8-oxo-7,8-dihydroguanine level - the DNA oxidative stress marker - recognized by fluorescence image analysis in sporadic uterine adenocarcinomas in women
In the case of carcinogenesis in human endometrium no information exists on tissue concentration of 8-oxo-7,8-dihydroguanine, the DNA oxidative stress marker This was the main reason to undertake the investigation of this DNA modification in human uterine estrogen-dependent tissue cancers. In order...
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| Veröffentlicht in: | Ginekologia polska 2013-01, Vol.84 (1), p.44-50 |
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| description | In the case of carcinogenesis in human endometrium no information exists on tissue concentration of 8-oxo-7,8-dihydroguanine, the DNA oxidative stress marker This was the main reason to undertake the investigation of this DNA modification in human uterine estrogen-dependent tissue cancers.
In order to estimate the level of oxidative damage, 8-oxo-7,8-dihydroguanine was determined directly in cells of tissue microscope slides using OxyDNA Assay Kit, Fluorometric. Cells were investigated under confocal microscope. Images of individual cells were captured by computer-interfaced digital photography and analyzed for fluorescence intensities (continuous inverted 8-bit gray-scale = 0 [black]-255 [white]). Fluorescence scores were calculated for each of 13 normal endometrial samples and 31 uterine adenocarcinoma specimens. Finally the level of the oxidative stress marker was also analyzed according to histological and clinical features of the neoplasms.
The obtained data revealed that: 8-oxo-7,8-dihydroguanine levels were higher in uterine adenocarcinomas than in normal endometrial samples (48,32 vs. 38,64; p |
| doi_str_mv | 10.17772/gp/1539 |
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In order to estimate the level of oxidative damage, 8-oxo-7,8-dihydroguanine was determined directly in cells of tissue microscope slides using OxyDNA Assay Kit, Fluorometric. Cells were investigated under confocal microscope. Images of individual cells were captured by computer-interfaced digital photography and analyzed for fluorescence intensities (continuous inverted 8-bit gray-scale = 0 [black]-255 [white]). Fluorescence scores were calculated for each of 13 normal endometrial samples and 31 uterine adenocarcinoma specimens. Finally the level of the oxidative stress marker was also analyzed according to histological and clinical features of the neoplasms.
The obtained data revealed that: 8-oxo-7,8-dihydroguanine levels were higher in uterine adenocarcinomas than in normal endometrial samples (48,32 vs. 38,64; p<0,001); in contrast to normal endometrium there was no correlation between age and DNA oxidative modification content in uterine cancer; highest mean fluorescence intensity was recognized in G2 endometrial adenocarcinomas; level of 8-oxo-7,8-dihydroguanine does not depend on Body Mass Index (BMI) and cancer uterine wall infiltration or tumor FIGO stage.
Our study indicates that accumulation of the oxidized DNA base may contribute to the development of endometrial neoplasia, however oxidative DNA damage does not seem to increase with tumor progression.</description><identifier>ISSN: 0017-0011</identifier><identifier>EISSN: 0017-0011</identifier><identifier>EISSN: 2543-6767</identifier><identifier>DOI: 10.17772/gp/1539</identifier><identifier>PMID: 23488309</identifier><language>eng</language><publisher>Poland: Wydawnictwo Via Medica</publisher><subject>Adenocarcinoma - chemistry ; Adenocarcinoma - genetics ; Adenocarcinoma - ultrastructure ; Adult ; Aged ; Aged, 80 and over ; Biomarkers, Tumor - analysis ; Deoxyribonucleic acid ; DNA ; DNA Damage ; DNA, Neoplasm - analysis ; Endometrial Neoplasms - chemistry ; Endometrial Neoplasms - genetics ; Endometrial Neoplasms - ultrastructure ; Endometrium - ultrastructure ; Female ; Genetic Markers ; Guanine - analogs & derivatives ; Guanine - analysis ; Humans ; Middle Aged ; Optical Imaging - methods ; Oxidative Stress</subject><ispartof>Ginekologia polska, 2013-01, Vol.84 (1), p.44-50</ispartof><rights>2013. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c345t-c627f5913c98e3574173edddda92b4509de1ad2c973b2a35578bebbcb3dec40a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,4009,27902,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23488309$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Postawski, Krzysztof</creatorcontrib><creatorcontrib>Przadka-Rabaniuk, Dorota</creatorcontrib><creatorcontrib>Piersiak, Tomasz</creatorcontrib><title>8-oxo-7,8-dihydroguanine level - the DNA oxidative stress marker - recognized by fluorescence image analysis in sporadic uterine adenocarcinomas in women</title><title>Ginekologia polska</title><addtitle>Ginekol Pol</addtitle><description>In the case of carcinogenesis in human endometrium no information exists on tissue concentration of 8-oxo-7,8-dihydroguanine, the DNA oxidative stress marker This was the main reason to undertake the investigation of this DNA modification in human uterine estrogen-dependent tissue cancers.
In order to estimate the level of oxidative damage, 8-oxo-7,8-dihydroguanine was determined directly in cells of tissue microscope slides using OxyDNA Assay Kit, Fluorometric. Cells were investigated under confocal microscope. Images of individual cells were captured by computer-interfaced digital photography and analyzed for fluorescence intensities (continuous inverted 8-bit gray-scale = 0 [black]-255 [white]). Fluorescence scores were calculated for each of 13 normal endometrial samples and 31 uterine adenocarcinoma specimens. Finally the level of the oxidative stress marker was also analyzed according to histological and clinical features of the neoplasms.
The obtained data revealed that: 8-oxo-7,8-dihydroguanine levels were higher in uterine adenocarcinomas than in normal endometrial samples (48,32 vs. 38,64; p<0,001); in contrast to normal endometrium there was no correlation between age and DNA oxidative modification content in uterine cancer; highest mean fluorescence intensity was recognized in G2 endometrial adenocarcinomas; level of 8-oxo-7,8-dihydroguanine does not depend on Body Mass Index (BMI) and cancer uterine wall infiltration or tumor FIGO stage.
Our study indicates that accumulation of the oxidized DNA base may contribute to the development of endometrial neoplasia, however oxidative DNA damage does not seem to increase with tumor progression.</description><subject>Adenocarcinoma - chemistry</subject><subject>Adenocarcinoma - genetics</subject><subject>Adenocarcinoma - ultrastructure</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Damage</subject><subject>DNA, Neoplasm - analysis</subject><subject>Endometrial Neoplasms - chemistry</subject><subject>Endometrial Neoplasms - genetics</subject><subject>Endometrial Neoplasms - ultrastructure</subject><subject>Endometrium - ultrastructure</subject><subject>Female</subject><subject>Genetic Markers</subject><subject>Guanine - analogs & derivatives</subject><subject>Guanine - analysis</subject><subject>Humans</subject><subject>Middle Aged</subject><subject>Optical Imaging - methods</subject><subject>Oxidative Stress</subject><issn>0017-0011</issn><issn>0017-0011</issn><issn>2543-6767</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkc1O3DAUha2KqqDpSH2CyhKbLhrwX3C8RFB-pBFs6Dpy7DsZ08QOdjKd6Zvwthg6lIq7sC3dT-f63IPQF0qOqJSSHbfDMS25-oAOCKGyyAfd---9j-Yp3ZNcJ0wypT6hfcZFVXGiDtBjVYRNKOT3qrButbUxtJP2zgPuYA0dLvC4Anx-c4rDxlk9ujXgNEZICfc6_oKYiQgmtN79AYubLV52U8h9A94Adr1uAWuvu21yCTuP0xCits7gaYT4PEdb8MHoaJwPvX5hfoce_Gf0cam7BPPdPUM_L37cnV0Vi9vL67PTRWG4KMfCZFPLUlFuVAW8lIJKDjaXVqwRJVEWqLbMKMkbpnlZyqqBpjENt2AE0XyGvv3VHWJ4mCCNde_y77tOewhTqimnshJSSJLRw3fofZhiNpdqJk4Eo0SU9E3QxJBShGU9xLyHuK0pqV8Sq9uhfk4so193glPTg_0HvubDnwCafpIF</recordid><startdate>201301</startdate><enddate>201301</enddate><creator>Postawski, Krzysztof</creator><creator>Przadka-Rabaniuk, Dorota</creator><creator>Piersiak, Tomasz</creator><general>Wydawnictwo Via Medica</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>201301</creationdate><title>8-oxo-7,8-dihydroguanine level - the DNA oxidative stress marker - recognized by fluorescence image analysis in sporadic uterine adenocarcinomas in women</title><author>Postawski, Krzysztof ; Przadka-Rabaniuk, Dorota ; Piersiak, Tomasz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c345t-c627f5913c98e3574173edddda92b4509de1ad2c973b2a35578bebbcb3dec40a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adenocarcinoma - chemistry</topic><topic>Adenocarcinoma - genetics</topic><topic>Adenocarcinoma - ultrastructure</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Damage</topic><topic>DNA, Neoplasm - analysis</topic><topic>Endometrial Neoplasms - chemistry</topic><topic>Endometrial Neoplasms - genetics</topic><topic>Endometrial Neoplasms - ultrastructure</topic><topic>Endometrium - ultrastructure</topic><topic>Female</topic><topic>Genetic Markers</topic><topic>Guanine - analogs & derivatives</topic><topic>Guanine - analysis</topic><topic>Humans</topic><topic>Middle Aged</topic><topic>Optical Imaging - methods</topic><topic>Oxidative Stress</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Postawski, Krzysztof</creatorcontrib><creatorcontrib>Przadka-Rabaniuk, Dorota</creatorcontrib><creatorcontrib>Piersiak, Tomasz</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Ginekologia polska</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Postawski, Krzysztof</au><au>Przadka-Rabaniuk, Dorota</au><au>Piersiak, Tomasz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>8-oxo-7,8-dihydroguanine level - the DNA oxidative stress marker - recognized by fluorescence image analysis in sporadic uterine adenocarcinomas in women</atitle><jtitle>Ginekologia polska</jtitle><addtitle>Ginekol Pol</addtitle><date>2013-01</date><risdate>2013</risdate><volume>84</volume><issue>1</issue><spage>44</spage><epage>50</epage><pages>44-50</pages><issn>0017-0011</issn><eissn>0017-0011</eissn><eissn>2543-6767</eissn><abstract>In the case of carcinogenesis in human endometrium no information exists on tissue concentration of 8-oxo-7,8-dihydroguanine, the DNA oxidative stress marker This was the main reason to undertake the investigation of this DNA modification in human uterine estrogen-dependent tissue cancers.
In order to estimate the level of oxidative damage, 8-oxo-7,8-dihydroguanine was determined directly in cells of tissue microscope slides using OxyDNA Assay Kit, Fluorometric. Cells were investigated under confocal microscope. Images of individual cells were captured by computer-interfaced digital photography and analyzed for fluorescence intensities (continuous inverted 8-bit gray-scale = 0 [black]-255 [white]). Fluorescence scores were calculated for each of 13 normal endometrial samples and 31 uterine adenocarcinoma specimens. Finally the level of the oxidative stress marker was also analyzed according to histological and clinical features of the neoplasms.
The obtained data revealed that: 8-oxo-7,8-dihydroguanine levels were higher in uterine adenocarcinomas than in normal endometrial samples (48,32 vs. 38,64; p<0,001); in contrast to normal endometrium there was no correlation between age and DNA oxidative modification content in uterine cancer; highest mean fluorescence intensity was recognized in G2 endometrial adenocarcinomas; level of 8-oxo-7,8-dihydroguanine does not depend on Body Mass Index (BMI) and cancer uterine wall infiltration or tumor FIGO stage.
Our study indicates that accumulation of the oxidized DNA base may contribute to the development of endometrial neoplasia, however oxidative DNA damage does not seem to increase with tumor progression.</abstract><cop>Poland</cop><pub>Wydawnictwo Via Medica</pub><pmid>23488309</pmid><doi>10.17772/gp/1539</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adenocarcinoma - chemistry Adenocarcinoma - genetics Adenocarcinoma - ultrastructure Adult Aged Aged, 80 and over Biomarkers, Tumor - analysis Deoxyribonucleic acid DNA DNA Damage DNA, Neoplasm - analysis Endometrial Neoplasms - chemistry Endometrial Neoplasms - genetics Endometrial Neoplasms - ultrastructure Endometrium - ultrastructure Female Genetic Markers Guanine - analogs & derivatives Guanine - analysis Humans Middle Aged Optical Imaging - methods Oxidative Stress |
| title | 8-oxo-7,8-dihydroguanine level - the DNA oxidative stress marker - recognized by fluorescence image analysis in sporadic uterine adenocarcinomas in women |
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