Characterization and Quantitation of Anthocyanins in Purple-Fleshed Sweet Potatoes Cultivated in Korea by HPLC-DAD and HPLC-ESI-QTOF-MS/MS
The characterization and quantitative analysis of anthocyanins in four purple-fleshed sweet potato varieties (Borami, Mokpo 62, Shinzami, and Zami) cultivated in Korea were carried out by HPLC/diode array detector (DAD), HPLC-TOF/MS, and HPLC-MS/MS analyses. For the identification of anthocyanins, m...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2013-03, Vol.61 (12), p.3148-3158 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Lee, Mi Jin Park, Jeong Seob Choi, Dong Seong Jung, Mun Yhung |
| description | The characterization and quantitative analysis of anthocyanins in four purple-fleshed sweet potato varieties (Borami, Mokpo 62, Shinzami, and Zami) cultivated in Korea were carried out by HPLC/diode array detector (DAD), HPLC-TOF/MS, and HPLC-MS/MS analyses. For the identification of anthocyanins, molecular formulas were first calculated by using the exact mass data of the molecular ions ([M]+). The patterns of isotope ions of M+ were also monitored to confirm the assignment of the molecular formulas. HPLC-MS2 analysis was further conducted for elucidating their molecular structures. Twenty-seven different anthocyanins were tentatively identified in the sweet potatoes. Six of them are the first reported in sweet potatoes roots. The quantity and profiles of anthocyanins in sweet potatoes varied greatly with variety. Borami was found, for the first time, to be a rare sweet potato variety with an exceptionally high quantity of pelargonidin-based anthocyanins. |
| doi_str_mv | 10.1021/jf3055455 |
| format | Article |
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For the identification of anthocyanins, molecular formulas were first calculated by using the exact mass data of the molecular ions ([M]+). The patterns of isotope ions of M+ were also monitored to confirm the assignment of the molecular formulas. HPLC-MS2 analysis was further conducted for elucidating their molecular structures. Twenty-seven different anthocyanins were tentatively identified in the sweet potatoes. Six of them are the first reported in sweet potatoes roots. The quantity and profiles of anthocyanins in sweet potatoes varied greatly with variety. Borami was found, for the first time, to be a rare sweet potato variety with an exceptionally high quantity of pelargonidin-based anthocyanins.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf3055455</identifier><identifier>PMID: 23464823</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>anthocyanins ; Anthocyanins - analysis ; Anthocyanins - chemistry ; Anthocyanins - isolation & purification ; chemical structure ; Chromatography, High Pressure Liquid ; high performance liquid chromatography ; ions ; Ipomoea batatas - chemistry ; isotopes ; Mass Spectrometry ; Plant Tubers - chemistry ; quantitative analysis ; Republic of Korea ; roots ; Species Specificity ; sweet potatoes</subject><ispartof>Journal of agricultural and food chemistry, 2013-03, Vol.61 (12), p.3148-3158</ispartof><rights>Copyright © 2013 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a339t-394a69d3f47d267f744a9e36c5e527aff2e9335387644a2d2ad47e38da9a462f3</citedby><cites>FETCH-LOGICAL-a339t-394a69d3f47d267f744a9e36c5e527aff2e9335387644a2d2ad47e38da9a462f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf3055455$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf3055455$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,777,781,2753,27058,27906,27907,56720,56770</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23464823$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Mi Jin</creatorcontrib><creatorcontrib>Park, Jeong Seob</creatorcontrib><creatorcontrib>Choi, Dong Seong</creatorcontrib><creatorcontrib>Jung, Mun Yhung</creatorcontrib><title>Characterization and Quantitation of Anthocyanins in Purple-Fleshed Sweet Potatoes Cultivated in Korea by HPLC-DAD and HPLC-ESI-QTOF-MS/MS</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>The characterization and quantitative analysis of anthocyanins in four purple-fleshed sweet potato varieties (Borami, Mokpo 62, Shinzami, and Zami) cultivated in Korea were carried out by HPLC/diode array detector (DAD), HPLC-TOF/MS, and HPLC-MS/MS analyses. For the identification of anthocyanins, molecular formulas were first calculated by using the exact mass data of the molecular ions ([M]+). The patterns of isotope ions of M+ were also monitored to confirm the assignment of the molecular formulas. HPLC-MS2 analysis was further conducted for elucidating their molecular structures. Twenty-seven different anthocyanins were tentatively identified in the sweet potatoes. Six of them are the first reported in sweet potatoes roots. The quantity and profiles of anthocyanins in sweet potatoes varied greatly with variety. Borami was found, for the first time, to be a rare sweet potato variety with an exceptionally high quantity of pelargonidin-based anthocyanins.</description><subject>anthocyanins</subject><subject>Anthocyanins - analysis</subject><subject>Anthocyanins - chemistry</subject><subject>Anthocyanins - isolation & purification</subject><subject>chemical structure</subject><subject>Chromatography, High Pressure Liquid</subject><subject>high performance liquid chromatography</subject><subject>ions</subject><subject>Ipomoea batatas - chemistry</subject><subject>isotopes</subject><subject>Mass Spectrometry</subject><subject>Plant Tubers - chemistry</subject><subject>quantitative analysis</subject><subject>Republic of Korea</subject><subject>roots</subject><subject>Species Specificity</subject><subject>sweet potatoes</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0MtO3DAUBmALtYIp7aIv0HpTqSxSfM1lOQoMoA5iUGBtHRK7k1HGHmyn1fQR-tQ1hLLqyrL_z-dIP0IfKflGCaOnG8OJlELKAzSjkpFMUlq-QTOSwqyUOT1C70LYEEJKWZBDdMS4yEXJ-Az9qdfgoY3a978h9s5isB2-HcHGPk4PzuC5jWvX7sH2NuDe4tXod4POFoMOa93h5pfWEa9c-uB0wPU4xP4nxJQk-915Dfhhjy9Xyzo7m589b3i-nDdX2e3dzSK7bk6vm_forYEh6A8v5zG6X5zf1ZfZ8ubiqp4vM-C8ihmvBORVx40oOpYXphACKs3zVmrJCjCG6YpzycsiTwnrGHSi0LzsoAKRM8OP0ddp7s67x1GHqLZ9aPUwgNVuDIpyRjkXJSGJnky09S4Er43a-X4Lfq8oUU_Vq9fqk_30MnZ82OruVf7rOoHPEzDgFPzwfVD3DSNUEEIZqYon8WUS0Aa1caO3qYf_rPoLt1yRWg</recordid><startdate>20130327</startdate><enddate>20130327</enddate><creator>Lee, Mi Jin</creator><creator>Park, Jeong Seob</creator><creator>Choi, Dong Seong</creator><creator>Jung, Mun Yhung</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130327</creationdate><title>Characterization and Quantitation of Anthocyanins in Purple-Fleshed Sweet Potatoes Cultivated in Korea by HPLC-DAD and HPLC-ESI-QTOF-MS/MS</title><author>Lee, Mi Jin ; Park, Jeong Seob ; Choi, Dong Seong ; Jung, Mun Yhung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a339t-394a69d3f47d267f744a9e36c5e527aff2e9335387644a2d2ad47e38da9a462f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>anthocyanins</topic><topic>Anthocyanins - analysis</topic><topic>Anthocyanins - chemistry</topic><topic>Anthocyanins - isolation & purification</topic><topic>chemical structure</topic><topic>Chromatography, High Pressure Liquid</topic><topic>high performance liquid chromatography</topic><topic>ions</topic><topic>Ipomoea batatas - chemistry</topic><topic>isotopes</topic><topic>Mass Spectrometry</topic><topic>Plant Tubers - chemistry</topic><topic>quantitative analysis</topic><topic>Republic of Korea</topic><topic>roots</topic><topic>Species Specificity</topic><topic>sweet potatoes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Mi Jin</creatorcontrib><creatorcontrib>Park, Jeong Seob</creatorcontrib><creatorcontrib>Choi, Dong Seong</creatorcontrib><creatorcontrib>Jung, Mun Yhung</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Mi Jin</au><au>Park, Jeong Seob</au><au>Choi, Dong Seong</au><au>Jung, Mun Yhung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and Quantitation of Anthocyanins in Purple-Fleshed Sweet Potatoes Cultivated in Korea by HPLC-DAD and HPLC-ESI-QTOF-MS/MS</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2013-03-27</date><risdate>2013</risdate><volume>61</volume><issue>12</issue><spage>3148</spage><epage>3158</epage><pages>3148-3158</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><abstract>The characterization and quantitative analysis of anthocyanins in four purple-fleshed sweet potato varieties (Borami, Mokpo 62, Shinzami, and Zami) cultivated in Korea were carried out by HPLC/diode array detector (DAD), HPLC-TOF/MS, and HPLC-MS/MS analyses. For the identification of anthocyanins, molecular formulas were first calculated by using the exact mass data of the molecular ions ([M]+). The patterns of isotope ions of M+ were also monitored to confirm the assignment of the molecular formulas. HPLC-MS2 analysis was further conducted for elucidating their molecular structures. Twenty-seven different anthocyanins were tentatively identified in the sweet potatoes. Six of them are the first reported in sweet potatoes roots. The quantity and profiles of anthocyanins in sweet potatoes varied greatly with variety. Borami was found, for the first time, to be a rare sweet potato variety with an exceptionally high quantity of pelargonidin-based anthocyanins.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>23464823</pmid><doi>10.1021/jf3055455</doi><tpages>11</tpages></addata></record> |
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| ispartof | Journal of agricultural and food chemistry, 2013-03, Vol.61 (12), p.3148-3158 |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | anthocyanins Anthocyanins - analysis Anthocyanins - chemistry Anthocyanins - isolation & purification chemical structure Chromatography, High Pressure Liquid high performance liquid chromatography ions Ipomoea batatas - chemistry isotopes Mass Spectrometry Plant Tubers - chemistry quantitative analysis Republic of Korea roots Species Specificity sweet potatoes |
| title | Characterization and Quantitation of Anthocyanins in Purple-Fleshed Sweet Potatoes Cultivated in Korea by HPLC-DAD and HPLC-ESI-QTOF-MS/MS |
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