Microbial surface display of glucose dehydrogenase for amperometric glucose biosensor

A genetically engineered Escherichia coli (E. coli) strain displaying glucose dehydrogenase (GDH) with ice-nucleation protein (INP) as the anchoring motif was first constructed. The surface localization and functionality of the fusion protein containing GDH were verified by SDS-PAGE, Western blottin...

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Veröffentlicht in:	Biosensors & bioelectronics 2013-07, Vol.45, p.19-24
Hauptverfasser:	Liang, Bo, Li, Liang, Tang, XiangJiang, Lang, Qiaolin, Wang, Hongwei, Li, Feng, Shi, Jianguo, Shen, Wei, Palchetti, Ilaria, Mascini, Marco, Liu, Aihua
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial electrode Bacterial Outer Membrane Proteins - chemistry Bacterial surface display Biological and medical sciences Biosensing Techniques - methods Biosensors Biotechnology Electrochemical glucose biosensor Electrochemistry Electrodes Escherichia coli - enzymology Fundamental and applied biological sciences. Psychology Glucose - isolation & purification Glucose 1-Dehydrogenase - chemistry Glucose dehydrogenase displayed bacteria Humans Limit of Detection Methods. Procedures. Technologies Nanotubes, Carbon - chemistry Oxidation-Reduction Saccharide analysis Various methods and equipments
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