Oxygen Concentration and Cysteamine Supplementation During In vitro Production of Buffalo (Bubalus bubalis) Embryos Affect mRNA Expression of BCL‐2, BCL‐XL, MCL‐1, BAX and BID

This study examined the effects of O₂ concentration (5% vs 20%) during in vitro maturation (IVM), fertilization (IVF) and culture (IVC) or supplementation of IVM and IVC media with cysteamine (50 and 100 μm, respectively; IVM, IVF and IVC carried out in 20% O₂), on blastocyst rate and relative mRNA...

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Veröffentlicht in:	Reproduction in domestic animals 2012-12, Vol.47 (6), p.1027-1036
Hauptverfasser:	Elamaran, G, Singh, KP, Singh, MK, Singla, SK, Chauhan, MS, Manik, RS, Palta, P
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal reproduction Animals Antioxidants - administration & dosage Antioxidants - pharmacology Bax protein Bcl-2 protein Bcl-x protein BID protein Biological and medical sciences blastocyst Blastocyst - drug effects Blastocyst - physiology blastocysts Bubalus bubalis Buffalo buffaloes Buffaloes - embryology Cell culture Culture Media cysteamine Cysteamine - pharmacology Developmental stages Embryo Culture Techniques - veterinary Embryogenesis Embryos Fertilization Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation, Developmental - physiology genes in vitro fertilization Mammalian reproduction. General aspects Mcl-1 protein Media (culture) messenger RNA Oocytes Oxygen Oxygen - pharmacology Proto-Oncogene Proteins c-bcl-2 - genetics Proto-Oncogene Proteins c-bcl-2 - metabolism quantitative polymerase chain reaction Real-Time Polymerase Chain Reaction - veterinary RNA, Messenger - genetics RNA, Messenger - metabolism Supplementation Vertebrates: reproduction
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