In vitro electroretinogram for the study of the functionality of differentiated retinal pigment epithelium cells
The purpose of this study is to develop and test a method to reveal if the retinal pigment epithelium (RPE) cells differentiated from human embryonic stem cells (hESC) support the functions of photoreceptors. hESC-derived RPE (hESC–RPE) cells offer a potent cell source for cell replacement therapy t...
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| Veröffentlicht in: | Medical & biological engineering & computing 2013-02, Vol.51 (1-2), p.61-70 |
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| creator | Onnela, Niina Lehtonen, Leena Koski, Mikko Hyttinen, Jari |
| description | The purpose of this study is to develop and test a method to reveal if the retinal pigment epithelium (RPE) cells differentiated from human embryonic stem cells (hESC) support the functions of photoreceptors. hESC-derived RPE (hESC–RPE) cells offer a potent cell source for cell replacement therapy that may be used to prevent certain eye diseases. Methods to assure the functionality of the RPE cells are well warranted. Electroretinograms (ERG) measure the electrophysiological response of the retina to light stimuli. A setup was developed that enables the measurement of ERG in vitro from mice retinas cultured together with hESC–RPE cells. The co-culture of RPE and retinas seems to be a viable tool to assess the functionality of RPE in vitro. However, owing to limited sample size results were somewhat mixed, and thus it was not possible to prove that hESC–RPE cells enhance the ERG response of a mouse retina in vitro. The long-term culturing of the retinas needs to be refined to acquire more conclusive evidence of the supporting role of the RPE and to explore the full potential of the co-culture and ERG methods in assessing RPE functionality. |
| doi_str_mv | 10.1007/s11517-012-0968-7 |
| format | Article |
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Methods to assure the functionality of the RPE cells are well warranted. Electroretinograms (ERG) measure the electrophysiological response of the retina to light stimuli. A setup was developed that enables the measurement of ERG in vitro from mice retinas cultured together with hESC–RPE cells. The co-culture of RPE and retinas seems to be a viable tool to assess the functionality of RPE in vitro. However, owing to limited sample size results were somewhat mixed, and thus it was not possible to prove that hESC–RPE cells enhance the ERG response of a mouse retina in vitro. The long-term culturing of the retinas needs to be refined to acquire more conclusive evidence of the supporting role of the RPE and to explore the full potential of the co-culture and ERG methods in assessing RPE functionality.</description><identifier>ISSN: 0140-0118</identifier><identifier>EISSN: 1741-0444</identifier><identifier>DOI: 10.1007/s11517-012-0968-7</identifier><identifier>PMID: 23065627</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Analysis ; Animals ; Biomedical and Life Sciences ; Biomedical Engineering and Bioengineering ; Biomedicine ; Cell Differentiation ; Cell Line ; Cellular biology ; Clinical trials ; Coculture Techniques ; Computer Applications ; Cornea ; Electrodes ; Electroretinography - methods ; Embryonic Stem Cells - cytology ; Embryonic Stem Cells - metabolism ; Epithelial Cells - cytology ; Epithelial Cells - metabolism ; Eye diseases ; Growth factors ; Human Physiology ; Humans ; Imaging ; Light ; Macular degeneration ; Mice ; Mice, Inbred C57BL ; Morphology ; Original Article ; Photoreceptors ; Radiology ; Retina ; Retinal Pigment Epithelium - cytology ; Stem cells ; Studies ; Transplants & implants</subject><ispartof>Medical & biological engineering & computing, 2013-02, Vol.51 (1-2), p.61-70</ispartof><rights>International Federation for Medical and Biological Engineering 2012</rights><rights>International Federation for Medical and Biological Engineering 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c357t-404deea83bf2cfa8148eaa34c1d6bafe7f125a898cbd93f2f5dbe563e0de78b53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11517-012-0968-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11517-012-0968-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23065627$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Onnela, Niina</creatorcontrib><creatorcontrib>Lehtonen, Leena</creatorcontrib><creatorcontrib>Koski, Mikko</creatorcontrib><creatorcontrib>Hyttinen, Jari</creatorcontrib><title>In vitro electroretinogram for the study of the functionality of differentiated retinal pigment epithelium cells</title><title>Medical & biological engineering & computing</title><addtitle>Med Biol Eng Comput</addtitle><addtitle>Med Biol Eng Comput</addtitle><description>The purpose of this study is to develop and test a method to reveal if the retinal pigment epithelium (RPE) cells differentiated from human embryonic stem cells (hESC) support the functions of photoreceptors. hESC-derived RPE (hESC–RPE) cells offer a potent cell source for cell replacement therapy that may be used to prevent certain eye diseases. Methods to assure the functionality of the RPE cells are well warranted. Electroretinograms (ERG) measure the electrophysiological response of the retina to light stimuli. A setup was developed that enables the measurement of ERG in vitro from mice retinas cultured together with hESC–RPE cells. The co-culture of RPE and retinas seems to be a viable tool to assess the functionality of RPE in vitro. However, owing to limited sample size results were somewhat mixed, and thus it was not possible to prove that hESC–RPE cells enhance the ERG response of a mouse retina in vitro. The long-term culturing of the retinas needs to be refined to acquire more conclusive evidence of the supporting role of the RPE and to explore the full potential of the co-culture and ERG methods in assessing RPE functionality.</description><subject>Analysis</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biomedicine</subject><subject>Cell Differentiation</subject><subject>Cell Line</subject><subject>Cellular biology</subject><subject>Clinical trials</subject><subject>Coculture Techniques</subject><subject>Computer Applications</subject><subject>Cornea</subject><subject>Electrodes</subject><subject>Electroretinography - methods</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Embryonic Stem Cells - metabolism</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - metabolism</subject><subject>Eye diseases</subject><subject>Growth 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| subjects | Analysis Animals Biomedical and Life Sciences Biomedical Engineering and Bioengineering Biomedicine Cell Differentiation Cell Line Cellular biology Clinical trials Coculture Techniques Computer Applications Cornea Electrodes Electroretinography - methods Embryonic Stem Cells - cytology Embryonic Stem Cells - metabolism Epithelial Cells - cytology Epithelial Cells - metabolism Eye diseases Growth factors Human Physiology Humans Imaging Light Macular degeneration Mice Mice, Inbred C57BL Morphology Original Article Photoreceptors Radiology Retina Retinal Pigment Epithelium - cytology Stem cells Studies Transplants & implants |
| title | In vitro electroretinogram for the study of the functionality of differentiated retinal pigment epithelium cells |
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