Development of Real-Time Polymerase Chain Reaction for Detection of Borrelia burgdorferi sensu lato in China

Universal primers and probes were selected on the basis of the 16S rRNA gene sequence of Borrelia burgdorferi in GenBank ® , and a real-time polymerase chain reaction (PCR) method for detection of B. burgdorferi was established. The results showed that this method could specifically detect the B31 s...

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Veröffentlicht in:	Vector borne and zoonotic diseases (Larchmont, N.Y.) N.Y.), 2012-05, Vol.12 (5), p.341-345
Hauptverfasser:	Fu, Yuguang, Liu, Zhijie, Guan, Guiquan, Niu, Qingli, Li, Youquan, Yang, Jifei, Ren, Qiaoyun, Ma, Miling, Liu, Aihong, Peng, Yulv, Luo, Jianxun, Yin, Hong
Format:	Artikel
Sprache:	eng
Schlagworte:	Anaplasma Animals Babesia Blood Borrelia burgdorferi Borrelia burgdorferi Group - genetics Borrelia burgdorferi Group - isolation & purification Cattle Cattle Diseases - epidemiology Cattle Diseases - microbiology China - epidemiology Chlamydia psittaci Cross-reaction Dermacentor - microbiology DNA probes DNA, Bacterial - genetics Genome, Bacterial Genomes genomics Infection Ixodidae Lyme Disease - epidemiology Lyme Disease - microbiology Lyme Disease - veterinary Mycoplasma mycoides Original Research Pathogens Polymerase chain reaction Primers Real-Time Polymerase Chain Reaction - methods rRNA 16S Sensitivity and Specificity Sheep Sheep Diseases - epidemiology Sheep Diseases - microbiology Theileria Vectors
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