pia was revealed to vary from one to three among M. oryzae isolates avirulent to Pia rice, a...
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| Veröffentlicht in: | FEMS microbiology letters 2013-02, Vol.339 (2), p.102-109 |
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| creator | Sone, Teruo Takeuchi, Saori Miki, Shinsuke Satoh, Yuki Ohtsuka, Keisuke Abe, Ayumi Asano, Kozo |
| description | Abstract
AVR-Pia, an avirulence gene in the genome of the rice blast fungus Magnaporthe oryzae, triggers a hypersensitive reaction in rice cultivars harbouring the resistance gene Pia. The copy number of AVR-Pia was revealed to vary from one to three among M. oryzae isolates avirulent to Pia rice, and three copies of the gene were located on a single chromosome in strain Ina168, from which the gene was originally cloned. The spontaneous avr-Pia mutant originated from Ina168, named Ina168m95-1, which lacks the AVR-Pia gene, and was therefore used to elucidate the molecular mechanism of the deletion of all three copies of AVR-Pia. Screening and analysis of cosmid clones indicated that two copies of the DNA-type transposon Occan (Occan9E12 and Occan3A3) were located on the same chromosome, and three copies of AVR-Pia were located in between the two Occan elements. Ina168m95-1 contains a conserved Occan element, named Occanm95-1, between sequences homologous to the 5′-flanking region of Occan3A3 and the 3′-flanking region of Occan9E12. In addition, sequence polymorphisms indicated a homologous recombination between Occan3A3 and Occan9E12, which resulted in Occanm95-1. Based on these observations, we propose the hypothesis that homologous recombination in the two Occan elements leads to the deletion of AVR-Pia in Ina168m95-1. |
| doi_str_mv | 10.1111/1574-6968.12058 |
| format | Article |
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AVR-Pia, an avirulence gene in the genome of the rice blast fungus Magnaporthe oryzae, triggers a hypersensitive reaction in rice cultivars harbouring the resistance gene Pia. The copy number of AVR-Pia was revealed to vary from one to three among M. oryzae isolates avirulent to Pia rice, and three copies of the gene were located on a single chromosome in strain Ina168, from which the gene was originally cloned. The spontaneous avr-Pia mutant originated from Ina168, named Ina168m95-1, which lacks the AVR-Pia gene, and was therefore used to elucidate the molecular mechanism of the deletion of all three copies of AVR-Pia. Screening and analysis of cosmid clones indicated that two copies of the DNA-type transposon Occan (Occan9E12 and Occan3A3) were located on the same chromosome, and three copies of AVR-Pia were located in between the two Occan elements. Ina168m95-1 contains a conserved Occan element, named Occanm95-1, between sequences homologous to the 5′-flanking region of Occan3A3 and the 3′-flanking region of Occan9E12. In addition, sequence polymorphisms indicated a homologous recombination between Occan3A3 and Occan9E12, which resulted in Occanm95-1. Based on these observations, we propose the hypothesis that homologous recombination in the two Occan elements leads to the deletion of AVR-Pia in Ina168m95-1.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/1574-6968.12058</identifier><identifier>PMID: 23198972</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Biological and medical sciences ; Chromosomes ; Cultivars ; DNA repair ; DNA Transposable Elements ; DNA, Fungal - chemistry ; DNA, Fungal - genetics ; effector ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; Genes, Fungal ; Homologous Recombination ; loop out ; Magnaporthe - genetics ; Magnaporthe - pathogenicity ; Microbiology ; Molecular Sequence Data ; Oryza - microbiology ; Oryza sativa ; Phytopathology. Animal pests. Plant and forest protection ; Polymorphism, Genetic ; Sequence Analysis, DNA ; Sequence Deletion</subject><ispartof>FEMS microbiology letters, 2013-02, Vol.339 (2), p.102-109</ispartof><rights>2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved 2012</rights><rights>2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><rights>2014 INIST-CNRS</rights><rights>2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.</rights><rights>Copyright © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4698-722628cba78fb46311ee86b2e6b1860a029c3374174b24467dfec07a346406633</citedby><cites>FETCH-LOGICAL-c4698-722628cba78fb46311ee86b2e6b1860a029c3374174b24467dfec07a346406633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2F1574-6968.12058$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2F1574-6968.12058$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26845945$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23198972$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sone, Teruo</creatorcontrib><creatorcontrib>Takeuchi, Saori</creatorcontrib><creatorcontrib>Miki, Shinsuke</creatorcontrib><creatorcontrib>Satoh, Yuki</creatorcontrib><creatorcontrib>Ohtsuka, Keisuke</creatorcontrib><creatorcontrib>Abe, Ayumi</creatorcontrib><creatorcontrib>Asano, Kozo</creatorcontrib><title>Homologous recombination causes the spontaneous deletion of AVR-Pia in Magnaporthe oryzae</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
AVR-Pia, an avirulence gene in the genome of the rice blast fungus Magnaporthe oryzae, triggers a hypersensitive reaction in rice cultivars harbouring the resistance gene Pia. The copy number of AVR-Pia was revealed to vary from one to three among M. oryzae isolates avirulent to Pia rice, and three copies of the gene were located on a single chromosome in strain Ina168, from which the gene was originally cloned. The spontaneous avr-Pia mutant originated from Ina168, named Ina168m95-1, which lacks the AVR-Pia gene, and was therefore used to elucidate the molecular mechanism of the deletion of all three copies of AVR-Pia. Screening and analysis of cosmid clones indicated that two copies of the DNA-type transposon Occan (Occan9E12 and Occan3A3) were located on the same chromosome, and three copies of AVR-Pia were located in between the two Occan elements. Ina168m95-1 contains a conserved Occan element, named Occanm95-1, between sequences homologous to the 5′-flanking region of Occan3A3 and the 3′-flanking region of Occan9E12. In addition, sequence polymorphisms indicated a homologous recombination between Occan3A3 and Occan9E12, which resulted in Occanm95-1. Based on these observations, we propose the hypothesis that homologous recombination in the two Occan elements leads to the deletion of AVR-Pia in Ina168m95-1.</description><subject>Biological and medical sciences</subject><subject>Chromosomes</subject><subject>Cultivars</subject><subject>DNA repair</subject><subject>DNA Transposable Elements</subject><subject>DNA, Fungal - chemistry</subject><subject>DNA, Fungal - genetics</subject><subject>effector</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>Genes, Fungal</subject><subject>Homologous Recombination</subject><subject>loop out</subject><subject>Magnaporthe - genetics</subject><subject>Magnaporthe - pathogenicity</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Oryza - microbiology</subject><subject>Oryza sativa</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Polymorphism, Genetic</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Deletion</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd9L3TAUx8NQ5lX3vDcpyEAG1ZMfTdJHEZ3ClQ3RwZ5Cmnuqlbbpklvk-tfb2quTIZiXQM7n-z3f8CXkK4VDOpwjmimRylzqQ8og05_I7PVlg8yAK51SyNUW2Y7xHgAEA_mZbDFOc50rNiN_zn3ja3_r-5gEdL4pqtYuK98mzvYRY7K8wyR2vl3aFkdogTU-z32ZHP--Sn9VNqna5NLetrbzYcR9WD1a3CWbpa0jflnfO-Tm7PT65Dyd__xxcXI8T52QuU4VY5JpV1ily0JITimilgVDWVAtwQLLHedKUCUKJoRUixIdKMuFFCAl5zvkYPLtgv_bY1yapooO63oKbChnXANjQD9GmWYgNNUwoPv_ofe-D-3wEUMzPnhlGR93H02UCz7GgKXpQtXYsDIUzFiQGeswYx3muaBBsbf27YsGF6_8SyMD8G0N2OhsXQbbuir-46QWWS6ygcsm7qGqcfXRXnN2OX8J8H3S-b57V5W-SfsEd56w-Q</recordid><startdate>201302</startdate><enddate>201302</enddate><creator>Sone, Teruo</creator><creator>Takeuchi, Saori</creator><creator>Miki, Shinsuke</creator><creator>Satoh, Yuki</creator><creator>Ohtsuka, Keisuke</creator><creator>Abe, Ayumi</creator><creator>Asano, Kozo</creator><general>Blackwell Publishing Ltd</general><general>Wiley-Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201302</creationdate><title>Homologous recombination causes the spontaneous deletion of AVR-Pia in Magnaporthe oryzae</title><author>Sone, Teruo ; Takeuchi, Saori ; Miki, Shinsuke ; Satoh, Yuki ; Ohtsuka, Keisuke ; Abe, Ayumi ; Asano, Kozo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4698-722628cba78fb46311ee86b2e6b1860a029c3374174b24467dfec07a346406633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Biological and medical sciences</topic><topic>Chromosomes</topic><topic>Cultivars</topic><topic>DNA repair</topic><topic>DNA Transposable Elements</topic><topic>DNA, Fungal - chemistry</topic><topic>DNA, Fungal - genetics</topic><topic>effector</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal plant pathogens</topic><topic>Genes, Fungal</topic><topic>Homologous Recombination</topic><topic>loop out</topic><topic>Magnaporthe - genetics</topic><topic>Magnaporthe - pathogenicity</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Oryza - microbiology</topic><topic>Oryza sativa</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Polymorphism, Genetic</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Deletion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sone, Teruo</creatorcontrib><creatorcontrib>Takeuchi, Saori</creatorcontrib><creatorcontrib>Miki, Shinsuke</creatorcontrib><creatorcontrib>Satoh, Yuki</creatorcontrib><creatorcontrib>Ohtsuka, Keisuke</creatorcontrib><creatorcontrib>Abe, Ayumi</creatorcontrib><creatorcontrib>Asano, Kozo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sone, Teruo</au><au>Takeuchi, Saori</au><au>Miki, Shinsuke</au><au>Satoh, Yuki</au><au>Ohtsuka, Keisuke</au><au>Abe, Ayumi</au><au>Asano, Kozo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Homologous recombination causes the spontaneous deletion of AVR-Pia in Magnaporthe oryzae</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2013-02</date><risdate>2013</risdate><volume>339</volume><issue>2</issue><spage>102</spage><epage>109</epage><pages>102-109</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
AVR-Pia, an avirulence gene in the genome of the rice blast fungus Magnaporthe oryzae, triggers a hypersensitive reaction in rice cultivars harbouring the resistance gene Pia. The copy number of AVR-Pia was revealed to vary from one to three among M. oryzae isolates avirulent to Pia rice, and three copies of the gene were located on a single chromosome in strain Ina168, from which the gene was originally cloned. The spontaneous avr-Pia mutant originated from Ina168, named Ina168m95-1, which lacks the AVR-Pia gene, and was therefore used to elucidate the molecular mechanism of the deletion of all three copies of AVR-Pia. Screening and analysis of cosmid clones indicated that two copies of the DNA-type transposon Occan (Occan9E12 and Occan3A3) were located on the same chromosome, and three copies of AVR-Pia were located in between the two Occan elements. Ina168m95-1 contains a conserved Occan element, named Occanm95-1, between sequences homologous to the 5′-flanking region of Occan3A3 and the 3′-flanking region of Occan9E12. In addition, sequence polymorphisms indicated a homologous recombination between Occan3A3 and Occan9E12, which resulted in Occanm95-1. Based on these observations, we propose the hypothesis that homologous recombination in the two Occan elements leads to the deletion of AVR-Pia in Ina168m95-1.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>23198972</pmid><doi>10.1111/1574-6968.12058</doi><tpages>8</tpages></addata></record> |
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| ispartof | FEMS microbiology letters, 2013-02, Vol.339 (2), p.102-109 |
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| language | eng |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Biological and medical sciences Chromosomes Cultivars DNA repair DNA Transposable Elements DNA, Fungal - chemistry DNA, Fungal - genetics effector Fundamental and applied biological sciences. Psychology Fungal plant pathogens Genes, Fungal Homologous Recombination loop out Magnaporthe - genetics Magnaporthe - pathogenicity Microbiology Molecular Sequence Data Oryza - microbiology Oryza sativa Phytopathology. Animal pests. Plant and forest protection Polymorphism, Genetic Sequence Analysis, DNA Sequence Deletion |
| title | Homologous recombination causes the spontaneous deletion of AVR-Pia in Magnaporthe oryzae |
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