An opioid growth factor receptor (OGFR) for [Met5]-enkephalin in Chlamys farreri
Opioid growth factor receptor (OGFR) is a receptor for [Met5]-enkephalin and plays important roles in the regulation of cell growth and embryonic development. In the present study, a cDNA of 2381 bp for the scallop Chlamys farreri OGFR (designated as CfOGFR) was identified by rapid amplification of...
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| Veröffentlicht in: | Fish & shellfish immunology 2013-05, Vol.34 (5), p.1228-1235 |
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| creator | Guo, Ying Wang, Lingling Zhou, Zhi Wang, Mengqiang Liu, Rui Wang, Leilei Jiang, Qiufen Song, Linsheng |
| description | Opioid growth factor receptor (OGFR) is a receptor for [Met5]-enkephalin and plays important roles in the regulation of cell growth and embryonic development. In the present study, a cDNA of 2381 bp for the scallop Chlamys farreri OGFR (designated as CfOGFR) was identified by rapid amplification of cDNA ends (RACE) approach and expression sequence tag (EST) analysis. The complete cDNA sequence of CfOGFR contained an open reading frame (ORF) of 1200 bp, which encoded a protein of 399 amino acids. The amino acid sequence of CfOGFR shared 33–64% similarity with other OGFRs. There was a low complexity domain and a conserved OGFR_N domain at the N-terminal of CfOGFR. The mRNA transcripts of CfOGFR were constitutively expressed in the tested tissues with the highest expression level in hepatopancreas. During the early embryonic development, the mRNA transcripts of CfOGFR could be detected in different development stages, where the expression level presented a downward trend as a whole. The stimulations of LPS, Glu and poly (I:C) significantly induced the expression of CfOGFR mRNA in hemocytes (P |
| doi_str_mv | 10.1016/j.fsi.2013.02.002 |
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► An invertebrate OGFR (CfOGFR) was identified from scallop. ► CfOGFR could bind [Met5]-enkephalin and inhibited cell proliferation. ► PAMP stimulation could induce the expression of CfOGFR. ► CfOGFR involved in the ontogenesis and immune response of scallop.</description><identifier>ISSN: 1050-4648</identifier><identifier>EISSN: 1095-9947</identifier><identifier>DOI: 10.1016/j.fsi.2013.02.002</identifier><identifier>PMID: 23462147</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>[Met5]-enkephalin ; Amino Acid Sequence ; Animals ; Base Sequence ; beta-Glucans - administration & dosage ; Blotting, Western ; Cell Proliferation ; Chlamys farreri ; Cloning, Molecular ; DNA, Complementary - genetics ; DNA, Complementary - metabolism ; Electrophoresis, Polyacrylamide Gel ; Hemocytes - immunology ; Hemocytes - metabolism ; Immune response ; Immunity, Innate ; Molecular Sequence Data ; Opioid growth factor receptor ; Organ Specificity ; Pectinidae - chemistry ; Pectinidae - genetics ; Pectinidae - immunology ; Pectinidae - metabolism ; Phylogeny ; Poly I-C - administration & dosage ; Polymerase Chain Reaction ; Polysaccharides, Bacterial - administration & dosage ; Receptors, Opioid - chemistry ; Receptors, Opioid - genetics ; Receptors, Opioid - immunology ; Receptors, Opioid - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sequence Alignment</subject><ispartof>Fish & shellfish immunology, 2013-05, Vol.34 (5), p.1228-1235</ispartof><rights>2013 Elsevier Ltd</rights><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-5c80e3203c26157784316c44d0b19db36e82e807b7f8c08e1801ee91c96c6d8c3</citedby><cites>FETCH-LOGICAL-c353t-5c80e3203c26157784316c44d0b19db36e82e807b7f8c08e1801ee91c96c6d8c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fsi.2013.02.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23462147$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, Ying</creatorcontrib><creatorcontrib>Wang, Lingling</creatorcontrib><creatorcontrib>Zhou, Zhi</creatorcontrib><creatorcontrib>Wang, Mengqiang</creatorcontrib><creatorcontrib>Liu, Rui</creatorcontrib><creatorcontrib>Wang, Leilei</creatorcontrib><creatorcontrib>Jiang, Qiufen</creatorcontrib><creatorcontrib>Song, Linsheng</creatorcontrib><title>An opioid growth factor receptor (OGFR) for [Met5]-enkephalin in Chlamys farreri</title><title>Fish & shellfish immunology</title><addtitle>Fish Shellfish Immunol</addtitle><description>Opioid growth factor receptor (OGFR) is a receptor for [Met5]-enkephalin and plays important roles in the regulation of cell growth and embryonic development. In the present study, a cDNA of 2381 bp for the scallop Chlamys farreri OGFR (designated as CfOGFR) was identified by rapid amplification of cDNA ends (RACE) approach and expression sequence tag (EST) analysis. The complete cDNA sequence of CfOGFR contained an open reading frame (ORF) of 1200 bp, which encoded a protein of 399 amino acids. The amino acid sequence of CfOGFR shared 33–64% similarity with other OGFRs. There was a low complexity domain and a conserved OGFR_N domain at the N-terminal of CfOGFR. The mRNA transcripts of CfOGFR were constitutively expressed in the tested tissues with the highest expression level in hepatopancreas. During the early embryonic development, the mRNA transcripts of CfOGFR could be detected in different development stages, where the expression level presented a downward trend as a whole. The stimulations of LPS, Glu and poly (I:C) significantly induced the expression of CfOGFR mRNA in hemocytes (P < 0.05), while PGN stimulation exerted no influence. Co-IP and western blot results revealed that the CfOGFR in hemocytes displayed high affinity and specificity to [Met5]-enkephalin. Exogenous [Met5]-enkephalin was observed to inhibit the proliferation of HEK293T cells transfected with pcDNA3.1(+)-CfOGFR in a time and dosage dependent manner. These results collectively indicated that CfOGFR, as a homolog of OGFRs in C. farreri, played an important role in cells proliferation, and might be involved in the immune response of scallops.
► An invertebrate OGFR (CfOGFR) was identified from scallop. ► CfOGFR could bind [Met5]-enkephalin and inhibited cell proliferation. ► PAMP stimulation could induce the expression of CfOGFR. ► CfOGFR involved in the ontogenesis and immune response of scallop.</description><subject>[Met5]-enkephalin</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>beta-Glucans - administration & dosage</subject><subject>Blotting, Western</subject><subject>Cell Proliferation</subject><subject>Chlamys farreri</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary - genetics</subject><subject>DNA, Complementary - metabolism</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Hemocytes - immunology</subject><subject>Hemocytes - metabolism</subject><subject>Immune response</subject><subject>Immunity, Innate</subject><subject>Molecular Sequence Data</subject><subject>Opioid growth factor receptor</subject><subject>Organ Specificity</subject><subject>Pectinidae - chemistry</subject><subject>Pectinidae - genetics</subject><subject>Pectinidae - immunology</subject><subject>Pectinidae - metabolism</subject><subject>Phylogeny</subject><subject>Poly I-C - administration & dosage</subject><subject>Polymerase Chain Reaction</subject><subject>Polysaccharides, Bacterial - administration & dosage</subject><subject>Receptors, Opioid - chemistry</subject><subject>Receptors, Opioid - genetics</subject><subject>Receptors, Opioid - immunology</subject><subject>Receptors, Opioid - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Alignment</subject><issn>1050-4648</issn><issn>1095-9947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLw0AUhQdRrK8f4Eay1EXinUcmE1xJsVVQKqIrkSGd3NipaRJnUqX_3imtLoWBexbfOTAfIacUEgpUXs6TytuEAeUJsASA7ZADCnka57nIdtc5hVhIoQbk0Ps5AEguYZ8MGBeSUZEdkMfrJmo729oyenftdz-LqsL0rYscGuzW4XwyHj1dRFWIrw_Yp28xNh_YzYraNlF4w1ldLFY-9JxDZ4_JXlXUHk-294i8jG6eh7fx_WR8N7y-jw1PeR-nRgFyBtwwSdMsU4JTaYQoYUrzcsolKoYKsmlWKQMKqQKKmFOTSyNLZfgROd_sdq79XKLv9cJ6g3VdNNguvaacsYwDiDygdIMa13rvsNKds4vCrTQFvRap5zqI1GuRGpgOIkPnbDu_nC6w_Gv8mgvA1QbA8Mkvi057Y7ExWNqgrtdla_-Z_wEpFIE9</recordid><startdate>20130501</startdate><enddate>20130501</enddate><creator>Guo, Ying</creator><creator>Wang, Lingling</creator><creator>Zhou, Zhi</creator><creator>Wang, Mengqiang</creator><creator>Liu, Rui</creator><creator>Wang, Leilei</creator><creator>Jiang, Qiufen</creator><creator>Song, Linsheng</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130501</creationdate><title>An opioid growth factor receptor (OGFR) for [Met5]-enkephalin in Chlamys farreri</title><author>Guo, Ying ; Wang, Lingling ; Zhou, Zhi ; Wang, Mengqiang ; Liu, Rui ; Wang, Leilei ; Jiang, Qiufen ; Song, Linsheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-5c80e3203c26157784316c44d0b19db36e82e807b7f8c08e1801ee91c96c6d8c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>[Met5]-enkephalin</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>beta-Glucans - administration & dosage</topic><topic>Blotting, Western</topic><topic>Cell Proliferation</topic><topic>Chlamys farreri</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary - genetics</topic><topic>DNA, Complementary - metabolism</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Hemocytes - immunology</topic><topic>Hemocytes - metabolism</topic><topic>Immune response</topic><topic>Immunity, Innate</topic><topic>Molecular Sequence Data</topic><topic>Opioid growth factor receptor</topic><topic>Organ Specificity</topic><topic>Pectinidae - chemistry</topic><topic>Pectinidae - genetics</topic><topic>Pectinidae - immunology</topic><topic>Pectinidae - metabolism</topic><topic>Phylogeny</topic><topic>Poly I-C - administration & dosage</topic><topic>Polymerase Chain Reaction</topic><topic>Polysaccharides, Bacterial - administration & dosage</topic><topic>Receptors, Opioid - chemistry</topic><topic>Receptors, Opioid - genetics</topic><topic>Receptors, Opioid - immunology</topic><topic>Receptors, Opioid - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Alignment</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Ying</creatorcontrib><creatorcontrib>Wang, Lingling</creatorcontrib><creatorcontrib>Zhou, Zhi</creatorcontrib><creatorcontrib>Wang, Mengqiang</creatorcontrib><creatorcontrib>Liu, Rui</creatorcontrib><creatorcontrib>Wang, Leilei</creatorcontrib><creatorcontrib>Jiang, Qiufen</creatorcontrib><creatorcontrib>Song, Linsheng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fish & shellfish immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Ying</au><au>Wang, Lingling</au><au>Zhou, Zhi</au><au>Wang, Mengqiang</au><au>Liu, Rui</au><au>Wang, Leilei</au><au>Jiang, Qiufen</au><au>Song, Linsheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An opioid growth factor receptor (OGFR) for [Met5]-enkephalin in Chlamys farreri</atitle><jtitle>Fish & shellfish immunology</jtitle><addtitle>Fish Shellfish Immunol</addtitle><date>2013-05-01</date><risdate>2013</risdate><volume>34</volume><issue>5</issue><spage>1228</spage><epage>1235</epage><pages>1228-1235</pages><issn>1050-4648</issn><eissn>1095-9947</eissn><abstract>Opioid growth factor receptor (OGFR) is a receptor for [Met5]-enkephalin and plays important roles in the regulation of cell growth and embryonic development. In the present study, a cDNA of 2381 bp for the scallop Chlamys farreri OGFR (designated as CfOGFR) was identified by rapid amplification of cDNA ends (RACE) approach and expression sequence tag (EST) analysis. The complete cDNA sequence of CfOGFR contained an open reading frame (ORF) of 1200 bp, which encoded a protein of 399 amino acids. The amino acid sequence of CfOGFR shared 33–64% similarity with other OGFRs. There was a low complexity domain and a conserved OGFR_N domain at the N-terminal of CfOGFR. The mRNA transcripts of CfOGFR were constitutively expressed in the tested tissues with the highest expression level in hepatopancreas. During the early embryonic development, the mRNA transcripts of CfOGFR could be detected in different development stages, where the expression level presented a downward trend as a whole. The stimulations of LPS, Glu and poly (I:C) significantly induced the expression of CfOGFR mRNA in hemocytes (P < 0.05), while PGN stimulation exerted no influence. Co-IP and western blot results revealed that the CfOGFR in hemocytes displayed high affinity and specificity to [Met5]-enkephalin. Exogenous [Met5]-enkephalin was observed to inhibit the proliferation of HEK293T cells transfected with pcDNA3.1(+)-CfOGFR in a time and dosage dependent manner. These results collectively indicated that CfOGFR, as a homolog of OGFRs in C. farreri, played an important role in cells proliferation, and might be involved in the immune response of scallops.
► An invertebrate OGFR (CfOGFR) was identified from scallop. ► CfOGFR could bind [Met5]-enkephalin and inhibited cell proliferation. ► PAMP stimulation could induce the expression of CfOGFR. ► CfOGFR involved in the ontogenesis and immune response of scallop.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>23462147</pmid><doi>10.1016/j.fsi.2013.02.002</doi><tpages>8</tpages></addata></record> |
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| subjects | [Met5]-enkephalin Amino Acid Sequence Animals Base Sequence beta-Glucans - administration & dosage Blotting, Western Cell Proliferation Chlamys farreri Cloning, Molecular DNA, Complementary - genetics DNA, Complementary - metabolism Electrophoresis, Polyacrylamide Gel Hemocytes - immunology Hemocytes - metabolism Immune response Immunity, Innate Molecular Sequence Data Opioid growth factor receptor Organ Specificity Pectinidae - chemistry Pectinidae - genetics Pectinidae - immunology Pectinidae - metabolism Phylogeny Poly I-C - administration & dosage Polymerase Chain Reaction Polysaccharides, Bacterial - administration & dosage Receptors, Opioid - chemistry Receptors, Opioid - genetics Receptors, Opioid - immunology Receptors, Opioid - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Alignment |
| title | An opioid growth factor receptor (OGFR) for [Met5]-enkephalin in Chlamys farreri |
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