Global gene expression differentiating pure bronchioloalveolar carcinoma from adenocarcinoma with bronchioloalveolar carcinoma features
OBJECTIVES Pure bronchioloalveolar carcinoma (BAC) is considered the early stage of lung adenocarcinoma, and is even regarded as lung adenocarcinoma in situ. This study was designed to investigate the differences in the gene expression of pure BAC and that of adenocarcinoma with bronchioloalveolar f...
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| Veröffentlicht in: | European journal of cardio-thoracic surgery 2013-04, Vol.43 (4), p.765-771 |
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| container_title | European journal of cardio-thoracic surgery |
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| creator | Zhang, Wen-Cheng Zhang, Zhen-Fa You, Jian Wang, Chang-Li |
| description | OBJECTIVES
Pure bronchioloalveolar carcinoma (BAC) is considered the early stage of lung adenocarcinoma, and is even regarded as lung adenocarcinoma in situ. This study was designed to investigate the differences in the gene expression of pure BAC and that of adenocarcinoma with bronchioloalveolar features and explore the mechanism of BAC progression to adenocarcinoma with bronchioloalveolar features
METHODS
Total RNA was extracted from 16 tissue specimens. Expression analysis was carried out using Agilent 4 × 44 k arrays. Gene ontology analysis was used to define pathways altered in bronchioloalveolar progression. Differentially expressed candidate genes were validated using quantitative real-time PCR. The statistical analysis was carried out according to the methods of the paired t-test.
RESULTS
Adenocarcinoma with bronchioloalveolar features demonstrated an increased expression of 23 genes and reduced expression of 20 genes compared with BAC. These genes were considered candidate marker genes for tumour progression and metastasis. Genes overexpressed in adenocarcinoma with bronchioloalveolar features included fibroblast growth factor receptor 1, and CLDN18 (claudin 18), whereas those overexpressed in BAC included ataxia telangiectasia and Rad3 related (ataxia telangiectasia mutated and Rad3-related), and activating transcription factor 2. Mitogen-activated protein kinase (MAPK) pathway seemed dysregulated in adenocarcinoma with bronchioloalveolar features compared with pure BAC.
CONCLUSIONS
Microarray-based expression profiling revealed interesting novel candidate genes in BAC and adenocarcinoma with bronchioloalveolar features. The MAPK pathway seemed dysregulated in adenocarcinoma with bronchioloalveolar features compared with the pure BAC pathway, which is worthy of being explored because it could partially explain the mechanism of the progression of BAC to adenocarcinoma with bronchioloalveolar features. |
| doi_str_mv | 10.1093/ejcts/ezs427 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1318093338</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/ejcts/ezs427</oup_id><sourcerecordid>1318093338</sourcerecordid><originalsourceid>FETCH-LOGICAL-c318t-f297cfda7973248186b828be4100125ba72e3a9322857e9b2b76417fc74e4a013</originalsourceid><addsrcrecordid>eNqFkD1PwzAQhi0EoqWwMSNvMBDqr8bOiCooSEgsILFFjnNpXSVxsBO-_gB_m0AKbDDd6fTce7oHoUNKzihJ-BTWpg1TeAuCyS00pkrySHLxsN33hJJIJoKM0F4Ia0JIzJncRSPGVCykUmP0vihdpku8hBowvDQeQrCuxrktCvBQt1a3tl7ipvOAM-9qs7KudLp8Aldqj432xtau0rjwrsI6h9r9zp5tu_pnC3TbR4d9tFPoMsDBpk7Q_eXF3fwqurldXM_PbyLDqWqjgiXSFLmWieRMKKriTDGVgaCEUDbLtGTAdcL7B2cSkoxlMhZUFkYKEJpQPkEnQ27j3WMHoU0rGwyUpa7BdSGl_ZneKueqR08H1HgXgocibbyttH9NKUk_1adf6tNBfY8fbZK7rIL8B_523QPHA-C65u-oD1_Nko0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1318093338</pqid></control><display><type>article</type><title>Global gene expression differentiating pure bronchioloalveolar carcinoma from adenocarcinoma with bronchioloalveolar carcinoma features</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Zhang, Wen-Cheng ; Zhang, Zhen-Fa ; You, Jian ; Wang, Chang-Li</creator><creatorcontrib>Zhang, Wen-Cheng ; Zhang, Zhen-Fa ; You, Jian ; Wang, Chang-Li</creatorcontrib><description>OBJECTIVES
Pure bronchioloalveolar carcinoma (BAC) is considered the early stage of lung adenocarcinoma, and is even regarded as lung adenocarcinoma in situ. This study was designed to investigate the differences in the gene expression of pure BAC and that of adenocarcinoma with bronchioloalveolar features and explore the mechanism of BAC progression to adenocarcinoma with bronchioloalveolar features
METHODS
Total RNA was extracted from 16 tissue specimens. Expression analysis was carried out using Agilent 4 × 44 k arrays. Gene ontology analysis was used to define pathways altered in bronchioloalveolar progression. Differentially expressed candidate genes were validated using quantitative real-time PCR. The statistical analysis was carried out according to the methods of the paired t-test.
RESULTS
Adenocarcinoma with bronchioloalveolar features demonstrated an increased expression of 23 genes and reduced expression of 20 genes compared with BAC. These genes were considered candidate marker genes for tumour progression and metastasis. Genes overexpressed in adenocarcinoma with bronchioloalveolar features included fibroblast growth factor receptor 1, and CLDN18 (claudin 18), whereas those overexpressed in BAC included ataxia telangiectasia and Rad3 related (ataxia telangiectasia mutated and Rad3-related), and activating transcription factor 2. Mitogen-activated protein kinase (MAPK) pathway seemed dysregulated in adenocarcinoma with bronchioloalveolar features compared with pure BAC.
CONCLUSIONS
Microarray-based expression profiling revealed interesting novel candidate genes in BAC and adenocarcinoma with bronchioloalveolar features. The MAPK pathway seemed dysregulated in adenocarcinoma with bronchioloalveolar features compared with the pure BAC pathway, which is worthy of being explored because it could partially explain the mechanism of the progression of BAC to adenocarcinoma with bronchioloalveolar features.</description><identifier>ISSN: 1010-7940</identifier><identifier>EISSN: 1873-734X</identifier><identifier>DOI: 10.1093/ejcts/ezs427</identifier><identifier>PMID: 22864788</identifier><language>eng</language><publisher>Germany: Oxford University Press</publisher><subject>Adenocarcinoma - chemistry ; Adenocarcinoma - classification ; Adenocarcinoma - diagnosis ; Adenocarcinoma - metabolism ; Adenocarcinoma of Lung ; Adenocarcinoma, Bronchiolo-Alveolar - classification ; Adenocarcinoma, Bronchiolo-Alveolar - diagnosis ; Adenocarcinoma, Bronchiolo-Alveolar - genetics ; Adenocarcinoma, Bronchiolo-Alveolar - metabolism ; Aged ; Aged, 80 and over ; Diagnosis, Differential ; Female ; Gene Expression Profiling ; Humans ; Lung Neoplasms - chemistry ; Lung Neoplasms - classification ; Lung Neoplasms - diagnosis ; Lung Neoplasms - genetics ; Lung Neoplasms - metabolism ; Male ; Middle Aged ; Neoplasm Proteins - biosynthesis ; Neoplasm Proteins - genetics ; Neoplasm Staging ; Oligonucleotide Array Sequence Analysis ; Real-Time Polymerase Chain Reaction ; RNA, Messenger - analysis ; RNA, Messenger - metabolism</subject><ispartof>European journal of cardio-thoracic surgery, 2013-04, Vol.43 (4), p.765-771</ispartof><rights>The Author 2012. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c318t-f297cfda7973248186b828be4100125ba72e3a9322857e9b2b76417fc74e4a013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22864788$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Wen-Cheng</creatorcontrib><creatorcontrib>Zhang, Zhen-Fa</creatorcontrib><creatorcontrib>You, Jian</creatorcontrib><creatorcontrib>Wang, Chang-Li</creatorcontrib><title>Global gene expression differentiating pure bronchioloalveolar carcinoma from adenocarcinoma with bronchioloalveolar carcinoma features</title><title>European journal of cardio-thoracic surgery</title><addtitle>Eur J Cardiothorac Surg</addtitle><description>OBJECTIVES
Pure bronchioloalveolar carcinoma (BAC) is considered the early stage of lung adenocarcinoma, and is even regarded as lung adenocarcinoma in situ. This study was designed to investigate the differences in the gene expression of pure BAC and that of adenocarcinoma with bronchioloalveolar features and explore the mechanism of BAC progression to adenocarcinoma with bronchioloalveolar features
METHODS
Total RNA was extracted from 16 tissue specimens. Expression analysis was carried out using Agilent 4 × 44 k arrays. Gene ontology analysis was used to define pathways altered in bronchioloalveolar progression. Differentially expressed candidate genes were validated using quantitative real-time PCR. The statistical analysis was carried out according to the methods of the paired t-test.
RESULTS
Adenocarcinoma with bronchioloalveolar features demonstrated an increased expression of 23 genes and reduced expression of 20 genes compared with BAC. These genes were considered candidate marker genes for tumour progression and metastasis. Genes overexpressed in adenocarcinoma with bronchioloalveolar features included fibroblast growth factor receptor 1, and CLDN18 (claudin 18), whereas those overexpressed in BAC included ataxia telangiectasia and Rad3 related (ataxia telangiectasia mutated and Rad3-related), and activating transcription factor 2. Mitogen-activated protein kinase (MAPK) pathway seemed dysregulated in adenocarcinoma with bronchioloalveolar features compared with pure BAC.
CONCLUSIONS
Microarray-based expression profiling revealed interesting novel candidate genes in BAC and adenocarcinoma with bronchioloalveolar features. The MAPK pathway seemed dysregulated in adenocarcinoma with bronchioloalveolar features compared with the pure BAC pathway, which is worthy of being explored because it could partially explain the mechanism of the progression of BAC to adenocarcinoma with bronchioloalveolar features.</description><subject>Adenocarcinoma - chemistry</subject><subject>Adenocarcinoma - classification</subject><subject>Adenocarcinoma - diagnosis</subject><subject>Adenocarcinoma - metabolism</subject><subject>Adenocarcinoma of Lung</subject><subject>Adenocarcinoma, Bronchiolo-Alveolar - classification</subject><subject>Adenocarcinoma, Bronchiolo-Alveolar - diagnosis</subject><subject>Adenocarcinoma, Bronchiolo-Alveolar - genetics</subject><subject>Adenocarcinoma, Bronchiolo-Alveolar - metabolism</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Diagnosis, Differential</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Humans</subject><subject>Lung Neoplasms - chemistry</subject><subject>Lung Neoplasms - classification</subject><subject>Lung Neoplasms - diagnosis</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - metabolism</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Neoplasm Proteins - biosynthesis</subject><subject>Neoplasm Proteins - genetics</subject><subject>Neoplasm Staging</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - metabolism</subject><issn>1010-7940</issn><issn>1873-734X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQhi0EoqWwMSNvMBDqr8bOiCooSEgsILFFjnNpXSVxsBO-_gB_m0AKbDDd6fTce7oHoUNKzihJ-BTWpg1TeAuCyS00pkrySHLxsN33hJJIJoKM0F4Ia0JIzJncRSPGVCykUmP0vihdpku8hBowvDQeQrCuxrktCvBQt1a3tl7ipvOAM-9qs7KudLp8Aldqj432xtau0rjwrsI6h9r9zp5tu_pnC3TbR4d9tFPoMsDBpk7Q_eXF3fwqurldXM_PbyLDqWqjgiXSFLmWieRMKKriTDGVgaCEUDbLtGTAdcL7B2cSkoxlMhZUFkYKEJpQPkEnQ27j3WMHoU0rGwyUpa7BdSGl_ZneKueqR08H1HgXgocibbyttH9NKUk_1adf6tNBfY8fbZK7rIL8B_523QPHA-C65u-oD1_Nko0</recordid><startdate>201304</startdate><enddate>201304</enddate><creator>Zhang, Wen-Cheng</creator><creator>Zhang, Zhen-Fa</creator><creator>You, Jian</creator><creator>Wang, Chang-Li</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201304</creationdate><title>Global gene expression differentiating pure bronchioloalveolar carcinoma from adenocarcinoma with bronchioloalveolar carcinoma features</title><author>Zhang, Wen-Cheng ; Zhang, Zhen-Fa ; You, Jian ; Wang, Chang-Li</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c318t-f297cfda7973248186b828be4100125ba72e3a9322857e9b2b76417fc74e4a013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adenocarcinoma - chemistry</topic><topic>Adenocarcinoma - classification</topic><topic>Adenocarcinoma - diagnosis</topic><topic>Adenocarcinoma - metabolism</topic><topic>Adenocarcinoma of Lung</topic><topic>Adenocarcinoma, Bronchiolo-Alveolar - classification</topic><topic>Adenocarcinoma, Bronchiolo-Alveolar - diagnosis</topic><topic>Adenocarcinoma, Bronchiolo-Alveolar - genetics</topic><topic>Adenocarcinoma, Bronchiolo-Alveolar - metabolism</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Diagnosis, Differential</topic><topic>Female</topic><topic>Gene Expression Profiling</topic><topic>Humans</topic><topic>Lung Neoplasms - chemistry</topic><topic>Lung Neoplasms - classification</topic><topic>Lung Neoplasms - diagnosis</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - metabolism</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Neoplasm Proteins - biosynthesis</topic><topic>Neoplasm Proteins - genetics</topic><topic>Neoplasm Staging</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Wen-Cheng</creatorcontrib><creatorcontrib>Zhang, Zhen-Fa</creatorcontrib><creatorcontrib>You, Jian</creatorcontrib><creatorcontrib>Wang, Chang-Li</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of cardio-thoracic surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Wen-Cheng</au><au>Zhang, Zhen-Fa</au><au>You, Jian</au><au>Wang, Chang-Li</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Global gene expression differentiating pure bronchioloalveolar carcinoma from adenocarcinoma with bronchioloalveolar carcinoma features</atitle><jtitle>European journal of cardio-thoracic surgery</jtitle><addtitle>Eur J Cardiothorac Surg</addtitle><date>2013-04</date><risdate>2013</risdate><volume>43</volume><issue>4</issue><spage>765</spage><epage>771</epage><pages>765-771</pages><issn>1010-7940</issn><eissn>1873-734X</eissn><abstract>OBJECTIVES
Pure bronchioloalveolar carcinoma (BAC) is considered the early stage of lung adenocarcinoma, and is even regarded as lung adenocarcinoma in situ. This study was designed to investigate the differences in the gene expression of pure BAC and that of adenocarcinoma with bronchioloalveolar features and explore the mechanism of BAC progression to adenocarcinoma with bronchioloalveolar features
METHODS
Total RNA was extracted from 16 tissue specimens. Expression analysis was carried out using Agilent 4 × 44 k arrays. Gene ontology analysis was used to define pathways altered in bronchioloalveolar progression. Differentially expressed candidate genes were validated using quantitative real-time PCR. The statistical analysis was carried out according to the methods of the paired t-test.
RESULTS
Adenocarcinoma with bronchioloalveolar features demonstrated an increased expression of 23 genes and reduced expression of 20 genes compared with BAC. These genes were considered candidate marker genes for tumour progression and metastasis. Genes overexpressed in adenocarcinoma with bronchioloalveolar features included fibroblast growth factor receptor 1, and CLDN18 (claudin 18), whereas those overexpressed in BAC included ataxia telangiectasia and Rad3 related (ataxia telangiectasia mutated and Rad3-related), and activating transcription factor 2. Mitogen-activated protein kinase (MAPK) pathway seemed dysregulated in adenocarcinoma with bronchioloalveolar features compared with pure BAC.
CONCLUSIONS
Microarray-based expression profiling revealed interesting novel candidate genes in BAC and adenocarcinoma with bronchioloalveolar features. The MAPK pathway seemed dysregulated in adenocarcinoma with bronchioloalveolar features compared with the pure BAC pathway, which is worthy of being explored because it could partially explain the mechanism of the progression of BAC to adenocarcinoma with bronchioloalveolar features.</abstract><cop>Germany</cop><pub>Oxford University Press</pub><pmid>22864788</pmid><doi>10.1093/ejcts/ezs427</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Adenocarcinoma - chemistry Adenocarcinoma - classification Adenocarcinoma - diagnosis Adenocarcinoma - metabolism Adenocarcinoma of Lung Adenocarcinoma, Bronchiolo-Alveolar - classification Adenocarcinoma, Bronchiolo-Alveolar - diagnosis Adenocarcinoma, Bronchiolo-Alveolar - genetics Adenocarcinoma, Bronchiolo-Alveolar - metabolism Aged Aged, 80 and over Diagnosis, Differential Female Gene Expression Profiling Humans Lung Neoplasms - chemistry Lung Neoplasms - classification Lung Neoplasms - diagnosis Lung Neoplasms - genetics Lung Neoplasms - metabolism Male Middle Aged Neoplasm Proteins - biosynthesis Neoplasm Proteins - genetics Neoplasm Staging Oligonucleotide Array Sequence Analysis Real-Time Polymerase Chain Reaction RNA, Messenger - analysis RNA, Messenger - metabolism |
| title | Global gene expression differentiating pure bronchioloalveolar carcinoma from adenocarcinoma with bronchioloalveolar carcinoma features |
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