TAT hitchhiker selection expanded to folding helpers, multimeric interactions and combinations with protein fragment complementation

The twin-arginine translocation (TAT) pathway of the bacterial cytoplasmic membrane mediates translocation only of proteins that accomplished a native-like conformation. We deploy this feature in modular selection systems for directed evolution, in which folding helpers as well as dimeric or oligome...

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Veröffentlicht in:	Protein engineering, design and selection design and selection, 2013-03, Vol.26 (3), p.225-242
Hauptverfasser:	Speck, Janina, Räuber, Christina, Kükenshöner, Tim, Niemöller, Christoph, Mueller, Katelyn J., Schleberger, Paula, Dondapati, Padmarupa, Hecky, Jochen, Arndt, Katja M., Müller, Kristian M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Arginine - genetics Arginine - metabolism beta -Lactamase beta-Lactamases - chemistry beta-Lactamases - genetics beta-Lactamases - metabolism Cloning, Molecular - methods Cytoplasmic membranes directed evolution Enhancers Escherichia coli - chemistry Escherichia coli - genetics Escherichia coli - metabolism Escherichia coli Proteins - chemistry Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Fitness Membrane Transport Proteins - chemistry Membrane Transport Proteins - genetics Membrane Transport Proteins - metabolism Microphthalmia-associated transcription factor Models, Molecular Protein Engineering - methods Protein Folding Protein interaction Protein Interaction Mapping Protein Multimerization Protein Transport Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Translocation
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