Expression of P2X5 receptors in the rat, cat, mouse and guinea pig dorsal root ganglion
P2X receptors are ATP-gated cationic channels composed of seven cloned subunits (P2X 1 –7 ). P2X 3 homomultimer and P2X 2/3 heteromultimer receptors expressed by primary afferent dorsal root ganglion (DRG) neurons are involved in pain processing. The aim of the study was to investigate the expressio...
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| Veröffentlicht in: | Histochemistry and cell biology 2013-04, Vol.139 (4), p.549-557 |
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| creator | Zeng, Jun-Wei Cheng, Sai-Yu Liu, Xiao-Hong Zhao, Yan-Dong Xiao, Zhi Burnstock, Geoffrey Ruan, Huai-Zhen |
| description | P2X receptors are ATP-gated cationic channels composed of seven cloned subunits (P2X
1
–7
). P2X
3
homomultimer and P2X
2/3
heteromultimer receptors expressed by primary afferent dorsal root ganglion (DRG) neurons are involved in pain processing. The aim of the study was to investigate the expression of the P2X
5
receptor subunit in DRG in different species including mouse, rat, cat and guinea pig. Immunohistochemistry showed that P2X
5
receptors exhibited low levels of immunostaining in rat DRG, but high levels in mouse and guinea pig. Only a few neurons were immunoreactive for P2X
5
receptors in cat. In mouse DRG, the P2X
5
receptor was expressed largely by medium-diameter neurons (42.9 %), less in small (29.3 %) and large (27.8 %) neurons. In contrast, in the guinea pig DRG, P2X
5
receptor expression was greatest in small-diameter (42.6 %), less in medium- (36.3 %) and large-diameter (21.1 %) neurons. Colocalization experiments revealed that, in mouse DRG, 65.5, 10.9 and 27.1 % of P2X
5
receptors were immunoreactive for NF-200, CGRP and calbindin, while only a few P2X
5
-immunoreactive (IR) neurons were coexpressed with IB4 or with NOS. In guinea pig DRG, a total of 60.5 and 40.5 % of P2X
5
-IR neurons were coexpressed with IB4 or with CGRP, while 20.3 and 24.5 % of P2X
5
receptors were coexpressed with NF-200 or with NOS. Only a few P2X
5
-IR neurons were coexpressed with calbindin in guinea pig DRG. It will be of great interest to clarify the relative physiological and pathophysiological roles of P2X
5
receptors. |
| doi_str_mv | 10.1007/s00418-012-1046-9 |
| format | Article |
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1
–7
). P2X
3
homomultimer and P2X
2/3
heteromultimer receptors expressed by primary afferent dorsal root ganglion (DRG) neurons are involved in pain processing. The aim of the study was to investigate the expression of the P2X
5
receptor subunit in DRG in different species including mouse, rat, cat and guinea pig. Immunohistochemistry showed that P2X
5
receptors exhibited low levels of immunostaining in rat DRG, but high levels in mouse and guinea pig. Only a few neurons were immunoreactive for P2X
5
receptors in cat. In mouse DRG, the P2X
5
receptor was expressed largely by medium-diameter neurons (42.9 %), less in small (29.3 %) and large (27.8 %) neurons. In contrast, in the guinea pig DRG, P2X
5
receptor expression was greatest in small-diameter (42.6 %), less in medium- (36.3 %) and large-diameter (21.1 %) neurons. Colocalization experiments revealed that, in mouse DRG, 65.5, 10.9 and 27.1 % of P2X
5
receptors were immunoreactive for NF-200, CGRP and calbindin, while only a few P2X
5
-immunoreactive (IR) neurons were coexpressed with IB4 or with NOS. In guinea pig DRG, a total of 60.5 and 40.5 % of P2X
5
-IR neurons were coexpressed with IB4 or with CGRP, while 20.3 and 24.5 % of P2X
5
receptors were coexpressed with NF-200 or with NOS. Only a few P2X
5
-IR neurons were coexpressed with calbindin in guinea pig DRG. It will be of great interest to clarify the relative physiological and pathophysiological roles of P2X
5
receptors.</description><identifier>ISSN: 0948-6143</identifier><identifier>EISSN: 1432-119X</identifier><identifier>DOI: 10.1007/s00418-012-1046-9</identifier><identifier>PMID: 23160624</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Adenosine Triphosphate - metabolism ; Animals ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Calbindins ; Calcitonin Gene-Related Peptide - metabolism ; Cats ; Cell Biology ; Developmental Biology ; Ganglia, Spinal - metabolism ; Guinea Pigs ; Immunohistochemistry ; Mice ; Neurons, Afferent - metabolism ; Original Paper ; Rats ; Rats, Sprague-Dawley ; Receptors, Purinergic P2X5 - biosynthesis ; Receptors, Purinergic P2X5 - metabolism ; S100 Calcium Binding Protein G - biosynthesis</subject><ispartof>Histochemistry and cell biology, 2013-04, Vol.139 (4), p.549-557</ispartof><rights>Springer-Verlag Berlin Heidelberg 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3259-33ddeba85a8297a09bd4aee25ca4e502ecb2ce7f226bd3b209ec1d89e823d56a3</citedby><cites>FETCH-LOGICAL-c3259-33ddeba85a8297a09bd4aee25ca4e502ecb2ce7f226bd3b209ec1d89e823d56a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00418-012-1046-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00418-012-1046-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23160624$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zeng, Jun-Wei</creatorcontrib><creatorcontrib>Cheng, Sai-Yu</creatorcontrib><creatorcontrib>Liu, Xiao-Hong</creatorcontrib><creatorcontrib>Zhao, Yan-Dong</creatorcontrib><creatorcontrib>Xiao, Zhi</creatorcontrib><creatorcontrib>Burnstock, Geoffrey</creatorcontrib><creatorcontrib>Ruan, Huai-Zhen</creatorcontrib><title>Expression of P2X5 receptors in the rat, cat, mouse and guinea pig dorsal root ganglion</title><title>Histochemistry and cell biology</title><addtitle>Histochem Cell Biol</addtitle><addtitle>Histochem Cell Biol</addtitle><description>P2X receptors are ATP-gated cationic channels composed of seven cloned subunits (P2X
1
–7
). P2X
3
homomultimer and P2X
2/3
heteromultimer receptors expressed by primary afferent dorsal root ganglion (DRG) neurons are involved in pain processing. The aim of the study was to investigate the expression of the P2X
5
receptor subunit in DRG in different species including mouse, rat, cat and guinea pig. Immunohistochemistry showed that P2X
5
receptors exhibited low levels of immunostaining in rat DRG, but high levels in mouse and guinea pig. Only a few neurons were immunoreactive for P2X
5
receptors in cat. In mouse DRG, the P2X
5
receptor was expressed largely by medium-diameter neurons (42.9 %), less in small (29.3 %) and large (27.8 %) neurons. In contrast, in the guinea pig DRG, P2X
5
receptor expression was greatest in small-diameter (42.6 %), less in medium- (36.3 %) and large-diameter (21.1 %) neurons. Colocalization experiments revealed that, in mouse DRG, 65.5, 10.9 and 27.1 % of P2X
5
receptors were immunoreactive for NF-200, CGRP and calbindin, while only a few P2X
5
-immunoreactive (IR) neurons were coexpressed with IB4 or with NOS. In guinea pig DRG, a total of 60.5 and 40.5 % of P2X
5
-IR neurons were coexpressed with IB4 or with CGRP, while 20.3 and 24.5 % of P2X
5
receptors were coexpressed with NF-200 or with NOS. Only a few P2X
5
-IR neurons were coexpressed with calbindin in guinea pig DRG. It will be of great interest to clarify the relative physiological and pathophysiological roles of P2X
5
receptors.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Calbindins</subject><subject>Calcitonin Gene-Related Peptide - metabolism</subject><subject>Cats</subject><subject>Cell Biology</subject><subject>Developmental Biology</subject><subject>Ganglia, Spinal - metabolism</subject><subject>Guinea Pigs</subject><subject>Immunohistochemistry</subject><subject>Mice</subject><subject>Neurons, Afferent - metabolism</subject><subject>Original Paper</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, Purinergic P2X5 - biosynthesis</subject><subject>Receptors, Purinergic P2X5 - metabolism</subject><subject>S100 Calcium Binding Protein G - biosynthesis</subject><issn>0948-6143</issn><issn>1432-119X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD1PwzAQhi0EoqXwA1iQRwYC_ojTeERV-ZCQYADRzXLsS0iVxMFOJPj3uEphZPHJvudenR-Ezim5poQsbwIhKc0TQllCSZol8gDNacrjjcrNIZoTmeZJFl9m6CSELSFUSMaO0YxxmpGMpXP0vv7qPYRQuw67Er-wjcAeDPSD8wHXHR4-AHs9XGGzO1o3BsC6s7ga6w407usK24jqBnvnBlzprmpi2Ck6KnUT4GxfF-jtbv26ekienu8fV7dPieFMyIRza6HQudA5k0tNZGFTDcCE0SkIwsAUzMCyZCwrLC8YkWCozSXkjFuRab5Al1Nu793nCGFQbR0MNI3uIO6qKKd5JqMmEVE6oca7EDyUqvd1q_23okTtfKrJp4o-1c6nknHmYh8_Fi3Yv4lfgRFgExBiq6vAq60bfRe__E_qD_O6f_4</recordid><startdate>201304</startdate><enddate>201304</enddate><creator>Zeng, Jun-Wei</creator><creator>Cheng, Sai-Yu</creator><creator>Liu, Xiao-Hong</creator><creator>Zhao, Yan-Dong</creator><creator>Xiao, Zhi</creator><creator>Burnstock, Geoffrey</creator><creator>Ruan, Huai-Zhen</creator><general>Springer-Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201304</creationdate><title>Expression of P2X5 receptors in the rat, cat, mouse and guinea pig dorsal root ganglion</title><author>Zeng, Jun-Wei ; Cheng, Sai-Yu ; Liu, Xiao-Hong ; Zhao, Yan-Dong ; Xiao, Zhi ; Burnstock, Geoffrey ; Ruan, Huai-Zhen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3259-33ddeba85a8297a09bd4aee25ca4e502ecb2ce7f226bd3b209ec1d89e823d56a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Animals</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Calbindins</topic><topic>Calcitonin Gene-Related Peptide - metabolism</topic><topic>Cats</topic><topic>Cell Biology</topic><topic>Developmental Biology</topic><topic>Ganglia, Spinal - metabolism</topic><topic>Guinea Pigs</topic><topic>Immunohistochemistry</topic><topic>Mice</topic><topic>Neurons, Afferent - metabolism</topic><topic>Original Paper</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, Purinergic P2X5 - biosynthesis</topic><topic>Receptors, Purinergic P2X5 - metabolism</topic><topic>S100 Calcium Binding Protein G - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zeng, Jun-Wei</creatorcontrib><creatorcontrib>Cheng, Sai-Yu</creatorcontrib><creatorcontrib>Liu, Xiao-Hong</creatorcontrib><creatorcontrib>Zhao, Yan-Dong</creatorcontrib><creatorcontrib>Xiao, Zhi</creatorcontrib><creatorcontrib>Burnstock, Geoffrey</creatorcontrib><creatorcontrib>Ruan, Huai-Zhen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Histochemistry and cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zeng, Jun-Wei</au><au>Cheng, Sai-Yu</au><au>Liu, Xiao-Hong</au><au>Zhao, Yan-Dong</au><au>Xiao, Zhi</au><au>Burnstock, Geoffrey</au><au>Ruan, Huai-Zhen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of P2X5 receptors in the rat, cat, mouse and guinea pig dorsal root ganglion</atitle><jtitle>Histochemistry and cell biology</jtitle><stitle>Histochem Cell Biol</stitle><addtitle>Histochem Cell Biol</addtitle><date>2013-04</date><risdate>2013</risdate><volume>139</volume><issue>4</issue><spage>549</spage><epage>557</epage><pages>549-557</pages><issn>0948-6143</issn><eissn>1432-119X</eissn><abstract>P2X receptors are ATP-gated cationic channels composed of seven cloned subunits (P2X
1
–7
). P2X
3
homomultimer and P2X
2/3
heteromultimer receptors expressed by primary afferent dorsal root ganglion (DRG) neurons are involved in pain processing. The aim of the study was to investigate the expression of the P2X
5
receptor subunit in DRG in different species including mouse, rat, cat and guinea pig. Immunohistochemistry showed that P2X
5
receptors exhibited low levels of immunostaining in rat DRG, but high levels in mouse and guinea pig. Only a few neurons were immunoreactive for P2X
5
receptors in cat. In mouse DRG, the P2X
5
receptor was expressed largely by medium-diameter neurons (42.9 %), less in small (29.3 %) and large (27.8 %) neurons. In contrast, in the guinea pig DRG, P2X
5
receptor expression was greatest in small-diameter (42.6 %), less in medium- (36.3 %) and large-diameter (21.1 %) neurons. Colocalization experiments revealed that, in mouse DRG, 65.5, 10.9 and 27.1 % of P2X
5
receptors were immunoreactive for NF-200, CGRP and calbindin, while only a few P2X
5
-immunoreactive (IR) neurons were coexpressed with IB4 or with NOS. In guinea pig DRG, a total of 60.5 and 40.5 % of P2X
5
-IR neurons were coexpressed with IB4 or with CGRP, while 20.3 and 24.5 % of P2X
5
receptors were coexpressed with NF-200 or with NOS. Only a few P2X
5
-IR neurons were coexpressed with calbindin in guinea pig DRG. It will be of great interest to clarify the relative physiological and pathophysiological roles of P2X
5
receptors.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>23160624</pmid><doi>10.1007/s00418-012-1046-9</doi><tpages>9</tpages></addata></record> |
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| ispartof | Histochemistry and cell biology, 2013-04, Vol.139 (4), p.549-557 |
| issn | 0948-6143 1432-119X |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Adenosine Triphosphate - metabolism Animals Biochemistry Biomedical and Life Sciences Biomedicine Calbindins Calcitonin Gene-Related Peptide - metabolism Cats Cell Biology Developmental Biology Ganglia, Spinal - metabolism Guinea Pigs Immunohistochemistry Mice Neurons, Afferent - metabolism Original Paper Rats Rats, Sprague-Dawley Receptors, Purinergic P2X5 - biosynthesis Receptors, Purinergic P2X5 - metabolism S100 Calcium Binding Protein G - biosynthesis |
| title | Expression of P2X5 receptors in the rat, cat, mouse and guinea pig dorsal root ganglion |
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