Application of a microfluidic chip-based 3D co-culture to test drug sensitivity for individualized treatment of lung cancer
Abstract Individualized treatment is a promising clinical strategy for lung cancer, and drug sensitivity testing is fundamental to this scheme. We aimed to develop an effective drug sensitivity test platform to support individualized treatment. We designed a microfluidic chip-based, three-dimensiona...
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| Veröffentlicht in: | Biomaterials 2013-05, Vol.34 (16), p.4109-4117 |
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| container_title | Biomaterials |
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| creator | Xu, Zhiyun Gao, Yanghui Hao, Yuanyuan Li, Encheng Wang, Yan Zhang, Jianing Wang, Wenxin Gao, Zhancheng Wang, Qi |
| description | Abstract Individualized treatment is a promising clinical strategy for lung cancer, and drug sensitivity testing is fundamental to this scheme. We aimed to develop an effective drug sensitivity test platform to support individualized treatment. We designed a microfluidic chip-based, three-dimensional (3D) co-culture drug sensitivity test platform. A mono-lung cancer cell line, a mixture of lung cancer and stromal cell lines, and cells from fresh lung cancer tissues were cultured in 3D under continuous media supplementation, mimicking the actual tumor microenvironment in vivo. The cells were treated with anti-cancer drugs according to a gradient concentration generator inside the chips to screen the appropriate chemotherapy schemes. We successfully cultured cell lines or primary cells with this device. We also smoothly assayed the sensitivities of different anti-cancer drugs in parallel and accurately screened appropriate-dose, single and combined-drug chemotherapy schemes for eight patients. Our microfluidic device is a simple, reliable, and high-throughput platform to test drug sensitivity. It would be possible for chemotherapists to screen the appropriate chemotherapy schemes to guide individualized treatment in lung cancer. |
| doi_str_mv | 10.1016/j.biomaterials.2013.02.045 |
| format | Article |
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We aimed to develop an effective drug sensitivity test platform to support individualized treatment. We designed a microfluidic chip-based, three-dimensional (3D) co-culture drug sensitivity test platform. A mono-lung cancer cell line, a mixture of lung cancer and stromal cell lines, and cells from fresh lung cancer tissues were cultured in 3D under continuous media supplementation, mimicking the actual tumor microenvironment in vivo. The cells were treated with anti-cancer drugs according to a gradient concentration generator inside the chips to screen the appropriate chemotherapy schemes. We successfully cultured cell lines or primary cells with this device. We also smoothly assayed the sensitivities of different anti-cancer drugs in parallel and accurately screened appropriate-dose, single and combined-drug chemotherapy schemes for eight patients. Our microfluidic device is a simple, reliable, and high-throughput platform to test drug sensitivity. It would be possible for chemotherapists to screen the appropriate chemotherapy schemes to guide individualized treatment in lung cancer.</description><identifier>ISSN: 0142-9612</identifier><identifier>ISSN: 1878-5905</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2013.02.045</identifier><identifier>PMID: 23473962</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>3D co-culture ; Advanced Basic Science ; Antineoplastic Agents - pharmacology ; Antineoplastic Agents - therapeutic use ; Apoptosis - drug effects ; Cell Line, Tumor ; Cell Shape - drug effects ; Chemotherapy ; Coculture Techniques - methods ; Dentistry ; Drug Screening Assays, Antitumor ; Drug sensitivity test ; Fibroblasts - cytology ; Fibroblasts - drug effects ; Humans ; Individualized treatment ; Lung cancer ; Lung Neoplasms - drug therapy ; Lung Neoplasms - pathology ; Microfluidic Analytical Techniques - methods ; Microfluidic chip ; Reproducibility of Results</subject><ispartof>Biomaterials, 2013-05, Vol.34 (16), p.4109-4117</ispartof><rights>Elsevier Ltd</rights><rights>2013 Elsevier Ltd</rights><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c558t-e575c27f3bdd6b100d4553d4cece4c2cb3952930b6484ca1d02cf8235c91e0133</citedby><cites>FETCH-LOGICAL-c558t-e575c27f3bdd6b100d4553d4cece4c2cb3952930b6484ca1d02cf8235c91e0133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0142961213002287$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23473962$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Zhiyun</creatorcontrib><creatorcontrib>Gao, Yanghui</creatorcontrib><creatorcontrib>Hao, Yuanyuan</creatorcontrib><creatorcontrib>Li, Encheng</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Zhang, Jianing</creatorcontrib><creatorcontrib>Wang, Wenxin</creatorcontrib><creatorcontrib>Gao, Zhancheng</creatorcontrib><creatorcontrib>Wang, Qi</creatorcontrib><title>Application of a microfluidic chip-based 3D co-culture to test drug sensitivity for individualized treatment of lung cancer</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract Individualized treatment is a promising clinical strategy for lung cancer, and drug sensitivity testing is fundamental to this scheme. We aimed to develop an effective drug sensitivity test platform to support individualized treatment. We designed a microfluidic chip-based, three-dimensional (3D) co-culture drug sensitivity test platform. A mono-lung cancer cell line, a mixture of lung cancer and stromal cell lines, and cells from fresh lung cancer tissues were cultured in 3D under continuous media supplementation, mimicking the actual tumor microenvironment in vivo. The cells were treated with anti-cancer drugs according to a gradient concentration generator inside the chips to screen the appropriate chemotherapy schemes. We successfully cultured cell lines or primary cells with this device. We also smoothly assayed the sensitivities of different anti-cancer drugs in parallel and accurately screened appropriate-dose, single and combined-drug chemotherapy schemes for eight patients. Our microfluidic device is a simple, reliable, and high-throughput platform to test drug sensitivity. It would be possible for chemotherapists to screen the appropriate chemotherapy schemes to guide individualized treatment in lung cancer.</description><subject>3D co-culture</subject><subject>Advanced Basic Science</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Antineoplastic Agents - therapeutic use</subject><subject>Apoptosis - drug effects</subject><subject>Cell Line, Tumor</subject><subject>Cell Shape - drug effects</subject><subject>Chemotherapy</subject><subject>Coculture Techniques - methods</subject><subject>Dentistry</subject><subject>Drug Screening Assays, Antitumor</subject><subject>Drug sensitivity test</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - drug effects</subject><subject>Humans</subject><subject>Individualized treatment</subject><subject>Lung cancer</subject><subject>Lung Neoplasms - drug therapy</subject><subject>Lung Neoplasms - pathology</subject><subject>Microfluidic Analytical Techniques - methods</subject><subject>Microfluidic chip</subject><subject>Reproducibility of Results</subject><issn>0142-9612</issn><issn>1878-5905</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNksuKFTEQhoMoznH0FSS4ctNtLp2-uBCGuagw4EJdh3Slesyxu9Mm6YEjPozPMk9mmjOKuHIVCv6__tRXRcgLzkrOeP1qX_bOTyZhcGaMpWBclkyUrFIPyI63TVuojqmHZMd4JYqu5uKEPIlxz3LNKvGYnAhZNbKrxY78OFuW0YFJzs_UD9TQyUHww7g664DCF7cUvYloqbyg4AtYx7QGpMnThDFRG9YbGnGOLrlblw508OHup5ttruxqRvc9W1NAkyac05YwrvMNBTMDhqfk0ZAnwGf37yn5fHX56fxdcf3h7fvzs-sClGpTgapRIJpB9tbWPWfMVkpJWwECViCgl50SnWR9XbUVGG6ZgKEVUkHHMcORp-Tlse8S_Lc1f1tPLgKOo5nRr1FzyZtWsYqJLH19lGYIMQYc9BLcZMJBc6Y3-nqv_6avN_qaCZ3pZ_Pz-5y1n9D-sf7GnQUXRwHmaW8dBh3BYUZhXUBI2nr3fzlv_mkDo5vzFseveMC492uYNw_XMRv0x-0OtjPgkjEh2kb-Ag9WtKQ</recordid><startdate>20130501</startdate><enddate>20130501</enddate><creator>Xu, Zhiyun</creator><creator>Gao, Yanghui</creator><creator>Hao, Yuanyuan</creator><creator>Li, Encheng</creator><creator>Wang, Yan</creator><creator>Zhang, Jianing</creator><creator>Wang, Wenxin</creator><creator>Gao, Zhancheng</creator><creator>Wang, Qi</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130501</creationdate><title>Application of a microfluidic chip-based 3D co-culture to test drug sensitivity for individualized treatment of lung cancer</title><author>Xu, Zhiyun ; Gao, Yanghui ; Hao, Yuanyuan ; Li, Encheng ; Wang, Yan ; Zhang, Jianing ; Wang, Wenxin ; Gao, Zhancheng ; Wang, Qi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c558t-e575c27f3bdd6b100d4553d4cece4c2cb3952930b6484ca1d02cf8235c91e0133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>3D co-culture</topic><topic>Advanced Basic Science</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Antineoplastic Agents - therapeutic use</topic><topic>Apoptosis - drug effects</topic><topic>Cell Line, Tumor</topic><topic>Cell Shape - drug effects</topic><topic>Chemotherapy</topic><topic>Coculture Techniques - methods</topic><topic>Dentistry</topic><topic>Drug Screening Assays, Antitumor</topic><topic>Drug sensitivity test</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - drug effects</topic><topic>Humans</topic><topic>Individualized treatment</topic><topic>Lung cancer</topic><topic>Lung Neoplasms - drug therapy</topic><topic>Lung Neoplasms - pathology</topic><topic>Microfluidic Analytical Techniques - methods</topic><topic>Microfluidic chip</topic><topic>Reproducibility of Results</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Zhiyun</creatorcontrib><creatorcontrib>Gao, Yanghui</creatorcontrib><creatorcontrib>Hao, Yuanyuan</creatorcontrib><creatorcontrib>Li, Encheng</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Zhang, Jianing</creatorcontrib><creatorcontrib>Wang, Wenxin</creatorcontrib><creatorcontrib>Gao, Zhancheng</creatorcontrib><creatorcontrib>Wang, Qi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Zhiyun</au><au>Gao, Yanghui</au><au>Hao, Yuanyuan</au><au>Li, Encheng</au><au>Wang, Yan</au><au>Zhang, Jianing</au><au>Wang, Wenxin</au><au>Gao, Zhancheng</au><au>Wang, Qi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of a microfluidic chip-based 3D co-culture to test drug sensitivity for individualized treatment of lung cancer</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2013-05-01</date><risdate>2013</risdate><volume>34</volume><issue>16</issue><spage>4109</spage><epage>4117</epage><pages>4109-4117</pages><issn>0142-9612</issn><issn>1878-5905</issn><eissn>1878-5905</eissn><abstract>Abstract Individualized treatment is a promising clinical strategy for lung cancer, and drug sensitivity testing is fundamental to this scheme. We aimed to develop an effective drug sensitivity test platform to support individualized treatment. We designed a microfluidic chip-based, three-dimensional (3D) co-culture drug sensitivity test platform. A mono-lung cancer cell line, a mixture of lung cancer and stromal cell lines, and cells from fresh lung cancer tissues were cultured in 3D under continuous media supplementation, mimicking the actual tumor microenvironment in vivo. The cells were treated with anti-cancer drugs according to a gradient concentration generator inside the chips to screen the appropriate chemotherapy schemes. We successfully cultured cell lines or primary cells with this device. We also smoothly assayed the sensitivities of different anti-cancer drugs in parallel and accurately screened appropriate-dose, single and combined-drug chemotherapy schemes for eight patients. Our microfluidic device is a simple, reliable, and high-throughput platform to test drug sensitivity. 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| subjects | 3D co-culture Advanced Basic Science Antineoplastic Agents - pharmacology Antineoplastic Agents - therapeutic use Apoptosis - drug effects Cell Line, Tumor Cell Shape - drug effects Chemotherapy Coculture Techniques - methods Dentistry Drug Screening Assays, Antitumor Drug sensitivity test Fibroblasts - cytology Fibroblasts - drug effects Humans Individualized treatment Lung cancer Lung Neoplasms - drug therapy Lung Neoplasms - pathology Microfluidic Analytical Techniques - methods Microfluidic chip Reproducibility of Results |
| title | Application of a microfluidic chip-based 3D co-culture to test drug sensitivity for individualized treatment of lung cancer |
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