A cotton LIM domain-containing protein (GhWLIM5) is involved in bundling actin filaments
LIM-domain proteins play important roles in cellular processes in eukaryotes. In this study, a LIM protein gene, GhWLIM5, was identified in cotton. Quantitative RT-PCR analysis showed that GhWLIM5 was expressed widely in different cotton tissues and had a peak in expression during fiber elongation....
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| Veröffentlicht in: | Plant physiology and biochemistry 2013-05, Vol.66, p.34-40 |
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| creator | Li, Yang Jiang, Jia Li, Lan Wang, Xiu-Lan Wang, Na-Na Li, Deng-Di Li, Xue-Bao |
| description | LIM-domain proteins play important roles in cellular processes in eukaryotes. In this study, a LIM protein gene, GhWLIM5, was identified in cotton. Quantitative RT-PCR analysis showed that GhWLIM5 was expressed widely in different cotton tissues and had a peak in expression during fiber elongation. GFP fluorescence assay revealed that cotton cells expressing GhWLIM5:eGFP fusion gene displayed a network distribution of eGFP fluorescence, suggesting that GhWLIM5 protein is mainly localized to the cell cytoskeleton. When GhWLIM5:eGFP transformed cells were stained with rhodamine-phalloidin there was consistent overlap in eGFP and rhodamine-palloidin signals, demonstrating that GhWLIM5 protein is colocalized with the F-actin cytoskeleton. In addition, high-speed cosedimentation assay verified that GhWLIM5 directly bound actin filaments, while low cosedimentation assay and microscopic observation indicated that GhWLIM5 bundled F-actin in vitro. Increasing amounts of GhWLIM5 protein were able to protect F-actin from depolymerization in vitro in the presence of Lat B (an F-actin depolymerizer). Our results contribute to a better understanding of the biochemical role of GhWLIM5 in modulating the dynamic F-actin network in cotton.
► GhWLIM5 gene is expressed widely in different cotton tissues. ► Distribution of GhWLIM5 proteins is associated with actin cytoskeleton in cells. ► GhWLIM5 proteins bundle actin filaments for modulating dynamic actin cytoskeleton. ► GhWLIM5 may play an important role in protecting F-actin from depolymerization. |
| doi_str_mv | 10.1016/j.plaphy.2013.01.018 |
| format | Article |
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► GhWLIM5 gene is expressed widely in different cotton tissues. ► Distribution of GhWLIM5 proteins is associated with actin cytoskeleton in cells. ► GhWLIM5 proteins bundle actin filaments for modulating dynamic actin cytoskeleton. ► GhWLIM5 may play an important role in protecting F-actin from depolymerization.</description><identifier>ISSN: 0981-9428</identifier><identifier>EISSN: 1873-2690</identifier><identifier>DOI: 10.1016/j.plaphy.2013.01.018</identifier><identifier>PMID: 23466745</identifier><identifier>CODEN: PPBIEX</identifier><language>eng</language><publisher>Paris: Elsevier Masson SAS</publisher><subject>actin ; Actin Cytoskeleton - metabolism ; Actin filaments ; Actins - metabolism ; Amino Acid Sequence ; Biological and medical sciences ; Bundling ; cotton ; Cotton (Gossypium hirsutum) ; depolymerization ; eukaryotic cells ; fluorescence ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Plant ; gene fusion ; genes ; Genes, Plant ; Gossypium - classification ; Gossypium - genetics ; Gossypium - metabolism ; Green Fluorescent Proteins - metabolism ; LIM Domain Proteins - genetics ; LIM Domain Proteins - metabolism ; LIM domain-containing protein ; microfilaments ; Molecular Sequence Data ; Phalloidine - analogs & derivatives ; Phalloidine - metabolism ; Phylogeny ; Plant physiology and development ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Protein Binding ; Protein Interaction Mapping ; Recombinant Fusion Proteins - metabolism ; reverse transcriptase polymerase chain reaction ; Rhodamines - metabolism</subject><ispartof>Plant physiology and biochemistry, 2013-05, Vol.66, p.34-40</ispartof><rights>2013 Elsevier Masson SAS</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2013 Elsevier Masson SAS. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-46c58cded0cd064f45f95faa4a24e64ace56b65d3fe090263c4fbeeaabd807063</citedby><cites>FETCH-LOGICAL-c416t-46c58cded0cd064f45f95faa4a24e64ace56b65d3fe090263c4fbeeaabd807063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0981942813000387$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27212485$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23466745$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Yang</creatorcontrib><creatorcontrib>Jiang, Jia</creatorcontrib><creatorcontrib>Li, Lan</creatorcontrib><creatorcontrib>Wang, Xiu-Lan</creatorcontrib><creatorcontrib>Wang, Na-Na</creatorcontrib><creatorcontrib>Li, Deng-Di</creatorcontrib><creatorcontrib>Li, Xue-Bao</creatorcontrib><title>A cotton LIM domain-containing protein (GhWLIM5) is involved in bundling actin filaments</title><title>Plant physiology and biochemistry</title><addtitle>Plant Physiol Biochem</addtitle><description>LIM-domain proteins play important roles in cellular processes in eukaryotes. In this study, a LIM protein gene, GhWLIM5, was identified in cotton. Quantitative RT-PCR analysis showed that GhWLIM5 was expressed widely in different cotton tissues and had a peak in expression during fiber elongation. GFP fluorescence assay revealed that cotton cells expressing GhWLIM5:eGFP fusion gene displayed a network distribution of eGFP fluorescence, suggesting that GhWLIM5 protein is mainly localized to the cell cytoskeleton. When GhWLIM5:eGFP transformed cells were stained with rhodamine-phalloidin there was consistent overlap in eGFP and rhodamine-palloidin signals, demonstrating that GhWLIM5 protein is colocalized with the F-actin cytoskeleton. In addition, high-speed cosedimentation assay verified that GhWLIM5 directly bound actin filaments, while low cosedimentation assay and microscopic observation indicated that GhWLIM5 bundled F-actin in vitro. Increasing amounts of GhWLIM5 protein were able to protect F-actin from depolymerization in vitro in the presence of Lat B (an F-actin depolymerizer). Our results contribute to a better understanding of the biochemical role of GhWLIM5 in modulating the dynamic F-actin network in cotton.
► GhWLIM5 gene is expressed widely in different cotton tissues. ► Distribution of GhWLIM5 proteins is associated with actin cytoskeleton in cells. ► GhWLIM5 proteins bundle actin filaments for modulating dynamic actin cytoskeleton. ► GhWLIM5 may play an important role in protecting F-actin from depolymerization.</description><subject>actin</subject><subject>Actin Cytoskeleton - metabolism</subject><subject>Actin filaments</subject><subject>Actins - metabolism</subject><subject>Amino Acid Sequence</subject><subject>Biological and medical sciences</subject><subject>Bundling</subject><subject>cotton</subject><subject>Cotton (Gossypium hirsutum)</subject><subject>depolymerization</subject><subject>eukaryotic cells</subject><subject>fluorescence</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Plant</subject><subject>gene fusion</subject><subject>genes</subject><subject>Genes, Plant</subject><subject>Gossypium - classification</subject><subject>Gossypium - genetics</subject><subject>Gossypium - metabolism</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>LIM Domain Proteins - genetics</subject><subject>LIM Domain Proteins - metabolism</subject><subject>LIM domain-containing protein</subject><subject>microfilaments</subject><subject>Molecular Sequence Data</subject><subject>Phalloidine - analogs & derivatives</subject><subject>Phalloidine - metabolism</subject><subject>Phylogeny</subject><subject>Plant physiology and development</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Protein Binding</subject><subject>Protein Interaction Mapping</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>Rhodamines - metabolism</subject><issn>0981-9428</issn><issn>1873-2690</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90c9rFDEUB_Agil2r_4HoXIR6mPXlx2RmLkIpWgsrHrToLWSSlzbLTLKdZBf635tlVr0JD5LA5-WFbwh5TWFNgcoP2_Vu1Lv7xzUDytdAS3VPyIp2La-Z7OEpWUHf0boXrDsjL1LaAgATLX9OzhgXUraiWZFfl5WJOcdQbW6-VjZO2ofaxJDL6sNdtZtjRh-qi-v7n0U07yufKh8OcTygLZtq2Ac7HqU2uRydH_WEIaeX5JnTY8JXp_Wc3H7-9OPqS735dn1zdbmpjaAy10KapjMWLRgLUjjRuL5xWgvNBEqhDTZykI3lDqEHJrkRbkDUerAdtCD5OblY7i0vfdhjymryyeA46oBxnxTljLWcSeCFioWaOaY0o1O72U96flQU1DFTtVVLpuqYqQJaqittb04T9sOE9m_TnxALeHcCOhk9ulkH49M_1zLKRHd0bxfndFT6bi7m9nuZ1JSPob3s-yI-LgJLYgePs0rGYzBo_YwmKxv9_9_6GxRmn9o</recordid><startdate>20130501</startdate><enddate>20130501</enddate><creator>Li, Yang</creator><creator>Jiang, Jia</creator><creator>Li, Lan</creator><creator>Wang, Xiu-Lan</creator><creator>Wang, Na-Na</creator><creator>Li, Deng-Di</creator><creator>Li, Xue-Bao</creator><general>Elsevier Masson SAS</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130501</creationdate><title>A cotton LIM domain-containing protein (GhWLIM5) is involved in bundling actin filaments</title><author>Li, Yang ; Jiang, Jia ; Li, Lan ; Wang, Xiu-Lan ; Wang, Na-Na ; Li, Deng-Di ; Li, Xue-Bao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-46c58cded0cd064f45f95faa4a24e64ace56b65d3fe090263c4fbeeaabd807063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>actin</topic><topic>Actin Cytoskeleton - metabolism</topic><topic>Actin filaments</topic><topic>Actins - metabolism</topic><topic>Amino Acid Sequence</topic><topic>Biological and medical sciences</topic><topic>Bundling</topic><topic>cotton</topic><topic>Cotton (Gossypium hirsutum)</topic><topic>depolymerization</topic><topic>eukaryotic cells</topic><topic>fluorescence</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Plant</topic><topic>gene fusion</topic><topic>genes</topic><topic>Genes, Plant</topic><topic>Gossypium - classification</topic><topic>Gossypium - genetics</topic><topic>Gossypium - metabolism</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>LIM Domain Proteins - genetics</topic><topic>LIM Domain Proteins - metabolism</topic><topic>LIM domain-containing protein</topic><topic>microfilaments</topic><topic>Molecular Sequence Data</topic><topic>Phalloidine - analogs & derivatives</topic><topic>Phalloidine - metabolism</topic><topic>Phylogeny</topic><topic>Plant physiology and development</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Protein Binding</topic><topic>Protein Interaction Mapping</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>reverse transcriptase polymerase chain reaction</topic><topic>Rhodamines - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Yang</creatorcontrib><creatorcontrib>Jiang, Jia</creatorcontrib><creatorcontrib>Li, Lan</creatorcontrib><creatorcontrib>Wang, Xiu-Lan</creatorcontrib><creatorcontrib>Wang, Na-Na</creatorcontrib><creatorcontrib>Li, Deng-Di</creatorcontrib><creatorcontrib>Li, Xue-Bao</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Yang</au><au>Jiang, Jia</au><au>Li, Lan</au><au>Wang, Xiu-Lan</au><au>Wang, Na-Na</au><au>Li, Deng-Di</au><au>Li, Xue-Bao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A cotton LIM domain-containing protein (GhWLIM5) is involved in bundling actin filaments</atitle><jtitle>Plant physiology and biochemistry</jtitle><addtitle>Plant Physiol Biochem</addtitle><date>2013-05-01</date><risdate>2013</risdate><volume>66</volume><spage>34</spage><epage>40</epage><pages>34-40</pages><issn>0981-9428</issn><eissn>1873-2690</eissn><coden>PPBIEX</coden><abstract>LIM-domain proteins play important roles in cellular processes in eukaryotes. In this study, a LIM protein gene, GhWLIM5, was identified in cotton. Quantitative RT-PCR analysis showed that GhWLIM5 was expressed widely in different cotton tissues and had a peak in expression during fiber elongation. GFP fluorescence assay revealed that cotton cells expressing GhWLIM5:eGFP fusion gene displayed a network distribution of eGFP fluorescence, suggesting that GhWLIM5 protein is mainly localized to the cell cytoskeleton. When GhWLIM5:eGFP transformed cells were stained with rhodamine-phalloidin there was consistent overlap in eGFP and rhodamine-palloidin signals, demonstrating that GhWLIM5 protein is colocalized with the F-actin cytoskeleton. In addition, high-speed cosedimentation assay verified that GhWLIM5 directly bound actin filaments, while low cosedimentation assay and microscopic observation indicated that GhWLIM5 bundled F-actin in vitro. Increasing amounts of GhWLIM5 protein were able to protect F-actin from depolymerization in vitro in the presence of Lat B (an F-actin depolymerizer). Our results contribute to a better understanding of the biochemical role of GhWLIM5 in modulating the dynamic F-actin network in cotton.
► GhWLIM5 gene is expressed widely in different cotton tissues. ► Distribution of GhWLIM5 proteins is associated with actin cytoskeleton in cells. ► GhWLIM5 proteins bundle actin filaments for modulating dynamic actin cytoskeleton. ► GhWLIM5 may play an important role in protecting F-actin from depolymerization.</abstract><cop>Paris</cop><pub>Elsevier Masson SAS</pub><pmid>23466745</pmid><doi>10.1016/j.plaphy.2013.01.018</doi><tpages>7</tpages></addata></record> |
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| subjects | actin Actin Cytoskeleton - metabolism Actin filaments Actins - metabolism Amino Acid Sequence Biological and medical sciences Bundling cotton Cotton (Gossypium hirsutum) depolymerization eukaryotic cells fluorescence Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant gene fusion genes Genes, Plant Gossypium - classification Gossypium - genetics Gossypium - metabolism Green Fluorescent Proteins - metabolism LIM Domain Proteins - genetics LIM Domain Proteins - metabolism LIM domain-containing protein microfilaments Molecular Sequence Data Phalloidine - analogs & derivatives Phalloidine - metabolism Phylogeny Plant physiology and development Plant Proteins - genetics Plant Proteins - metabolism Protein Binding Protein Interaction Mapping Recombinant Fusion Proteins - metabolism reverse transcriptase polymerase chain reaction Rhodamines - metabolism |
| title | A cotton LIM domain-containing protein (GhWLIM5) is involved in bundling actin filaments |
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