Melatonin protects rat thymus against oxidative stress caused by exposure to microwaves and modulates proliferation/apoptosis of thymocytes
The aim of the study was to evaluate the effect of melatonin on oxidative stress, DNA fragmentation, apoptsis and proliferation in thymus tissue of rats exposed to microwaves. Wistar rats were divided in four groups: I - treated with saline; II - treated with melatonin; III - microwaves exposed; IV...
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| Veröffentlicht in: | General physiology and biophysics 2013-03, Vol.32 (1), p.79-90 |
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| creator | Sokolovic, Dusan Djordjevic, Branka Kocic, Gordana Veljkovic, Andrej Marinkovic, Milena Basic, Jelena Jevtovic-Stoimenov, Tatjana Stanojkovic, Zoran Sokolovic, Danka M Pavlovic, Voja Djindjic, Boris Krstic, Dejan |
| description | The aim of the study was to evaluate the effect of melatonin on oxidative stress, DNA fragmentation, apoptsis and proliferation in thymus tissue of rats exposed to microwaves. Wistar rats were divided in four groups: I - treated with saline; II - treated with melatonin; III - microwaves exposed; IV - microwaves exposed and melatonin treated. Melatonin (2 mg/kg i.p.) was administered daily. Animals were sacrificed after 20, 40 and 60 days. A significant increase in malondialdehyde and carbonyl group content, as well as decrease in catalase and increase in xanthine oxidase activity were registered under microwave exposure. Melatonin prevented the increase in malondialdehyde and carbonyl group content, and reversed the effect on catalase and xanthine oxidase activity. Both, alkaline and acid DNase activity were increased due to microwave exposure. Furthermore, microwaves caused increase in apoptosis rate (detected using Annexin V-FITC/PI kit) and reduced proliferative capacity of thymocytes (induced by ConA). However, melatonin caused decrease in alkaline and acid DNase activity, decrease in apoptotic rate and increase in proliferation rate of thymocytes. Melatonin exerts protective effects on rat thymocytes by modulating processes of apoptosis and proliferation, and causes decrease in DNA fragmentation and oxidative stress intensity under exposure to microwaves. |
| doi_str_mv | 10.4149/gpb_2013002 |
| format | Article |
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Wistar rats were divided in four groups: I - treated with saline; II - treated with melatonin; III - microwaves exposed; IV - microwaves exposed and melatonin treated. Melatonin (2 mg/kg i.p.) was administered daily. Animals were sacrificed after 20, 40 and 60 days. A significant increase in malondialdehyde and carbonyl group content, as well as decrease in catalase and increase in xanthine oxidase activity were registered under microwave exposure. Melatonin prevented the increase in malondialdehyde and carbonyl group content, and reversed the effect on catalase and xanthine oxidase activity. Both, alkaline and acid DNase activity were increased due to microwave exposure. Furthermore, microwaves caused increase in apoptosis rate (detected using Annexin V-FITC/PI kit) and reduced proliferative capacity of thymocytes (induced by ConA). However, melatonin caused decrease in alkaline and acid DNase activity, decrease in apoptotic rate and increase in proliferation rate of thymocytes. 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Wistar rats were divided in four groups: I - treated with saline; II - treated with melatonin; III - microwaves exposed; IV - microwaves exposed and melatonin treated. Melatonin (2 mg/kg i.p.) was administered daily. Animals were sacrificed after 20, 40 and 60 days. A significant increase in malondialdehyde and carbonyl group content, as well as decrease in catalase and increase in xanthine oxidase activity were registered under microwave exposure. Melatonin prevented the increase in malondialdehyde and carbonyl group content, and reversed the effect on catalase and xanthine oxidase activity. Both, alkaline and acid DNase activity were increased due to microwave exposure. Furthermore, microwaves caused increase in apoptosis rate (detected using Annexin V-FITC/PI kit) and reduced proliferative capacity of thymocytes (induced by ConA). However, melatonin caused decrease in alkaline and acid DNase activity, decrease in apoptotic rate and increase in proliferation rate of thymocytes. Melatonin exerts protective effects on rat thymocytes by modulating processes of apoptosis and proliferation, and causes decrease in DNA fragmentation and oxidative stress intensity under exposure to microwaves.</description><subject>Animals</subject><subject>Antioxidants - pharmacology</subject><subject>Apoptosis</subject><subject>Catalase - metabolism</subject><subject>Cell Proliferation</subject><subject>Deoxyribonucleases - metabolism</subject><subject>DNA Fragmentation</subject><subject>Male</subject><subject>Melatonin - pharmacology</subject><subject>Microwaves</subject><subject>Oxidative Stress</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Thymocytes - cytology</subject><subject>Thymus Gland - drug effects</subject><subject>Thymus Gland - metabolism</subject><subject>Thymus Gland - radiation effects</subject><subject>Time Factors</subject><subject>Xanthine Oxidase - metabolism</subject><issn>0231-5882</issn><issn>1338-4325</issn><issn>1338-4325</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkUtLxDAQgIMouqyevEuOglTzaLvZoyy-YMWLnkuaTNZI29ROurq_wT9tdFXMZQh8882LkGPOznOezy9WfV0JxiVjYodMuJQqy6UodsmECcmzQilxQI4QX1h6xWwuBNsnB0IWkis5m5CPe2h0DJ3vaD-ECCYiHXSk8XnTjkj1SvsOIw3v3uro10AxDoBIjR4RLK03FN77gOMANAbaejOEN72GlNlZ2gY7Jnv6JXfjHSSzD92F7kMfA3qkwX1XCmaTqEOy53SDcPQTp-Tp-upxcZstH27uFpfLzEhRxkxZNWMyt8rIUoJxplTGiLrMmZWOFc7N5awuhWIgnLa2BAFc6zS6ZkIXKXVKTrfe1NXrCBir1qOBptEdhBErLkVaZMFEntCzLZrmQhzAVf3gWz1sKs6qrwNU_w6Q6JMf8Vi3YP_Y33XLT3h2hYE</recordid><startdate>20130301</startdate><enddate>20130301</enddate><creator>Sokolovic, Dusan</creator><creator>Djordjevic, Branka</creator><creator>Kocic, Gordana</creator><creator>Veljkovic, Andrej</creator><creator>Marinkovic, Milena</creator><creator>Basic, Jelena</creator><creator>Jevtovic-Stoimenov, Tatjana</creator><creator>Stanojkovic, Zoran</creator><creator>Sokolovic, Danka M</creator><creator>Pavlovic, Voja</creator><creator>Djindjic, Boris</creator><creator>Krstic, Dejan</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130301</creationdate><title>Melatonin protects rat thymus against oxidative stress caused by exposure to microwaves and modulates proliferation/apoptosis of thymocytes</title><author>Sokolovic, Dusan ; Djordjevic, Branka ; Kocic, Gordana ; Veljkovic, Andrej ; Marinkovic, Milena ; Basic, Jelena ; Jevtovic-Stoimenov, Tatjana ; Stanojkovic, Zoran ; Sokolovic, Danka M ; Pavlovic, Voja ; Djindjic, Boris ; Krstic, Dejan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-8d87034d8c363ecfc68cc2b640d3f05ff937b6280e2fadd6e2e1aa792a02a5703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Antioxidants - pharmacology</topic><topic>Apoptosis</topic><topic>Catalase - metabolism</topic><topic>Cell Proliferation</topic><topic>Deoxyribonucleases - metabolism</topic><topic>DNA Fragmentation</topic><topic>Male</topic><topic>Melatonin - pharmacology</topic><topic>Microwaves</topic><topic>Oxidative Stress</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Thymocytes - cytology</topic><topic>Thymus Gland - drug effects</topic><topic>Thymus Gland - metabolism</topic><topic>Thymus Gland - radiation effects</topic><topic>Time Factors</topic><topic>Xanthine Oxidase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sokolovic, Dusan</creatorcontrib><creatorcontrib>Djordjevic, Branka</creatorcontrib><creatorcontrib>Kocic, Gordana</creatorcontrib><creatorcontrib>Veljkovic, Andrej</creatorcontrib><creatorcontrib>Marinkovic, Milena</creatorcontrib><creatorcontrib>Basic, Jelena</creatorcontrib><creatorcontrib>Jevtovic-Stoimenov, Tatjana</creatorcontrib><creatorcontrib>Stanojkovic, Zoran</creatorcontrib><creatorcontrib>Sokolovic, Danka M</creatorcontrib><creatorcontrib>Pavlovic, Voja</creatorcontrib><creatorcontrib>Djindjic, Boris</creatorcontrib><creatorcontrib>Krstic, Dejan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>General physiology and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sokolovic, Dusan</au><au>Djordjevic, Branka</au><au>Kocic, Gordana</au><au>Veljkovic, Andrej</au><au>Marinkovic, Milena</au><au>Basic, Jelena</au><au>Jevtovic-Stoimenov, Tatjana</au><au>Stanojkovic, Zoran</au><au>Sokolovic, Danka M</au><au>Pavlovic, Voja</au><au>Djindjic, Boris</au><au>Krstic, Dejan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Melatonin protects rat thymus against oxidative stress caused by exposure to microwaves and modulates proliferation/apoptosis of thymocytes</atitle><jtitle>General physiology and biophysics</jtitle><addtitle>Gen Physiol Biophys</addtitle><date>2013-03-01</date><risdate>2013</risdate><volume>32</volume><issue>1</issue><spage>79</spage><epage>90</epage><pages>79-90</pages><issn>0231-5882</issn><issn>1338-4325</issn><eissn>1338-4325</eissn><abstract>The aim of the study was to evaluate the effect of melatonin on oxidative stress, DNA fragmentation, apoptsis and proliferation in thymus tissue of rats exposed to microwaves. Wistar rats were divided in four groups: I - treated with saline; II - treated with melatonin; III - microwaves exposed; IV - microwaves exposed and melatonin treated. Melatonin (2 mg/kg i.p.) was administered daily. Animals were sacrificed after 20, 40 and 60 days. A significant increase in malondialdehyde and carbonyl group content, as well as decrease in catalase and increase in xanthine oxidase activity were registered under microwave exposure. Melatonin prevented the increase in malondialdehyde and carbonyl group content, and reversed the effect on catalase and xanthine oxidase activity. Both, alkaline and acid DNase activity were increased due to microwave exposure. Furthermore, microwaves caused increase in apoptosis rate (detected using Annexin V-FITC/PI kit) and reduced proliferative capacity of thymocytes (induced by ConA). However, melatonin caused decrease in alkaline and acid DNase activity, decrease in apoptotic rate and increase in proliferation rate of thymocytes. 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| ispartof | General physiology and biophysics, 2013-03, Vol.32 (1), p.79-90 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Antioxidants - pharmacology Apoptosis Catalase - metabolism Cell Proliferation Deoxyribonucleases - metabolism DNA Fragmentation Male Melatonin - pharmacology Microwaves Oxidative Stress Rats Rats, Wistar Thymocytes - cytology Thymus Gland - drug effects Thymus Gland - metabolism Thymus Gland - radiation effects Time Factors Xanthine Oxidase - metabolism |
| title | Melatonin protects rat thymus against oxidative stress caused by exposure to microwaves and modulates proliferation/apoptosis of thymocytes |
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