Oxygen Concentration and Cysteamine Supplementation During In vitro Production of Buffalo (Bubalus bubalis) Embryos Affect mRNA Expression of BCL‐2, BCL‐XL, MCL‐1, BAX and BID

This study examined the effects of O₂ concentration (5% vs 20%) during in vitro maturation (IVM), fertilization (IVF) and culture (IVC) or supplementation of IVM and IVC media with cysteamine (50 and 100 μm, respectively; IVM, IVF and IVC carried out in 20% O₂), on blastocyst rate and relative mRNA...

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Veröffentlicht in:	Reproduction in domestic animals 2012-12, Vol.47 (6), p.1027-1036
Hauptverfasser:	Elamaran, G, Singh, KP, Singh, MK, Singla, SK, Chauhan, MS, Manik, RS, Palta, P
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal reproduction Animals Antioxidants - administration & dosage Antioxidants - pharmacology Bax protein Bcl-2 protein Bcl-x protein BID protein Biological and medical sciences blastocyst Blastocyst - drug effects Blastocyst - physiology blastocysts Bubalus bubalis Buffalo buffaloes Buffaloes - embryology Cell culture Culture Media cysteamine Cysteamine - pharmacology Developmental stages Embryo Culture Techniques - veterinary Embryogenesis Embryos Fertilization Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation, Developmental - physiology genes in vitro fertilization Mammalian reproduction. General aspects Mcl-1 protein Media (culture) messenger RNA Oocytes Oxygen Oxygen - pharmacology Proto-Oncogene Proteins c-bcl-2 - genetics Proto-Oncogene Proteins c-bcl-2 - metabolism quantitative polymerase chain reaction Real-Time Polymerase Chain Reaction - veterinary RNA, Messenger - genetics RNA, Messenger - metabolism Supplementation Vertebrates: reproduction
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Zusammenfassung:	This study examined the effects of O₂ concentration (5% vs 20%) during in vitro maturation (IVM), fertilization (IVF) and culture (IVC) or supplementation of IVM and IVC media with cysteamine (50 and 100 μm, respectively; IVM, IVF and IVC carried out in 20% O₂), on blastocyst rate and relative mRNA abundance of some apoptosis‐related genes measured by real‐time qPCR in immature and in vitro‐matured buffalo oocytes and in embryos at 2‐, 4‐, 8‐ to 16‐cell, morula and blastocyst stages. The blastocyst rate was significantly higher (p
ISSN:	0936-6768 1439-0531
DOI:	10.1111/j.1439-0531.2012.02009.x